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Citations for Millipore Sigma :
401 - 418 of 418 citations for RAP1GAP siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: PMA differentiated THP-1 cells were transiently transfected with 100nM targeted siRNA using low molecular weight polyethyleneimine (Sigma-Aldrich, 40872-7). Further ...
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bioRxiv - Cancer Biology 2023Quote: ... and HOC7 (75,000 cells/ml) cells were transfected with either control siRNAs (Catalog # 12935146, Thermo Fisher Scientific; Catalog # SIC001, Millipore Sigma) or CaMKK2 siRNAs (50nM ...
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bioRxiv - Cell Biology 2023Quote: ... OM SVF Lin–/TM4SF1+/MSLN– cells as well as cells subjected to siRNA-mediated knockdowns 48h post-transfection were collected into TRIzol (Sigma, #T3934). The direct-zol RNA kit (Zymo Research #R2062 ...
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bioRxiv - Bioengineering 2023Quote: The effects of MALAT1 siRNA on hMSCs proliferation were characterized with a cell proliferation assay (Cell Counting Kit-8, CCK-8 assay, Sigma Aldrich). hMSCs were seeded in a flat-bottom 96-well tissue culture plate at a concentration of 2000 cells per well with a volume of 100 µL ...
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bioRxiv - Molecular Biology 2023Quote: ... 5ill-3ill GGUCUAAAGGUGGAGAUA[dT][dT] and UUAUCUCCACCUUUAGACC[dT][dT]) and non-targeting control (scRNA (MISSION® siRNA Universal Negative Control #1, SIC001)) were both obtained from SIGMA and used to assess the impact of the tag on endogenous AGO2 silencing capacity ...
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bioRxiv - Neuroscience 2024Quote: ... Controls included the library internal negative controls ath-miR416 and cel-miR-243 as well as pooled non-specific siRNAs [60,61] that were transfected into cells and then either treated with DMSO (Sigma, D4540-500ML), with 0.7 µM CCCP ...
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bioRxiv - Cell Biology 2024Quote: ... a complex of siRNA and DharmaFECT 2 was prepared in serum-free DMEM (Sigma-Aldrich, St. Louis, MO, USA, Cat# D5796) at a volume of 100 μL/well according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: XB2 keratinocytes transfected with siRNAs (Supplementary Table 1) or incubated with 25 nM of a Rab7 inhibitor (CID-1067700, Sigma-Aldrich) were then fed with 0.1 g/L of melanocores for 4 hours and melanin was chased for another 20 hours ...
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bioRxiv - Biochemistry 2019Quote: ... Knock-down of glutamate dehydrogenase 2 (GDH2, encoded by the GLUD2 gene) using siRNAs (#NM_012084: 5’CUAACCUCUUCACGUGUAA’3 and 5’UUACACGUGAAGAGGUUAG‘3, Sigma-Aldrich, Taufkirchen, Germany) or transfection of GLUD2 plasmid/empty vector controls was done using Lipofectamine RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... 5’-GACAUAUUUGAUAAACUUAAA-3’, 5’-GUUAUCAGUCUGAGCCAGG-3’), human DRP1 (5’-GCAGAAGAAUGGGGUAAAU-3’, 5’-UCCGUGAUGAGUAUGCUUU-3’, 5’-GAGGUUAUUGAACGACUCA-3’) non-targeting scrambled siRNA (Sigma, SIC001-1NMOL) for 48-72 hours using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... RAD21mAC-OsTIR cells were reverse-transfected with 20 µM FLUC control esiRNA or esiRNA custom designed to SA1 exon31 or SA2 exon32 (MISSION® siRNA, Sigma Aldrich) using RNAiMAX (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with either non-targeting siRNA or si-Adipor1 or si-Adipor2 plasmid using lipofectamine 3000 reagents (Sigma-Aldrich, USA) as per manufacturer protocol ...
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bioRxiv - Cell Biology 2020Quote: Flp-In™293 cell lines seeded in 12-well plates were transfected with individual siRNAs (50 nM, Sigma, Extended Data Table 11) using Lipofectamine RNAiMax (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2019Quote: ... was used to transfect exponentially grown Raji cells with 50 nM of siHLA-DRA or siRNA universal negative control (Sigma-Aldrich, UK; SIC001) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: The custom constructs for sh-TBX2 and its non-targeting scrambled RNA duplex siRNA control (NTSCR) was procured from sigma Aldrich (RNAi single clones, Millipore sigma, Burlington, MA). These lentiviral constructs along with packaging plasmid (pCMV delta R8.2 dvpr ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with siRNAs/plasmid as described earlier and RNA was extracted using GenElute™ Mammalian Total RNA Miniprep Kit (Sigma Aldrich, # RTN70). RNA was reverse transcribe using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher #4368814) ...
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bioRxiv - Microbiology 2021Quote: ... ACOT2 and ACO7 loss of function was conducted by transfecting Huh7 cells with pooled siRNAs (Horizon Discovery/Dharmacon, Sigma-Aldrich – see Supplementary Table 1) as described previously (23 ...
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bioRxiv - Biochemistry 2022Quote: Depletion of endogenous Spindly was achieved through reverse transfection with 50 nM Spindly siRNA (5′-GAAAGGGUCUCAAACUGAA-3′ obtained from Sigma-Aldrich, (Gassmann et al., 2010)) for 48 hours with RNAiMAX (Invitrogen ...