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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for RFamide Related Peptide 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and 0.8 mg/mL FLAG peptide (Sigma-Aldrich; F3290) at 4°C for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... or 10 μg/ml OVA peptide 323-339 (Sigma) at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting peptides were desalted with C18 ZipTips (Millipore) for LC-MS/MS analysis.
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bioRxiv - Immunology 2023Quote: ... with 50µg peptide + 50µL Common Freund’s Adjuvant (CFA, Sigma) along with 50µL PBS for a total volume of 100µL ...
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bioRxiv - Developmental Biology 2023Quote: ... 3X FLAG Peptide-coupled beads (Sigma Aldrich, F4799-4MG) were pre-washed with lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and eluted with 0.2 mg/ml 3xFLAG peptide (Millipore). All samples were denatured in Laemmli buffer and analyzed by SDS-PAGE (4-20% TGX ...
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bioRxiv - Molecular Biology 2024Quote: ... The peptide mixtures were desalted using C18 ZipTips (Millipore) and analyzed by liquid chromatography-mass spectrometry (LC-MS).
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bioRxiv - Microbiology 2023Quote: ... Peptide mixtures were then desalted by μZipTipC18 tips (Millipore) before nano-liquid chromatography-electrospray ionization-Orbitrap tandem mass spectrometry (nLC-ESI-Q-Orbitrap-MS/MS ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were either desalted with C18 ZipTips (Millipore Sigma) or Pierce Peptide Desalting Spin Columns (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... were resolved on a 12.5% SDS gel and puromycin-labelled peptides were detected with anti-puromycin (1:5000 mouse anti-puromycin, clone 12D10; Sigma). Prior to antibody detection ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C) twice with 300 μL of Elution buffer (Lysis buffer A with 1 mg/mL 3 × FLAG peptide (Sigma)) ...
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bioRxiv - Neuroscience 2020Quote: ... Sandwich ELISA kits for quantitative detection of human Aβ 1-40 peptides in a flexible 96 well format were purchased from Millipore (cat ...
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bioRxiv - Neuroscience 2020Quote: ... These were formed by 24h incubation at 37°C of a 1mg/ml solution of Aβ 1-42 peptide (Sigma) in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... The beads were washed three times with 0.5 ml of FLAG wash buffer and the bound protein was eluted with 1X SDS-PAGE loading buffer supplemented with 100 µg·ml-1 3X FLAG peptide (Sigma-Aldrich) on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... Dynabeads following the first pulldown were resuspended in Re-ChIP elution buffer (20 mM Tris-Cl pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 100 µg of 3X FLAG peptide [Sigma, #F4799]), and incubated in 4 °C for 1 hour with rotation to selectively elute FLAG-fusion protein ...
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bioRxiv - Molecular Biology 2022Quote: ... FLAG-tagged proteins were eluted by 15 minutes shaking (500 RPM) in 25 μl of B100 + HALT containing 1 mg/ml 3xFLAG peptide (Sigma) at room temperature ...
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bioRxiv - Physiology 2020Quote: ... For insulin clearance during an insulin challenge, mice were given human insulin (1 U/kg, NovoRapid) or human C-peptide (50 µg/kg, Sigma) by intraperitoneal injection and blood samples were collected by tail vein sampling at 0 ...
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bioRxiv - Biochemistry 2022Quote: ... rPAI-1 was further purified by binding to anti-FLAG agarose beads and elution with 3X FLAG peptide (Millipore Sigma). rPAI-1 was quantified spectrophotometrically (ε280nm = 43,000 M−1 cm−1) ...
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bioRxiv - Neuroscience 2022Quote: ... peptides were acidified with 2 μl of 1% TFA then desalted using C18 ZipTips® (Millipore, cat no. Z720070-96EA).
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bioRxiv - Biochemistry 2023Quote: ... Anti-Flag M2 beads were washed five times with 1 ml buffer A and then eluted with buffer A supplemented with 3xFlag peptide (Sigma). Unphosphorylated ACKR3 ...
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bioRxiv - Biochemistry 2023Quote: ... The column was washed with 10 column volumes of FLAG buffer and proteins were eluted as 1 ml fractions with 100 μg/ml FLAG peptide (Sigma). 5 mM DTT was added to each fraction and peak fractions as determined from Coomassie blue gel electrophoresis were pooled and concentrated in Vivaspin 15-max speed filter (MWCO 30kD ...
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bioRxiv - Immunology 2023Quote: ... Samples were then washed with lysis buffer containing 0.1% DDM and incubated with lysis buffer containing 1% DDM and 3×Flag peptide (100 µg/ml, Sigma #F4799) overnight for elution ...
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bioRxiv - Biophysics 2023Quote: ... 5% CO2 with serial dilutions of G51 peptide or 1% DMSO solution in full culture medium and then stimulated with concanavalin A (ConA, Sigma) in a final concentration 2.5 μg/ml for 5 days ...
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bioRxiv - Immunology 2023Quote: ... followed by digestion to peptides using 1 µg trypsin at 37°C for 18 hr and the digestion was stopped using 20% TFA (Sigma) to pH 2 to 3 ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... both the excised control spots and the peptide membranes were incubated with HRP-tagged anti-Biotin antibody (1:10,000, Sigma-Aldrich) and washed thrice again with Binding Buffer ...
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bioRxiv - Cell Biology 2021Quote: ... protein was eluted by incubating 1 CV of elution buffer (10 mM Tris, 200 mM NaCl, 1 mM DTT, 1 mM EGTA, 0.2 mg/mL FLAG peptide (Sigma-Aldrich, St. Louis, MO, US, SAB1306078-400UL), pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Serum insulin and c-peptide levels were measured by insulin or C-peptide ELISA respectively following the manufacture’s protocol (Sigma Aldrich, St. Louis, MO, USA). Mouse metabolic testing and fasting methods followed established protocols [16,33,34].
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bioRxiv - Immunology 2022Quote: Sequences for Mtb proteins Rv1196 and Rv0125 were obtained from publicly available databases and peptide libraries were generated using a commercially available peptide library design tool (PEPscreen, Millipore Sigma, Merck, Darmstadt, Germany) with peptide lengths of 20 amino acids (a.a. ...
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bioRxiv - Cancer Biology 2023Quote: ... was synthesized using Fmoc solid-phase peptide synthesis (Liberty Blue™ Peptide Synthesizer; CEM, Matthews, NC) with a Rink Amide MBHA resin (EMD Millipore, Burlington, MA) as previously described.61
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were extracted with 5% formic acid (Sigma F0507-1L), then 5% formic acid with 75% ACN ...
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bioRxiv - Molecular Biology 2021Quote: ... Peptides were then desalted on ZipTip C18 pipette tips (Millipore), lyophilized to dryness ...
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bioRxiv - Microbiology 2020Quote: ... peptides were desalted using C18 ZipTips (Merck Millipore, Darmstadt, Germany) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... and eluted with 2 mg/ml HA peptide (Sigma-Aldrich) at 37 °C for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein complexes were eluted with 3xFlag peptide (200ug/mL, Sigma) in 5 column volumes Buffer 2 for 1 hour at 4C ...
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bioRxiv - Immunology 2019Quote: ... and incubated with rabbit polyclonal anti-2A-peptide (EMD Millipore), anti-VSV-G or anti-actin (SantaCruz ...
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bioRxiv - Cell Biology 2019Quote: ... Human c- peptide ELISA kit (cat # EZHCP-20K, Millipore MA), Human Insulin ELISA kit (cat # ELH- Insulin ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG beads were eluted using 3X FLAF peptide (Sigma-Aldrich).
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bioRxiv - Systems Biology 2019Quote: ... Enriched peptides were resuspended in 50 mM ammonium bicarbonate (Sigma) and subjected to a second digestion with trypsin for 2 hours per the manufacturer’s recommendation ...
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bioRxiv - Physiology 2019Quote: ... Peptides were bound and desalted using C18 ZipTips (Merck Millipore) and washed with 0.1% trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were then purified using a C18 ZipTip (Merck Millipore). Eluted peptides were dried in a SpeedVac and resuspended in 10 µL of 0.1 % formic acid ...
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bioRxiv - Neuroscience 2021Quote: ... Beads were then resuspended in TBS with Flag peptide (Millipore) in final volume of 50 μl and left at 4°C on a rotating wheel for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and proteins were eluted using a 3xFLAG peptide (Sigma #F4799). Samples were then heated to 100°C for 10 minutes in SDS sample buffer and analyzed by western blotting.
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bioRxiv - Plant Biology 2020Quote: ... Peptides were purified using Zip tip C18 pipette tips (Millipore) according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2020Quote: ... The peptides were reconstituted in 0.1% formic acid (Sigma-Aldrich) and quantified using peptide assay kit ...
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bioRxiv - Neuroscience 2021Quote: ... The Anti-Amyloid Peptide β (A1976-25UG, Sigma-Aldrich, India), raised in rabbit ...
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bioRxiv - Biochemistry 2022Quote: Anti-FLAG M2 agarose and FLAG peptide were from Sigma, Ni-NTA agarose was from Qiagen ...
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bioRxiv - Biophysics 2022Quote: ... Peptides were desalted using C18 microcolumns (ZipTip® C18, Millipore), vacuum dried and resuspended in 20 μL of 0.1% formic acid.
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bioRxiv - Molecular Biology 2020Quote: ... TRF2-containing complexes were released with a FLAG peptide (Sigma). Samples were resolved by SDS-PAGE ...
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bioRxiv - Physiology 2021Quote: ... Labeled peptides were pooled and purified using C18 columns (Sigma) and eluted with 70% acetonitrile containing 0.1% formic acid.
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bioRxiv - Biochemistry 2021Quote: ... The resulting peptides were desalted with ZipTip C18 (Millipore, USA), washed with 0.2% (v/v ...