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Citations for Millipore Sigma :
301 - 350 of 2489 citations for Rat TMEM165 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... MISSION® TRC shRNA Lentiviral Vectors (Sigma) were used.
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bioRxiv - Cell Biology 2021Quote: ... lentiviral particles were generated by transfecting HEK293T cells with 6 μg PLKO.1 scramble control and MISP-targeted shRNA plasmids (SIGMA; TRCN0000422523, TRCN0000116527), 4 μg psPAX2 packing plasmid (Addgene ...
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bioRxiv - Cell Biology 2021Quote: SDC3 was stably knocked down in C2C12 myoblasts by transfecting cells with a plasmid containing an shRNA sequence that targets SDC3 (Sigma, Mission # TRCN0000071990) to generate the SDC3-depleted (S3kd ...
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bioRxiv - Systems Biology 2023Quote: Non-replicating shRNA lentivirus were generated in HEK293FT cells transfected with MISSION plasmids procured from Sigma-Aldrich (details in Supplementary Table 2). Transfection involved the combination of MISSION plasmid (6 µg) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.8 µg pCMV-VSV-G (lentiviral envelope plasmid) and 2 µg purified shRNA DNA with 12 µg (3:1) of X-tremeGENE transfection reagent (Sigma Aldrich, #6366244001). Following 72 hours of transfection ...
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bioRxiv - Cancer Biology 2022Quote: Individual shRNAs were obtained from the MISSION shRNA Library from the RNAi Consortium TRC1.0 in the pLKO.1 vector (SIGMA):
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bioRxiv - Cell Biology 2019Quote: A GFP-expressing MDA-MB-231 cell line lacking functional Nav1.5 expression was produced previously by transduction of recombinant lentivirus for shRNA targeting Nav1.5 (MISSION pLKO.1-puro shRNA transduction particles; Sigma) (Nelson ...
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bioRxiv - Microbiology 2022Quote: The shRNA library originated from the Broad institute Genetic Perturbation platform and individual shRNA clones were purchased from Sigma in an pLKO or pLKO_TRC005 context (3 shRNA per genes for a total of 419 genes ...
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bioRxiv - Neuroscience 2021Quote: ... a craniotomy was performed under isoflurane anesthesia and 0.5 μL of lentiviral GPR83 shRNA or control shRNA particles (109; Sigma Mission Lentiviral Transduction Particles ...
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bioRxiv - Genomics 2022Quote: ... containing between three and five expression constructs each encoding target-specific 19-25 shRNAs or a single shRNA (Sigma Aldrich). HAP1 cells were plated at 120,000 cells per well (∼40% confluency ...
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bioRxiv - Cancer Biology 2020Quote: ... containing cGAS shRNA sequences (shcGAS #1, TRCN0000428336; shcGAS #2, TRCN0000149811) and non-target shRNA control vector (shScramble, SHC016) were purchased from Sigma.
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bioRxiv - Immunology 2020Quote: ... The pLKO vectors either encoded two specific shRNAs against mouse Akt1 (Cat# TRCN0000304683) or a non-silencing control shRNA sequence (Cat# SHC002) (all from Sigma). Akt1 shRNA and control non-silencing shRNA viruses were intratracheally instilled in mice in a volume of 50 µl ...
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bioRxiv - Neuroscience 2021Quote: ... HT22 cells were infected with lentivirus carrying the control scrambled-shRNA (5’-CCGGCAACAAGATGAAGAGCACCAACTCGAGTTGGTGCTCTTCATCTTGTTGTTTTTG-3’) or CB-specific shRNA (5’-CCGGGATTGGAGCTATCACCGGAAACTCGAGTTTCCGGTGATAGCTCCAATCTTTTTG-3’) with polybrene (Sigma) for two days ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 µg of lentiviral expression construct shRNA (pLKO.1 Mission shRNA DNA clone, Sigma-Aldrich Inc., St. Louis, MO, USA) control ...
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bioRxiv - Cancer Biology 2023Quote: ... pLKO.1 plasmids containing shRNAs targeting KCNN4 and control non-target sequences (Supplementary Table 1) were obtained from Sigma-Aldrich (St Louis, USA) and transfected into HEK293T cells with a packaging mix that contains a pCMV-dR8.91 (Delta 8.9 ...
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bioRxiv - Cell Biology 2020Quote: The ATF3 shRNA constructs were purchased from Sigma. The pSIN-3×flag-ATF3 vector was derived from pSin-EF2-Nanog-Pur (#16578 ...
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bioRxiv - Cell Biology 2020Quote: ... The non-mammalian targeting shRNA (Cat # SHC002V, Sigma) was used as a negative control ...
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bioRxiv - Microbiology 2022Quote: ... and GIGYF2 shRNA#3 (shGIGYF2#3) (Sigma, TRCN0000135088).
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bioRxiv - Cancer Biology 2019Quote: ... JUN and control shRNA were purchased from Sigma (their catalog numbers are listed in Supplementary Table S1) ...
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bioRxiv - Cancer Biology 2019Quote: TCF4 MISSION shRNA was purchased from Sigma-Aldrich (St ...
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The genetic status of IDH1/2 and EGFR dictates the vascular landscape and the progression of gliomasbioRxiv - Cancer Biology 2020Quote: ... Lentiviral vector to express shRNAs were: shCD248 (Sigma #SHCLNG-NM_020404 ...
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bioRxiv - Cell Biology 2021Quote: ... pLKO.1-puro non-target shRNA (Sigma-Aldrich) was used to produce the shCTL clone.
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bioRxiv - Cancer Biology 2022Quote: PRMT5 shRNAs in pLKOpuro vector were from Sigma sh1 (H3 ...
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bioRxiv - Molecular Biology 2022Quote: ... All constructs encoding shRNAs were purchased from Sigma.
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bioRxiv - Biochemistry 2022Quote: ... the pLKO.1 TRC1 shRNA library (SIGMA-ALDRICH) was used ...
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bioRxiv - Cancer Biology 2022Quote: ... and IRF3 shRNA-2 (Sigma-Aldrich, cat. TRCN0000005923). DNA for experiments was obtained using endotoxin-free plasmid purification kits (NucleoBond Xtra Midi EF ...
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bioRxiv - Cancer Biology 2022Quote: ... RELA/p65 shRNA-1 (Sigma-Aldrich, cat. TRCN0000014687); RELA/p65 shRNA-2 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... RELA/p65 shRNA-2 (Sigma-Aldrich, cat. TRCN0000014684); IRF3 shRNA-1 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... All other shRNA constructs were acquired from Sigma and shRNA sequence used ...
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bioRxiv - Neuroscience 2022Quote: ... and 4EBP1 shRNA constructs were purchased from Sigma: human TIMMDC1 (TRCN0000154913) ...
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bioRxiv - Neuroscience 2019Quote: ... or coding region (Rai1-shRNA #2: Sigma, TRCN0000328334) or scramble shRNA (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... lentiviral shRNAs with 6 μg/ml polybrene (Sigma) was added dropwise to the media ...
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bioRxiv - Physiology 2023Quote: ... shRNA lentiviral transduction particles were purchased from Millipore-Sigma to deliver and stably express shRNAs in MEFs ...
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bioRxiv - Cancer Biology 2023Quote: BMP6 shRNA (TRCN0000432077) was purchased from Sigma-Aldrich. Stable knockdown BMP6 JK43-P and JK43-M cells were selected with 5ug/ml puromycin.
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bioRxiv - Cancer Biology 2023Quote: ... shBRD4-L (#TRCN0000021424, Mission shRNA, Sigma-Aldrich Inc.) or shBRD4-S (#TRCN0000349782 ...
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bioRxiv - Cancer Biology 2023Quote: ... The other shRNAs were purchased from Sigma-Aldrich, with shRNAs against ZNF587B/814 and ZNF586 targeting the following sequences ...
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bioRxiv - Neuroscience 2024Quote: ... encoding an shRNA targeting TARDBP sequence (TRCN0000016038, SIGMA). Lentiviral particles were produced in the HEK293T cell line using psPAX2 (12260 ...
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bioRxiv - Cancer Biology 2023Quote: ... Mission shRNA clones were purchased from Sigma-Aldrich. The sequences of each shRNA are presented in Table S2 ...
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bioRxiv - Cancer Biology 2021Quote: ... two CSNK1D targeting shRNA constructs were selected from the Mission TRC shRNA library (TRCN0000023769, named shCSNK1D#1; TRCN0000361946, named shCSNK1D#2; Sigma-Aldrich); non-targeting scrambled shRNA served as control (shc002 ...
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bioRxiv - Cell Biology 2021Quote: ... validated lentiviral constructs (pLKO.1) expressing Mission shRNA targeting the following region 5’-GCTAGATTTACTAATGCCA-3’ in CPAP or scrambled shRNA were purchased from Sigma-Aldrich. For generation of stable cells ...
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bioRxiv - Molecular Biology 2022Quote: shRNA pLKO.1 plasmids expressing non-targeting control shRNA (NTC: SHC002) or anti- DDX39B shRNAs (Sh3: TRCN0000286976; and Sh5: TRCN0000294383) were purchased from Millipore Sigma. The construct pLCE-DDX39B was generated by cloning the coding sequence of DDX39B into the pLCE vector ...
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bioRxiv - Cancer Biology 2019Quote: ... The pLenti pLKO-non-target shRNA control vector (SHC002) and two different pLenti-mouse shRNA vectors for FYN were selected from Sigma Aldrich MISSION® shRNA Vectors ...
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bioRxiv - Neuroscience 2022Quote: ... We purchased a MISSION shRNA vector library encoding the microRNA-adapted shRNA targeting mouse Inka1 (Sigma-Aldrich, St. Louis, MO, USA). Among five shRNA clones (TRCN0000269004 ...
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bioRxiv - Cell Biology 2023Quote: The MISSION® pLKO.1-puro Non-Target shRNA (scr) and the shRNA against the UTR of human ZIP11 gene (5’-TCCTGATTGACTCTGATTATA-3’, Cat. TRCN0000434903) were from Sigma-Aldrich. The mammalian gene expression vector pLV[Exp]-EGFP/Neo-Ef1A (pLV ...
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bioRxiv - Cell Biology 2022Quote: ECs isolated from a single umbilical cord were split in two and transduced before the first passage with lentivirus encoding control or COL4A1 targeting shRNA (Sigma-Mission shRNA) (Bignon et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... we employed shRNA technology using the pLKO.1 (constitutive) or pLKO-3xLacO (inducible by IPTG) vector systems from MISSION® shRNA (Sigma Aldrich). In the constitutive system ...
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bioRxiv - Molecular Biology 2024Quote: ... cell lines were generated from parental mountain hare (LT1) and brown hare (LE3) cell lines by lentiviral transduction of shRNA (TRCN0000028617 and TRCN0000028579 MISSION® shRNAs, Sigma-Aldrich) followed by puromycin-resistant marker selection.
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bioRxiv - Cancer Biology 2021Quote: ... or shMCU (#SHCLNG-NM_138357 Mission shRNA, Sigma-Aldrich Inc.). 16hr post-transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected with shRNAs purchased from Sigma-Aldrich (shPTPRH-373 ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentiviral shRNA constructs for PPARGC1A were purchased from Sigma. Lentiviral particles were generated by co-transfecting HEK293T cells with packaging plasmids using the calcium phosphate method ...