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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for Rat Heat Shock 60 kDa Protein 1 Chaperonin HSP60 HSPD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Elution fractions containing the protein were pooled and concentrated (Amicon Ultra 100-kDa cut off, Millipore Sigma) for gel filtration (GF) ...
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bioRxiv - Biochemistry 2022Quote: ... The refolded protein was concentrated with Amicon Ultra-15 Centrifugal Filter Units (100 kDa NMWL, Merck Millipore) and remaining urea removed with a PD10 desalting column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... GGG-azide labelled proteins were concentrated to 25 μM on a 30 kDa Amicon Tube (Merck Millipore) in Tris/NaCl buffer and next labelled with 100 μM DBCO-Cy5 (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... The purified protein was further concentrated on Amicon Ultra Centrifugal Filters with 50 KDa cutoff (Sigma-Aldrich).
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein were pooled and concentrated (Amicon Ultra 10 kDa cut off, Millipore Sigma) for gel filtration ...
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bioRxiv - Biochemistry 2020Quote: ... The purified proteins were concentrated to 30-40 mg/mL using Amicon concentrators (30-kDa cutoff; Millipore).
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bioRxiv - Biochemistry 2020Quote: ... Protein complex was incubated overnight on ice and then concentrated onto 30 kDa MWCO Amicon concentrator (Millipore) until protein concentration reached 11 mg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein were pooled and concentrated (Amicon Ultra 100 kDa cut off, Millipore Sigma) for gel filtration ...
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bioRxiv - Microbiology 2021Quote: ... Eluted recombinant proteins were concentrated in Amicon Ultra-4 centrifugal filter units with 30-kDa cutoff (Millipore), concentrations were determined using the DC BCA protein assay (BioRad) ...
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bioRxiv - Immunology 2021Quote: ... the protein elution fractions were pooled and concentrated with 50 kDa molecular weight cutoff ultrafiltration unit (Millipore), then 1 mg of TEV protease was used per 50 mg protein forcleavage during dialyzing to the buffer with 250 mM NaCl ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein was washed and concentrated using 30 kDa Amicon centrifugal filter unit (Millipore Sigma CAT#UFC8030).
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bioRxiv - Biochemistry 2022Quote: ... Protein-containing fractions were pooled and concentrated using centrifugal ultra-filtration devices (10 kDa cut-off; Millipore). Protein concentration was determined using a Bradford assay with a standard curve calibration with BSA ...
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bioRxiv - Microbiology 2023Quote: ... the reduced proteins were transferred to a 30 kDa Microcon filter unit (YM-30 filter units, Millipore) and centrifuged at 14,000 × g for 20 min in all consecutive steps ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated by ultrafiltration using an Amicon Ultra-0.5 mL (10 kDa cutoff, Merck Millipore) at 14,000 × g and 4°C for 15 min ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated using Amicon Ultra centrifugal filter units with 30 kDa MWCO (Millipore, Darmstadt, Germany). Protein concentrations were measured in a Nanodrop spectrophotometer using the theoretical extinction coefficients calculated using the Expasy ProtParam tool ...
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bioRxiv - Neuroscience 2023Quote: ... Protein-containing fractions were pooled and concentrated on an Amicon 3 kDa MWCO spin concentrator (Merck Millipore). After another IMAC purification step using Ni-NTA resin ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated using Amicon Ultra-15 centrifugal filters (100 kDa cut-off, Merck-Millipore). After treatment with 1 mM diamide for 15 min to completely oxidize the protein ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were pooled and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated sample was loaded onto a HiLoad Superdex S200 (16/600 ...
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bioRxiv - Biochemistry 2023Quote: ... The RafH protein fraction was pooled and concentrated in a 10 kDa cut-off Amicon filter (Millipore). The protein concentration was checked by measuring absorbance at 280 nm ...
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bioRxiv - Biophysics 2023Quote: ... The protein solution was concentrated using a 30 kDa cutoff centrifugal filter unit (Millipore, Burlington, MA, U.S.A.) and loaded into a Superdex200 10/300 GL gel filtration column (G.E ...
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bioRxiv - Cancer Biology 2023Quote: ... Fractions containing monodisperse protein were concentrated in a 100 kDa cut-off Amicon ultra centrifugal filters (Millipore) and flash frozen in liquid nitrogen for further assays ...
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bioRxiv - Immunology 2023Quote: ... The reduced protein solutions were then processed using Amicon Ultra Centrifugal Filters (10 kDa MWCO, Millipore Sigma) to remove TCEP ...
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bioRxiv - Biochemistry 2023Quote: ... The protein peak was collected and concentrated using a 10 kDa Amicon Ultra 15 concentrator cell (Millipore), and then applied to a Superdex 200 16/600 pg size exclusion column equilibrated with 25 mM Tris pH 7.5 ...
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bioRxiv - Bioengineering 2024Quote: ... Glycosidases were removed after incubation by filtration through 10 kDa protein-binding EZ™ filters (Millipore Corporation). N-glycans were then analysed by UPLC as previously described (36).
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bioRxiv - Physiology 2020Quote: ... and serum osteopontin (OPN) by Rat Single Plex Kit (Millipore). All the assays were performed in accordance with manufacturer protocols.
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bioRxiv - Biochemistry 2024Quote: ... blood plasma was collected at 9 am and ELISA kits were used following manufacturer’s instructions (Leptin: Crystal Chem, 90030; GLP-1: Sigma, EZGLP1T-36K ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasma insulin and C-peptide were measured with mouse/rat ELISAs (EZRMI-13K and EZRMCP2-21K, respectively, EMD Millipore, Billerica, MA, USA). Plasma non-esterified fatty acids (NEFA ...
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bioRxiv - Microbiology 2020Quote: ... Biotinylated proteins were captured in ELISA plates pre-coated with 5 µg/mL of Avidin (Sigma) in Na2CO3/NaHCO3 buffer pH 9.6 ...
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bioRxiv - Microbiology 2020Quote: ... CCL2 and CCL5 protein levels were quantified using a sandwich ELISA procedure (Sigma, Cat# RAB0055 & RAB0077) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... 10 kDa (Merck Millipore) in storage buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... brPEI (25 kDa, Sigma) at an optimum N/P ratio of 10 was mixed with pDNA solution in nuclease-free water ...
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bioRxiv - Cell Biology 2021Quote: ... Thyroglobulin (660 kDa) (Sigma) was used as a molecular weight standard ...
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bioRxiv - Molecular Biology 2020Quote: ... 60 μL (50% slurry) of Protein-RIPA-equilibrated FLAG-m2 beads (Sigma-Aldrich, Cat. No A2220) were added together with 1 μL of Benzonase (Merck-Millipore ...
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bioRxiv - Microbiology 2023Quote: ... Then the supernatant was separated by size to a large (>3 kDa) and small (<3 kDa) fraction using Amicon ultrafiltration system with 3 kDa filter (Millipore, Ireland). The fraction then filtered again through a 0.22 µm (Millipore).
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bioRxiv - Cell Biology 2019Quote: ... and supplemented with 10% (v/v) 1 kDa dialyzed FBS (Sigma-Aldrich), 1% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... Permeability of 70 kDa dextran (0.1 mg mL-1, FD70, Sigma Aldrich) was measured exactly as previously described29 ...
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bioRxiv - Neuroscience 2019Quote: ... and sodium fluorescein (NaFluo, MW 0.365 kDa, 1%, 0.04 ml, Sigma-Aldrich) were each dissolved in sterile saline and administered via the arterial femoral catheter as single bolus injections ...
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bioRxiv - Neuroscience 2019Quote: ... together with TRITC-dextran (MW 65 kDa, 0.05 ml 1%,, Sigma-Aldrich) for delineation of the vasculature.
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bioRxiv - Neuroscience 2023Quote: ... FITC-dextran 40 kDa (FD40) (Sigma, FD40S-250MG; 25 mg ml-1) or 45 kDa Texas Red-conjugated ovalbumin (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... dextran (FITC-conjugated, 70 kDa, 0.1 mg mL-1, #FD70, Sigma Aldrich), Trastuzumab (Alexa Fluor 488-conjugated ...
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bioRxiv - Cancer Biology 2023Quote: ... 200 kDa subunit (NF-H) (1:500, Sigma, Saint Louis, MO, #N0142), and neurofilament ...
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bioRxiv - Biochemistry 2023Quote: ... concentrated to ∼1 mL with the 3 kDa cutoff spin filter (Millipore). Untagged γ-TuNA was then further purified over a HiLoad 16/600 Superdex 75 column pre-equilibrated in gel filtration buffer (40 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... p-IRS1 r307 (1/800, 185 kDa; 05-1087, Sigma-Aldrich, USA), p-AKT (1/800 ...
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bioRxiv - Cancer Biology 2024Quote: ... or FITC-Dextran (2000 kDa, FD2000s, Sigma Aldrich, 5 mg mL-1) was injected in the tail vein to visualize the brain vasculature ...
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bioRxiv - Microbiology 2021Quote: ... for ELISA (Sigma) was added to visualize the reaction ...
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bioRxiv - Neuroscience 2022Quote: Proteins were resolved by SDS-PAGE and proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were suspended in pH-Shock Buffer (10 mM Tris, pH 7.5, 50 mM sucrose, 10 mM CaCl2, 0.33% Proteinase Inhibitor Cocktail (Sigma)) ...
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bioRxiv - Microbiology 2023Quote: ... cells were subjected to alternating hyperosmotic shocks of 400 mM sorbitol (Sigma-Aldrich, Cat. #S1876, dissolved in LB) and recovery without sorbitol ...
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bioRxiv - Cell Biology 2024Quote: ... ∼695 mOsm hyper-osmotic shocks (204%) were performed by adding 1mL of 1.5x solution containing 96.9g/L D-mannitol (Sigma) to the medium.
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bioRxiv - Neuroscience 2024Quote: ... to perform immunohistochemical procedures in the Penn Digital Neuropathology Lab as described previously.21,61 Immunohistochemistry for neuronal markers included antigen retrieval using citrate buffer and heat for neuronal nuclei protein (NeuN) enriched in most neurons (Anti-NeuN mouse monoclonal antibody, clone A60,1:1000, Millipore) and heavy non-phosphorylated neurofilament (NF-H ...