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Citations for Millipore Sigma :
401 - 450 of 4043 citations for RSPO3 Human HEK293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
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bioRxiv - Biochemistry 2021Quote: α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-Cul2 (EPR3104 ...
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bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
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bioRxiv - Microbiology 2023Quote: N-terminal His-tagged KtrD cloned into pRSFDuet-1 (Novagen) was overexpressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Anti-His primary antibody (H1029) was purchased from Sigma Aldrich, HRP conjugated secondary antibody (J1430 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% heat-inactivated Fetal Bovine Serum (HI-FBS; Sigma-Aldrich), 1% v/v penicillin-Streptomycin (VWR) ...
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bioRxiv - Cancer Biology 2023Quote: ... which were cryopreserved in HI FBS with 10% DMSO (Sigma). Cells were directly transferred to a -80 °C freezer in Coolcell freezing containers (Corning ...
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bioRxiv - Biochemistry 2023Quote: ... 25 uL of (His)6-tagged TEV-protease (Sigma-Aldrich) was added and the solution incubated overnight at 4°C.
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% HI-horse serum and vancomycin (Sigma, USA). The inoculated flask was then kept in shaking conditions (100 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... and BRAF NTs/ BRAF KD with His antibody (Sigma SAB5600227). Finally ...
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bioRxiv - Molecular Biology 2024Quote: ... One milliliter of equilibrated HIS-Select Nickel Affinity Gel (Sigma) was added to the cleared cell lysate and incubated with mild agitation for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3% heat-inactivated fetal bovine serum (HI-FBS; Sigma) at 37°C for 15 ∼ 30 min ...
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bioRxiv - Microbiology 2024Quote: ... and heat-inactivated serum (HI-FBS; Sigma F9665, Lot# BCCB5091) was aliquoted and stored at −20°C until required.
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bioRxiv - Cell Biology 2023Quote: ... human ephrin-B2-Fc (10 µg/ml, RnD Systems, #7397-EB) was mixed with Cy3-conjucated Fc-antibody (5 µg/ml, Sigma-Aldrich, # C2571) in HBSS and incubated for 30-60 minutes at room temperature with gentle agitation ...
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bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...
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bioRxiv - Cancer Biology 2021Quote: ... complemented with 20% fetal calf serum (FCS) (Sigma-Aldrich), 1% pen/strep (Gibco ...
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bioRxiv - Immunology 2021Quote: ... with 2% foetal calf serum (FCS, Sigma-Aldrich, UK). Lymphoprep (d = 1.077 g mL−1 ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma, F7524) at 37°C with 5% CO2 ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 15% fetal calf serum (FCS) (Merck Millipore), 0.1mM b-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma Aldrich), 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2019Quote: ... Media was also supplemented with 10% dialysed FCS (Sigma), penicillin (100 IU/mL) ...
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bioRxiv - Molecular Biology 2019Quote: ... containing 10% fetal calf serum (FCS; Sigma, Vienna, Austria), 2 mM L-glutamine (Lonza) ...
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bioRxiv - Cell Biology 2020Quote: ... supplement with 10% fetal calf serum (FCS, Sigma, US) at 25°C ...
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich), and penicillin–streptomycin (Gibco) ...
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bioRxiv - Bioengineering 2021Quote: ... and fluorinated oil (Fluorinert FC-40) (Sigma Aldrich, F9755) treated media (Figure 3a ...
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bioRxiv - Cell Biology 2021Quote: ... containing 10% FCS and 1% GPS solution (Sigma-Aldrich) after ZF probe administration at 5 μM ...
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bioRxiv - Plant Biology 2021Quote: ... Fusicoccin (FC, 1mM stock in 96% ethanol, Sigma Aldrich), 5-Adamantyl-IAA (pico-cvxIAA ...
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bioRxiv - Immunology 2021Quote: ... or anti-mouse IgG (Fc-specific)-HRP (Sigma, A2554) secondary antibody respectively ...
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bioRxiv - Microbiology 2021Quote: ... and supplemented with 10% fetal calf serum (FCS; Sigma), 100 units/ml penicillin and 100μg/ml streptomycin (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% (v/v) FCS (Sigma-Aldrich, F7524), 1% penicillin-streptomycin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... and 10% Fetal Calf Serum (FCS, Sigma-Aldrich, Austria) in T25 polystyrene cell culture flasks for suspension cells (Sarstedt ...
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bioRxiv - Biochemistry 2022Quote: ... supplemented with 10% fetal bovine serum (FCS) (Sigma-Aldrich), 1% L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were suspended in 10 mL of FCS (Sigma). After centrifugation at 500 g for 5 min at room temperature (RT) ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich) and 2 mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Cells were cryopreserved (90% FCS, 10% DMSO [Sigma-Aldrich]) in liquid nitrogen and thawed in complete medium as required on the day of use.
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bioRxiv - Microbiology 2022Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich,), 10◻mM HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich) and 1% Penicillin-streptomycin (P/S ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 10% foetal calf serum (FCS, Sigma-Aldrich), 100 U/mL penicillin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 5% foetal calf serum (FCS; Sigma-Aldrich) for staining ...
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bioRxiv - Cell Biology 2019Quote: ... Media was also supplemented with 10% dialysed FCS (Sigma), penicillin (100 IU/mL) ...
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bioRxiv - Microbiology 2020Quote: ... Nanosota-1C-Fc was cloned into pET42b vector (Novagen) with a C-terminal human IgG1 Fc tag ...
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bioRxiv - Neuroscience 2019Quote: ... supplemented with 10% Foetal Calf Serum (FCS, Sigma-Aldrich), 50 U/ml penicillin (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: hACE2-Fc was digested with thrombin protease (Sigma Aldrich) in the presence of 2.5 mM CaCl2 at a 1:300 w/w thrombin:protein ratio ...
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bioRxiv - Microbiology 2021Quote: ... and 5% FCS (Sigma-Aldrich, St. Louis, MO, USA) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... and/or 2.5 μg/ml E-cadherin-Fc (Sigma). IELs were plated in RPMI-T medium containing 2 μg/ml PerCP-e710 conjugated anti-CD107a (1D4B ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma Aldrich), 100 μg/ml streptomycin and 100 U/ml penicillin (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... and 10% FCS (Sigma-Aldrich, St. Louis, Missouri, USA)) assuming that they double within 24 hours ...
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bioRxiv - Immunology 2023Quote: ... mixed in 2% fetal calf serum (FCS; Sigma-Aldrich). Cells were stained with combinations of the following fluorophore-conjugated anti-mouse monoclonal antibodies ...
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bioRxiv - Immunology 2023Quote: ... 10% fetal calf serum (FCS) treated with Chelex (Sigma) to removed endogenous calcium ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich) and 2 mM L-glutamine (Sigma-Aldrich ...