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Citations for Millipore Sigma :
701 - 750 of 1654 citations for Real Time Thermal Cycler Thermocycler Accessories since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... in log-phase growth (OD = 0.5) was concentrated 10x times (OD = 5) by a glass filter system with a 0.45 μm filter (Millipore). For osmolyte step experiments ...
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bioRxiv - Molecular Biology 2019Quote: ... The plates were washed 3 times with pre-cooled PBS prior to the addition of L-DOPA (Sigma). After incubation in L-DOPA for 1 day ...
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bioRxiv - Immunology 2020Quote: ... coated plates were washed one time with 100 μl/well PBS with 0.05% Tween 20 (PBST) (Sigma-Aldrich), blocked with 100 μl/well of 1% human serum albumin (HAS ...
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bioRxiv - Cell Biology 2019Quote: ... Immune complexes were washed two times with lysis buffer and eluted with 0.5 mg/mL Flag peptide (Sigma) in lysis buffer ...
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bioRxiv - Bioengineering 2020Quote: ... Fixed collagen samples were washed 3 times with a solution containing 50 mM PIPES pH 7.4 (Sigma, P6757) kept in ice and then they incubated for 2h in ice and in the dark in a freshly prepared staining solution (SSI ...
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bioRxiv - Cancer Biology 2019Quote: ... washed 3 times with phosphate-buffered saline (PBS) and permeabilized in 0.4% Triton X-100 (T878, Sigma-Aldrich), 1% donkey serum (D9663 ...
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bioRxiv - Immunology 2019Quote: ... Sections were then washed for three times with wash buffer before mounting in Fluoromount Aqueous Mounting Medium (Sigma). WGA-488 and tdTomato were visualized directly based on endogenous fluorescence ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... 0.1% SDS), two times with LiCl wash buffer (10mM Tris-HCl pH 8.0, 1mM EDTA, 250mM LiCl (Sigma), 0.5% NaDeoxycholate ...
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bioRxiv - Systems Biology 2019Quote: Yeast cells in log-phase growth (OD = 0.5) were concentrated 10 times (OD = 5) by a glass filter system with a 0.45 μm filter (Millipore). Cells were exposed to a final osmolyte concentration of either 0.2 M or 0.4 M NaCl ...
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bioRxiv - Bioengineering 2021Quote: ... then rinsed 3 times with PBS and permeabilized with staining buffer (0.1% Triton X-100/PBS, Sigma, 93443) for 1 hour before immunolabeling.
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed three times with PBS and mounted in fluorescence mounting medium (Mowiol 4-88, Sigma Aldrich). Immunofluorescence microscopy was performed using the confocal microscopes LSM 780 and LSM 800 Airyscan (both from Carl Zeiss ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were rinsed 3 times in 1X PBS and permeabilized with 0.1% Triton X-100/PBS (Sigma, 93443) for 15 min at RT ...
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bioRxiv - Bioengineering 2020Quote: ... Patchoulol was identified by comparison of retention time and mass spectra with a primary reference standard (Sigma-Aldrich) and was quantified using a standard curve (R2 > 0.99).
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bioRxiv - Biochemistry 2020Quote: ... Membranes were quickly washed 3 times with TBS/T (0.1% Tween20, Sigma #P5927; 20mMTris-HCL pH 7.6, T7943, Sigma, 150mM NaCl, Sigma) then stained with Ponceau S (PoncS ...
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bioRxiv - Neuroscience 2020Quote: ... Time pregnant female C57/Bl6 mice were injected with either saline or VPA (Sigma-Aldrich, 600mg/kg, subcutaneous) on E12.5 ...
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bioRxiv - Physiology 2020Quote: ... Cells were treated at dose and time indicated with palmitate or vehicle (BSA) using sodium palmitate (Sigma Aldrich) conjugated to BSA in ethanol at 65°C ...
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bioRxiv - Neuroscience 2021Quote: ... washed two times within PBS and PLA was realized according to the instructions of the manufacturer (DuoLink, Sigma). Primary antibodies used were as shown in Supplementary Table S4 ...
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bioRxiv - Neuroscience 2021Quote: ... the slices were washed with PBS three times and then permeabilized in 0.1% Triton X-100 (Sigma-Aldrich) for 15 min at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... The worms were washed 2–3 times with PBS-T and then stained with 0.125% thioflavin-S (Sigma) in 50% ethanol for 2 min and then de-stained sequentially in ethanol (50% ...
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Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor networkbioRxiv - Plant Biology 2021Quote: ... Beads were washed five times with immunoprecipitation wash buffer [GTEN extraction buffer with 0.3% (v/v) IGEPAL (SIGMA)] and re-suspended in 70μl SDS loading dye ...
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bioRxiv - Microbiology 2021Quote: ... The beads were washed with 1x Tris-buffered saline (TBS) three times and proteins were eluted with 55μL of 100μg/mL 3xFLAG peptide (Sigma) in 1x TBS ...
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bioRxiv - Microbiology 2021Quote: ... Wells were washed a final time and the assay was developed using the SigmaFast pNpp reagent (Sigma Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... tissues were washed 3 times in PBS and stored at 4°C in PBS containing 0.05% NaN3 (Sigma) until use ...
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bioRxiv - Microbiology 2021Quote: ... Concentrations were determined using external standards by comparing the retention time (all compounds were purchased from Sigma-Aldrich). Peaks were integrated using the EZChromElite software (Version V3.3.2.SP2 ...
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bioRxiv - Microbiology 2021Quote: ... The resulting liquid was aliquoted into 4 phase lock tubes (Quanta Biosciences) and extracted 3 times with an equal volume of phenol:chloroform:isoamylalcohol (25:24:1, Sigma). A final extraction with an equal volume of chloroform (Sigma ...
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bioRxiv - Pathology 2021Quote: Vibratome sections were washed three times with phosphate-buffered saline (PBS) containing 0.4% Triton X-100 (Sigma-Aldrich) (0.4% PBTx) ...
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bioRxiv - Cancer Biology 2020Quote: ... and HLA complexes subsequently eluted three times with 200 mL 1% trifluoracetic acid (TFA, Sigma/Aldrich, cat. # 02031). For the separation of HLA ligands from their HLA complexes ...
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bioRxiv - Developmental Biology 2020Quote: ... cells are washed 3 times with PBS and blocking solution is added (Bovine Serum Albumin, BSA. Sigma; A7906) at 3% in PBS for at least 30 minutes ...
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bioRxiv - Biophysics 2022Quote: ... The beads were washed three times with filtered PBS and incubated with nitrogen vacancy fluorescent nanodiamonds (798134, Sigma) in a 1:1 ratio for 10 minutes at room temperature ...
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The Batten disease protein CLN3 is important for stress granules dynamics and translational activitybioRxiv - Molecular Biology 2022Quote: ... Coverslips were then incubated with 200μl of primary antibody solution for 1 h at RT and washed 3 times with PBS before incubation with secondary antibody solution containing 0,1μg/mL DAPI solution (Sigma) for 1 h at RT ...
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bioRxiv - Biophysics 2022Quote: ... The fixed neurons were rinsed three times with PBS and permeabilized in 0.2% Triton X-100 (Millipore Sigma) in PBS for 5min ...
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bioRxiv - Cancer Biology 2022Quote: ... Mice were given 6 times weekly with a dose of 1.0 mg DMBA (Sigma-Aldrich, Milwaukee, WI, USA) in 0.2 ml of corn oil or an equal volume of corn oil alone (vehicle ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were diluted 10 times in 20 mM TCEP in 20 mM triethylammonium bicarbonate (TEAB, Sigma-Aldrich) buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were centrifuged a second time and pellets resuspended in 1 mL 0.25 % Triton X-100 (Sigma Aldrich) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... and dipped three times in the 25% w/v mixture of Bovine Serum Albumin (BSA) (B4287, Sigma Aldrich)) in the cell medium ...
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bioRxiv - Immunology 2021Quote: ... Subsequently cells were washed 3 times with PBS before being treated with 2% saponin (250 µL/well) (Sigma) in distilled water and incubated for 15 min at 37°C and 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the planarians were washed three times with IO water and homogenized in 1% Triton X-100 (Sigma-Aldrich) in PBS as previously described (Hagstrom et al ...
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bioRxiv - Cancer Biology 2022Quote: ... The membranes were washed again with TBST three times and visualized with chemiluminescence using HRP substrate (Millipore, #WBKLS0500) or Pico (Thermo Fisher ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were washed three times with 1x PBST and incubated in 1 mg/mL DAPI (Sigma-Aldrich D9542) diluted at 1:500 in 1x PBS at room temperature for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... The fixed cells were then washed three times with PBS and permeabilized with 0.3% Triton-X100 (Sigma-Aldrich) diluted in PBS for 15 min at rt ...
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bioRxiv - Neuroscience 2019Quote: ... After 3 times washing with PBS-T [PBS with 0.5% (v/v) Triton X-100 (Sigma Aldrich, # X100)] fixed brains were incubated with primary antibody containing 10% Normal Donkey Serum in 0.4% PBS-T overnight at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... The brains were then washed three times with PBS containing 0.5% Triton X-100 (PBST, Sigma-Aldrich, T8787); each time ...
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bioRxiv - Plant Biology 2019Quote: ... Identification was performed based on retention times and masses of salicylic acid and 4-chlorobenzoic acid standards (Sigma). The amount of SA was calculated on the basis of its relative response factor to the internal standard 4-chlorobenzoic acid at 1000 ng/mL ...
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bioRxiv - Molecular Biology 2019Quote: ... All experiments were repeated three times as triplicates on a white Greiner CELLSTAR 96-well plate (Sigma-Aldrich). Cel-miR-39-3p was used as a negative control.
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bioRxiv - Biochemistry 2019Quote: ... T47D and MDA-MB-468) two consecutive times in the presence of 6 μg/μl polybrene (Sigma-Aldrich). Cells were selected for 10 days with 2 μg/μl puromycin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... cells in stationary phase were diluted 100 times in YNB medium containing 5 µ g.mL−1 phleomycin (Sigma) and grown at 30°C with rigorous shaking (200rpm ...
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bioRxiv - Immunology 2019Quote: ... The supernatant was centrifuged at 12,000 rpm for 10 min each time by Amicon Ultra (UFC500308) from Millipore for protein concentrate ...
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bioRxiv - Molecular Biology 2020Quote: ... Bound protein was eluted three times for 30 min by incubation with 0.3 mg/ml 3xFLAG peptide (Sigma) in BC100 at 4°C with end-over-end rotation ...
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bioRxiv - Pathology 2020Quote: ... Membranes were washed three times as above and then developed with BCIP/NBT liquid substrate system (Sigma-Aldrich). The protein concentration of the FIV was determined using a BCA assay (Pierce #23227 ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatants were collected and concentrated ten times in an Amicon 10 k centrifuge filter (Millipore Merck, Darmstadt, Germany) following the manufacturer’s instructions.