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Citations for Millipore Sigma :
301 - 350 of 3267 citations for Recombinant Human DCLK1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: ... The recombinant protein was produced in Rosetta (DE3) cells (Novagen) and purified by affinity chromatography using a GSTrapFF column ...
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bioRxiv - Pathology 2021Quote: ... and 1,000 U of recombinant leukemia inhibitory factor (ESGRO; Millipore) on mitotically inactivated feeder cells as described (Hughes and Saunders ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant mTOR was purchased from Millipore (14-770) and recombinant 4EBP1 was purchased from Millipore (516678). Flag-STK11IP or 4EBP1 was incubated with or without mTOR in 50 μl reaction mixtures at 37°C for 60 mins ...
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bioRxiv - Microbiology 2021Quote: Recombinant proteins were created using the pET expression system (Novagen). The codon-optimized typical faeG gene of the original K88 (K88ac variant ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng recombinant His-AKT1 (inactive, Upstate/Millipore #14-279), at a final volume of 30 μl ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant protein was induced with 1 mM IPTG (Sigma – Aldrich) and the culture was transferred to 25°C and 250 rpm for 5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... active recombinant proteins were purchased from Sigma (SRP0364, P0066, respectively). Recombinant purified human MASTL protein was used as a substrate and was from Abnova (H00084930) ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant ubiquitin was purchased from Sigma Aldrich (79586-22-4).
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bioRxiv - Cancer Biology 2021Quote: ... Recombinant CDK5/p25 (60 ng) (EMD Millipore, Burlington, MA, USA) was combined with purified ACSS2 (2ug ...
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bioRxiv - Genetics 2022Quote: ... and 10 ug/L recombinant leukemia inhibitory factor (LIF) (Millipore). Mouse embryo stem cells was cultured at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... 25 ng/ml recombinant basic fibroblastic growth factor (FGF, Millipore), and 100 U/mL of penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant proteins were expressed in either BL21 or Rosetta (Novagen) cells ...
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bioRxiv - Biochemistry 2020Quote: Recombinant Ded1-His was expressed from the pET22b plasmid (Novagen) and purified as previously described (46) ...
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bioRxiv - Molecular Biology 2021Quote: Recombinant proteins were expressed in Escherichia coli RosettaTM (DE3) (Millipore) by induction overnight with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich), and 0.5 μg/ml anti-CD180 (RP/14 ...
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bioRxiv - Immunology 2020Quote: ... then stimulated with 100U/mL recombinant murine IFNβ (Sigma, I9032) or equivalent volume of water for 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... 10 µl recombinant RNase inhibitor (1 U/µl, Sigma-Aldrich) was added to 390 µl internal solution to increase RNA yield ...
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bioRxiv - Synthetic Biology 2023Quote: ... 103 units/mL ESGRO Recombinant Mouse LIF Protein (Millipore #ESG1107), 3.0 µM CHIR99021 (GSK-3 inhibitor ...
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bioRxiv - Molecular Biology 2023Quote: ... LPA and recombinant Src protein were purchased from Sigma-Aldrich, while recombinant human IDO1 protein was obtained by Giotto Biotech ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/ml mouse recombinant IL-4 (Sigma-Aldrich) to allow B cell activation and class switch recombination to IgG1 ...
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bioRxiv - Bioengineering 2023Quote: ... recombinant insulin-like growth factor (R3-IGF) (Sigma Aldrich, #85580C), heparin (Sigma Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... All recombinant plasmids were transformed into E.coli Rosetta (DE3) (Novagen) cells and grown in LB broth at 37°C for 3 h ...
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bioRxiv - Developmental Biology 2023Quote: ... 1000 U/ml ESGRO recombinant mouse LIF protein (Sigma ESG1106), 2 μM PD0325901 (Selleckchem S1036 ...
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bioRxiv - Immunology 2023Quote: ... Anti-human IgG HRP antibody (Anti-Human IgG-Peroxidase from Sigma) was diluted 1:30,000 in blocking buffer ...
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bioRxiv - Bioengineering 2022Quote: ... human iPSC-derived Brain Microvascular Endothelial-like cells were seeded in the vascular channel at a density of 14 to 16×106 cells/mL using human serum-free endothelial cell medium supplemented with 5% human serum from platelet-poor human plasma (Sigma) and allowed to attach to the membrane overnight ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of biotinylated secondary antibody diluted in PBS-BN (anti-human IgG, B1140, 1/1250; anti-human IgM, B1265, 1/1000; or anti-human IgA, SAB3701227, 1/500; Sigma) were added to all wells and incubated for 45 min at 600 rpm at RT and protected from light ...
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bioRxiv - Immunology 2022Quote: Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... Recombinant GST-p50 subunit D434-969 was purchased from Sigma-Aldrich. Binding reactions were performed for 20 minutes at room temperature with 20 pmol annealed primers ...
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bioRxiv - Immunology 2021Quote: Recombinant cytokines were purchased from indicated suppliers: IFN-β1a (Sigma, #IF014); IFN-γ (Sigma ...
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bioRxiv - Genomics 2020Quote: ... 1000 U/ml recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore), and 50U/ml Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2019Quote: ... Following 12 h recombinant expression in Rosetta Competent Cells (Millipore, #70954) at 12° C with ampicillin and chloramphenicol selection ...
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bioRxiv - Cell Biology 2019Quote: ... 1000 U/mL mouse recombinant Leukemia inhibitory factor (LIF, ESG1107, Millipore) and Penicillin-Streptomycin-Neomycin Antibiotic Mixture (15640055 ...
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bioRxiv - Biophysics 2021Quote: Recombinant HSA expressed in Pichia pastoris was purchased from Sigma-Aldrich (#A7736 ...
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bioRxiv - Neuroscience 2021Quote: ... and with or without 0.3 µM recombinant BirA (Sigma-Aldrich; SRP0417) (Howarth and Ting ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant proteins were induced in Rosetta™ (DE3) Competent Cells (Novagen) with 0.02% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Systems Biology 2020Quote: ... Recombinants were selected on LB-agar containing 15μg/ml kanamycin (Sigma). Successful integration was confirmed via amplification and sequencing of the flu locus ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant TNFα was purchased from Sigma-Aldrich (St. Louis, MO, USA). The protease inhibitor cocktail was purchased from Thermo Fisher Scientific (MA ...
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bioRxiv - Biochemistry 2022Quote: ... Standard curves were generated using recombinant HIV-1 RT (Merck Millipore). The relative viral quantities were calculated based on the standard curve generated using QuantStudio-7 systems software (Applied Biosystems).
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bioRxiv - Plant Biology 2022Quote: PIF3 recombinant proteins were prepared using PIF3-his DNA (pET28C, Novagen) provided by Ferenc Nagy and induced and purified using commercial kit (Amersham) ...
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bioRxiv - Cell Biology 2022Quote: For binding assays with recombinant KAP (Sigma Aldrich, Cat. No. SRP5175) or CAK (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... To induce recombinant proteins isopropyl-β-D-thiogalactoside (IPTG, Sigma-Aldrich) was added (0.1 mM ...
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bioRxiv - Immunology 2022Quote: ... cells were then stimulated with 100U/ml recombinant IFNα2 (Sigma-Aldrich) to induce transcription from the IFIT1 loci ...
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bioRxiv - Biochemistry 2022Quote: ... 1000 U/mL recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore) and 50 U/mL Penicillin–Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was eluded with 250 mM imidazole (Sigma).
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bioRxiv - Cell Biology 2021Quote: Recombinant protein expression vectors were constructed from the pET28a series (Novagen) encoding an N-terminal hexa-histidine tag and thrombin cleavage site.
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bioRxiv - Biochemistry 2020Quote: ... Recombinant rabbit anti-pan-ADP-ribose binding reagent MABE1016 (Merck Millipore) was used 1:1000 ...
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bioRxiv - Immunology 2020Quote: ... and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich) for CSR to IgG1 ...
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bioRxiv - Genomics 2022Quote: ... 1000 U/ml recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore), and 50U/ml Penicillin/Streptomycin (Thermo Fisher Scientific) ...