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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... and contaminated DNA was removed according to On-column DNase digestion protocol (Sigma, USA). Total RNA was quantified with a UV spectrophotometer and quality of RNA was assessed by gel-electrophoresis ...
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bioRxiv - Immunology 2022Quote: ... Minced tissue was then subjected to rounds of digestion in RPMI 1640 medium (Sigma) containing 5% FBS (Life Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... The digestion was terminated by α-MEM media with 10% HSA (Merck Millipore, 823022). After digestion ...
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bioRxiv - Immunology 2023Quote: ... leukocytes were isolated from whole brains after digestion in 0.05% collagenase A (Sigma-Aldrich) and 10 mg/ml DNase I (Sigma-Aldrich) ...
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bioRxiv - Pathology 2023Quote: ... pepsin digestion was performed using 2 mL of 2 mg/mL pepsin (Sigma-Aldrich) solution in 0.01 M HCl for 40 mg dry ECM powder in a 7.5 mL glass vial as previously described [25] ...
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bioRxiv - Microbiology 2023Quote: ... then the sample was incubated in digestion buffer (0.8 M guanidine-HCl (Sigma, G3272), 50 mM Tris·HCl pH 8 ...
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bioRxiv - Immunology 2023Quote: ... The tissue was minced prior to digestion in supplemented RPMI (Collagenase Type VIII (Sigma), DNAse I 10U/mL (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... On-bead digestion was performed with 1 µg of trypsin (Sigma Aldrich, Cat# 6567) in 70 µl of ABC buffer ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was immediately extracted from pooled samples after grinding using a Spectrum™ Plant Total RNA Kit from Sigma-Aldrich (STRN250) and an On-Column DNase I Digestion Set from Sigma-Aldrich (DNASE70-1SET) to remove DNA ...
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bioRxiv - Microbiology 2023Quote: ... mouse lungs were inflated with 0.7 mL of digestion mix containing collagenase A (Sigma), dispase (Thermofisher) ...
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bioRxiv - Cell Biology 2023Quote: ... The digestion buffer additionally contained 0.2 mg/mL protease type XIV (P5147, Sigma-Aldrich), which may further help dissociate cell clusters into single cardiomyocytes ...
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bioRxiv - Plant Biology 2023Quote: ... following the manufacturer instructions and including an on-column DNase I digestion step (Sigma). First strand cDNA was synthesized with Superscript III or Superscript IV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Genomics 2023Quote: ... 20 mM 2-mercaptoethanol) by digestion with ∼19,000 units of lyticase (Sigma L-2524) at 30°C for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... transfer it to a centrifuge tube and add digestion fluid (collagenase II(Sigma, USA)) ...
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bioRxiv - Cancer Biology 2024Quote: ... The resultant fragments underwent digestion with a mix of DNase I (10104159001, Sigma, USA) and Collagenase D (11088858001 ...
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bioRxiv - Cell Biology 2023Quote: ... The separation of the hydrophobic and hydrophilic proteins from the pellet was carried out with CelLyticTM MEM Protein Extraction Kit (Sigma-Aldrich, MO, USA) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: PLA was performed to detect protein-protein interactions in MDA-MB-231 cells using Duolink™ In Situ Red Starter Kit (Sigma, cat. DUO92101). All the reagents ...
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
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bioRxiv - Physiology 2022Quote: ... Total protein concentration in the samples was determined with a standard BSA kit (Millipore). 15 µL of the sample was diluted with 60 µL ultrapure water in order to keep the concentration within the linear range of the calibration curve (0.2 to 2 µg⋅µL−1) ...
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bioRxiv - Systems Biology 2020Quote: ... Total protein concentration was measured using bicinchoninic acid assay (BCA Kit, Sigma-Aldrich, USA).
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bioRxiv - Cell Biology 2021Quote: ... was conjugated with Atto-647N using a protein labeling kit (cat # 76508 Sigma-Aldrich). PDMS stamps were incubated with solution containing 50μg/ml fibronectin and 1μg/ml of the conjugated fibronectin in Dulbecco’s phosphate-buffered saline (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: RIP assays employed the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: RIP assay was done with Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA). Quantitative RT-PCR was used for determining total RNA levels.
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was estimated with the Quantipro BCA assay kit (Sigma-Alrich, United States), and lysates were resolved on a 10% sodium dodecyl sulfate polyacrylamide gel prior to transfer onto polyvinylidene fluoride membranes ...
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bioRxiv - Cancer Biology 2020Quote: ... and membrane fractions were isolated using a Compartment Protein Extraction Kit (Millipore, Billerica, MA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... Soluble protein was measured using a Bicinchronicinc acid kit (BCA1, Sigma-Aldrich Canada Co.). Absorbance of each reaction was measured in a spectrophotometer (Spectra Max M2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were detected with an enhanced chemiluminescence assay kit (ECL Plus, Millipore Corporation, USA). Signals were visualized using ImageLab 1.46 (BioRad ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich, Pool, UK) using bovine serum albumin as standard ...
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bioRxiv - Molecular Biology 2022Quote: ... Total or nuclear protein was extracted using a nuclei isolation kit (NUC201, Sigma-Aldrich). Protein samples were electrophoresed on a 10% sodium dodecyl sulfate-polyacrylamide gel and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Cancer Biology 2023Quote: ... secondary antibodies were added and the proteins were detected using an ECL kit (Millipore). Immunoreactive signals were visualized using the GE Amersham Imager 600 chemiluminescence system.
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bioRxiv - Microbiology 2023Quote: ... Protein precipitates were solubilized in 6M urea and subsequently quantified using BCA kit (Sigma). Ten micrograms of the protein samples were reduced with 10mM dithiothreitol (DTT ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using the Pierce™ BCA assay kit (Sigma-Aldrich, Germany).
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bioRxiv - Plant Biology 2021Quote: ... Digestion was stopped by addition of 20 μL 20 mM ammonium formate (AF, Sigma-Aldrich). The sample was centrifuged for 20 min at 16.000 x g and the peptides in the supernatant were purified.
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bioRxiv - Developmental Biology 2021Quote: ... NaCl 0,15M and diluted 5-fold prior digestion with 80ug of trypsin (TPCK-treated, Sigma) overnight at 37° C ...
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bioRxiv - Neuroscience 2021Quote: ... and Papain digestion was blocked adding FACS buffer 0.02% Chicken egg white inhibitor (Sigma, #T9253). The cell suspension was passed through a 70 µm strainer (ClearLine ...
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bioRxiv - Bioengineering 2019Quote: ... The digestion buffer was prepared by mixing: Triton X-100 (Sigma Aldrich, catalog no. 93426) to a final concentration of 0.5% (w/v ...
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bioRxiv - Immunology 2019Quote: Brains were minced in a digestion solution containing 1 mg/mL type II collagenase (Sigma) and 1% DNAse (Sigma ...
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bioRxiv - Genetics 2019Quote: ... testis tissue was incubated in digestion solution 1: Hanks’ Balanced Salt solution (HBSS, Sigma Aldrich), 1 mg/ml collagenase Type I (Worthington Biochemical) ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by Proteinase K digestion (5 μl of 20 mg/mL; Sigma-Aldrich; Cat#: P2308) during 2 hours ...
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bioRxiv - Genomics 2021Quote: ... During digestion we treated the tissue with 250 unit/µL of Benzonase (Millipore, 71206-3) to prevent neuronal clumping due to genomic DNA released from dead cells ...
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bioRxiv - Physiology 2021Quote: Pancreatic islets were isolated by enzymatic digestion using collagenase type V from Clostridium histolyticum (Sigma), dissolved in Hanks’ Solution (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... then digestion was terminated by addition of 200ul 0.1% trypsin inhibitor solution (Sigma-Aldrich, USA), cells were resuspended in 1ml PBS and then pelleted by centrifugation at 400 x g for 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA from the micro-dissected cells were extracted through enzymatic digestion with proteinase K (Sigma), then reverse transcribed with 5’-biotin modified oligo(dT)24 (IDT ...
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bioRxiv - Developmental Biology 2022Quote: ... and this was followed by digestion with a protease (Cat# P5147, Sigma; 1 mg/mL) and dispase (Cat# LS02104 ...
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bioRxiv - Cancer Biology 2020Quote: ... Mechanical dissociation was followed by enzymatic digestion in 435U/mL deoxyribonuclease I (Sigma-Aldrich, D5025) and 218U/mL collagenase (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Digestion buffer for immune cell analysis additionally included type IV collagenase (2.5 mg/ml, Sigma). Digested tissues were passed through a 70-µm cell strainer and washed once with 40 ml PBS containing 5% FBS and treated with red blood cell lysing buffer (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... Peptides from both consecutive digestions were recovered by solid phase extraction using C18 ZipTips (Millipore), eluted in 2x7 μl aliquots of 50% MeCN 0.1% formic acid ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides from both consecutive digestions were recovered by solid phase extraction using C18 ZipTips (Millipore), and resuspended in 0.1% formic acid for analysis by LC-MS/MS ...
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bioRxiv - Cell Biology 2021Quote: Single cell dissociation was performed through enzymatic digestion with 600 U ml−1 collagenase (Sigma) and 200 U ml−1 hyaluronidase (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... tissue was incubated in digestion buffer containing 10 mg/ml Bacillus Licheniformis enzyme (Sigma, P5380) for 20 min ...