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Citations for Millipore Sigma :
251 - 300 of 9774 citations for Recombinant H9N2 A Guinea fowl Hong Kong WF10 99 HA Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... GFP-tagged SAMHD1 proteins were immunoprecipitated with anti-SAMHD1 antibody (Sigma-Aldrich, SAB1400478) bound to Protein-A/G-Agarose (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... Flag-tagged proteins were bound to anti-FLAG M2 affinity gel (Sigma-Aldrich) for 1 h at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... FLAG-tagged proteins were purified using anti-FLAG M2 Affinity agarose gel (Sigma) for >1h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected using anti-FLAG M2 primary (Sigma-Aldrich, F1804) antibodies combined with anti-mouse-HRP secondary (Rockland ...
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bioRxiv - Molecular Biology 2024Quote: ... Tagged proteins were detected by FLAG-HRP conjugated antibody (Sigma, A8592, 1:4000), mouse HA-probe antibody (Thermofisher ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged protein was isolated with anti-FLAG M2 magnetic beads (Sigma, M8823) for 2 hr at 4LJ°C and eluted with 100 μg/ml 3x FLAG peptide ...
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bioRxiv - Microbiology 2019Quote: ... PTP-tagged proteins were detected by incubating with anti-protein A serum (1:3000, 1 h) (Sigma, P3775) followed by Alexa Fluor® 594 goat anti-rabbit (1:250 ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified over HIS-Select (Sigma–Aldrich, St. Louis, MO) nickel affinity resin in 20 mM Tris (pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... Bound protein was detected using anti-His-HRP conjugate (Sigma; 1:10,000). All proteins and antibodies were in 0.1 M phosphate buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tag of the eluted protein was cleaved by thrombin (Sigma), incubated at 4 °C overnight ...
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bioRxiv - Genetics 2023Quote: ... Protein expression was verified and quantified by Western blot using recombinant GST protein (Sigma, G5663) as expression standards ...
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bioRxiv - Plant Biology 2024Quote: ... total proteins were detected with anti–HA–peroxidase (Sigma, clone 3F10), anti–FLAG (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... 3,4-dimethylphenol (DMP) (99% Sigma-Aldrich), 2,4-DMP (98% Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2020Quote: ... Glucose (50-99-7, Sigma-Aldrich), DMEM High glucose (4.5g/L ...
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bioRxiv - Bioengineering 2021Quote: ... Monomers N-phenylacrylamide (PHE; Sigma, 99%) and N-isopropylacrylamide (NIPAM ...
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bioRxiv - Bioengineering 2021Quote: ... ether (Sigma, Certified ACS, Anhydrous,>99%) and CDCl3 (Acros ...
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bioRxiv - Bioengineering 2021Quote: ... and N-isopropylacrylamide (NIPAM; Sigma, >99%) were used as received ...
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bioRxiv - Microbiology 2020Quote: Silver nitrate (AgNO3, Sigma-Aldrich, ≥99%), sodium citrate (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... N-isopropylacrylamide (NIP, >99%; Sigma-Aldrich) was used as received ...
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bioRxiv - Cancer Biology 2020Quote: ... 99% enriched deuterium oxide (Sigma, 151882). All cells were cultured at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... acetic anhydride (>99%) from Sigma Aldrich; HCTU (reagent grade ...
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bioRxiv - Bioengineering 2022Quote: ... and α-methylbenzylamine (99%, Sigma Aldrich)) in N-methyl-2-pyrrolidone (NMP ...
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bioRxiv - Microbiology 2022Quote: ... lactate (99 atom % 13C; Sigma Isotec), oxalate (99 atom % 13C ...
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bioRxiv - Microbiology 2022Quote: ... oxalate (99 atom % 13C; Sigma Isotec), palmitic acid (99 atom % 13C ...
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bioRxiv - Microbiology 2023Quote: ... trifluoroacetic acid (TFA) (Sigma-Aldrich, 99%), chloroform (Merck) ...
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bioRxiv - Plant Biology 2023Quote: ... phlorizdin (≥99%) were purchased from Sigma–Aldrich (St Louis ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... fresh 2-mercaptoethanol (99%, Sigma– Aldrich), and 0.1% sodium dodecyl sulfate (SDS ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes probed for p62 were washed with 0.01% TBS-Triton X and incubated in biotinylated anti-guinea pig (goat polyclonal, EMD Millipore, cat #AP193B, 1:2500) secondary antibody ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant SIRV2 gp21 and SiL_0190 with C-terminal His-tag were expressed using Escherichia coli RosettaTM (DE3) cells (Novagen). Protein expression was induced during logarithmic growth through the addition of 0.5mM isopropyl-thiogalactopyranoside (IPTG ...
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bioRxiv - Plant Biology 2021Quote: ... the total protein was extracted from tobacco expressing CmTPL1-2-HA and CmSVP-FLAG using anti-HA affinity gel (E6779, Sigma), followed by western blotting with anti-FlAG (A8592 ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant proteins were induced in Rosetta™ (DE3) Competent Cells (Novagen) with 0.02% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Cell Biology 2020Quote: ... To induce recombinant proteins isopropyl-β-D-thiogalactoside (IPTG, Sigma-Aldrich) was added (0.1 mM ...
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bioRxiv - Cell Biology 2021Quote: Recombinant protein expression vectors were constructed from the pET28a series (Novagen) encoding an N-terminal hexa-histidine tag and thrombin cleavage site.
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bioRxiv - Biophysics 2022Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Developmental Biology 2023Quote: ... 103 units/mL ESGRO recombinant mouse LIF protein (Millipore Sigma, ESG1107), 2 μM PD0325901 (Tocris ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant fusion protein was purified with Glutathione Sepharose beads (Sigma). Definite probes were designed for different cis-elements and their mutated versions (Table S3) ...
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bioRxiv - Developmental Biology 2024Quote: ... Recombinant protein was produced in Escherichia coli strain BL21(DE3) (Novagen). The cells were grown in LB medium at 37°C and the protein expression was induced at OD600 of 0.7 with 0.2 mM IPTG (Isopropyl β-d-1-thiogalactopyranoside ...
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bioRxiv - Neuroscience 2022Quote: ... and ≥99% 4-methyl-3-heptanol and 99% 6-methyl-5-hepten-2-one from Sigma-Aldrich (Item numbers M48309 and M48805-100ML ...
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bioRxiv - Microbiology 2023Quote: ... pET28a-His-DNTTIP1 has been generated by in frame insertion of DNTTIP1 cDNA into BamH1/Xho1 sites of pET28a(+) (Novagen, kindly provided by Dr ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged ANP32 proteins were detected using mouse anti-FLAG M2 primary antibody (Sigma), followed by goat anti-mouse Alexa Fluor 568 (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... and Flag-tagged FtsX proteins were detected by using ANTI-FLAG® antibody (Sigma) as previously described by Stamsås et al ...
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TIR signaling promotes the interactions between EDS1/PAD4 and ADR1-L1 and oligomerization of ADR1-L1bioRxiv - Plant Biology 2021Quote: ... The FLAG-tagged proteins were immunoprecipitated using 20 μl M2 beads (Sigma Cat. #A2220) and biotinylation was detected by Streptavidin-HRP (Abcam Cat ...
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bioRxiv - Microbiology 2023Quote: ... SPA-tagged proteins were detected with monoclonal anti-Flag M2 antibody purchased from Sigma. YbgF protein was used as an internal control and revealed with polyclonal anti-YbgF antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... Flag-tagged protein was purified using 20µl anti-Flag® M2 magnetic beads (Sigma) per plate ...
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bioRxiv - Biophysics 2023Quote: ... GST-tagged Elf1 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Glutathione Sepharose 4 Fast Flow resin (GE Healthcare) ...
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bioRxiv - Systems Biology 2024Quote: ... Tagged proteins were detected using mouse α-Flag M2 (Sigma Aldrich F3165, 1:10,000), mouse α-V5-tag (Thermo Fisher R960-25 ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected with an anti-FLAG antibody (1:500, Sigma F1804). Samples were also stained with DAPI (300 nM ...
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bioRxiv - Cell Biology 2024Quote: ... respectively (Millipore Cat# HI-14K, HI-13K). Samples treated with 3 mM glucose KRBH were measured undiluted ...
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bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...