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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for Recombinant Human IL6 Protein non tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... induction of AID-tagged protein depletion was performed with 3-indole-acetic acid (IAA) (Sigma-Aldrich) during the exponential growth phase (approx ...
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bioRxiv - Neuroscience 2022Quote: ... To immunoprecipitate FLAG-tagged proteins the supernatant was incubated with anti-FLAG M2 Affinity Gel (Sigma) for 1h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were detected by anti-HA mAb (clone HA-7, 1:5000, Sigma-Aldrich); NSP5 and VP2 proteins were detected using anti-NSP5 guinea pig serum (1:5000 ...
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bioRxiv - Molecular Biology 2019Quote: Purified FLAG-tagged CSB proteins were immobilized on pre-equilibrated Anti-FLAG M2 Magnetic Beads (Sigma) for 2 h at 4°C ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Genetics 2020Quote: ... Epitope-tagged proteins were immunoprecipitated with either anti-Flag- or anti-HA-coated magnetic beads (Sigma and Pierce ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG-tagged proteins were eluted from the beads with 200 µg/ml FLAG peptide (Sigma-Aldrich) in 0.5 ml of lysis buffer B ...
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bioRxiv - Genetics 2023Quote: ... Proteins tagged with 6His10FLAG were detected by immunoblot-ting using an anti-FLAG mouse antibody (Sigma ANTI-FLAG M2 antibody F3165 ...
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bioRxiv - Plant Biology 2023Quote: ... The HAHis-tagged SbtB proteins were detected with monoclonal antibodies against the HA-epitope (Sigma, USA). Secondary ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-tagged proteins were eluted from the beads using 150 ng/ml of 3xFLAG peptide (Sigma) in 50–100 ul of elution buffer (20 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Genetics 2023Quote: ... and samples were analyzed by standard immunoblotting procedures using 1:3000 peroxydase-conjugated antiperoxydase (PAP, to detect protein-A- tagged proteins) (Sigma, #P1291, RRID:AB_1079562), 1:3000 monoclonal anti-FLAG antibody (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35 days ...
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bioRxiv - Microbiology 2020Quote: ... were treated with or without 25-100 U/mL of human recombinant IFN-α (Sigma-Aldrich) for 4-12 hours after which total RNA was isolated and subjected to qRT-PCR in order to measure expression of ISGs.
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bioRxiv - Microbiology 2019Quote: ... EVs were incubated with Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) at different final concentration (0 to 10 µg/ml) ...
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bioRxiv - Microbiology 2021Quote: For expression of C13/N15 labeled recombinant human wildtype-MIF BL21-(DE3)-Rosetta-pLysS bacteria (Novagen) were transformed with pET11a-MIF and spread on LB-Agar plates containing 50 µg/mL carbenicillin and 34 µg/mL chloramphenicol ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 µg/ml BSA fraction V and 10 µg/ml human recombinant in sulin (both Sigma)) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human EGF (Cat# E9644) and Glucose Quantification Kit (Cat# GAGO-20) purchased from Sigma-Aldrich; Cisplatin (Cat# 232120 ...
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bioRxiv - Microbiology 2019Quote: ... Glutamax and Pen/Strep and with 100 U/mL recombinant human IL-2 (Roche; Sigma # 10799068001). For the analysis of reverse transcription products ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35±3 days25 ...
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bioRxiv - Physiology 2023Quote: ... and recombinant human interleukin-1β (IL-β) were obtained from Millipore Sigma (St Louis, MO, USA). Thapsigargin (TG ...
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bioRxiv - Molecular Biology 2023Quote: ... TGFB1 signaling was induced with 5 ng/ml final concentration of human recombinant TGFB1 (Sigma-Aldrich) for 4 h.
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bioRxiv - Biophysics 2021Quote: The human esaHis-tagged PTPN11 (residues 1-528) cDNA was cloned in a pET-26b vector (Novagen, MA, USA). Nucleotide substitutions associated with NS or leukemia were introduced by site-directed mutagenesis (QuikChange site-directed mutagenesis kit ...
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bioRxiv - Genetics 2023Quote: Human mCherry-tagged CTNS mRNA solutions were prepared in RNAse free water (500ng/µl) with 0.05% PhenolRed (Sigma, cat.no.P0290). All mRNA injections were performed at the one-cell stage using a FemtoJet microinjector (Thermofisher ...
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bioRxiv - Microbiology 2020Quote: ... Biotinylated proteins were captured in ELISA plates pre-coated with 5 µg/mL of Avidin (Sigma) in Na2CO3/NaHCO3 buffer pH 9.6 ...
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bioRxiv - Physiology 2022Quote: ... Hyaluronic acid/hyaluronan (HA) was stained overnight using biotinylated HA binding protein (Millipore, Burlington, MA; 385911). All sections were washed and incubated with VECTASTAIN® ABC-HRP kit (Vector laboratories ...
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bioRxiv - Bioengineering 2020Quote: ... or human hemoglobin (all proteins were purchased from Sigma). Protein nanorods were prepared using chemically modified human serum albumin.
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bioRxiv - Cancer Biology 2020Quote: ... For some analyses mammosphere media was supplemented additionally with 10 ng/ml IL6 (Sigma-Aldrich, Germany), 1.5 µg/ml anti-IL6 antibody (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Plasma samples from four Danish non-pregnant women without malaria exposure and purified human IgG (Sigma-Aldrich) were included as negative controls.
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bioRxiv - Microbiology 2020Quote: ... the Milliplex® Map Non-Human Primate Cytokine Magnetic Bead Panel Kit (EMD Millipore Corporation, Billerica, MA) was used ...
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bioRxiv - Neuroscience 2020Quote: ... remaining archived samples were processed using a non-human primate multiplexing bead immunoassay (Milipore-Sigma, Burlington, MA). The kit was run according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... or a non-target negative control gRNA (Human Sanger Arrayed Whole Genome Lentiviral CRISPR library, Sigma-Aldrich) using polyethylenimine (PEI ...
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bioRxiv - Cancer Biology 2021Quote: ... lysates were clarified and Flag-tagged protein were precipitated using anti-Flag M2 magnetic beads (Sigma Aldrich). After several washes in IP buffer ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were purified using anti-HA affinity matrix (Sigma; rat anti-HA clone 3F10 antibodies) and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma ...
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bioRxiv - Genetics 2020Quote: ... FLAG-tagged LIR-2 protein was detected with monoclonal anti-FLAG M2-Peroxidase (HRP) antibody (SIGMA-Aldrich) and EGFP:NHR-25 was visualized with Clarity Western ECL substrate (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Biochemistry 2022Quote: ... Flag-tagged RtcB proteins were affinity-purified from the lysates with anti-Flag M2 affinity-gel (Sigma) followed by extensive washes ...
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bioRxiv - Biochemistry 2021Quote: ... Beads were washed three times and FLAG-tagged proteins eluted using 3X FLAG peptide (Sigma; cat. F4799). Rhodamine-Azide labeling reactions were performed using 1.7 mM TBTA ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG-tagged proteins were detected with horseradish peroxidase (HRP)-conjugated anti-FLAG antibody (Sigma Aldrich, A8592-1MG). All western blots were developed using the ECL Plus Western Blotting Detection System (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... The FLAG-tagged PCRK2 and PBL19 proteins were immunoprecipitated using 15 μl M2 beads (Sigma; Cat. #A2220). Biotinylation was detected with Streptavidin-HRP (Abcam Cat ...
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bioRxiv - Plant Biology 2021Quote: ... The FLAG-tagged NRG1C and SAG101 proteins were immunoprecipitated using 20 µl M2 beads (Sigma; Cat. #A2220) for co-immunoprecipitation and biotinylation was detected by Streptavidin-HRP (Abcam Cat ...
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bioRxiv - Microbiology 2020Quote: ... HA3-tagged proteins were detected using a monoclonal rat α-HA3 (1:1000; clone 3F10; Sigma-Aldrich). Actin ...
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bioRxiv - Biochemistry 2022Quote: ... and HA-tagged full length CASK proteins were incubated with 1μg anti-Flag M2 antibody (Sigma, F3165) at 4°C over night ...
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bioRxiv - Cell Biology 2022Quote: Flag-tagged proteins were captured from 1 mg total cell lysate using anti-Flag affinity matrix (Sigma) for 1 hour at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... HA-tagged proteins were detected with an Anti-HA-Peroxidase antibody (Sigma-Aldrich, St. Louis, USA, 12013819001). For the RACB ubiquitination screening ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitation of Flag-tagged proteins were carried out with the anti-FLAG M2 beads (Cat. A2220, Sigma) in RIPA buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... 6×His-tagged protein were expressed and purified by Ni-NTA His Bind Resin (Millipore 70666-3). ∼0.2 μg of GST-OsBZR1 ...
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bioRxiv - Plant Biology 2021Quote: ... Flag-tagged proteins were detected using the anti-Flag HRP-coupled antibody (mouse monoclonal, A8592; Sigma-Aldrich) diluted 1:5000 (v/v) ...
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bioRxiv - Cell Biology 2022Quote: ... Immunodetection of ProteinA (ProtA)-tagged proteins was performed using Peroxidase Anti-Peroxidase Soluble Complex antibody (Sigma-Aldrich) in a 1:3,000 dilution ...