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Citations for Millipore Sigma :
201 - 250 of 9836 citations for Recombinant Human CD3D Flag and Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged protein was detected by incubating the milk powder-blocked membrane with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich) diluted at 1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of 3x-FLAG tagged S was performed with 1:400 monoclonal ANTI-FLAG® M2 antibody [F3165] produced in mouse (Sigma). 1:50,000 Alexa Fluor® 680 AffiniPure Goat Anti-Mouse IgG [115-625-174] (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated goat anti-human IgG Fc (Sigma-Aldrich) was used for detection ...
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bioRxiv - Microbiology 2020Quote: ... anti-human Fc-conjugated to alkaline phosphatase (A9544, Sigma) was added (50 µl/well ...
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SIMON, an automated machine learning system reveals immune signatures of influenza vaccine responsesbioRxiv - Immunology 2019Quote: ... supplemented with 10% FCS and human serum AB (Sigma) for 2 days at 107 cells/ml in 24-well plate following “RESTORE” protocol (67 ...
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bioRxiv - Immunology 2020Quote: ... goat anti-human IgG (Fc) peroxidase conjugate (Sigma A0170) for 45 min ...
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bioRxiv - Immunology 2021Quote: ... using an anti-human Fc mAb (Sigma-Aldrich, I6260) as capture ...
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bioRxiv - Developmental Biology 2021Quote: ... We injected in each matrix 25 μg of Recombinant Sema3C-Fc (Sigma-Aldrich) in a concentration of 50 μg/ml in PBS ...
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bioRxiv - Genetics 2021Quote: ... 10 μg ml-recombinant human insulin (Sigma, 19278-5ML), 2 IU ml-heparin (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... ErbB2 (Her2) human recombinant was purchased from Sigma-Aldrich. Deglycosylated trastuzumab was obtained by treatment with Endo S (New England BioLabs ...
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bioRxiv - Cell Biology 2021Quote: ... 10 nM recombinant human [Leu15]-gastrin I (Sigma G9145), 50 ng/ml recombinant mouse EGF (Peprotech ...
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bioRxiv - Genomics 2019Quote: ... 100 U/mL recombinant human IL-2 (Sigma-Aldrich) and 1% penicillin-streptomycin (final concentration 100 U penicillin ...
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bioRxiv - Genomics 2020Quote: ... 100 U/mL recombinant human IL-2 (Sigma-Aldrich) and penicillin-streptomycin (final concentration 100 U penicillin ...
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bioRxiv - Cell Biology 2020Quote: ... recombinant human IGF1 (Sigma Chemicals, St. Louis, MO, USA), or glucose.
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bioRxiv - Neuroscience 2023Quote: ... Tau ladder (six human tau recombinant isoforms, Sigma-Merck) was used to identify tau isoforms in NCI-H716 cells ...
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bioRxiv - Genetics 2023Quote: ... 10 μg/ml recombinant human insulin (Sigma, 19278-5ML), 2 IU/ml heparin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... human recombinant insulin at 0.01 mg/mL (Sigma, I9278), and 1% penicillin-streptomycin mix ...
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bioRxiv - Immunology 2023Quote: ... Recombinant human (rh) Interleukin-2 (IL-2, Sigma-Aldrich), rhTNFα (Peprotech ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.5 mg/ml human recombinant albumin (Sigma Aldrich, A9731) and 0.2 mg/ml L-ascorbic acid 2-phosphate (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2.5 μg/ml human recombinant insulin (Sigma, 19278). Wide-bore P1000 pipette tips with ∼3 mm diameter openings were used to transfer tumor pieces ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 mIU/ml recombinant human chorionic gonadotropin (hCG) (Sigma), 500 ng/ml of A4 (Sigma) ...
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bioRxiv - Bioengineering 2024Quote: ... 10 nM recombinant human (Leu15)-gastrin I (Sigma; G9145), 50 ng/mL recombinant human EGF ...
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bioRxiv - Neuroscience 2021Quote: UBQLN2 complexes were isolated from HEK293 cells overexpressing FLAG-tagged wild-type UBQLN2 using anti-FLAG M2 Affinity Gel (Millipore-Sigma, A2220). After washing ...
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bioRxiv - Biochemistry 2022Quote: ... and another was loaded with 10 ng of human HSP70 (His tagged human HSP70, SRP5190, Millipore Sigma), which allowed comparisons to be made among separate gels ...
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bioRxiv - Biochemistry 2020Quote: ... Goat anti-human IgG secondary antibody-Peroxidase (Fc-specific, Sigma) prepared at 1:3000 in PBST was then added and plates were incubated for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... plates were coated with anti–human Fc (Sigma, Catalog # FAB3700259) and goat anti–human HRP–conjugated IgG (H+L ...
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bioRxiv - Immunology 2023Quote: ... FCS was replaced with 5% human serum (Sigma-Aldrich, H6914). Hypersalinity (high NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... RPS2-Flag tagged 40S ribosomes were immuno-precipitated by addition of 50 µL washed anti-Flag M2 affinity agarose gel (Sigma Aldrich, A2220-5mL) and 5 µL RNasin Plus (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... The overexpressed 3X FLAG-tagged cyclin-Cdk1 complexes were then purified by immunoaffinity chromatography using ANTI-FLAG M2 affinity agarose beads (Sigma-Aldrich Cat #A2220) and eluted with 0.2 mg/mL 3X FLAG peptide (Sigma-Aldrich Cat #F4799) ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant SBF or MBF complex was purified with anti-FLAG resin (M2, Sigma) from insect cells co-infected with FLAGSwi4-Swi6 or FLAGMbpl-Swi6 baculovirus constructs ...
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Tuning trophoblast invasion in a gelatin hydrogel via soluble cues from the maternal-fetal interfacebioRxiv - Bioengineering 2020Quote: ... and recombinant human epidermal growth factor (EGF; Sigma-Aldrich, E9644) were added to the medium at a concentration of 5 ng/mL ...
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bioRxiv - Bioengineering 2022Quote: ... recombinant human type I or type III collagen (Millipore Sigma) were immobilized on a CM5 sensor chip (Cytiva ...
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bioRxiv - Bioengineering 2021Quote: ... and brain-derived neurotrophic factor (BDNF, human recombinant, Sigma-Aldrich). Concentrations used were in the range 50-100 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... and 30 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37°C for 4 days in a humidified CO2 incubator ...
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bioRxiv - Immunology 2021Quote: ... and 10 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37° C for 3 days in a humidified CO2 incubator.
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bioRxiv - Biophysics 2020Quote: ... 100 nM recombinant human EGF (Sigma-Aldrich, St. Luis, MS). For LatA treatments 1 µM LatA was added to cells and incubated for 10 min at 37°C prior to imaging ...
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bioRxiv - Immunology 2019Quote: ... and 10 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37°C for 3 days in a humidified CO2 incubator.
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bioRxiv - Immunology 2019Quote: ... and 30 U/ml recombinant human IL-2 (Sigma, 11011456001), and incubated at 37°C for 4 days in a humidified CO2 incubator.
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bioRxiv - Physiology 2020Quote: ... 5 μg/mL recombinant human insulin (Sigma-Aldrich, Dorset, UK), 0.5 ng/mL recombinant human basic fibroblast growth factor ...
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bioRxiv - Immunology 2020Quote: ... 300 U/mL Roche recombinant human IL-2 (Sigma-Aldrich) and 1 μg/mL phytohaemagglutinin (PHA ...
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bioRxiv - Immunology 2020Quote: ... and recombinant human RAP (Merck Millipore, 200 nM final concentration) were used to stimulate nuclear translocation of MerTK.
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bioRxiv - Molecular Biology 2021Quote: ... 5 ng/ml human recombinant epidermal growth factor (Sigma, UK) and 25 μg/ml fetuin from FBS (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... 100 ng/ml of recombinant human bFGF (F0291, Sigma-Aldrich, 50 μg/mL stock solution in 20 mM Tris pH=7.5 + BSA 0.1 % ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/ml recombinant human IGF-1 (85580C, Sigma-Aldrich), and 20 ng/ml human bFGF (PHG0021 ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg/mL human recombinant insulin (Sigma-Aldrich, 91077C).
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bioRxiv - Genetics 2024Quote: ... 10 μg/mL recombinant human insulin (Sigma Aldrich #I9278-5ml), and 1% penicillin-streptomycin (Thermo Scientific #15140122) ...
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bioRxiv - Biochemistry 2020Quote: ... for mFc tagged antigen versions or goat-anti-hIgG(Fc) conjugated with HRP (1:70000, A0170, Sigma) if hFc-tagged antigens had to be detected ...
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bioRxiv - Biochemistry 2020Quote: 3XFLAG-tagged Nth1 was immunoprecipitated using Anti-FLAG™ M2 magnetic beads (Sigma, product number: M8823). Magnetic beads were washed three times with wash buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... Affinity purification of tagged RPL18-containing polysomes was carried out using anti-FLAG beads (Sigma-Aldrich) incubated with the supernatant at 4 °C for 3 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... Sequences were inserted into the Bgl Ⅱ/Acc65I (TOYOBO) digested FLAG-tagged vector pCMV-FLAG4 (Sigma). pCMV-FLAG4 was used as a control.