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Citations for Millipore Sigma :
451 - 500 of 976 citations for RT qPCR Master Mixes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The following antibodies were used for ChIP-qPCRs following dCas9-KRAB targeting: anti-FLAG (Sigma: F-3165), anti-CTCF (Abcam ...
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bioRxiv - Bioengineering 2019Quote: qPCR products were separated and visualised on 2% agarose gels stained with 0.25μg/mL ethidium bromide (Sigma).
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bioRxiv - Genomics 2021Quote: ... Fold change in gene expression (ΔΔct method) was evaluated by qPCR (iTaq universal SYBR Green Supermix) (Sigma), performed in CFX96RTS thermal cycler (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative reverse transcription PCR reactions used the 2xKiCqStart® SYBR® Green qPCR ReadyMix™ (Sigma-Aldrich). PCR consisted of an initial activation step at 95°C for 3 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Real-time detection of miRNA expression was performed by using MystiCq miRNA qPCR Assay Primer (Sigma-Aldrich), using Universal Mouse Reference RNA and U6 as reference for mouse and human cells ...
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bioRxiv - Genetics 2022Quote: ... and cDNA was diluted 1/50 for qPCRs using KAPA SYBR FAST (Sigma-Aldrich Co LTD, KK4610). RT-qPCR was carried out on a Roche LC480 for 40-45 cycles.
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bioRxiv - Developmental Biology 2023Quote: ... The primers for the qPCR reaction were purchased from “KiCqStart® SYBR® Green Primers” (Sigma-Aldrich) and the gene gadph was chosen as a control.
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bioRxiv - Biochemistry 2024Quote: ... Cell lines were verified to be mycoplasma-free with the LookOut Mycoplasma qPCR detection kit (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2023Quote: ... qRT-PCR was performed on Bio-Rad CFX96 real time (qPCR) system using predesigned KiCqstart primers (Sigma) and TBB green (#RR-208A ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were run under standard conditions using KOD Hot Start Master Mix (Sigma-Aldrich: 71842). After subsequent ligation with T4 ligase (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... Xist and Gapdh RNA expression was performed using FS Universal SYBR Green Master (Rox) (Sigma-Aldrich) according to manufacturer’s instructions under the following conditions ...
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bioRxiv - Neuroscience 2021Quote: ... using the SYBR™ Green master mix (life technologies) and predesigned primers (KiCqStart® Primers, Sigma). Relative gene expression levels were normalized to β-actin in each sample with the ΔΔCT method.
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bioRxiv - Microbiology 2020Quote: ... A region of pol (44, 45) was amplified using KOD Hot Start Master Mix (Millipore, 71842) in a first round of PCR with forward primer ...
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bioRxiv - Systems Biology 2020Quote: ... 4μl of a master mix containing 2.5μl of 1M triethylammonium bicarbonate (TEAB, Millipore Sigma T7408-100ML), 1.3μl of 200ng/μl trypsin (Promega Trypsin Gold ...
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bioRxiv - Cancer Biology 2021Quote: ... The antibody master-mix was then filtered through a pre-wetted 0.1-μm spin-column (Millipore) to remove antibody aggregates ...
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bioRxiv - Bioengineering 2021Quote: ... the substrate (silicon master or glass slide) was first cleaned in strong soap (SDS, Sigma-Aldrich) and washed thoroughly with water ...
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bioRxiv - Microbiology 2022Quote: ... Eighty µl of a master reagent mix (20 µl bicine buffer (1M, pH 8, Sigma-Aldrich), 10 µl H2O ...
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bioRxiv - Plant Biology 2023Quote: ... The total of 10 mL master mix solution was made of 10 µL of Luminol (Sigma) from 100 mM stock solution in DMSO ...
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bioRxiv - Synthetic Biology 2023Quote: ... KAPA SYBR FAST polymerase master mix was purchased from Sigma-Aldrich (St. Louis, MI, United States).
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bioRxiv - Microbiology 2023Quote: ... Each 20 µl PCR reaction contained 10 µl of JumpStart RedTaq DNA Polymerase Master Mix (Sigma), 8 µl dH2O ...
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bioRxiv - Biophysics 2023Quote: ... the master was placed into a solution of propylene glycol methyl ether acetate (PGMEA, Sigma-Aldrich), which helped in the development process ...
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bioRxiv - Microbiology 2024Quote: Overnight cultures from each master plate were grown in Mueller-Hinton broth (MH) (Sigma-Aldrich, UK) and diluted 1:100 in MH ...
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bioRxiv - Microbiology 2024Quote: ... Each reaction contained 12.5 µL of Roche FastStart Essential DNA Probes Master (Sigma-Aldrich, Oakville, Canada), 0.4 µM each invA primers ...
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bioRxiv - Systems Biology 2020Quote: ... secondary antibody was added (Goat anti-rabbit HRP, 1% milk, TBST 0.2%, 2 h, RT, 1:5000; Sigma). Once washed ...
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bioRxiv - Immunology 2021Quote: ... and then developed at RT by addition of 100 μL of p-nitrophenyl phosphate (pNPP) substrate (Sigma-Aldrich) per well ...
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bioRxiv - Neuroscience 2020Quote: ... followed by blocking for 20 min at RT in 5% normal donkey serum (Sigma, Cat N° D9663-10ML) in 0.1% PBS-Triton X-100 ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were washed three times in 1x PBS before quenching with 0.1 M glycine (Roth) in 1x PBS for 10 min at RT and 0.1 % NaBH4 (Sigma) in 1x PBS for 7 min at RT ...
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bioRxiv - Neuroscience 2020Quote: ... slices were washed in PBS at room temperature (RT) then permeabilized with PBS-T (0.1M PBS + 0.2% Triton-X100, Sigma) for 30 mins ...
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bioRxiv - Neuroscience 2020Quote: ... counterstained with DAPI (diluted 1:1000 in PBS) for 3 minutes at RT and mounted with Fluoromont (Sigma).
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bioRxiv - Microbiology 2021Quote: ... followed by staining of cell nuclei for 3 min at RT with 1 μg/ml Hoechst 33342 (Sigma) in PBS−/− ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed again with blocking solution at RT and DNA was counterstained with DAPI (Sigma-Aldrich, D9564). For EdU staining ...
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bioRxiv - Immunology 2022Quote: ... in PBS 30 minutes at RT and blocked with 2% BSA and 5% Donkey Serum (Sigma-Aldrich, D9663) in PBS during 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated with secondary antibodies for one hour at room temperature (RT) and mounted using Mowiol (Sigma); Hoechst (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were incubated for 2 h at RT with anti-αSMA antibody (1:200, Sigma Aldrich, C6198-100UL) and Goat anti Rabbit Alexa Fluor 647 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... cryo-sections were acetylated using 0,1 M triethanolamine and 0,25% acetic anhydride for 25 min at RT (respectively #90275, #A6404, Sigma). This steps are followed by a pre-hybridization using 2X of SSC ...
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bioRxiv - Cell Biology 2020Quote: ... are stained for 5 minutes at room temperature RT with 0.5μl of PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling (#P7333, Sigma) and then polished with the exosome spin columns (#4484449 ...
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bioRxiv - Bioengineering 2021Quote: ... at RT and nuclear counterstaining was performed using 4’,6-diamidino-2-phenylindole (DAPI, 1.5 μg/mL; Sigma). After brief drying ...
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bioRxiv - Bioengineering 2019Quote: ... Miller.) Reactions were carried out in an RT assay buffer containing: 60 mM Tris (77-86-1, Sigma), 30 mM KCl (7447-40-7 ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 minutes each with PBST at room temperature (RT) with agitation and blocked with 5% Milk Powder (Sigma) in PBST for 1 hr at RT with agitation ...
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bioRxiv - Developmental Biology 2020Quote: ... Fish were stained overnight at room temperature (RT) with a solution of 0.1% Alcian blue 8GX (Sigma Aldrich) w/v ...
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bioRxiv - Immunology 2021Quote: ... in 3% BSA-containing DPBS for 15 min at RT and stained with phalloidin-TRITC (P1951, Sigma Aldrich) and DAPI (D9542 ...
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bioRxiv - Microbiology 2019Quote: ... RT-PCR was performed on 100 ng of cDNA in triplicate using SYBR Green Jumpstart Taq readymix (Sigma). Primers RMC379/380 (16S internal) ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated for 30 min at RT with anti-β-actin (1:10’000, A3854 Sigma-Aldrich), which is used as a normalizer.
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bioRxiv - Genomics 2019Quote: ... blots were rinsed three times in PBS and incubated for 10min at RT in 0.02% methylene blue (Sigma) in 0.3M sodium acetate ...
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bioRxiv - Cell Biology 2022Quote: ... followed by incubation for another 1 hour at RT in a solution containing 2.5% potassium ferrocyanide (Sigma-Aldrich) in 0.1 M PIPES ...
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bioRxiv - Cell Biology 2022Quote: ... and fixed 10 minutes at RT in fixation buffer (10% formaldehyde solution, Sigma Aldrich, in nuclease free water). Cells were washed 2 x and permeabilized O/N in 70% ethanol at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 2% paraformaldehyde for 15 min at RT then permeabilised using 0.5% saponin (Sigma-Aldrich) and stained for 30 min at 4°C for the following intracellular markers ...
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bioRxiv - Neuroscience 2022Quote: ... containing coverslips were fixed for 20 min at room temperature (RT) in 4% (w/v) paraformaldehyde (PFA, Sigma) in 0.15 M phosphate buffer (potassium dihydrogen phosphate (Merck ...
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bioRxiv - Neuroscience 2022Quote: Digoxygenin (DIG)-labeled probes were produced with the SuperScriptTM III One-Step RT-PCR kit (Sigma 12574-026) using specific primers for the Bgl-FaNaC transcript (Forward ...
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bioRxiv - Pathology 2023Quote: Liver and renal cortex RNA from EU-exposed mice were extracted using RNAzol RT reagent (Sigma-Aldrich, # R4533). The incorporated EU was detected by click chemistry biotinylation and dot blotting ...