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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Rat T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and co-transfection of heavy and light chain expression plasmids into 293-6E cells (National Research Council of Canada) using 25 kDa branched polyethylenimine (Sigma-Aldrich). After 6-7 days at 37°C and 6% CO2 under continuous shaking in FreeStyle Expression Medium supplemented with 20 U/ml penicillin and 20 µg/ml streptomycin (all Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: DRMs distribution on the cell surface was assessed by staining the cells with cholera-toxin coupled to FITC (CT-FITC, Sigma-Aldrich, Burlington, MA, USA). The target of cholera toxin is GM1 (monosialotetrahexosylganglioside) ...
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bioRxiv - Immunology 2019Quote: Purified CD4+ T cells were incubated for 5 days with 10 nM rotenone (Sigma) or DMSO control after which time the cells were used for metabolic assessment ...
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bioRxiv - Cell Biology 2021Quote: HeLa T-REx cells were seeded onto coverslips coated with poly-L-Lysine (Sigma) one day before staining ...
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bioRxiv - Biophysics 2022Quote: Jurkat T cells (ATCC TIB-152) were grown in RPMI (Sigma Aldrich, Madison, WI) while mouse thymoma BW5147 cells were cultured in Joklik-modified Minimum Essential Medium (JMEM (Sigma Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... T cells were fixed with 1.5% formaldehyde solution (F1635, Sigma-Aldrich, St. Louis, MO), washed ...
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bioRxiv - Genomics 2019Quote: The cancer-derived human CD8+ T-lymphocyte cell line My-La CD8+ (Sigma-Aldrich) was cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Immunology 2022Quote: T cell capacity to proliferate upon stimulation with phytohemagglutinin-L (PHA-L) (Sigma Aldrich) was measured following guidelines of the Click-iT® EdU cell proliferation assays kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... 105 CD8+ T cells were treated overnight with 5 µM GSK-J4 (Sigma-Aldrich) and co-cultured with 100 bacteria per cell (BpC ...
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bioRxiv - Immunology 2020Quote: ... T cells were stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (Sigma), 1 mM ionomycin (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNAs from isolated CD4+ T cells were obtained using TRI Reagent (Sigma Aldrich). Total RNA concentration and quality were measured by Nanodrop (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... to evaluate transmigration of T-cells towards the chemoattractant SDF-1α (Sigma-Aldrich, Singapore) for 16 h ...
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bioRxiv - Immunology 2022Quote: We labeled approximately 1 million CAR T cells with PKH67 green fluorescent dye (Sigma) and 1 million NALM-6 cells with PKH26 red fluorescent dye (Sigma ...
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bioRxiv - Systems Biology 2023Quote: ... We dissociated hiF-T-iPSCs from MEF feeder cells with accutase (Sigma #A6964-100ML) for 2-10 min at 37°C before adding embryoid body (EB ...
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bioRxiv - Immunology 2023Quote: ... The reaction was stopped by adding 9 ml T cell medium (RPMI 1640 (Sigma) supplemented with 10 % heat-inactivated FBS ...
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bioRxiv - Immunology 2023Quote: ... All organs were collected on ice in complete T cell medium (RPMI 1640 (Sigma), 10% heat-inactivated FCS (Atlanta Biologicals) ...
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bioRxiv - Immunology 2023Quote: ... HH7-2tg-FoxP3hCD2 T cells were isolated using collagenase VII (100U/ml, Sigma Aldrich) for 1h at 37°C and subjected to flow cytometric analysis ...
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bioRxiv - Immunology 2023Quote: ... T cells were cultured for 4 hours with PMA (50 ng/ml; Sigma-Aldrich), ionomycin (500 ng/ml ...
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bioRxiv - Biochemistry 2019Quote: ... 0.6 µM GTP-gamma-S in the presence of 60 Units of Thrombin (Sigma-Aldrich T1063-1KU). The sample was then passed through a Ni-agarose column (3 ml ...
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bioRxiv - Cancer Biology 2023Quote: ... gamma-h2AX levels were measured for conditions indicated in figures using manufacturer’s protocol (Sigma, no. 17-344). 200k cells were used per replicate per condition and assay was run in technical triplicate per condition.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Osteoclast resorbed surface (Oc.S/BS) was measured following TRAP staining with Leukocyte Acid Phosphatase TRAP Kit (Sigma Aldrich, catalog # 387A-1KT). RNA was extracted from isolated whole femur for qPCR for osteoblast (Col1a1 ...
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bioRxiv - Biophysics 2021Quote: ... Surfaces were coated with 10 μg/mL fibronectin (Sigma Aldrich) for 1 hour.
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bioRxiv - Cell Biology 2020Quote: ... Surfaces were then blocked with 0.2% Pluronic F-127 (Sigma), washed ...
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bioRxiv - Neuroscience 2020Quote: ... all equipment and surfaces were cleaned with RNaseZap (Sigma-Aldrich). Replicates of hiPSC-derived neurons were harvested at three different timepoints of differentiation (days 1 ...
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bioRxiv - Biochemistry 2022Quote: ... the surface was treated with tetraethyl orthosilicate (Sigma-Aldrich, USA), cyclohexane (Deajung ...
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bioRxiv - Plant Biology 2022Quote: ... seeds were surface sterilized and sown on MS medium (Sigma) containing 0.8% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... all instruments and surfaces were treated with RNaseZAP (Sigma, Germany) to block ribonuclease activity.
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bioRxiv - Biophysics 2020Quote: ... The surfaces were rinsed with PBS (Sigma-Aldrich, D8537-500ML) before incubating the chambers with blocking solution (10 g/l BSA (ThermoFisher ...
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bioRxiv - Biophysics 2023Quote: ... Surfaces were then treated with a 1% gluteraldehyde (Sigma Aldrich) in milliQ H2O solution for 10 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: Plasma cytokines and cytokines secreted in cell culture media were measured using ELISA (Luminex 200; Millipore) by following the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Cytokine and chemokine levels were measured with a commercial rat cytokine/chemokine magnetic bead panel 96-well plate assay kit (Milliplex MAP kit, Merck Millipore), which detects 5 cytokines and chemokines including IP-10/CXCL10 ...
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bioRxiv - Cancer Biology 2021Quote: ... and rat IgG (Sigma).
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bioRxiv - Immunology 2021Quote: ... α-Rat IgG (Sigma) was administered to control WT mice in the same manner.
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bioRxiv - Cell Biology 2020Quote: ... rat anti-HA (Sigma) 1:200 ...
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bioRxiv - Cancer Biology 2019Quote: ... Anti-Interferon-α/β Receptor Chain 2 Antibody (IFNA2, clone MAB1155; Millipore) and its corresponding isotype (Mouse IgG2a ...
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Q-SNARE Syntaxin 7 confers actin-dependent rapidly replenishing synaptic vesicles upon high activitybioRxiv - Neuroscience 2020Quote: ... neurons were incubated with 10 nM recombinant TeNT–light chain (Sigma–Aldrich) for 16 h in a CO2 incubator ...
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bioRxiv - Pathology 2020Quote: The light chain 3-phosphatidylethanolamine-conjugate (LC3)-II (Sigma Aldrich, 1:3000) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Physiology 2020Quote: ... alkaline phosphatase-labeled goat antibodies against mouse IgM μ chain (Sigma-Aldrich) and mouse IgG1 γ chain (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... or 1:3000 dilution of anti-human IgM (μ-chain-specific) (Sigma) antibody subtypes and 1:6000 dilution of anti-human IgG1 ...
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bioRxiv - Immunology 2020Quote: ... Amplified S-gene and polymerase chain reaction (PCR) engineered pET31b(+) (Novagen, Germany) bacterial expression vector were amplified using 0570F and 0571R primers ...
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bioRxiv - Immunology 2022Quote: ... 1 μM Rotenone and Antimycin A (electron transport chain inhibitors) (Sigma-Aldrich). Non-mitochondrial respiration (OCR values following injection of Rotenone and Antimycin A ...
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bioRxiv - Molecular Biology 2023Quote: Bioactive lipids t-2-hex and t-2-hex-H2 were purchased from Sigma and applied directly to yeast cultures from 1mg/ml stock solutions in DMSO ...
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bioRxiv - Neuroscience 2022Quote: ND7/23 cells (a mouse neuroblastoma × rat dorsal root ganglion neuron hybrid cell line; Sigma−Aldrich, ECACC 92090903), were cultured in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Cell Biology 2021Quote: ... Cells on the surface of the plate were then lysed in the Triton lysis buffer (1% TritonX-100 [Sigma-Aldrich T8787] ...
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bioRxiv - Developmental Biology 2022Quote: Proteins used in surface plasmon resonance and crystallography were derived from stable cell lines established by G418 selection (1 mg/mL, Sigma) of transfected HEK293S GnTI(- ...
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bioRxiv - Cell Biology 2020Quote: ... cell-surface mannoproteins were stained by 1 mg/mL fluorescein isothiocyanate (FITC)-conjugated concanavalin A (Sigma-Aldrich, St. Louis, MO) in P buffer (10 mM sodium phosphate and 150 mM NaCl [pH 7.2] ...
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bioRxiv - Bioengineering 2020Quote: ... incubated 30 seconds in plasma cleaner then coated with cell-specific adhesive surface: for at least 1 hour with 10 μg/ml recombinant human fibronectin (Sigma) for Hela and SHSy-5Y cells ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining biotin on the cell surface was stripped with 50 mM of sodium 2-mercaptoethanesulphonate (MESNa) (M1511, Sigma-Aldrich) in PBS/CM at 4°C for 30 min followed by quenching with 5 mg/ml iodoacetamide (093-02152 ...
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bioRxiv - Neuroscience 2022Quote: ... Currents of 5-HT3A receptors and engineered constructs expressed at the cell surface were evoked by applying 10 μM serotonin creatinine sulfate complex (Sigma), or in short 5-HT ...
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bioRxiv - Biophysics 2019Quote: ... Dead cells were removed on day 6 after T cell isolation through density-dependent gradient centrifugation (Histopaque 1119, Sigma) and experiments were conducted between day 7 and day 9 after isolation.