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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 5 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... whole cell Triton X-100 lysates were incubated with Flag-M2 agarose (Sigma) and washed with lysis buffer three times ...
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bioRxiv - Plant Biology 2022Quote: ... Whole leaves were immersed in octadecafluor-decahydronaphthalin (Sigma-Aldrich, Littlejohn et al., 2010). The samples were analyzed using a Leica True Confocal Scanner SP8 microscope equipped with an HC PL APO CS2 40 × 1.10 water immersion objective (Leica ...
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bioRxiv - Molecular Biology 2022Quote: ... whole-lung suspension was treated with Red Blood Cell Lysis Buffer (Millipore Sigma) for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... Whole blood was acquired with heparin sodium (Sigma - 20 USP units per ml) and gently layered onto 2/3 volume of Histopaque 1077 in a centrifuge tube ...
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bioRxiv - Developmental Biology 2022Quote: ... Whole-Mount Immunostaining: hCOs were fixed for 60 minutes with 1% paraformaldehyde (Sigma) at 22ºC and washed 3X with PBS ...
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bioRxiv - Developmental Biology 2020Quote: Samples for whole mount in situ hybridization were treated with Proteinase K (Sigma), neutralized with 2 mg/ml glycine ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole-cell extract was prepared from 1X radioimmunoprecipitation assay lysis buffer (RIPA; Millipore). Extract was clarified by centrifugation at 15,000g for 15 min at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... The whole clarified brain was transferred to Histodenz solution at pH 7.5 (Sigma). Brains were immersed in a refractive index matching solution (RIMS ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were isolated from whole blood using Accuspin System Histopaque-1077 (Sigma-Aldrich). Institutional review board approval for studies involving human tonsils was obtained from Seattle Children’s Hospital ...
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bioRxiv - Cell Biology 2021Quote: ... goat anti-mouse whole molecule conjugated to FITC (Sigma F-2012, 1:400); goat anti-rabbit IgG (H+L ...
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bioRxiv - Immunology 2022Quote: PBMCs were isolated from whole blood samples using Histopaque®-1077 (Sigma-Aldrich) and separated according to manufacturers’ instructions ...
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bioRxiv - Immunology 2022Quote: ... HuPBMCs were isolated from whole blood by Histopaque density gradient centrifugation (Sigma-Aldrich). Cells were stained with 1.75µg/ml of VRC07-AlexaFluor647 conjugated isotypes ...
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bioRxiv - Molecular Biology 2022Quote: We prepared whole cell lysates in ice-cold cell lysis buffer (Sigma-Aldrich). We separated Protein samples on a TGX gel (Bio-Rad) ...
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bioRxiv - Developmental Biology 2023Quote: Whole limbs were mounted in 0.8% ultra-low gelling temperature agar (Sigma, A5030) in 1×PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse IgG HRP linked whole Ab (Sigma; #NA931-1ML, 1:5000; lot #: 17041904), and Rabbit IgG HRP linked whole Ab (Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... Bands were detected using the anti-rabbit IgG (whole molecule)-Peroxidase (A0545, Sigma) at 1:20000 dilution and the SuperSignal West Femto Trial Kit (Thermo Scientific).
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bioRxiv - Neuroscience 2023Quote: ... or Mouse IgG HRP Linked Whole Antibody (Millipore Sigma, Darmstadt, Germany; Cat: NXA931V). Signals were detected with enhanced chemiluminescence using Pierce ECL Blotting Substrate (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... either Rabbit IgG HRP Linked Whole Antibody (Millipore Sigma, Darmstadt, Germany; Cat: GENA934) or Mouse IgG HRP Linked Whole Antibody (Millipore Sigma ...
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bioRxiv - Physiology 2023Quote: Whole mesenteric arteries were dissected and placed into RIPA buffer (Sigma-Aldrich: R0278) containing protease inhibitor cocktail (Sigma-Aldritch P8340 ...
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bioRxiv - Developmental Biology 2023Quote: ... Whole-cell lysates were prepared using RIPA cell lysis buffer (Sigma-Aldrich, R0278) containing 1:100 phosphatase inhibitor cocktail 2 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... Pellet was used for extraction of whole RNA with Tri-Reagent (Sigma-Aldrich) according to manufacturer’s procedure ...
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bioRxiv - Cell Biology 2023Quote: ... Whole mount membranes were permeabilized with 0.1% v/v Triton-X 100 (Sigma) and blocked with 10% v/v Goat serum (Genesearch ...
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bioRxiv - Genetics 2024Quote: Fresh whole testis samples were fixed in 5ml of Bouin’s solution (Sigma HT10132) for at least 48-72 hours and then stored in 70% ethanol until further processing ...
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bioRxiv - Bioengineering 2023Quote: ... anti-Mouse IgG (whole molecule)– Peroxidase antibody produced in goat (A4416; Sigma-Aldrich) in 1:18,000 dilution ...
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bioRxiv - Immunology 2023Quote: ... whole blood was incubated with an equal volume of 3% dextran (Sigma #D1037) in 0.9% NaCl for 30min at 37°C to facilitate red blood cell sedimentation ...
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bioRxiv - Cell Biology 2024Quote: ... We used calf thymus-derived H2A histones (Sigma-Aldrich, #H9250; unfractionated whole histone), E ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 μM 5-Aza-2′-deoxycytidine (5-Aza; Sigma), 250-500 nM CpG-scramble ...
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bioRxiv - Molecular Biology 2020Quote: ... HCT116 shNT and shDHX30 cells were cultured in 96-well tissue culture plate and treated with 100mM 5-Fluorouracil (Sigma Aldrich) as positive control ...
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bioRxiv - Developmental Biology 2019Quote: ... on gelatin-coated plates and grown for 5 days in the presence/absence of L-Pro (250–500 μM) (Sigma-Aldrich), with a medium change at day 3 ...
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bioRxiv - Genomics 2021Quote: ... and sorting G1 cells based on Hoechst staining on an Influx FACS machine into 384 well plates containing 5 μl sterile filtered mineral oil (Sigma Aldrich) per well ...
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcriptase qPCR was performed in a Framestar-480/384 well plate containing 5 µl of 2x SYBR Green JumpStart Taq ReadyMix (Sigma-Aldrich), 2 µl cDNA ...
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bioRxiv - Microbiology 2020Quote: Bacterial plate counts were performed on lysogeny broth (LB-Miller) agar plates (10 g tryptone, 5 g yeast extract, 10 g NaCl, and 15 g agar (all from Sigma-Aldrich) in 1 L of Millipore water) ...
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bioRxiv - Cell Biology 2021Quote: ... single cell clones were generated by serial dilution in 96-well plates and maintained in growth media with 200 μg/mL Zeocin and 5 μM methotrexate (MTX, EMD Millipore). CHO-SEAP CMV::XRCC6 ATM+ PRKDC+ was generated analogously to CHO-K1 ATM+ PRKDC+ ...
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bioRxiv - Evolutionary Biology 2022Quote: ... bacterial densities were determined by plating out 100 µl of a dilution series ranging from 10−5 to 10−7 on Vibrio selective Thiosulfate Citrate Bile Sucrose Agar (TCBS) plates (Sigma Aldrich). Plates were incubated over night at 25° C and the total amount of colonies was counted the following day.
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bioRxiv - Immunology 2022Quote: Lysates from cells treated in 6-well plate were heated for 5 minutes at 100 ºC with the same volume of Leammli Buffer 2X (Sigma-Aldrich). Samples were separated in a vertical electrophoresis on 12% polyacrylamide gels (Biorad ...
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bioRxiv - Molecular Biology 2022Quote: ... until 10-7 and 10 µl of the dilutions between 10-5 and 10-7 were plated in BHI agar plates supplemented with Congo Red (Sigma®) at a final concentration of 25 µg.ml-1 ...
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bioRxiv - Neuroscience 2022Quote: ... UAS-CsChrimson/UAS-Aion) were grown in darkness on grape agar plates with yeast paste containing 5 mM all-trans-retinal (Sigma-Aldrich). Staged third instar larvae (96 h ± 3 h AEL ...
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bioRxiv - Microbiology 2021Quote: ... 5 µl of the reaction mixture (in duplicate) was quenched into a MultiScreenHTS Durapore 96-well filter plate (MSHVN4B50; Millipore Sigma) containing 200 µl of 10% charcoal in 0.5% HCl and 50 µl of wash buffer (1 M HCl with 200 mM NaPPi) ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus-containing supernatants were collected 48 h later and added with Polybrene to AML cells pre-seeded at ∼5 × 105/well in 24-well plates (Sigma-Aldrich). After 8 h ...
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bioRxiv - Microbiology 2022Quote: ... 5 μL was used to inoculate 200 μL of LB broth containing 5 μg/mL of tobramycin in a well of a 96-well plate (Corning, Sigma-Aldrich). An additional 5 μL was added to a corresponding well of another 96-well plate containing 200 μL of LB broth ...
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bioRxiv - Cell Biology 2022Quote: ... trypsinized cells were resuspended in standard growth medium without antibiotics and plated in 6-well culture plates (5 × 104 cells/well) after mixing with 10 µg/ml polybrene (EMD Millipore) and lentivirus supernatant ...
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bioRxiv - Genomics 2019Quote: ... at which point the fibrin gels were sufficiently polymerized around the posts to be lubricated in media and transferred from the casting troughs into a 24-well plate with fresh 3D-EHT media (RPMI-B-27 with penicillin/streptomycin, and 5 mg/mL aminocaproic acid, Sigma-Aldrich). 3D-EHTs were supplied with 2.5 mL/well of fresh 3D-EHT media three times per week.
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bioRxiv - Cell Biology 2021Quote: ... at which point the fibrin gels were sufficiently polymerized around the posts to be lubricated in media and transferred from the casting troughs into a 24-well plate with fresh 3D-EHT media (RPMI-B27 with penicillin/streptomycin, and 5 mg/ml aminocaproic acid; Sigma-Aldrich). 3D-EHTs were supplied with 2.5 ml/well of fresh 3D-EHT media three times per week.
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bioRxiv - Neuroscience 2021Quote: ... plated at a density of 2-5 × 104 cells /well on coverslips in a 24-well plate pre-coated with poly-L-lysine (Sigma, P9155) and cultured in a Neurobasal medium supplemented with 2% B27 supplement ...
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bioRxiv - Cancer Biology 2021Quote: ... GSCs used were between passages 5 and 30 and cultured either as spheres or as attached on fibronectin-coated plates (10ug/ml, Millipore Sigma) in a medium of 1X Neurobasal Medium ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed 5 times with PBS/0.5% Tween-20 and 50µl of HRP-anti-mouse-IgA (Sigma-Aldrich, 1:1000) was added to each well ...
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bioRxiv - Microbiology 2022Quote: ... A yeast colony was picked from a YSM-Trp plate and resuspended in 100 µl of 5% w/v Chelex-100 (Sigma-Aldrich). Half the sample volume of acid washed glass beads were added and the sample vortexed at maximum speed for 4 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 3 to 5 isolated morphologically normal colonies selected from the agar plates were suspended in cation adjusted Mueller Hinton broth (CAMHB, Sigma-Aldrich). The bacterial suspensions were diluted with normal saline to achieve a turbidity equivalent to a 0.5 McFarland standard (latex ...
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bioRxiv - Cell Biology 2023Quote: ... cells were serially diluted 10-fold and 2 μl of each dilution was spotted on plates containing 5 mM hydroxyurea (Sigma-Aldrich) or on YEPD and exposed to UV with a dose of 200 J/m2 ...
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bioRxiv - Neuroscience 2023Quote: ... and seeded on new plates with density 1:5 – 1:10 in iPS medium with ROCK inhibitor Y-27632 (Sigma-Aldrich). Before freezing the cells were incubated with ROCK inhibitor Y-27632 (Sigma-Aldrich ...