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Citations for Millipore Sigma :
1 - 50 of 1696 citations for Recombinant Cynomolgus MET Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged and non-Fc-tagged proteins were concentrated using Amicon Ultra 10 kDa MWCO centrifugal filter units (Millipore), dialyzed against PBS ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was eluded with 250 mM imidazole (Sigma).
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bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: His-tagged recombinant proteins were expressed in Rosetta2 (DE3)pLysS bacteria (Novagen) and purified on IMAC columns (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The MET was quantified based on the external calibration using the MET (Sigma, PHR1084) as standard and its recovery ranged from 98% to 126% with quantification based on external calibration and recovery ...
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Therapeutic effects of Chlorzoxazone, a BKCa channel agonist, in a mouse model of Fragile X syndromebioRxiv - Neuroscience 2020Quote: ... MET (Metformin, Sigma Aldrich, France) and GAB (Gaboxadol Hydrochloride ...
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bioRxiv - Biochemistry 2020Quote: ... Metformin (Met, PHR1084, Sigma-Aldrich), Geraniin (GN ...
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bioRxiv - Developmental Biology 2022Quote: ... cynomolgus and porcine fibroblasts were cultured in high-glucose DMEM (Sigma) supplemented with 15% FBS ...
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bioRxiv - Cell Biology 2022Quote: The recombinant GST-tagged Nanobody against GFP69 expressed in Escherichia coli Rosettagami (Novagen, 71351) was purified with Glutathione Sepharose 4B beads according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... or 1 mM Met (Sigma-Aldrich). Different amounts of Met (0 ...
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bioRxiv - Developmental Biology 2021Quote: ... We injected in each matrix 25 μg of Recombinant Sema3C-Fc (Sigma-Aldrich) in a concentration of 50 μg/ml in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 mM Met (PHR1084, all Sigma Aldrich). Redosing was performed after 3 days ...
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bioRxiv - Evolutionary Biology 2020Quote: ... + CSM -Met (Formedium) + 2% dextrose (Sigma-Aldrich), diluted to an OD600 of 0.1 and measured after 15h.
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bioRxiv - Systems Biology 2020Quote: ... + CSM -Met (Formedium) + 2% dextrose (Sigma-Aldrich), diluted to an OD600 of 0.1 and measured after 15h.
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bioRxiv - Evolutionary Biology 2023Quote: ... L-methionine (L-met, Sigma-Aldrich #M22060), L-aspartic acid (L-asp ...
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bioRxiv - Biochemistry 2020Quote: ... for mFc tagged antigen versions or goat-anti-hIgG(Fc) conjugated with HRP (1:70000, A0170, Sigma) if hFc-tagged antigens had to be detected ...
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bioRxiv - Molecular Biology 2022Quote: ... and 25mL cultures were inoculated at a starting OD600 of 0.1 in SD-Met+Glu or SD-Met+Glu supplemented with 0.048M Ferric (Fe)-EDTA (Ethylenediaminetetraacetic acid ferric sodium salt, E6067, Millipore Sigma) in 125mL Erlenmeyer flasks (optimal Fe-EDTA dosage was determined by a titration experiment (Figure S11)) ...
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bioRxiv - Immunology 2020Quote: ... 48 h and 72 h following treatment cells were pulse-labelled with 500 Ci/mmol 35S-Methionine (35S-Met; MP Biomedical) in Met-free media (Sigma) for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: ... 201.3 μM Met (63-68-3, Sigma-Aldrich) and 4 mM glutamine (Gln ...
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bioRxiv - Biophysics 2022Quote: ... AVP and met-enkephalin were purchased from Sigma. The following plasmids were already described ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-MET (EMD Millipore 07-283, Abcam ab51067), anti-MYC (Abcam ab32072) ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: Recombinant S-tagged TopoII-CTD proteins were loaded on S-protein Agarose beads (#69704, EMD Millipore/ Novagen) by incubating them together overnight ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: Recombinant S-tagged TopoII-CTD proteins were loaded on S-protein Agarose beads (#69704, EMD Millipore/ Novagen) by incubating them together overnight ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged recombinant substrates were purified by immunoaffinity chromatography using the anti-FLAG M2-agarose beads (Sigma), according to the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2021Quote: ... with the control and labeling diets containing regular L-methionine (Met) and methyl-13C-L-methionine (13C-Met, 99%, Sigma-Aldrich), respectively (i.e. ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant FLAG-tagged zebrafish Grk1a and Grk1b were each inserted into the pFLAG-CMV2 vector (#E7033, Millipore-Sigma). HEK-293 cells cultured in DME/F-12 with 10% fetal bovine serum were transfected with either of these constructs using FuGENE 6 Transfection Reagent (#E2691 ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant FLAG-tagged zebrafish Grk1a and Grk1b were each inserted into the pFLAG-CMV2 vector (#E7033, Millipore-Sigma). HEK-293 cells cultured in DME/F-12 with 10% fetal bovine serum were transfected with either of these constructs using FuGENE 6 Transfection Reagent (#E2691 ...
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bioRxiv - Cell Biology 2023Quote: 2 μg GST-tagged NuSAP point mutants were incubated with 50 ng human recombinant Aurora A (Sigma-Aldrich) in a water bath at 30°C for 30 min in kinase buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... 5’-dAH and Met (Millipore Sigma, St. Louis, MO) were added to the resulting solution to final concentrations of 2 mM each ...
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bioRxiv - Immunology 2022Quote: ... N-Formyl-Met-Leu-Phe (fMLP, Sigma-Aldrich; 47729) was dissolved in DMSO (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged recombinant proteins were detected using an HRP-conjugated anti-His monoclonal antibody (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... centrifuged 20 min at 20000 g and recombinant GST-tagged proteins were purified using the GST-BindTM Kit (Novagen). To synthetize the NOVA2 RNA target ...
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bioRxiv - Physiology 2020Quote: ... in combination with Metoclopramide (MET) (product code M0763, Sigma, Spain), a dopamine antagonist ...
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bioRxiv - Microbiology 2022Quote: ... THY added with 10 μM human met-hemoglobin (Sigma-Aldrich), THY added with 5 μM heme (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... formyl-Met-Leu-Phe (fMLP) from Sigma (St. Louis, MO), Nordihydrogualaretic acid (Cayman Chemical ...
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bioRxiv - Physiology 2023Quote: ... Supernatants from cells overexpressing Fc-tagged FGL1 proteins were collected after 5 days and supplemented with protease inhibitor cocktail (Sigma). Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare ...
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bioRxiv - Cell Biology 2021Quote: For conjugation of SNAP-tagged proteins (paxillin, kindlin) recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼50 μM ...
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bioRxiv - Microbiology 2020Quote: Female BALB/c mice have been injected intraperitoneally with 700 μg recombinant His-tagged TGT protein that was emulsified in complete Freund’s adjuvant (Sigma-Aldrich). Two weeks later ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged recombinant protein was eluted by three sequential incubations of GSH-beads with 50mM reduced L-GSH (SIGMA, G4251) in 100mM Tris-HCl [pH8.0],150mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... treatments were initiated by administering MET and INH (both from Sigma) alone or in combination (MET+INH ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: An 8% stock solution of the compound methoxyfenozide (MET) (Sigma-Aldrich) was prepared in DMSO ...
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bioRxiv - Biophysics 2021Quote: ... with 1 µM N-Formyl-Met-Leu-Phe (fMLP, Sigma Aldrich), and then were then plated in a 3D-printed electrotaxis chamber composed of polylactic acid (38) ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cells and large quantities of His6 tagged recombinant Nbs were purified with 1ml of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) following periplasmic expression through osmotic shock ...
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bioRxiv - Immunology 2023Quote: ... The pull-downed proteins were separated by SDS-PAGE on 10-to-20% gradient gel (ATTO) and the recombinant FLAG- tagged WT sCTLA-4 was detected by anti-FLAG M2 antibody (Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
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bioRxiv - Immunology 2023Quote: N-Formyl-Met-Leu-Phe [FMLP] (Sigma-Aldrich; Saint Louis, MO, USA) was added to the top of the Matrigel to induce a chemokine gradient ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse recombinant EphrinB1-Fc chimera (cat # E0653-200UG) and bovine serum albumin (cat #05470-1G) with purity >98 % were from Sigma. Alexa Fuor-594 BSA (cat #A13101) ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... For capture of antibodies and Fc-Fusions a CM5-S-Series sensor chip (Cytiva) was functionalized with 2500RU recombinant Protein A (Sigma) using EDC/NHS Coupling Kit (Biacore/Cytiva BR-1000-50 ...