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Citations for Millipore Sigma :
301 - 350 of 3831 citations for Recombinant Human ANGPTL5 GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... or GST (1:1000, Sigma-Aldrich, GE27-4577-01).
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bioRxiv - Molecular Biology 2022Quote: ... followed by Western blotting with anti-GST (Sigma G1160), anti-His (Clontech 631212) ...
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bioRxiv - Microbiology 2019Quote: ... and a rabbit polyclonal anti-GST antibody (Sigma-Aldrich), respectively ...
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bioRxiv - Microbiology 2019Quote: ... The GST tag was cleaved with PreScission Protease (Millipore) and proteins were dialyzed overnight in 50 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... against a primary antibody (GST-tag Monoclonal antibody, Novagen), which is attached to GST-tagged TF proteins (Figure S4).
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bioRxiv - Biophysics 2019Quote: ... GST was cleaved off by biotinylated thrombin (EMD Millipore), and enzyme was removed by streptavidin sepharose resin (EMD Millipore ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: GST fusion proteins were expressed in Rosetta cells (Novagen) at 18°C for 16 hours after induction with 500 μM IPTG at an OD600 of 0.6 ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was removed by thrombin protease (Sigma) in cleavage buffer (20 mM Tris-HCl [pH 8.0] ...
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bioRxiv - Biochemistry 2021Quote: ... GST tag was cleaved by incubating with thrombin (Sigma) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti- GST (Millipore Sigma 16-209, 1:10,000), mouse anti-β-actin (Santa Cruz Biotech sc-47778 HRP ...
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bioRxiv - Microbiology 2022Quote: ... goat polyclonal anti-GST antibody (1:5000, Sigma-Aldrich), mouse monoclonal anti-V5 antibody (1:5000 ...
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bioRxiv - Plant Biology 2023Quote: GST inhibitors and agrochemicals were purchased from Sigma-Aldrich: ethacrynic acid (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Anti-GST antibody (Abcam #ab111947 and Sigma, #G7781-100UL), anti-His (Abcam ...
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bioRxiv - Biochemistry 2021Quote: Genes for His-tagged or Strep II-tagged nanobodies were cloned into the pET 26b vector (Novagen). The expression and purification of all His-tagged nanobodies have been described previously 12 ...
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bioRxiv - Plant Biology 2021Quote: ... The pellet was discarded and supernatant was used to analyze the GST activity (Thounaojam et al. 2012). GST activity was performed according to method (Habig et al. 1974) by utilizing GST Assay kit (Sigma, USA, CS0410-1KT)
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bioRxiv - Cell Biology 2019Quote: ... GST-Mm-RAB8A-Q67L and GST-Rn-RAB27A-Q78L proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore, Novagen, Darmstadt, Germany). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Microbiology 2022Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection and filtered through 0.22μm PES membrane filters (Nalgene) ...
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bioRxiv - Microbiology 2021Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection and filtered through 0.22 μm PES membrane filters (Nalgene) ...
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bioRxiv - Cell Biology 2019Quote: ... S7077) and used at a final concentration of 10 µM. Human recombinant HGF (Cat. Nr. 1404) was purchased from Sigma and used at a final concentration of 100 ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... An identical sample set was prepared at 5µM in a solution containing 35mg/mL recombinant human albumin (#A9731, Sigma-Aldrich) and incubated at 37°C for either 24hr or 48hr with gentle rotation ...
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bioRxiv - Bioengineering 2021Quote: ... cells were cultured for different periods (6, 12 or 24 hours) in different combinations of vascular endothelial growth factor (VEGF, human recombinant; Millipore) and brain-derived neurotrophic factor (BDNF ...
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bioRxiv - Bioengineering 2021Quote: Recombinant human fibroblast activation protein alpha (FAP) expressed in Sf21 cells (400-600 ng/μL) was purchased from Sigma-Aldrich and used at the final concentrations indicated for each experiment ...
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bioRxiv - Bioengineering 2021Quote: ... and 1,500 mg/L sodium bicarbonate (ATCC) supplemented with 2 ng/ml recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (Sigma-Aldrich) and 10 % fetal bovine serum ...
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bioRxiv - Biochemistry 2019Quote: ... STAT1 (0.3 µM) (Biosource, PHF0011) was phosphorylated in vitro by incubation with recombinant human cdk1/cyclinB (0.2 µM) (Millipore, 14-450) at 30 °C for 90 min in the presence of 1 mM ATP ...
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bioRxiv - Bioengineering 2020Quote: ... incubated 30 seconds in plasma cleaner then coated with cell-specific adhesive surface: for at least 1 hour with 10 μg/ml recombinant human fibronectin (Sigma) for Hela and SHSy-5Y cells ...
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bioRxiv - Immunology 2022Quote: ... expanded in the presence of recombinant human IL-2 (1000 U/mL, Proleukin) and rapamycin (50 nmol/L, Sigma-Aldrich), and transduced after 2 days ...
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bioRxiv - Cancer Biology 2022Quote: ... PDAC cells were treated with human recombinant FasL (MiaPaCa2: 50 ng/mL; TKCC10: 100 ng/mL) (Sigma-Aldrich, cat. SRP3036), 48 hours post transfection ...
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bioRxiv - Cell Biology 2022Quote: ... and Mirg-/-littermates injected subcutaneously with Growth factor-reduced Matrigel BD) with 400 ng mL-1 recombinant human bFGF (Millipore). After 7 days Matrigel plugs ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Supplemented IVM media for OSC-IVM consisted of IVM media (Medicult, Origio) supplemented with 75 mIU/ml of recombinant human FSH (Millipore), 100 mIU/ml recombinant human chorionic gonadotropin (hCG ...
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bioRxiv - Neuroscience 2023Quote: ... anti-rabbit TRITC tagged (1:200, Sigma) and anti-mouse FITC tagged (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... or anti-GST primary antibodies (Sigma□Aldrich, Saint Louis, MO) and secondary goat antimouse IgG:IRDye800CW (Li-Cor).
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bioRxiv - Cell Biology 2019Quote: ... Western blot analysis was performed with α-GST (Sigma G7781), α-His (Sigma H1029) ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl of monoclonal mouse-anti-GST antibodies (Sigma-Aldrich), monoclonal anti-His6 antibodies (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: ... GST tag was then cleaved off using preScission protease (Sigma) overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... and analyzed by immunoblotting using either anti-GST antibody (Sigma) or anti-MBP antibody (NEB).
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bioRxiv - Biochemistry 2021Quote: ... Soluble lysates were incubated with GST-Bind resin (EMD Millipore) for 30 min at 4 °C ...
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bioRxiv - Genetics 2020Quote: ... H9 cells were cultured with 6μM Chir99021 (GST inhibitor [Millipore]) in B27-medium (RPMI 1640 medium [Invitrogen] supplemented with 1× B27 minus insulin [50× ...
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bioRxiv - Biochemistry 2022Quote: ... GST proteins were eluted with 10 mM reduced glutathione (Sigma) in 50 mM Tris pH 8.0.
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bioRxiv - Cell Biology 2022Quote: ... Anti-GST-HRP (RPN1236, 1:10000) was from Sigma Aldrich. Anti-phosphotyrosine antibody (4G10 ...
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bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation with anti-GST mAb (1:2000, Sigma) and anti-mouse IgG-HRP (1:2000 ...
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bioRxiv - Biochemistry 2023Quote: ... GST-Erk2 at 20 μg/mL (Sigma-Aldrich, ref. E1283), GST-Cdk1/CyclinA2 at 20 μg/mL (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... GST was removed with the Thrombin CleanCleaveTM Kit (Sigma-Aldrich) following the manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... GST protein was purified using glutathione Sepharose 4B beads (Sigma) by rotating overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The chopped and digested pieces were placed in a serrated 60mm petri dishes and supplemented with 10ng/μl each of human recombinant epidermal growth factor (hEGF, Cat # E9644, Sigma-Aldrich), N2 suppliment-1X (Cat # 17502048 ...
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bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...
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bioRxiv - Genomics 2020Quote: ... RPMI-1640 medium used to culture the MCF7 cells was additionally supplemented with 0.01 mg/ml human recombinant insulin (Sigma Aldrich, #I3536).
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bioRxiv - Cell Biology 2021Quote: ... 250 µl of maintenance medium (50% Wnt3a-conditioned medium (ATCC#CRL-2647, Manassas, VA, USA) and 50% of 2× basal medium supplemented with recombinant human EGF (Sigma-Aldrich), recombinant human noggin (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... the native or reconstituted chromatin samples (140 μg DNA/ml) in HNE were treated with 25 μg/ml human recombinant PAD4 enzyme (Sigma Aldrich cat # SRP0329 or Cayman Chemical cat ...
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bioRxiv - Neuroscience 2023Quote: ... Recombinant Human Protein #PHG0264), 10 ng/ml epithelial growth factor (EGF, Recombinant Human Protein #PHG0311) and 10 µM ROCK Inhibitor Y-27632 (Sigma, #Y0503) were added to the media freshly before use ...