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Citations for Millipore Sigma :
601 - 650 of 4637 citations for Recombinant Human ACVR1 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: A549 cells expressing GFP-tagged EGFR were obtained from Sigma. Cells were plated on Edge plates (Thermo Scientific ...
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bioRxiv - Genetics 2019Quote: ... The TAP-tagged proteins were probed with PAP (Sigma-Aldrich) (1:2,000 ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG-tagged proteins were immunoprecipitated with αFLAG (clone M2, Sigma) beads (Wang et al ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µl protease inhibitor cocktail for histidine tagged proteins (Sigma) / 0.2 g weight of E ...
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bioRxiv - Plant Biology 2023Quote: ... GST-tagged protein fractions were incubated with thrombin (Sigma, Germany) at 4°C overnight for cleavage of the GST tag (Step 2 ...
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bioRxiv - Bioengineering 2023Quote: ... Either 15 μg of nanobody Fc plasmid or 1.5 μg of nanobody Fc plasmid and 13.5 μg of ssDNA (Sigma, D7656) were combined with 45 μg PEI (Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Biochemistry 2022Quote: ... Recombinant plasmids were transformed into TUNER (Novagen) cells in LB broth containing 10 μg/ml kanamycin at 37 °C shaking at 180 rpm ...
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bioRxiv - Immunology 2022Quote: ... DNAse I recombinant (Roche by Sigma Aldrich) and sodium pyruvate (Sigma Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 25 μg/l recombinant mouse (Sigma-Aldrich) or human epithelial growth factor (EGF ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant 6His-ErbB3 was purchased from Sigma. The in vitro binding assay was conducted by incubating 1 μg/ml recombinant ErbB3 with 1 μg/ml recombinant Rabaptin5 in a stringent buffer containing 50 mM Tris ...
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bioRxiv - Developmental Biology 2022Quote: ... 103 IU recombinant mouse LIF (Millipore, ESG1107), 2mM L-glutamine (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... a recombinant HRV 3C protease (Sigma-Aldrich) was added to NSP7/NSP8 complex and incubated overnight at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.8μg of recombinant LSD1 (Sigma-Aldrich SRP0122) was used as positive control ...
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bioRxiv - Cell Biology 2024Quote: ... and recombinant PRMT5/MEP50 (Sigma-Aldrich, #SRP0146) was mixed with 0.5 µg in a buffer solution ...
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bioRxiv - Neuroscience 2022Quote: ... wells were washed three times using H2O 0.05% Tween20 and binding of NMDAR antibodies to the immobilized N1-N2B-Fc was detected by using goat-anti-hIgG(Fc)-HRP (1:70000, A0170, Sigma). Binding was visualized by using TMB substrate ...
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bioRxiv - Molecular Biology 2021Quote: ... AID-tagged proteins were conditionally depleted using 250mM auxin (Sigma, I2886) stock dissolved in ethanol at a final concentration of 0.5mM ...
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bioRxiv - Cell Biology 2021Quote: ... The tagged proteins were immunoprecipitated using FLAG M2 antibody beads (Sigma). Co-precipitating RNAs were reverse transcribed using random hexamers and Superscript III (Life Technologies) ...
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bioRxiv - Systems Biology 2019Quote: ... and un-tagged CARD14 was detected with anti-CARD14 (HPA023388, Sigma).
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bioRxiv - Molecular Biology 2020Quote: ... and α-FLAG (detecting C-terminal 3x FLAG-tagged constructs, Sigma). Blots were probed using HRP-conjugated Secondary Antibody (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged proteins were purified using FLAG M2 affinity gel (Sigma) rotated end over end for 3 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... HA-tagged proteins were immunoprecipitated with anti-HA agarose beads (Sigma) and processed for Western blotting as previously described.
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bioRxiv - Microbiology 2023Quote: ... a C-terminal 6His-tagged version was cloned onto pET22b (Novagen) for expression in E ...
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bioRxiv - Immunology 2024Quote: ... The normalized supernatants were mixed with FLAG-tagged beads (Sigma, A2220) at 4°C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD5 was detected using anti-FLAG mouse antibody (Sigma) and cMyc using anti-c-Myc rabbit antibody clone 7E18 (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with anti-FLAG M2-agarose (Sigma), following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... and 10% Fetal Calf Serum (FCS, Sigma-Aldrich). The CEM T cell line was provided by the ATCC ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 10% fetal calf serum (FCS) (Sigma), 100 U/ml penicillin/streptomycin (Gibco) ...
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bioRxiv - Developmental Biology 2021Quote: ... and 10% Fetal Calf Serum (FCS, F2442, Sigma) and 1000 U/ml LIF on plates coated with 0.1 % gelatin ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 5% heat inactivated FCS (Sigma Aldrich cat. # 12133C) and Penicillin-Streptomycin (100 units/mL) ...
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bioRxiv - Cell Biology 2019Quote: ... We injected Fluorinert FC-70 (Sigma, Ref. F9880) with a density of 1.9 times the density of water at the maximal pressure of the microinjector (Clean mode at 7000 hPa) ...
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bioRxiv - Genomics 2020Quote: ... 15% v/v FCS (Sigma F7524, lot: 091M3398), 1% v/v penicillin-streptomycin (PAN Biotech P06-07100) ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% (v/v) FCS (Sigma-Aldrich) and penicillin/streptomycin (Gibco ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... 10%FCS (all reagents were from Sigma-Aldrich). Floating primary testicular germ cells were collected and cultured onto laminin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 2% Fetal Calf Serum (FCS (Sigma) or STEMCELL Technologies (SCT)) ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% (vol/vol) FCS (Sigma-Aldrich) and penicillin/streptomycin (Gibco ...
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Cytokine and phenotypic cell profiles in human cutaneous leishmaniasis caused by Leishmania donovanibioRxiv - Microbiology 2022Quote: ... USA),10% of fetal calf serum (FCS)(Sigma, USA) and100 U/mL Penicillin-Streptomycin(Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 5-fluorouracil (5-FC, Sigma-Aldrich, cat#F6627) was prepared in drinking water at a final concentration of 2.0 mg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% (v/v) FCS (Sigma-Aldrich) and penicillin/streptomycin (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma), penicillin (100 U/ml ...
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bioRxiv - Immunology 2020Quote: ... and supplemented with 10% FCS (Gibco) (Sigma-Aldrich), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2020Quote: ... and 10% Fetal Calf Serum (FCS, F2442, Sigma) and 1000 U/ml LIF ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10% FCS (Sigma-Aldrich, Dorset, UK), 1 mM sodium pyruvate and 1% non-essential amino acids (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... containing 10% foetal calf serum (FCS; Sigma Aldrich), until staining.
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bioRxiv - Microbiology 2022Quote: ... and 10% fetal calf serum (FCS; Sigma Aldrich). Both cell lines were regularly monitored for mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza Rockland) ...