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Citations for Millipore Sigma :
401 - 450 of 3834 citations for Recombinant Human AKT3 GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Bound PEX14-NTD was immunodetected with monoclonal anti-GST antibodies (Sigma-Aldrich) in TBS plus 3% BSA ...
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bioRxiv - Plant Biology 2020Quote: OsDMI3-GST fusion protein was produced in Escherichia coli Rosetta (DE3; Novagen), and tested for interaction as described previously (Ni et al. ...
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bioRxiv - Microbiology 2020Quote: ... GST bound protein was then cleaved using biotinylated thrombin (Merck Millipore; 69672) according to the manufacturers instructions overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... anti-GST HRP Conjugated antibody (Sigma Aldrich; diluted 1:5,000 in PBS) was added to each well (100 μL/well ...
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bioRxiv - Neuroscience 2022Quote: ... the expression of GST fusion proteins was induced with isopropylthiogalactoside (IPTG, Sigma) at 16 °C overnight ...
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bioRxiv - Biophysics 2023Quote: ... GST was cleaved using the Thrombin CleanCleave kit (Sigma-Aldrich cat # RECOMT) for 14 hours at 4 °C with gentle rocking ...
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bioRxiv - Biochemistry 2023Quote: ... GST-Cdk1/CyclinA2 at 20 μg/mL (Sigma-Aldrich, stock ref. C0244) and GST-Cdk2/CyclinA2 at 20 μg/mL (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... and GST-Cdk2/CyclinA2 at 20 μg/mL (Sigma-Aldrich, ref. C0495). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were then washed with buffer and recombinant Tau protein (Sigma recombinant Tau protein #T0576) and ATP (Sigma #A1852 ...
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bioRxiv - Cell Biology 2021Quote: ... peroxidase-anti-peroxidase for detecting TAP-tagged proteins (Sigma); anti-FLAG mouse monoclonal antibody (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... and dextran tagged with fluorescein (40,000 MW, Sigma Aldrich) were resuspended in 100 μl HBSS (4 mg/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged protein was immunoprecipitated using FLAG antibody (Sigma) and Protein G magnetic beads (Invitrogen) ...
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bioRxiv - Genetics 2019Quote: ... FLAG-tagged protein was immunoprecipitated using FLAG antibody (Sigma) and Protein G magnetic beads (Invitrogen) ...
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bioRxiv - Developmental Biology 2019Quote: Biotin-tagged OSTN was labeled by Cy3-streptavidin (Sigma). Equal volume of 25 μM biotin-tagged OSTN and 0.1 mg/ml Cy3-streptavidin were incubated with on ice for 15 minutes at 4 C ...
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bioRxiv - Molecular Biology 2023Quote: ... HTP-tagged proteins were detected using rabbit PAP (Sigma) diluted 10,000 fold ...
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bioRxiv - Biophysics 2023Quote: ... Lot number: SLBT8667), Lysozyme (recombinant, expressed in rice, Lot number: SLCH2681), and Plasma (Human, Lot number: SLBT0202) were purchased from Sigma Aldrich (St. Louis, MO) and received as lyophilized power ...
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bioRxiv - Molecular Biology 2020Quote: ... overexpression of GST-β1a was carried out using Escherichia coli BL21 (DE3) (Novagen) transformed with plasmid containing the cDNA inserted into pGEX-4T-2 vector ...
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bioRxiv - Neuroscience 2021Quote: ... the GST tag was cleaved using the Thrombin CleanCleave Kit (Sigma-Aldrich, SLBZ7194) according to the manufacture’s protocol after elution step ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were then resolved by SDS-PAGE and immunoblotted using GST (Sigma Aldrich), 6His (Sigma Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... as well as with anti GST antibody (Cat no. G7781-2ML, Sigma, USA). GST protein was used as a control for all the pull-down experiments ...
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bioRxiv - Plant Biology 2021Quote: ... GST-PPKs were tested against 5 µg myelin basic protein (MBP; Sigma Aldrich) and 5 µg Casein in 0.1 M Tris pH 8.8 (Sigma) ...
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bioRxiv - Plant Biology 2022Quote: ... 50 µg GST–CAR9 protein emulsified with Incomplete Freund’s adjuvant (F5506, Sigma–Aldrich) was injected into the ICR mouse and then once again in the next week ...
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bioRxiv - Molecular Biology 2019Quote: ... the slide was incubated with anti-GST antibody (Sigma G7781; 1:13,000 dilution) in 5% dried milk in TBS-T for 1hr at 23°C ...
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bioRxiv - Cell Biology 2020Quote: ... actin 224-236-1 (DSHB Iowa University) and GST clone 6G9C6 (Sigma-Aldrich); rabbit polyclonal anti-GFP/EGFP PABG1 (Chromotek) ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing GST-paFur were cleaved using a Thrombin CleanCleave kit (Sigma-Aldrich). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... A GST-Drn fusion protein was produced in Origami B (DE3) cells (Novagen) and purified with a glutathione-Sepharose 4B column ...
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bioRxiv - Cell Biology 2022Quote: Enzymatic cleavage of the GST tag was performed using thrombin (Cat# T1063, Sigma). Supernatants containing cleaved target proteins were incubated with Benzamidine Sepharose 4 flat flow (Cat# 17-5123-10 ...
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bioRxiv - Cell Biology 2022Quote: ... The expressed GST-fusion protein was absorbed to glutathione-Sepharose 4B (Sigma-Aldrich) followed by extensive washing with phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... and the GST tag was removed by cleaving with PreScission Protease (Sigma-Aldrich). The purified proteins were exchanged to the biotinylation buffer containing 25 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... and recombinant chondriotinase ABC (Sigma, C3667) was treated at 25 mU/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... Recombinant active AMPK (Millipore, 14-840) was added and incubated for 30 min at 30°C ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant IFNα was purchased from Sigma (Interferon-αA/D human Cat ...
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bioRxiv - Developmental Biology 2022Quote: ... and Recombinant mouse LIF (Sigma, #ESG1107). Additionally ...
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bioRxiv - Immunology 2021Quote: ... Recombinant purified Angiotensin II (Sigma – A9525) at 1 μM with 2-fold serial dilutions ...
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bioRxiv - Immunology 2022Quote: ... recombinant complement component C3 (Millipore Sigma), recombinant CD4 (Sigma Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... recombinant leukemia inhibitory factor (SRP3316; Sigma), growth hormone (869008 ...
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bioRxiv - Neuroscience 2023Quote: ... and/or recombinant Fibronectin (Sigma-Aldrich) were applied as described using a range of concentrations (μg/ml).
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bioRxiv - Biophysics 2023Quote: ... superoxide dismutase (bovine SOD, recombinant, Sigma) was added from a ≥50,000 U/mL stock prepared in assay buffer ...
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bioRxiv - Cancer Biology 2023Quote: BG4 recombinant antibody (Sigma Aldrich MABE917)- ChIP
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 mIU/ml recombinant hCG (Sigma), and 10 mg/ml of human serum albumin (Sage) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1000U/ml ESGro recombinant LIF (Millipore), 1uM PD0325901 (R&D Systems) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.25x Protease Inhibitor Cocktail for Histidine-tagged proteins (Sigma-Aldrich), and 0.3 mg/mL lysozyme ...
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bioRxiv - Cell Biology 2021Quote: A549 cells expressing GFP-tagged EGFR were obtained from Sigma. Cells were plated on Edge plates (Thermo Scientific ...
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bioRxiv - Genetics 2019Quote: ... The TAP-tagged proteins were probed with PAP (Sigma-Aldrich) (1:2,000 ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG-tagged proteins were immunoprecipitated with αFLAG (clone M2, Sigma) beads (Wang et al ...
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bioRxiv - Microbiology 2023Quote: N-terminal His-tagged KtrD cloned into pRSFDuet-1 (Novagen) was overexpressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Biochemistry 2023Quote: ... 25 uL of (His)6-tagged TEV-protease (Sigma-Aldrich) was added and the solution incubated overnight at 4°C.
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bioRxiv - Biochemistry 2023Quote: ... 10 µl protease inhibitor cocktail for histidine tagged proteins (Sigma) / 0.2 g weight of E ...
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bioRxiv - Biochemistry 2022Quote: pCold-GST GTSF-expression vectors were transformed into Rosetta-Gami 2 competent cells (Novagen). Cells were grown in the presence of 1 μM ZnSO4 at 37°C until OD600 ∼0.6–0.8 ...
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bioRxiv - Cell Biology 2022Quote: ... and the GST tag was cleaved by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4 °C ...