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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for Recombinant Human CD3D & CD3E Flag Fc & His Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and/or 2.5 μg/ml E-cadherin-Fc (Sigma). IELs were plated in RPMI-T medium containing 2 μg/ml PerCP-e710 conjugated anti-CD107a (1D4B ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma Aldrich), 100 μg/ml streptomycin and 100 U/ml penicillin (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... and 10% FCS (Sigma-Aldrich, St. Louis, Missouri, USA)) assuming that they double within 24 hours ...
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bioRxiv - Immunology 2023Quote: ... mixed in 2% fetal calf serum (FCS; Sigma-Aldrich). Cells were stained with combinations of the following fluorophore-conjugated anti-mouse monoclonal antibodies ...
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bioRxiv - Immunology 2023Quote: ... 10% fetal calf serum (FCS) treated with Chelex (Sigma) to removed endogenous calcium ...
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bioRxiv - Microbiology 2023Quote: ... and 1% FCS (Sigma Aldrich, St. Louis, MO, USA). Thereafter ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich), 10 mM HEPES (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich) and 2 mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The cell suspension was incubated with 10% FCS (Sigma) for 30 min to allow the cell membrane to recover from trypsinisation and prevent false positive staining with Annexin V (AV) ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma Aldrich). All cells were kept at 37 °C with 5% CO2 and tested for mycoplasma at regular intervals.
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bioRxiv - Cell Biology 2024Quote: ... Anti-mouse IgG (Fc) TRITC (Sigma #SAB3701020, 1:500), Anti-rabbit IgG FITC (Sigma #F7512 ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% FCS (v/v) (Sigma- Aldrich Ltd), apart from T2 cells which were grown in glutamine containing RPMI (Sigma-Aldrich Ltd) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10% tetracycline-free fetal calf serum (FCS) (Sigma-Aldrich), 100 U/ml penicillin (Biochrom) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated FCS (Sigma Aldrich #F2442) and 50 ng/ml M-CSF (PeproTech #300-25 ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% fetal bovine serum (FCS, Sigma-Aldrich), 100 U/ml penicillin (ThermoFisher) ...
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bioRxiv - Genomics 2024Quote: ... containing 10% FCS (heat inactivated) (Sigma-Aldrich, Cat# F7524) and 0.5% penicillin and streptomycin at 27°C.
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bioRxiv - Microbiology 2022Quote: ... 10nM recombinant human gastrin (Sigma), 50ng/mL EGF (Peprotech) ...
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bioRxiv - Microbiology 2022Quote: ... 10nM recombinant human gastrin (Sigma), 50ng/mL EGF (Peprotech) ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated mouse anti-FLAG tag Ab (Sigma-Aldrich) was used to detect Fab/VH binding ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies to Flag-tag (F3165) were purchased from Sigma. Antibodies to pT308-Akt (ab38449 ...
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tag (M2) and α-Tubulin (T5168) from Sigma, RFP (600-401-379 ...
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal Flag-tag antibody (Sigma, F3165, 1:1000), rabbit monoclonal HA-tag antibody (CST ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-Flag tag (Sigma cat# F1804, 1:1000), rabbit anti-human actin (Sigma cat#A2066 ...
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bioRxiv - Neuroscience 2021Quote: ... The FLAG tag oligonucleotide was synthesized by Sigma-Aldrich as two complementary single-stranded oligonucleotides (Supplementary Table 1).
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bioRxiv - Neuroscience 2022Quote: ... The Flag-tag protein affinity sequence (DYKDDDDK; Sigma-Aldrich) was added to the N-terminal to facilitate confirmation of expression ...
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bioRxiv - Immunology 2023Quote: ... Antibody for Flag-Tag (A8592) was ordered from Sigma.
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Microbiology 2022Quote: ... with the following modifications: Mouse anti-GST tag or mouse anti-His tag 1:200× (Sigma-Aldrich) and HRP-conjugated goat anti-mouse IgG 1:2,000× (Seracare Life Sciences ...
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bioRxiv - Biochemistry 2024Quote: ... pCD44 was generated by pretreatment of recombinant murine CD44-Fc-AviTag with CK2α in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL). DNA was harvested from the libraries before and after sorting using Zymoprep Yeast Plasmid Miniprep II (Zymo Research ...
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Structure and Neutralization Mechanism of a Human Antibody Targeting a Complex Epitope on Zika VirusbioRxiv - Microbiology 2022Quote: ... Plates were washed with TBS containing 0.2% Tween and incubated for 1 hour at 37°C with Goat anti-human Fc (Sigma, A9544) or Fab (Jackson immunoResearch AB_2337617 ...
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bioRxiv - Cell Biology 2021Quote: ... plates were washed 4 times with PBST and further incubated with 100 μL per well of goat anti-human IgG (Fc specific)-Peroxidase antibody (1: 5000 dilution, Sigma) for 1 h at 37°C ...
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bioRxiv - Microbiology 2020Quote: rAkata EBV-infected cells was reactivated at 1×106 cells/mL with a goat polyclonal anti-human IgG Fc-specific antibody (Sigma) for 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... the plate was washed 5 times with PBS and incubated for 1 hour with goat anti-human IgG (Fc specific) –peroxidase (#A0170, Sigma). Binding signals were developed and stopped by the addition of TMB (3,3’,5,5’-tetramethylbenzidine ...
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bioRxiv - Evolutionary Biology 2020Quote: ... complete His-Tag Purification Resin (NiNTA) were purchased from Sigma Aldrich. Protein inhibitor cocktail (PIC ...
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bioRxiv - Biochemistry 2021Quote: ... anti-TtSmtB antibodies (GeneCust™) and His-tag antibodies (Sigma-Aldrich). His-tag removal from recombinant TtSmtB was performed as previously described (22).
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bioRxiv - Cell Biology 2021Quote: ... His-tag was removed using an Enterokinase Cleavage Capture kit (Novagen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... His-tag removal was carried out by overnight HRV-3C (Sigma) digestion at 4°C and the cleaved protein was then purified on Ni-NTA before being subjected to SEC on Superose 6 10/300 column (Cytiva ...
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bioRxiv - Biochemistry 2019Quote: ... The His-tag was cleaved by digestion with thrombin (Sigma-Aldrich) in the presence of 2 mM CaCl2 at 37°C for 2 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng recombinant His-AKT1 (inactive, Upstate/Millipore #14-279), at a final volume of 30 μl ...
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bioRxiv - Biochemistry 2020Quote: Recombinant Ded1-His was expressed from the pET22b plasmid (Novagen) and purified as previously described (46) ...
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bioRxiv - Cell Biology 2023Quote: The C19orf43.WT plasmid is comprised of an N-terminal 3x Flag tag followed by human full length C19orf43 cloned into the p3XFlag-CMV-10 vector (Sigma). C19orf43.R7G was obtained by mutating the arginine at position 7 to glycine and C19orf43.R106G was obtained by mutating the arginine at position 106 to glycine ...
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bioRxiv - Neuroscience 2023Quote: The pCMV20-Igκ_SP-FLAG vectors used for cloning were constructed by inserting a human Igκ signal peptide (SP) sequence in front of the FLAG tag of the pCMV20 plasmid (Sigma). Full-length (36-713 ...
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bioRxiv - Immunology 2020Quote: NTD-Fc or RBD-Fc proteins were diluted in sterile PBS and formulated as an emulsion with TiterMax Gold adjuvant (Sigma-Aldrich, St. Louis, MO) to a final antigen concentration of 250 µg/mL ...
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bioRxiv - Immunology 2022Quote: ... For surface staining cells were incubated with mastermix prepared in Fc-block (1%FCS/PBS with 0.1% rat IgG (Sigma-Aldrich, St. Louis, MO, USA)) ...
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bioRxiv - Cell Biology 2021Quote: ... Flag-TMIGD1 constructs in pFlag-CMV-1 (N-terminal Flag tag, Sigma-Aldrich, Munich, Germany): hTMIGD1 without signal peptide (AA 30-262 ...
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bioRxiv - Biophysics 2020Quote: ... FLAG and T7 affinity tags were detected with anti-FLAG (F3165, M2 clone, Sigma-Aldrich) and anti-T7 antibody (69522 ...
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bioRxiv - Developmental Biology 2019Quote: ... supplemented with 10% FCS and 1% penicillin and streptomycin (Sigma). After separation from UGE ...
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bioRxiv - Genetics 2021Quote: ... supplemented with 1 g/ml glucose containing 10% FCS (Sigma), 1% penicillin (10,000 units/ml ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 10% inactivated Fetal Calf Serum (FCS) (Sigma-Aldrich), 100 ng ml−1 L-Glutamine ...
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bioRxiv - Biochemistry 2020Quote: ... the MR-Fc was purified over mannan-agarose beads (Sigma) and protein A-agarose beads ...