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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... was added into 200 µL cell lysate precleared by 20 µL Protein A magnetic beads (EMD Millipore) and incubated overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... the immunocomplexes were collected with 25 μL of PureProteome™ Protein A Magnetic (PAM) Beads (Millipore, LSKMAGA10) for 2 hours at 4°C with rotation.
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bioRxiv - Biochemistry 2023Quote: ... The solubilized proteins were mixed with 20 µL (40 µL slurry) anti-Flag M2 magnetic beads (Sigma) and rolled for 3 hours at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... 25 μl of bead slurry (Anti-Flag M2 Magnetic Beads; Sigma-Aldrich) was washed twice with 1 ml of ice-cold wash buffer (100 mM KCl ...
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bioRxiv - Genetics 2023Quote: ... 400 µL of the supernatant was transferred to 1 mL deep-well 96 well plates loaded with 500 µL of GNB magnetic beads (GNB magnetic bead recipe: 10 mL Sera-Mag caroboxylate-modified SpeedBeads (Sigma) washed 3x in 10 mL of 1xTE ...
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bioRxiv - Microbiology 2023Quote: ... The cellular extract was centrifuged (10000 x g for 20 min at 4 °C) and incubated with anti-FLAG M2 magnetic beads (Sigma) for 2h at 4 °C following washing and elution steps using a magnetic separation stand according to the manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... anti-FLAG® M2 Magnetic Beads (Sigma-Aldrich), anti-TCHP (abcam) ...
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bioRxiv - Developmental Biology 2021Quote: ... Anti-Flag M2 magnetic beads (Sigma-Aldrich, M8823) were used to immunopurify eF-Piwi from OSCeF-Piwi (https://doi.org/10.1101/2020.12.18.423517) ...
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bioRxiv - Molecular Biology 2020Quote: ... Anti-FLAG M2 antibody coupled magnetic beads (Sigma) were washed x3 with IP buffer before incubation with the precleared lysate overnight rotating at 4oC ...
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bioRxiv - Microbiology 2020Quote: ... except that anti-FLAG magnetic beads (Sigma Aldrich) were used to pull-down FLAG-tagged proteins and any associated proteins ...
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bioRxiv - Neuroscience 2019Quote: ... or anti-FLAG M2 magnetic beads (Sigma M8823) for 3-4h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl streptavidin-magnetic beads (ThermoFisher or Millipore) were used for 1.0×108 cells ...
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bioRxiv - Neuroscience 2020Quote: ... Beads were isolated using a magnetic stand (Millipore) and washed 3 × 10 min with RIPA buffer (20mM tris-hcl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... Anti-FLAG M2 Magnetic Beads (Sigma Aldrich, M8823) were pre-equilibrated with 20 mM Tris/Cl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Anti-FLAG M2 Magnetic Beads (Sigma Aldrich, M8823) were washed and pre-equilibrated with ice-cold 20mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... 500 μL M2 magnetic FLAG-beads (SIGMA, M8823) were added to the supernatant and incubated for 120 min rotating at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... 50 μl of anti-FLAG magnetic beads (Sigma) were then added into the lysates for overnight incubation on a rotator at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... or magnetic a-FLAG M2 beads (Sigma-Aldrich) and incubated at 4°C overnight ...
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bioRxiv - Plant Biology 2020Quote: ... 400µL of M2 magnetic FLAG-beads (Sigma, M8823) were added to the supernatants ...
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bioRxiv - Molecular Biology 2019Quote: ... or 20μL of FLAG (M2) Magnetic Beads (Sigma) were added to the cell lysates and were left to rotate overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... anti-FLAG® M2 magnetic beads (Sigma-Aldrich) for FLAG-EDS1 ...
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bioRxiv - Genetics 2020Quote: ... Glutathione High Capacity Magnetic Agarose Beads (Sigma G0924) were added and the mixture was rotated 1 hour at RT ...
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bioRxiv - Cell Biology 2021Quote: ... 40 μl anti-FLAG M2 magnetic beads (Sigma) were added to each nuclear extract and incubated 6 hours at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-FLAG® M2 magnetic beads (Sigma) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... or Anti-FLAG M2 Magnetic beads (Sigma-Aldrich), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... anti-FLAG M2 magnetic bead (Sigma Aldrich M8823); goat anti-rabbit secondary antibody (Biotium 20064) ...
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bioRxiv - Molecular Biology 2022Quote: ... except that anti-Flag M2 magnetic beads (Sigma) were used ...
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bioRxiv - Genetics 2023Quote: ... Anti-FLAG M2 magnetic beads (Sigma-Aldrich, M8823) were used to immunoprecipitate CRTC-1::3xFLAG ...
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bioRxiv - Neuroscience 2023Quote: ... TNFα) (Millipore HCYTA-60K Luminex magnetic bead panel); Angiogenesis (angiopoietin-2 ...
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bioRxiv - Biochemistry 2023Quote: ... or Anti-FLAG M2 magnetic beads (Sigma, M8823), depending on the target protein ...
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bioRxiv - Biochemistry 2023Quote: ... Anti-Flag M2 magnetic beads (Sigma-Aldrich, #M8823) were added to the supernatant ...
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bioRxiv - Cell Biology 2024Quote: ... anti-Flag magnetic beads (Sigma-Aldrich, cat # M8823) were washed using 1 × TBS buffer (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... IPs were done with monoclonal anti-HA antibodies coupled beads (Sigma) or anti-FLAG antibodies and protein A/G beads (SantaCruz ...
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bioRxiv - Cell Biology 2020Quote: ... cell lysates (750 μg total protein) were precleared with empty agarose beads and then incubated with anti-FLAG-M2 agarose affinity beads (Sigma) with rotation overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Negative control IPs were performed using IgG coupled beads (Sigma-Aldrich; 5 μg antibody/mg beads). Approximately 10 μg of IP lysate were used for immunoblotting as described above ...
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bioRxiv - Biophysics 2022Quote: ... Protein G (Sigma) was coupled at 10 µg/mL in the presence of 10 mM sodium acetate buffer pH 4.5 at 30 µL/min for 300 seconds until ∼3500-4000 RU was immobilized ...
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bioRxiv - Microbiology 2021Quote: ... Protein G (Sigma) was covalently coupled following activation ...
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bioRxiv - Biophysics 2020Quote: ... Protein G (Sigma) at 10 μg/mL was coupled in the presence of 10mM sodium acetate buffer pH 4.5 at 30 μl/min for 300 seconds in various channels ...
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bioRxiv - Cancer Biology 2019Quote: ... A preclearing step was made for 2hours at 4°C with 50µl of previously blocked protein-A and protein-G beads (Sigma) for 200µg of chromatin ...
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bioRxiv - Cell Biology 2021Quote: ... and protein diluted to 1mg/ml 1ml of protein lysate was precleared with 20μl of protein G sepharose bead slurry (Sigma) for 1 hour to remove native immunoglobulins ...
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bioRxiv - Microbiology 2023Quote: ... For p65 IP the lysates were then incubated with 2 μg of antibody CST ref #6956 or CST ref# 8242) at 4 °C for 2 hrs before adding 20 μL of slurry protein G beads (Sigma-Aldrich Fast Flow Protein G sepharose) for 20 min ...
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bioRxiv - Genomics 2020Quote: ... Approximately 10 mg of total protein was added to 30 μl anti-FLAG M2 magnetic beads (Sigma # M8823) prepared according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The Ni-NTA eluted proteins were incubated with washed/pre-conjugated anti-FLAG magnetic beads (Millipore™ M8823) overnight ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... Identical amounts of crude extracts were incubated with prebound @AGO1 (5μg) PureProteome Protein A magnetic beads (30μL; Millipore) for 2 hours at 7 rpm in the cold room ...
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bioRxiv - Microbiology 2021Quote: ... 1% Triton 100 and 1 × protein inhibitor) and subsequently precipitated with anti-Flag M2 Magnetic beads (Sigma, M8823) or GFP-Trap (Chromotek ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mg of protein lysate was incubated overnight with 30 μL Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich) at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-FLAG M2 Magnetic Beads (20 µL bead suspension per reaction, Sigma, #M8823) or GFP/RFP-Trap Magnetic Agarose beads (15 µL bead suspension per reaction ...
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bioRxiv - Microbiology 2019Quote: ... Protein A/G Plus agarose beads were blocked with bovine t-RNA (1mg/mL; Sigma) for 2h in modified-RIPA buffer ...
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bioRxiv - Microbiology 2019Quote: ... incubated with mouse α-anti-HA clone 12CA5 conjugated with protein G beads (Sigma-Aldrich) for 16 hours at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... The remainder of the samples was incubated for 1hr with uncoated protein G beads (Millipore) to remove a-specifically bound proteins ...