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Citations for Millipore Sigma :
401 - 450 of 5406 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... 1 hour blocking was performed at RT using 5% Bovine Serum Albumin (BSA, Sigma) in PBS-T (PBS with 0.1% Triton-X) ...
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Cold exposure drives weight gain and adiposity following chronic suppression of brown adipose tissuebioRxiv - Physiology 2021Quote: ... RT-qPCR was carried out as previously described using rat-specific oligonucleotide primers (Sigma) or FAM-MGB Taqman probes [42] ...
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bioRxiv - Physiology 2020Quote: Frozen muscle powder was homogenized using RNAzol RT reagent (Sigma-Aldrich, St Louis, MO) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 4′,6-diamidino-2-phenylindole (DAPI, Sigma #D9542, 1:1000 for 10min at RT) Mice were deeply anesthetized with isoflurane and then transcardially perfused with ice-cold heparinized PBS (25U/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... at RT for 2 h and BM purple alkaline phosphatase substrate (Sigma-Aldrich, 1144207001) was used to visualize the color in the humid chamber ...
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bioRxiv - Bioengineering 2022Quote: ... antigen retrieval was done with Pronase (1mg/mL for 10 minutes at RT, Sigma) followed by blocking with BSA (3% w/v ...
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bioRxiv - Neuroscience 2022Quote: ... Blocking was performed at RT with 2% bovine serum album (BSA) (Sigma-Aldrich, #A7906), 2% goat serum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: RT-qPCR was performed with the SYBR Green JumpStart Taq Ready Mix (Sigma – S4438) and custom-made primers (Supplementary Table 4) ...
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bioRxiv - Immunology 2022Quote: ... the cells suspension was reconstituted in RT CV medium/10μM Y-27632 (Sigma Aldrich) and 150’000 cells/cm2 were added to the prepared collagen I hydrogels ...
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bioRxiv - Microbiology 2023Quote: ... for 60 min at RT and alkylated with 40 mM iodoacetamide (IAA, Sigma-Aldrich) in the dark for at least 60 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... for an hour at RT after permeabilization with 0.2% Triton X-100 (Sigma-Aldrich) in PBS for 35 minutes at RT ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilised for 20 min at RT using PBS/0.25% Triton X-100 (Sigma). Cells were blocked for 30 min at RT with blocking solution (PBS-0.25% Triton X-100 plus BSA 1%) ...
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bioRxiv - Cell Biology 2022Quote: ... for 15 min at RT and permeabilized in 0.25% Triton X-100 (Sigma-Aldrich) for 20 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was cooled overnight at RT and filtered through 0.22 µm filter (Millipore). The insoluble PG sacculi were collected on the filters ...
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bioRxiv - Molecular Biology 2023Quote: ... permeabilized for 1 h at RT in PBS + 0.3% Triton X-100 (Sigma-Aldrich) (PBST) ...
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bioRxiv - Physiology 2023Quote: ... for 1h at RT and developed using Immobilon™ Western Chemiluminescent HRP Substrate (Millipore). Detection and quantification of chemiluminescence intensities were quantified by using ChemidocTM imaging system and Image Lab 5.2.1 software (BioRad) ...
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bioRxiv - Developmental Biology 2023Quote: ... and (3) 3 hrs at RT in 0.1% Direct Red 23 (212490, Sigma-Aldrich), in PBST ...
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bioRxiv - Molecular Biology 2024Quote: ... for 20 min at RT and permeabilised in permeabilization buffer [1% BSA (Sigma Aldrich) and 0.2% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... in PBS (PBST) at RT followed by blocking solution (PBST + 4% BSA (Sigma A9647)) ...
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bioRxiv - Immunology 2024Quote: ... for 10 min at RT and blocked with 1% BSA (Sigma-Aldrich, MA, USA) in PBS for 30 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were fixed for 10 minutes at RT with 4% formaldehyde (Merck, Sigma Aldrich) diluted in PBS 1X (Gibco) ...
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bioRxiv - Plant Biology 2021Quote: ... PCR reactions were performed with the Readymix Red Taq PCR reactive mix (Sigma-Aldrich), Phusion high-fidelity DNA polymerase (Fermentas ...
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bioRxiv - Microbiology 2019Quote: ... PCR reactions were performed with the Readymix Red Taq PCR reactive mix (Sigma-Aldrich) or with the Phusion high-fidelity DNA polymerase (Fermentas ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR was performed using REDTaq® ReadyMix™ PCR Reaction Mix (R2523, Merck-SIGMA) using previously published primers (Charalambous et al ...
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bioRxiv - Microbiology 2021Quote: ... Cell lines used in this study were negative for mycoplasma contamination as determined with Lookout Mycoplasma PCR Detection Kit (Millipore Sigma). During the course of this study ...
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bioRxiv - Molecular Biology 2020Quote: ... All cell lines tested negative for mycoplasma infection and were routinely tested afterwards using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich). A549 cells (ATCC CCL-185 ...
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bioRxiv - Cell Biology 2021Quote: ... The gDNA extractions and PCR verifying integration of the fluorescent tags into the genomic loci was performed using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). PCR amplicons were run on 1 or 2% agarose gels in TBE and gels were imaged with Bio-Rad Gel ChemiDoc XRS system and Quantity One software.
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Systems Biology 2022Quote: ... and cDNA fragments were amplified by PCR according to the manufacturer’s protocol (GenomePlex Single Cell Whole Genome Amplification Kit, Sigma Aldrich WGA4). The amplified libraries were run on a bioanalyzer to assess quality (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... ear notches were collected from mouse pups to extract genomic DNA using the RED Extract-N-Amp Tissue PCR Kit (Sigma-Aldrich). Ear notches were incubated in the tissue preparation (25 μl/sample ...
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bioRxiv - Pathology 2022Quote: ... Pf2-HA_OE and pf2-HA_KO ChIP and input control samples) was recovered from Proteinase K treated samples using the GenElute PCR purification kit (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2019Quote: ... Yolk sacs from each of the dissected embryos were collected and genomic DNA preparation was done using Extract-N-Amp tissue PCR kit (Sigma-XNAT2). Placenta tissues were collected in RLT buffer and RNA was extracted using RNAeasy Mini Kit (Qiagen – 74104) ...
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bioRxiv - Developmental Biology 2019Quote: Genomic DNA samples were prepared using tail tissues or embryonic tissues from the mice using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). Genotyping was done using REDExtract-N-Amp PCR ReadyMix (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... All cell lines tested negative for mycoplasma infection and were routinely tested afterwards using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines including stable clones were routinely evaluated for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma-Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... and fed with StemFlex media (ThemoFisher Scientific) and undergo monthly mycoplasma testing to ensure cultures are mycoplasma-free with the LookOut Mycoplasma PCR detection kit (Millipore Sigma). For the differentiation of iPSC-derived microglia-like cells ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lines tested negative for mycoplasma contamination using a PCR-based method using the VenorGeM Classic Mycoplasma Detection Kit (Sigma-Aldrich). STR-genotyping was performed to confirm the identity of HeLa-JVM ...
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bioRxiv - Developmental Biology 2023Quote: ... or ear biopsies (3 week old pups) was used for PCR based genotyping using the REDExtract-N-Amp kit (Sigma-Aldrich) with the primers listed in STable5 ...
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bioRxiv - Cancer Biology 2023Quote: ... All cells were regularly tested for mycoplasma contamination using the LookOut mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO, CA).
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bioRxiv - Developmental Biology 2023Quote: ... Library preparation was then performed using 200 ng of 4C-template DNA with the Expand Long Range PCR kit (Millipore, 4829042001) under specific PCR conditions (94 °C for 2 min ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T cells used for proteome-derived peptide library generation were tested every six months for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... Working stocks were screened to ensure the absence of mycoplasma every 3 months using LookOut Mycoplasma PCR Detection Kit (Cat. No. D9307 Sigma, UK). Cells were cultured in Modified Eagle’s Medium supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR1 products for each tumor were individually column-purified with a GenElute™ PCR Clean-Up Kit (Sigma-Aldrich NA1020-1KT). For PCR2 ...
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bioRxiv - Cell Biology 2023Quote: ... elegans genomic DNA extracted from 8-10 adult worms using the Extract-N-Amp™ Tissue PCR Kit (Millipore Sigma, MA) as previously described38 then sequenced ...
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bioRxiv - Bioengineering 2023Quote: ... All the cell lines were regularly tested for mycoplasma contamination using the LookOut Mycoplasma PCR detection kit (Product no. #MP0035 Sigma-Aldrich) and maintained in humidified cell culture incubators at 37°C and 5% CO2.
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted from mice ear tissues (or tail for embryos) using Extraction « Extract-N-Amp PCR » kit (Sigma #XNAT2-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: DNA from larval tails and juvenile and adult tail fins was extracted using the Extract-N-Amp Tissue PCR Kit (Millipore Sigma). After verifying SNP location with Sanger sequencing at Azenta Life Sciences ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR sex genotyping reactions were performed in a final volume of 25 μl with the following reagents and volumes per sample: 12.5 μl KAPA2G Fast HotStart DNA Polymerase (5 U/μL) from the KAPA2G Fast HotStart PCR Kit (Sigma-Aldrich, KK5601), 1 μl of 12.5 μM Ube forward primer and 1 μl of 12.5 μM Ube reserve primer ...
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bioRxiv - Biochemistry 2024Quote: ... R-domain phosphorylation sites and interacting residues mutants were generated by site-directed mutagenesis using a Q5-New England Biolabs PCR mutagenesis kit together with primers synthesized from Millipore sigma and verified by sequencing (Elim Biopharmaceuticals ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were confirmed to be free of mycoplasma contamination (VenorTMGeM Mycoplasma Detection Kit, PCR-based, Sigma Aldrich Cat #MP0025). Cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM ...