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Citations for Millipore Sigma :
601 - 650 of 10000+ citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 5 Strip Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... single cell clones were generated by serial dilution in 96-well plates and maintained in growth media with 200 μg/mL Zeocin and 5 μM methotrexate (MTX, EMD Millipore). CHO-SEAP CMV::XRCC6 ATM+ PRKDC+ was generated analogously to CHO-K1 ATM+ PRKDC+ ...
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bioRxiv - Evolutionary Biology 2022Quote: ... bacterial densities were determined by plating out 100 µl of a dilution series ranging from 10−5 to 10−7 on Vibrio selective Thiosulfate Citrate Bile Sucrose Agar (TCBS) plates (Sigma Aldrich). Plates were incubated over night at 25° C and the total amount of colonies was counted the following day.
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bioRxiv - Immunology 2022Quote: Lysates from cells treated in 6-well plate were heated for 5 minutes at 100 ºC with the same volume of Leammli Buffer 2X (Sigma-Aldrich). Samples were separated in a vertical electrophoresis on 12% polyacrylamide gels (Biorad ...
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bioRxiv - Molecular Biology 2022Quote: ... until 10-7 and 10 µl of the dilutions between 10-5 and 10-7 were plated in BHI agar plates supplemented with Congo Red (Sigma®) at a final concentration of 25 µg.ml-1 ...
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bioRxiv - Neuroscience 2022Quote: ... UAS-CsChrimson/UAS-Aion) were grown in darkness on grape agar plates with yeast paste containing 5 mM all-trans-retinal (Sigma-Aldrich). Staged third instar larvae (96 h ± 3 h AEL ...
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bioRxiv - Microbiology 2021Quote: ... 5 µl of the reaction mixture (in duplicate) was quenched into a MultiScreenHTS Durapore 96-well filter plate (MSHVN4B50; Millipore Sigma) containing 200 µl of 10% charcoal in 0.5% HCl and 50 µl of wash buffer (1 M HCl with 200 mM NaPPi) ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus-containing supernatants were collected 48 h later and added with Polybrene to AML cells pre-seeded at ∼5 × 105/well in 24-well plates (Sigma-Aldrich). After 8 h ...
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bioRxiv - Microbiology 2022Quote: ... 5 μL was used to inoculate 200 μL of LB broth containing 5 μg/mL of tobramycin in a well of a 96-well plate (Corning, Sigma-Aldrich). An additional 5 μL was added to a corresponding well of another 96-well plate containing 200 μL of LB broth ...
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bioRxiv - Cell Biology 2022Quote: ... trypsinized cells were resuspended in standard growth medium without antibiotics and plated in 6-well culture plates (5 × 104 cells/well) after mixing with 10 µg/ml polybrene (EMD Millipore) and lentivirus supernatant ...
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bioRxiv - Genomics 2019Quote: ... at which point the fibrin gels were sufficiently polymerized around the posts to be lubricated in media and transferred from the casting troughs into a 24-well plate with fresh 3D-EHT media (RPMI-B-27 with penicillin/streptomycin, and 5 mg/mL aminocaproic acid, Sigma-Aldrich). 3D-EHTs were supplied with 2.5 mL/well of fresh 3D-EHT media three times per week.
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bioRxiv - Cell Biology 2021Quote: ... at which point the fibrin gels were sufficiently polymerized around the posts to be lubricated in media and transferred from the casting troughs into a 24-well plate with fresh 3D-EHT media (RPMI-B27 with penicillin/streptomycin, and 5 mg/ml aminocaproic acid; Sigma-Aldrich). 3D-EHTs were supplied with 2.5 ml/well of fresh 3D-EHT media three times per week.
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bioRxiv - Neuroscience 2021Quote: ... plated at a density of 2-5 × 104 cells /well on coverslips in a 24-well plate pre-coated with poly-L-lysine (Sigma, P9155) and cultured in a Neurobasal medium supplemented with 2% B27 supplement ...
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bioRxiv - Cancer Biology 2021Quote: ... GSCs used were between passages 5 and 30 and cultured either as spheres or as attached on fibronectin-coated plates (10ug/ml, Millipore Sigma) in a medium of 1X Neurobasal Medium ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed 5 times with PBS/0.5% Tween-20 and 50µl of HRP-anti-mouse-IgA (Sigma-Aldrich, 1:1000) was added to each well ...
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bioRxiv - Microbiology 2022Quote: ... A yeast colony was picked from a YSM-Trp plate and resuspended in 100 µl of 5% w/v Chelex-100 (Sigma-Aldrich). Half the sample volume of acid washed glass beads were added and the sample vortexed at maximum speed for 4 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 3 to 5 isolated morphologically normal colonies selected from the agar plates were suspended in cation adjusted Mueller Hinton broth (CAMHB, Sigma-Aldrich). The bacterial suspensions were diluted with normal saline to achieve a turbidity equivalent to a 0.5 McFarland standard (latex ...
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bioRxiv - Cell Biology 2023Quote: ... cells were serially diluted 10-fold and 2 μl of each dilution was spotted on plates containing 5 mM hydroxyurea (Sigma-Aldrich) or on YEPD and exposed to UV with a dose of 200 J/m2 ...
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bioRxiv - Neuroscience 2023Quote: ... and seeded on new plates with density 1:5 – 1:10 in iPS medium with ROCK inhibitor Y-27632 (Sigma-Aldrich). Before freezing the cells were incubated with ROCK inhibitor Y-27632 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Βecton Dickinson Company) broth or agar plates or TY medium (48) in the presence of 1-5 µg/mL thiamphenicol (Sigma-Aldrich) or anhydrotetracycline (ATc ...
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bioRxiv - Neuroscience 2023Quote: ... and neurons plated in NPM onto 24-well plates (5 x 104 cells/well) precoated with poly-L-lysine hydrobromide (Sigma P2636) (1 mg/mL for glass coverslips or 0.1 mg/mL for plasticware ...
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bioRxiv - Microbiology 2023Quote: ... and eluted directly onto the MALDI plate using 1 μL of 5 mg/mL alpha-cyano-4-hydroxycinnamic acid (CHCA, Sigma-Aldrich) in 50% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... 200 μL of cells were sonicated for 5 s before being loaded into a CellASIC® ONIX2 Y04C microfluidic plate (Y04C, Millipore) connected to the ONIX2 (Millipore ...
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bioRxiv - Systems Biology 2023Quote: ... single cells were sorted based on their GFP signal into 384-well plates containing 100 nl of unique CEL-seq2 compatible primer (7 ng/µl) and 5 µl mineral oil (Sigma-Aldrich). Following cell lysis and denaturation ...
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bioRxiv - Cancer Biology 2023Quote: ... GSCs used were between passages 5 and 30 and cultured either as spheres or as attached on fibronectin-coated plates (10 ug/ml, Millipore Sigma) in a medium of 1X Neurobasal Medium ...
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bioRxiv - Immunology 2023Quote: ... The capture matrix was washed three times with PBS and incubated over night with 1:5 diluted plasma samples in a low protein binding MultiScreen® filter plate (Millipore). Depleted plasma was recovered by centrifugation of the filter plate ...
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bioRxiv - Microbiology 2023Quote: ... from a freshly streaked out LB agar plate was inoculated into 5 mL filter-sterilised lysogeny broth (LB; L3022, Sigma-Aldrich) with ampicillin (100 µg.mL−1 ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then centrifuged at 200 rcf for 5 min and resuspended at a density of 2×105 cells/ml in ultra-low attachment 6-well plates (Sigma, CLS3471) using N2B27 medium (50% Advanced DMEM/F12 (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... 48-well MEA plates (Axion, M768-tMEA-48B-5) were first coated with 0.1 mg/ml poly-D-lysine (Sigma-Aldrich, P7886) and incubated in 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Two hundred thousand cells per well were seeded into a 12-well plate pre-coated with poly-D-lysine (5 μg/ml) in 0.1 M borate buffer (Sigma-Aldrich, #P7280). Cultures were grown in an incubator at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: ... GSCs used were between passages 5 and 30 and cultured either as spheres or as attached on fibronectin-coated plates (10 mg/mL, Millipore Sigma) in a medium of 1X Neurobasal Medium ...
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bioRxiv - Microbiology 2019Quote: ... multi-tube mixer for 3 min before adding one volume of phenol:chloroform:isoamyl alcohol (25:24:1) (Sigma-Aldrich). Samples were mixed vigorously ...
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bioRxiv - Microbiology 2021Quote: ... Plates sealed with a Breathe Easy plate seals (Sigma) and aerated by micro-orbital shaking in an ELTI plate shaker at 37 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Their relative expression was monitored by ELISA using anti-FLAG-HRP (Sigma) or anti-HA-HRP antibodies (Roche Diagnostics) ...
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bioRxiv - Biochemistry 2021Quote: ... or alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for Elisa (Sigma P7998) were added as a substrate ...
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bioRxiv - Physiology 2024Quote: ... Plasma total ghrelin was measured by mouse/rat ELISA (EZRGRT-91K, Millipore). All samples were run in duplicate ...
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bioRxiv - Molecular Biology 2021Quote: ... Heart sections (5 μm) were stained using Masson’s trichrome kit (Sigma-Aldrich# HT15-1KT). The 0.8X and 20X objectives of a Nikon Eclipse 80i microscope were used to capture the whole heart and scar images and ...
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bioRxiv - Physiology 2020Quote: Paraffin sections with 5 μm thickness were stained using Masson’s Trichrome stain kit (Sigma) as described previously.24 In brief ...
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bioRxiv - Plant Biology 2023Quote: ATP was measured using the Adenosine 5’-triphosphate (ATP) Bioluminescent Assay Kit (Sigma-Aldrich) according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... The 5’-pGEM-T construct was then isolated with GenEluteTM Plasmid miniprep kit (Sigma) and digested with 0.5 µl (10 U ...
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bioRxiv - Cancer Biology 2019Quote: ... genomic DNA from each plate was extracted using the GenEluteTM Mammalian Genome DNA miniprep Kit (Sigma). DNA fragments containing transposon/genome junctions were amplified via ligation mediated PCR and sequenced using the Illumina Hi-Seq 4000 platform as previously described (22).
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bioRxiv - Genomics 2019Quote: ... genomic DNA from each plate was collected via GenElute™ Mammalian Genomic DNA Miniprep Kit (Sigma). DNA fragments containing transposon/genome junctions were amplified via ligation mediated PCR and sequenced using the Illumina Hi-Seq 4000 platform.
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bioRxiv - Immunology 2020Quote: ... On day 5, plates were fixed with 20% formalin (Fujifilm Wako Pure Chemical, Japan) and stained with crystal violet solution (Sigma-Aldrich, USA) for the evaluation of cytopathic effect (CPE) ...
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bioRxiv - Cell Biology 2022Quote: ... around 150 young adults were transferred to fresh NGM plates containing 49.5 μM 5-fluoro-2’-deoxyuridine (FUDR, Sigma-Aldrich, MO, USA) to prevent progeny production ...
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bioRxiv - Genetics 2021Quote: ... the worms were transferred onto NGM plates treated with 50 μM of 5-fluoro-2’-deoxyuridine (FUdR, Sigma, St. Louis, MO, USA). Worms were maintained on these plates at 20°C until they were used for experiments at day 1 ...
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed four times 5 min each and 100 μl of blocking solution containing goat anti-mouse IgG (Sigma 1:3,000) was added to each well and incubated at 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Lentiviral vectors were co-transduced with MD2G and PAX2 in 293T cells (5 million cells/10cm plate) with 25ul of XtremeGene9 (Millipore Sigma, #6365787001) and supernatant was harvested at 48hr and 72hr post transfection ...
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bioRxiv - Cell Biology 2021Quote: ... age-synchronized animals at the L4 stage were collected in M9 buffer and plated on NGM plates containing 5-fluoro-2’-deoxyuridine (FUDR, Sigma, 50 μM) to prevent egg-laying (84).
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bioRxiv - Immunology 2020Quote: ... 5 × 106 isolated neutrophils were cultivated in 60 cm2 plate with the addition of 100 nM PMA (Sigma-Aldrich S.r.l.,Milan, Italy) for 4 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... for 36-48 h were plated on the SNS imaging plate along with 2 or 5 unit/mL of PI-PLC (P8804, Sigma Aldrich, USA) in complete growth medium for 1 h and then stained with phalloidin-Alexa 405 prior to imaging ...
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bioRxiv - Plant Biology 2022Quote: ... Individual plants of similar size were transferred onto BCDATG agar (4-5 per plate) supplemented with NaCl or methyl viologen (Sigma-Aldrich, 856177) and incubated at 22±1°C with a 16h:8h light:dark cycle ...