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Citations for Millipore Sigma :
251 - 300 of 3831 citations for Recombinant Human ACP1 GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... 1:10000 for anti-GST (SIGMA, USA), 1:5000 for anti-HA (SIGMA ...
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bioRxiv - Biochemistry 2021Quote: ... in place of the GST gene (Novagen).
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bioRxiv - Cell Biology 2019Quote: ... and GST (1:10,000, Sigma A-7340). Appropriate horseradish peroxidase-labeled secondary antibodies (Jackson Immunoresearch ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-GST antibody (Sigma Aldrich, 06-332) was added diluted 1:1,000 in PBS-T with 3% BSA and incubated with gentle agitation in RT for 1 hr ...
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bioRxiv - Plant Biology 2023Quote: ... 1:5,000 mouse anti-GST (Sigma SAB4200237); 1:2,000 rabbit anti-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Biochemistry 2024Quote: ... a primary anti-GST (Sigma, 1:3,000) was used to detect bound PI(4,5)P2-grip protein.
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bioRxiv - Bioengineering 2022Quote: ... supplemented with HKSdaFREE and HKGE (AvantBio) and 5 ng/mL human recombinant animal-free KGF (Sigma-Aldrich), 1.64 mM CaCl2 and 50 ug/mL ascorbic acid ...
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bioRxiv - Bioengineering 2019Quote: ... Recombinant human BMP-2 (Pfizer) was reconstituted in a 0.1% solution of rat serum albumin (Sigma-Aldrich) and 4 mM HCl and mixed with alginate ...
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bioRxiv - Systems Biology 2023Quote: ... CD8+ T-cell cultures were supplemented with 120 IU/ml human recombinant IL-2 (Sigma Aldrich, USA). T-cells were aliquoted into five samples with 1.5 x 106 cells in each condition to obtain cells at five different time points (6 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... 400 µl of solution of recombinant TnC (Supplementary Materials and Methods) or purified human TnC (Merck Millipore) (15 µg/ml in 0.01% PBS-Tween ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Neuroscience 2024Quote: The inhibitory activities of 5–7 and all the reference compounds were determined using modified Ellman’s method [25,42,43] against human recombinant AChE (hAChE, E.C. 3.1.1.7, purchased from Sigma-Aldrich, Prague, Czech Republic) and human plasmatic BChE (hBChE ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted fractions from each pull down (Ni-NTA and GST-affinity) were probed with anti-GST (1:3000) antibody (Sigma) and anti-His (1:3000 ...
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bioRxiv - Cell Biology 2022Quote: 0.5 μM RABL3 and 0.05 μM GST or GST-RAB11 were mixed with Glutathione-Sepharose 4B in the presence of 10 μM GTPγS (Sigma Aldrich) or 10 μM GDP (Sigma Aldrich ...
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bioRxiv - Plant Biology 2021Quote: The coding sequence of Hd3a and RFT1 were cloned into the pCold-GST vector42 and produced as a glutathione S-transferase (GST) fusion protein in Escherichia coli BL21 Rosetta (DE3) (Novagen). Cells were grown in LB medium or minimal medium containing 0.5 g l™1 of 15N-ammonium chloride ...
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bioRxiv - Cell Biology 2021Quote: ... polyclonal anti-GST rabbit IgG (Millipore-Sigma, G7781), StarBright® B700 labeled goat anti-mouse IgG secondary antibody (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... polyclonal anti-GST rabbit IgG (Millipore-Sigma, G7781), StarBright® B700 labeled goat anti-mouse IgG secondary antibody (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-GST tag (G1160, Sigma 1:2000), mouse anti-His tag (HIS.H8 / EH158 ...
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bioRxiv - Microbiology 2020Quote: ... The GST tag was removed by thrombin (Millipore) cleavage and the cleaved product exchanged into NMR buffer (50 mM Na phosphate at pH 6.7 ...
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bioRxiv - Biochemistry 2021Quote: ... GST (G7781) and Flag (F3165) from Sigma-Aldrich.
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bioRxiv - Neuroscience 2021Quote: ... and rabbit anti-GST (Sigma G7781, 1:3,000).
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bioRxiv - Plant Biology 2022Quote: ... detected via anti-GST antibody (Sigma Aldrich, A7340), served as a positive control (not shown).
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bioRxiv - Pathology 2023Quote: ... and α-GST (1:10, 000, Sigma-Aldrich). Secondary α-rabbit ...
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bioRxiv - Genetics 2023Quote: ... Primary rabbit anti-GST polyclonal antibody (Sigma, G7781) (1:160,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1:4000 rabbit anti-GST antibody (G7781, Sigma) and mouse anti-His (1:2500 ...
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bioRxiv - Cell Biology 2023Quote: ... or anti-GST antibodies (Sigma-Aldrich, Darmstadt, Germany).
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bioRxiv - Cell Biology 2023Quote: ... followed by anti-GST (1:500, Sigma, SAB4200692) and secondary staining anti-Mouse-DyLight405 (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... anti-glutathione-S-transferase (GST) (G7781; Sigma-Aldrich), anti-InlC (15) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CHO cells were stably transfected with a SNAP-tagged human D2SR (short isoform) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 2 mM glutamine (Sigma-Aldrich, Poole, UK) and supplemented with 10% fetal calf serum (Life Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... Epidermal growth factor (EGF) and Recombinant Human Fibroblast Growth Factor Basic (FGF2) Progesterone were purchased from Sigma-Aldrich. OPSS-PEG-SVA (3400 Da) ...
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bioRxiv - Immunology 2020Quote: ... Transduced CD8+ T cells were expanded in the presence of 10 U/ml recombinant human IL-2 (Sigma) Human CD8+ T cells were purified from donor PBMCs (Cambridge Bioscience or NHSBT ...
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bioRxiv - Cell Biology 2020Quote: ... the cell medium was replaced with DMEM containing 0.2% FBS and 300ng/mL IGF-1 (human recombinant, Sigma) for 3 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.1 IU/mL insulin and 10 ng/mL human recombinant epidermal growth factor (EGF) (all from Sigma-Aldrich). TKCC10 cells were grown in a 1:1 mixture of M199 medium and Ham’s F12 medium ...
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bioRxiv - Biochemistry 2023Quote: ... cells were incubated with medium containing 10 µg/mL recombinant human adiponectin (SRP4901, Sigma-Aldrich, in sterile water), a quantity equivalent to typical adiponectin levels in lean women (Ding et al. ...
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bioRxiv - Immunology 2023Quote: RVT1 association with the recombinant form of human PF4 and UPS-grade protamine (Millipore-Sigma, St. Louis, MO) were characterized using a Synapt G2 HDMS (Waters Corp. ...
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bioRxiv - Immunology 2023Quote: RVT1 association with the recombinant form of human PF4 and UPS-grade protamine (Millipore-Sigma, St. Louis, MO) were characterized using a Synapt G2 HDMS (Waters Corp. ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were expanded in the presence of 10 U/mL recombinant human IL-2 (11147528001, Sigma). Human CD8+ T cells were purified from donor PBMCs (NHSBT or Karolinska Hospital ...
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bioRxiv - Bioengineering 2020Quote: ... The eluted His-tagged RBD was then incubated with biotin-tagged HRV C3 protease (Sigma) and passed through a streptavidin-agarose column to deplete the protease and the 8XHis-SBP peptide ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant MEK and recombinant ERK were purchased from Sigma. Indicated amounts of recombinant GST-HSF-1 (0.1–0.4 mg ...
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bioRxiv - Cell Biology 2022Quote: ... and GST proteins were eluted in GST elution buffer (50 mM Tris-HCl pH 8, 10 mM glutathione (Sigma-Aldrich; G4251). The supernatant was separated from the resin to a fresh tube ...
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bioRxiv - Biochemistry 2024Quote: ... PLA2G15 used in the reactions was obtained by overexpressing C-terminally FLAG-tagged human PLA2G15 in HEK293FT cells and affinity-isolation using anti-FLAG magnetic beads (Sigma-Aldrich #M8823).
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bioRxiv - Biochemistry 2021Quote: ... and 5% glycerol) and eluted in GST elution buffer (GST wash buffer supplemented with 20 mM Reduced Glutathione (Sigma; St. Louis, MO)) ...
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bioRxiv - Genomics 2022Quote: ... FITC-tagged NeuN antibody (Sigma, MAB377) was prepared following manufacturer’s instructions (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich). After 24 hours of CHIR99021 stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... We dissected ovaries in live imaging media (Schneider’s Drosophila medium containing 15% fetal bovine serum (FBS) and 200 mg/mL recombinant human insulin (Sigma)) ...
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bioRxiv - Microbiology 2022Quote: ... difficile spores were incubated with 10 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) with PBS for 1 h at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Mouse primary cortical cultures were then treated with 0.5 μg/ml human recombinant tissue inhibitor of metalloproteinase-2 (TIMP2, Sigma) for one biological replicate or 10 μM BB94 for two biological replicates ...
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bioRxiv - Developmental Biology 2024Quote: ... as controls and analyzed for promoter activation in the presence of 3mg/l recombinant human SPARC (SRP3159, Sigma-Aldrich) using the Dual-Luciferase® Reporter Assay System (E1910 ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: Recombinant human MMP-2 (R&D) and MMP-9 (R&D) were activated using p-aminophenylmercuric acetate (APMA, Sigma) in MMP assay buffer (50 mM Tris (Fisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... The GST beads were washed with lysis buffer (Millipore) three times for 5 min each time ...