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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for Protein FAM124A FAM124A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... HMCES-3flag protein was detected by immunoblotting with anti-flag antibody (1:10,000 dilution in PBST; Sigma, F1804-200UG) and rat anti-mouse secondary antibody peroxidase conjugate (1:20,000 dilution in PBST ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were purified using Amicon Pro Affinity Concentration Kit Protein A with 10kDa Amicon Ultra-0.5 Device (Millipore-Sigma). Antibody concentrations were determined using an IgG ELISA ...
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bioRxiv - Molecular Biology 2023Quote: ... and the eluted proteins were subjected to Western Blot analysis using a GST antibody (1:1000, Millipore 05-782).
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bioRxiv - Immunology 2023Quote: ... Proteins were subsequently transferred onto nitrocellulose membranes and immunoblot analysis was performed with following antibodies: HA (Sigma-Aldrich, H6908), FLAG (M2 ...
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bioRxiv - Microbiology 2023Quote: ... 4µg of the appropriate antibody and 20µl of Magnetic protein A/G beads (Magnachip, EMD-Millipore, Billerica, MA, USA) were used for the IP step ...
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bioRxiv - Molecular Biology 2023Quote: The following commercially available antibodies were used to detect targeted proteins: anti-RBM17 (1:1500 dilution; HPA037478, Sigma-Aldrich), anti-SF3B1 (1:3000 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... 3FLAG-6HIS-YFP-tagged proteins expressed from the endogenous locus were immunoprecipitated using anti-FLAG M2 antibodies (F3165, Sigma) (Unnikrishnan et al. ...
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bioRxiv - Genetics 2024Quote: ... 1mg of cell lysate was incubated with blocked Protein G beads and monoclonal L1 ORF1p antibody (EMD Millipore, MABC1152) at 12 rpm at 4°C overnight ...
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bioRxiv - Plant Biology 2024Quote: ... His- and GST-tagged proteins were detected with anti-His (Monoclonal Anti-polyHistidine antibody, Sigma H1029, 1:5000 dilution) or anti-GST (Mouse anti-GST monoclonal antibody ...
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bioRxiv - Biochemistry 2024Quote: ... and expression of the GFP-tagged protein was verified by western blot using an anti-GFP antibody (Sigma-Aldrich). Colonies with the highest expression for a given construct were used to inoculate 300 mL of YEPD media containing 200 µg/mL Zeocin and grown overnight with shaking (220 rpm ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in cell lysates using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000). HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908 ...
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bioRxiv - Molecular Biology 2023Quote: ... The various 3xF tagged NRPD1 and CLSY proteins were detected using a monoclonal anti-FLAG-HRP antibody (Sigma #A8592). RDR2-eGFP was detected using an anti-eGFP polyclonal antibody68 ...
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bioRxiv - Neuroscience 2024Quote: ... to perform immunohistochemical procedures in the Penn Digital Neuropathology Lab as described previously.21,61 Immunohistochemistry for neuronal markers included antigen retrieval using citrate buffer and heat for neuronal nuclei protein (NeuN) enriched in most neurons (Anti-NeuN mouse monoclonal antibody, clone A60,1:1000, Millipore) and heavy non-phosphorylated neurofilament (NF-H ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the equivalent of 500-800 µg of protein was incubated with 1 µg of anti-GFP antibody (Sigma-Aldrich) and then incubated with Protein A/G magnetic beads (Thermofisher ...
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bioRxiv - Cell Biology 2019Quote: ... and proteins precipitated using protein-A Agarose (Sigma-Aldrich) prior to analyses by Western blotting.
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bioRxiv - Microbiology 2022Quote: ... proteins proteins were electrophoretically transferred to nitrocellulose membranes (Millipore) using a Transblot semi-dry transfer cell (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 μl of Protein G magnetic beads were used and 10 μg of mouse monoclonal anti-FLAG primary antibody M2 (Sigma) were added before overnight incubation at 4°C with the lysate ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were analysed by standard Western blotting procedures using an HRP conjugated secondary antibody and Immobilon chemiluminescence substrate (Millipore-Sigma). Membranes were imaged on an Amesham Imager 600 (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were analysed by standard Western blotting procedures using an HRP conjugated secondary antibody and Immobilon chemiluminescence substrate (Millipore-Sigma). Membranes were imaged on an Amesham Imager 600 (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 µg of protein was incubated overnight at 4°C with primary antibody as indicated (anti-AHCY [MBL RN126PW], anti-HA [Millipore 05-904] ...
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bioRxiv - Cell Biology 2022Quote: ... Immunohistochemistry was used to detect protein expression using the following antibodies on paraffin sections: SftpC (rabbit, Millipore, AB37086, 1:250), and Ki67 (eBioscience ...
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bioRxiv - Microbiology 2019Quote: ... The proteins were transferred to a PVDF membrane followed by incubation with Anti-Cas9 antibody conjugated to FITC (Sigma Aldrich). Anti-mouse secondary antibody conjugated to Alkaline phosphatase was used to develop the blot with NBT-BCIP (nitro-blue tetrazolium and 5-bromo-4-chloro-3’-indolyphosphate).
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with antibodies (5-8 μg) overnight at 4°C followed by incubation for 3 hours with protein G-sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 3 h with protein G Sepharose beads (Millipore). Normal mouse ...
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bioRxiv - Microbiology 2020Quote: ... and equal amounts of protein were analyzed using the following antibodies: anti-FLAG M2 (Sigma, cat#F3165, RRID:AB_259529; 1:7,500), anti-IE1 (clone 1B12 (Zhu et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts were incubated overnight with 1 μg of anti-PDGFRβ (D-6) antibody and immunocomplexes recovered using 25 µL Protein G Sepharose beads (Sigma) for 1 h 30 min in the wheel at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mg of proteins was used to performed incubation overnight at 4°C with 5 μg of anti-Flag mouse antibody (Sigma) in 250 μL of complemented non-denaturing lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were sequentially incubated with primary and HRP-coupled secondary antibodies and proteins were detected using chemiluminescence reagents (Luminata Crescendo, Millipore) and X-ray films ...
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bioRxiv - Microbiology 2021Quote: ... nsp8 and nsp12 proteins were subsequently detected using the mouse monoclonal anti-DYKDDDDK M2 antibody (Catalogue number F3165, Sigma-Aldrich) diluted 1:1000 in TBST/5% milk ...
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bioRxiv - Cell Biology 2021Quote: ... 18 to 24 hrs after transfection the cells were lysed and incubated with CycB1 antibody overnight at 4°C and Protein G Sepharose beads (Sigma) were added to each sample for 3.5 hours at 4°C after the overnight incubation with antibody ...
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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). For PDAC cells grown as 3D organoids in co-culture ...
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bioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). Organoids were grown in complete media when passaging ...
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bioRxiv - Plant Biology 2021Quote: ... Chromatin samples were incubated with 50 ml of washed Protein G Dynabeads pre-coated with 1mg of S9.6 antibody (Millipore MABE1095) for 16h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (36) ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was assessed 72 hours post transfection via Western Blot using a monoclonal antibody against the FLAG epitope (Sigma) (Figure 5A ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200), parasite cyst wall and parasite cytoplasm by in-house mouse SalmonE anti-CST1 (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Serial dilutions of mouse sera were tested for binding to the biotinylated proteins and the bound antibodies detected using HRP-conjugated anti-mouse IgG A4416 (Sigma) and TMB substrate (Life Technologies).
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated by 12% SDS–PAGE gels and detected using immunoblotting with a monoclonal α-Flag antibody (Sigma-Aldrich) or a α-GFP antibody (Invitrogen) ...
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bioRxiv - Neuroscience 2019Quote: ... lysates of tissues or tells were incubated with antibodies overnight (4°C) and subsequently with protein G-agarose beads (Millipore) for 5h (4 °C) ...
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bioRxiv - Plant Biology 2021Quote: ... Wild-type and mutated Pik-1 and Pik-2 proteins were detected probing the membrane with anti-FLAG M2 antibody (Sigma) and anti-HA high affinity antibody 3F10 (Roche) ...
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bioRxiv - Biochemistry 2022Quote: ... recombinant proteins were purified by anti-c-Myc Antibody Beads (10D11; Wako) or Anti-FLAG M2 affinity gel (Sigma-Aldrich) following the standard protocol ...
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bioRxiv - Cell Biology 2022Quote: ... β-actin protein level was evaluated by western blot analysis of rehybridized membranes with anti-β-actin antibody (Sigma, A5441) at a 1:5000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... After blocking samples were incubated for 120 hours with primary antibody or trimeric RBD protein in PBSGT with 0.1% saponin (S2149, Sigma-Aldrich) on a shaking incubator at 200 rpm ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 μl of Protein G coated Bio-Adembeads (Ademtech) were incubated with either 1 μg of mouse monoclonal anti-FLAG antibody (F3165, Sigma), or goat anti-HA antibody as mock (sc-805 ...
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bioRxiv - Cell Biology 2022Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (Chatterjee et al. ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... For capture of antibodies and Fc-Fusions a CM5-S-Series sensor chip (Cytiva) was functionalized with 2500RU recombinant Protein A (Sigma) using EDC/NHS Coupling Kit (Biacore/Cytiva BR-1000-50 ...
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bioRxiv - Molecular Biology 2019Quote: ... Sonicated lysates were pelleted for 5 min at 14000 rpm in a microcentrifuge and the entire supernatant was transferred to a new microfuge tube and incubated overnight at 4°C with 5 µg of anti-Myc antibody (9E10) and 20 µl of protein G magnetic beads (Millipore). Following IP ...
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bioRxiv - Microbiology 2019Quote: ... Bound proteins were separated by SDS-PAGE and transferred to nitrocellulose before incubation with an anti-myc antibody (9E10; Sigma). Blots were revealed with the Odyssey Western Detection System.
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bioRxiv - Cell Biology 2019Quote: ... antibodies were added for 1 hour at room temperature and protein signal was then visualized using Immobilon Forte (Millipore, WBLUF0500) on ImageQuant LAS 4000 (GE Healthcare Life Sciences).