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Citations for Millipore Sigma :
601 - 650 of 10000+ citations for RFamide Related Peptide 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The resulting peptide mixture was desalted using C18-Zip tips (EMD Millipore, Billerica, MA), speed-vacuumed to dryness and suspended in 0.1% formic acid for injection into ...
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bioRxiv - Neuroscience 2023Quote: ... The precipitated sample were eluted with 100 μg/ml 3XFLAG peptide (Sigma-Aldrich, F4799). The eluted samples were split in half for reducing (mix with 5x SDS sample buffer at 100°C for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted in two column volumes of 0.2 mg/ml 3xFLAG peptide (Sigma) and dialyzed against 50 mM HEPES pH 7.4 ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 µg of peptides were further desalted with C18 ZipTips (Merck Millipore, Darmstadt, Germany) to ensure complete removal of beads.
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bioRxiv - Molecular Biology 2023Quote: ... VCF1 C-terminal peptide stocks (150 μM) were prepared in pure DMSO (Sigma Aldrich). Threefold serial dilutions of VCF1 proteins or peptides were prepared in the SPR running buffer from the primary stocks starting from highest concentration of 300 nM (VCF1 C-terminal peptides ...
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bioRxiv - Physiology 2023Quote: ... Peptides from each of these fractions were cleaned up using C18 ZipTip (Millipore, USA). Each fraction was then analysed on a quadrupole-TOF hybrid mass spectrometer (TripleTOF 6600 ...
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bioRxiv - Cell Biology 2023Quote: ... The recovered tryptic peptides were resuspended in 0.1% trifluoroacetic acid (Sigma-Aldrich; cat. #T6508) and desalted with ZipTips C18 Pipette Tips (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted using 10 mM glutathione or 0.5 mg/mL 3XFLAG peptide (Sigma) in Lysis Buffer with 2 mM DDM.
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bioRxiv - Cell Biology 2022Quote: ... Peptides were eluted in 10 µl 5 % DMSO in LC-MS water (Sigma Aldrich) in an ultrasonic bath for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were eluted twice with 2 μg/μl V5 peptide in water (Sigma-Aldrich) or 1 μg/μl 3x FLAG peptide (ApexBio ...
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bioRxiv - Synthetic Biology 2023Quote: ... peptide samples were loaded onto an Ascentis® ES-C18 Column (Sigma–Aldrich, USA) and were eluted from the column by using a 10 minute gradient from 98% solvent A (0.1 % FA in H2O ...
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bioRxiv - Immunology 2023Quote: ... and protein complexes were eluted with 150 mg/ml of 3X FLAG peptide (Sigma). Samples were diluted in 2X SDS sample buffer and analyzed with SDS–PAGE according to standard procedures ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were identified by indirect immunofluorescence using an anti-myc antibody (C3956, Sigma-Aldrich). SW13-human adrenocarcinoma vimentin-deficient cells ...
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bioRxiv - Biochemistry 2023Quote: ... 15 µg of peptide was incubated with 50 µl of 33 mM iodoacetamide (Sigma) in degassed 5% formic acid (pH 4 ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were eluted in 10 µL 5% DMSO in LC-MS water (Sigma Aldrich) in an ultrasonic bath for 10 min.
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bioRxiv - Systems Biology 2024Quote: ... Samples containing 4 µg of peptides were de-salted using C18 Zip-tips (Millipore) and resuspended in 20 µl of 2.5 % (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... the immune complexes were eluted with 150 ng/µL 3×FLAG peptide (Sigma-Aldrich) in TBS solution for 30 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Samples were eluted in 45 µL of FLAG peptide (300 µg/mL, Millipore Sigma) in Wash Buffer plus 0.05% RapiGest (Waters Corp ...
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bioRxiv - Microbiology 2024Quote: ... Bound protein was eluted with 100 µL of 1.5 mg/mL FLAG peptide (Millipore) resuspended in wash buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The TaPOR2 gene lacking the part coding a transit peptide and pET15b plasmid (Novagen) were amplified with PCR using Q5 polymerase (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were eluted with 50 µg/mL of 1x FLAG peptide (Sigma-Aldrich #F3290), concentrated using 30 kDa centricons (Millipore #UFC9030) ...
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bioRxiv - Plant Biology 2021Quote: ... washed 5 times and then eluted with 3xFLAG peptide ([0.1 mg/mL]; Sigma, Cat# F4799). The crosslinking was reversed overnight at 65 °C and purified DNA (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 mM NaCl) and eluted with 300 ng/L of FLAG peptide (#F4799, Sigma-Aldrich) for four hours under moderate agitation 62 ...
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bioRxiv - Microbiology 2020Quote: ... bound proteins from the pooled washed resin were eluted with 100μg of FLAG peptide (Sigma) in FLAG wash buffer ...
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bioRxiv - Genomics 2020Quote: ... The beads were washed extensively and were eluted with 100 μg/ml 3xFLAG peptide (Sigma). The eluates were incubated with TRIzol reagent followed by isopropanol precipitation and DNase I digestion (Fermentas) ...
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bioRxiv - Molecular Biology 2021Quote: ... The HA-tagged PGBD1 was eluted by 500 μl 100 μg/ml HA-peptide (Sigma) in 100 mMNaCl ...
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bioRxiv - Developmental Biology 2019Quote: ... Peptides were dissolved in a small amount of 0.1% trifluoroacetic acid (302031, Sigma-Aldrich; TFA), followed by purification with the use of ZipTip (MA01821 ...
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bioRxiv - Biochemistry 2019Quote: ... The native proteins without signal peptides were expressed in BL21(DE3) Gold competent cells (Novagen). The gene encoding the LtgA deletion mutant lacking the alpha helix 503(ATAREIAGKIGMD)513 was chemically synthesized by ProteoGenix ...
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bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). All immunoprecipitations were performed on three independent biological replicates.
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bioRxiv - Biochemistry 2020Quote: ... 100 mM NaCl) and eluted with 50 μg/ml (final concentration) of 3xFLAG peptide (Sigma) in TGE ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in 40 µl lysis buffer + β-mercaptoethanol and 0.5 mg/mL 3xFLAG-peptide (Sigma) and incubated 30 minutes at 4°C to dissociate proteins bound to the FLAG-beads ...
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bioRxiv - Biochemistry 2020Quote: ... The mRNA-cyclic peptide libraries were eluted with 600 µL of 0.1% DEPC (Millipore Sigma) water containing 1 mM DTT ...
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bioRxiv - Biochemistry 2020Quote: ... The dried peptide films were dissolved in 200 μL Dimethyl sulfoxide (DMSO) >99.9% (Sigma-Aldrich), vortex for 1 min and sonicated for 1 min ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant INO80 complex was eluted with buffer B containing 1.6 mg Flag Peptide (Sigma-Aldrich). Anion exchange chromatography (MonoQ 5/50 GL ...
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bioRxiv - Neuroscience 2020Quote: ... a synthetic opioid peptide with high MOR specificity and clonidine HCl (Sigma, St Louis, MO). These compounds were aliquoted and concentrated using a Speed Vac and stored at −20°C before experimental procedures ...
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bioRxiv - Physiology 2021Quote: ... and 3X FLAG® Peptide (F4799) were obtained from Sigma Aldrich (St-Louis, Missouri, USA). The PKC inhibitor Gö6983 (ab144414 ...
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bioRxiv - Biochemistry 2021Quote: ... The MERS-CoV peptide was labeled with sub-stoichiometric FITC (Sigma-Aldrich, St. Louis, Missouri) in a basic HEPES buffer (pH 8.2 ...
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bioRxiv - Microbiology 2020Quote: ... the sequence encoding mature proteins without the signal peptide was cloned into pET-14b (+) (Novagen) to generate the expression vector pET14b-6xHis-BcCrh1 ...
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bioRxiv - Systems Biology 2020Quote: ... The dried peptides were reconstituted in 60 μL of 0.1% trifluroacetic acid (TFA) (Sigma-Aldrich). Peptides were then fractionated using SCX StageTip method as described earlier [23] ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins were eluted two times with 600 μL 250ug/mL 3xFLAG peptides (SIGMA, F4799) in TBS [50mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: Anti-FLAG-M2 affinity agarose gel and 3X FLAG peptide were obtained from Sigma Aldrich, n-dodecyl β-D-maltoside (DDM ...
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bioRxiv - Biophysics 2020Quote: ... The remaining FLAG beads were incubated with 150 ng/μL 3X FLAG peptide (Millipore-Sigma) in kinase assay buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The remaining FLAG beads were incubated with 150 ng/μL 3X FLAG peptide (Millipore-Sigma) in kinase assay buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Systems Biology 2022Quote: ... Purification and concentration of peptides was performed using C18 Zip Tip Solid Phase Extraction (Millipore). Peptides recovered from in-gel digestion processing were reconstituted into a final concentration of 0.5μg/μL of 2% ACN ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plasma insulin and plasma c-peptide were measured using radioimmunoassay (EMD Sigma, Cat #: RI-13K) and bead immunoassay (Luminex ...
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bioRxiv - Systems Biology 2022Quote: ... Purification and concentration of peptides was performed using C18 Zip Tip Solid Phase Extraction (Millipore). The peptides recovered were reconstituted into a final concentration of 0.5μg/μL of 2% ACN ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged proteins were eluted by 60 μl of 3x FLAG® Peptide (Sigma F4799) (diluted in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2021Quote: ... 100 μl of elution buffer (150 μg/ml 3X-FLAG peptide, Sigma, in Buffer 2) was added and samples were subjected to end-over-end rotation at 4 °C for 30 min ...
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bioRxiv - Biophysics 2020Quote: ... Protein was eluted with Myosin Buffer supplemented with 100 μg/mL Flag peptide (Sigma-Aldrich). The eluent was buffer-exchanged to Storage Buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were eluted with 300 μl of 250 μg/ml 3xFLAG peptide (Sigma, cat#F4799) in IP buffer without NP40 for 30 min at 25°C ...