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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Recombinant Human Aldehyde Dehydrogenase 1 Family Member A3 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... α-His (Sigma H1029). α-Rabbit IgG HRP conjugate (Promega W4011) ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of purified His fusion protein (PuBZR1-His) was bound to Ni-NTA His binding resin (Novagen). GST fusion proteins containing PuACO1 (PuACO1-GST ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified total RNA (3 μg) was separated by formaldehyde-agarose gel electrophoresis with 1x MOPS buffer and then transferred to Immobilon NY+ member (Millipore) as previously described (Arvola et al ...
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bioRxiv - Microbiology 2019Quote: ... and tagged proteins were detected with mouse anti-GFP (Sigma, 1:5,000), rat anti-HA (Sigma 3F10 clone ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-PAP 1:1000 (for detecting TAP-tagged proteins) (Sigma-Aldrich, P1291), anti-Rad53 1:2000 (abcam ab104232) ...
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bioRxiv - Neuroscience 2024Quote: ... After incubation with peroxidase-tagged secondary antibodies (1:2000 diluted, Sigma-Aldrich), membranes were revealed with an ECL-plus chemiluminescence western blot kit (Amershan-Pharmacia Biotech).
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... followed by HRP-tagged anti-rabbit secondary antibody (1:10,000, Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of recombinant CDK1:CyclinB1 active complex (Millipore) in the presence or absence of 3 mM ATP ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of recombinant CDK1:CyclinB1 active complex (Millipore) in the presence or absence of 3 mM ATP ...
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bioRxiv - Microbiology 2022Quote: ... SasG-His was then re-purified using HIS-Select resin (Sigma) and eluted with bind buffer containing increasing concentrations of imidazole ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with HKSdaFREE and HKGE (AvantBio) and 5 ng/mL human recombinant animal-free KGF (Sigma-Aldrich), 1.64 mM CaCl2 and 50 ug/mL ascorbic acid ...
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bioRxiv - Bioengineering 2019Quote: ... Recombinant human BMP-2 (Pfizer) was reconstituted in a 0.1% solution of rat serum albumin (Sigma-Aldrich) and 4 mM HCl and mixed with alginate ...
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bioRxiv - Systems Biology 2023Quote: ... CD8+ T-cell cultures were supplemented with 120 IU/ml human recombinant IL-2 (Sigma Aldrich, USA). T-cells were aliquoted into five samples with 1.5 x 106 cells in each condition to obtain cells at five different time points (6 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... 400 µl of solution of recombinant TnC (Supplementary Materials and Methods) or purified human TnC (Merck Millipore) (15 µg/ml in 0.01% PBS-Tween ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Neuroscience 2024Quote: The inhibitory activities of 5–7 and all the reference compounds were determined using modified Ellman’s method [25,42,43] against human recombinant AChE (hAChE, E.C. 3.1.1.7, purchased from Sigma-Aldrich, Prague, Czech Republic) and human plasmatic BChE (hBChE ...
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bioRxiv - Cell Biology 2021Quote: ... The gelatin coating was then cross-linked using 0.5% glutaraldehyde and excess aldehyde quenched with sodium borohydride (Sigma) and washed with excess 1X PBS ...
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bioRxiv - Microbiology 2021Quote: ... probed using 1:500 monoclonal anti-His HRP-conjugated antibodies (Sigma) and imaged using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-His antibody (Sigma; 1:5,000 in PBS + 2% BSA) was added and incubated for 1h ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His primary antibodies (dilution 1:3000, #H1029 Sigma Aldrich).
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bioRxiv - Immunology 2022Quote: ... containing 2% (vol/vol) of heat inactivated (HI, for 1h at 56°C) human AB serum (hABS) (Sigma, #H3667). After extensive washings with DPBS (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... glucose-6-phosphate dehydrogenase (loading control; LC) (Sigma-Aldrich, Cat #A9521) (1:50,000) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... saturating amounts of pyruvate kinase and lactate dehydrogenase (Sigma-Aldrich, P0294), and varying amounts of ATP (0.01 to 8 mM) ...
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bioRxiv - Biochemistry 2020Quote: ... and 0.02 units L-glutamate dehydrogenase (Sigma-Aldrich Cat. No. G2626). Reactions were started by adding 2 mM substrate ...
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bioRxiv - Biochemistry 2020Quote: ... and lactate dehydrogenase (LDH) release using the Cytotoxicity Detection KitPLUS (Sigma).
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bioRxiv - Biochemistry 2022Quote: Ammonium concentrations were determined using a glutamate dehydrogenase assay (Sigma-Aldrich) kit using the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... and GAPDH (Glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle, G2267, Sigma) were added above His-PGK attached to the lipid bilayer on the glass bottom Petri dish ...
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bioRxiv - Biochemistry 2019Quote: ... and L-Lactate Dehydrogenase (LDH) from rabbit muscle (Sigma-Aldrich 10127876001). We chose 24 sequences from the citrate synthase assay whose anti-aggregation ability spanned the entire range of our data ...
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bioRxiv - Physiology 2020Quote: ... and 5 mg/ml of G-6P dehydrogenase (Sigma Aldrich, 10165875001)) ...
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bioRxiv - Biochemistry 2020Quote: ... D-Lactic dehydrogenase were purchased from Sigma-Aldrich (Poole, Dorset, UK). Geneticin G-418 (potency rating – 700 µg ...
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bioRxiv - Microbiology 2022Quote: ... and rabbit anti-glucose-6-phosphate dehydrogenase (G6PDH) (#A9521, Sigma-Aldrich) were used as loading controls for F ...
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bioRxiv - Bioengineering 2022Quote: ... and 22.25 units/mL of L-Lactic Dehydrogenase (L3916, Sigma-Aldrich). A standard curve was prepared using 20 mM lactate stock solution by adding sodium L-lactate (L7022 ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... and 24 U L-lactate dehydrogenase from rabbit muscle (Sigma-Aldrich) in buffer containing 150 mM NaCl and 25 mM Tris pH7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... The protocol outlined in the Malate Dehydrogenase Assay Kit by Sigma was followed to measure the renaturation of denatured hMDH using each chaperonin preparation.
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bioRxiv - Microbiology 2024Quote: ... Filters were scanned by densitometry and the relative phosphorylation level of each MAPK member was determined relative to anti α-tubulin antibody T9026 (Sigma) loading control ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% BSA and 1% human serum (Sigma) to block Fc receptors ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CHO cells were stably transfected with a SNAP-tagged human D2SR (short isoform) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 2 mM glutamine (Sigma-Aldrich, Poole, UK) and supplemented with 10% fetal calf serum (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Band intensity was measured as the sum optical density by using ImageJ software (version1.4; NIH) and normalized to control labelling of glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:5000, Millipore).
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bioRxiv - Genetics 2021Quote: ... a mouse anti-glyceraldehyde-3-phosphate dehydrogenase monoclonal antibody (#MAB374, used at 1:10,000 for the Western blot analyses, Merck Millipore); a rabbit anti-LC3B polyclonal antibody (#2775 ...
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bioRxiv - Physiology 2020Quote: ... or mouse anti-S-tag (for S-tagged NTD, 1:1500, Sigma, SAB2702204) and rabbit anti-Nav1.5 (1:150 ...
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bioRxiv - Molecular Biology 2024Quote: ... Tagged proteins were detected by FLAG-HRP conjugated antibody (Sigma, A8592, 1:4000), mouse HA-probe antibody (Thermofisher ...
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bioRxiv - Biochemistry 2020Quote: ... HIS (Sigma-Aldrich H3667), purified full-length Vn (1 μM in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Anti-His (Millipore, USA), anti-GST (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... or anti-His (Novagen) antibodies to detect Ub conjugates.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies at a 1:2000 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-His (Sigma, H1029) 1:2000 ...
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bioRxiv - Plant Biology 2022Quote: ... anti-His (Sigma H1029), anti-GFP (Agrisera ...
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bioRxiv - Bioengineering 2022Quote: ... Epidermal growth factor (EGF) and Recombinant Human Fibroblast Growth Factor Basic (FGF2) Progesterone were purchased from Sigma-Aldrich. OPSS-PEG-SVA (3400 Da) ...