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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Immunoprecipitation was carried out by mixing 500-800 µg of whole cell lysate and 1-2 µg of anti-Flag M2 antibody (Sigma Millipore, #F3165), 1-2 µg of anti-c-Myc antibody (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with secondary antibody in PBS (1:250) anti-Mouse IgG (whole molecule) – fluorescein isothiocyanate (FITC) antibody produced in goat (Sigma, F9006) in dark for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... were incorporated into the multi-cloning site in the pET21a(+) vector (Novagen, Merck KGaA, Germany). The plasmids carrying the microbial rhodopsin genes were transformed into the E ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Whole mount epidermal preparations were stained with Nile Blue (Sigma-Aldrich) and Oil Red O (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... We whole mounted an isolated retina on a nitrocellulose filter (Millipore) with a central 2 x 2 mm hole ...
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bioRxiv - Bioengineering 2019Quote: Mononuclear cells from whole blood were purified by histopaque (Sigma-Aldrich) according to the manufacturer instruction ...
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bioRxiv - Physiology 2021Quote: ... Whole locust ears were fixed in 4% paraformaldehyde (P6148, Sigma Aldrich) dissolved in Phosphate Buffer Saline (PBS ...
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bioRxiv - Cell Biology 2021Quote: Whole-cell lysates were prepared using RIPA buffer (Sigma, Cat # R0278) containing protease inhibitor cocktail (Sigma ...
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bioRxiv - Genomics 2022Quote: ... the whole tissues were rapidly dissected in cold PBS (Sigma-Aldrich), transferred to a microcentrifuge tube containing 100 μl of Trizol (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: Whole-mount immunostaining with anti-α-tubulin antibody (Sigma-Aldrich; #T5168) to label the mitotic spindle was performed as follows ...
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bioRxiv - Developmental Biology 2022Quote: ... Whole cell lysates were prepared using RIPA cell lysis buffer (Sigma) containing 1:100 phosphatase inhibitor cocktail 2 ...
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bioRxiv - Immunology 2020Quote: Whole-cell lystates were harvested in RIPA lysis buffer (Sigma-Aldrich) containing 1 x protease inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2019Quote: Mononuclear cells from whole blood were purified using Histopaque (Sigma-Aldrich) according to the manufacturer instruction ...
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bioRxiv - Developmental Biology 2022Quote: ... whole-cell lysates were prepared in RIPA lysis buffer (Sigma, USA) and aliquots of 15 μg protein were separated by SDS-PAGE and blotted onto a polyvinylidene fluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... Whole-cell lysates were then incubated with Anti-FLAG beads (Sigma) for 2 hours at 4°C and beads were washed four times with lysis buffer.
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bioRxiv - Evolutionary Biology 2022Quote: ... Whole mount epidermal preparations were stained with Nile Blue (Sigma-Aldrich) and Oil Red O (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody (Sigma-Aldrich, A3562) diluted 1/5000 was used as the secondary antibody ...
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bioRxiv - Plant Biology 2022Quote: ... and goat anti-rabbit IgG (whole molecule)–peroxidase antibody (A6154, Sigma) were used at a dilution of 1:50,000 and 1:6,000 ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were extracted as whole cell lysate using trichloroacetic acid (Sigma) and glass beads as described before87.
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bioRxiv - Plant Biology 2023Quote: ... whole seedlings were incubated in 0.01% (w/v) PI solution (Sigma) for 10 min ...
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bioRxiv - Physiology 2024Quote: ... whole kidneys were homogenized in CelLytic MT lysis buffer (Sigma #C3228) at 1:20 weight/volume using a glass homogenizer ...
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bioRxiv - Neuroscience 2023Quote: Whole fly brains were dissected in ice cold RNAlater (Sigma-Aldrich) with 0.1% PBST as per previously published protocol [85] ...
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bioRxiv - Biochemistry 2020Quote: The S-variant and RBD coupled bead sets were incubated with SARS-CoV-2 patient sera at 3 dilutions (1/100; 1/1000 and 1/10000) for 1 h in 96-well filter plates (MultiScreenHTS; Millipore) at room temperature in the dark on a horizontal shaker ...
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bioRxiv - Molecular Biology 2022Quote: ... Plates were blocked with 100 µl/well Reagent Diluent (1% Probumin (Millipore, 82-045-1) in PBS pH 7.2 ...
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bioRxiv - Neuroscience 2021Quote: ... was used to deliver HBSS before and after application of E2 (100 pM diluted from DMSO-stocks in HBSS, 17β-estradiol, Sigma-Aldrich, cat # E-8875) or ATP (30 uM diluted in HBSS ...
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bioRxiv - Microbiology 2022Quote: ... hormones were maintained in the culture medium throughout the course of each assay. 17-ß-estradiol (i. e., E2) and progesterone (i. e., P4) (both from Sigma; St. Louis, MO) were selected for use in infection studies because these hormones represent the major forms of estrogens and of progestogens (respectively ...
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bioRxiv - Physiology 2019Quote: ... 6 dpf larvae in 35 mm Petri dishes were first incubated in 5 mL of steady state E2 medium + 100 µL of Tricaine (Sigma-Aldrich #E10521, Schnelldorf, Germany); they were considered to be anesthetized when they failed to respond to tactile stimulation ...
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bioRxiv - Immunology 2022Quote: ... Cultures were stimulated with 20 ng/ml PMA for 3 hours and assessed with a mouse IFN-γ ELISA kit (Sigma-Millipore). For airway epithelial cell culture ...
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bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected and serum insulin levels were determined in triplicate using a rat/mouse insulin Enzyme-Linked Sorbent Assay (ELISA) kit from Millipore (EZRMI-13K) according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... Phosphorylated ERK was measured by preparing epithelial cells as described above (Colon epithelial cell isolation) and using a Phospho-Erk1 (pThr202 / pTyr204 + Erk2 (pTyr185/187) and pan-Erk1 / 2 ELISA Kit from Sigma (Cat. RAB0349) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Plasma concentrations of acylated ghrelin and leptin were determined by commercial ELISA kits according to the manufacturers’ instructions (Spi Bio #A05117.96 and Millipore #EZML-82K, respectively).
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bioRxiv - Immunology 2021Quote: ... plates were developed with 1 mg/mL OPD (Sigma; P3804-100TAB) in citrate buffer (PBS with 0.05 M NaH2PO4 and 0.02 M Citric acid ...
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bioRxiv - Microbiology 2020Quote: ... CM-glc plates containing 1% (w/v) charcoal (Sigma C-9157) were used for mating assays (Hartmann et al. ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... The plates were coated with fibronectin (1:100, Sigma-Aldrich, F1141) for 2 h at 37 °C before use ...
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bioRxiv - Cancer Biology 2022Quote: ... cultivated on LB-selection plates with ampicillin (1:1000) (Sigma Aldrich). PCR using U6 primer (annealing to vector backbone ...
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bioRxiv - Cell Biology 2020Quote: ... animals were cultured on NMG plates containing 1 mM IAA (Sigma) for 48 hrs prior to imaging.
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bioRxiv - Microbiology 2020Quote: ... Plates were stained with 250 μl of 1% Crystal Violet (Sigma) for 10 −15 minutes ...
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bioRxiv - Immunology 2021Quote: ... Plates were then blocked with PBS 1% BSA (Sigma-Aldrich, A3059) and subsequently incubated with mAb serial dilutions for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Plates were then blocked with PBS 1% BSA (Sigma-Aldrich, A3059) and subsequently incubated with mAb serial dilutions for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Plates were then blocked with PBS 1% BSA (Sigma-Aldrich, A3059) and subsequently incubated with mAb serial dilutions for 1 h at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Picked spheres were plated (20 spheres per well of a 6-well plate) onto Nunclon-Δ plates coated with 0.1 mg mL−1 poly-L-ornithine (Sigma) followed by 10 µg mL−1 laminin (PO/Lam coating ...
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bioRxiv - Bioengineering 2021Quote: ELISpot plates (Millipore IP Filter plate) were coated with 20 µg/mL RBD in sterile PBS overnight at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ELISpot plates (Millipore IP Filter plate) were coated with 20μg/mL Spike in sterile PBS overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... ELISpot plates (Millipore IP Filter plate) were coated with 20 µg/mL heat-inactivated uricase in sterile PBS overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we also determined whole-body triacylglyceride (TAG) content (in µg per fly) using a serum triglyceride determination kit (Sigma Aldrich; Tennessen et al. 2014). For each cage and treatment ...
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bioRxiv - Plant Biology 2022Quote: ... the nuclear fraction was prepared from whole plants using the ‘Semi-pure Preparation of Nuclei Procedures’ protocol of the CelLytic PN Isolation/Extraction Kit (Sigma-Aldrich, St. Louis, MO). Genomic DNA was isolated from the nucleus or whole plant using a DNeasy Plant mini kit (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... Normal pancreata from the tdTomato progeny and from EGLN1/2/3fl/fl mice and whole tumors from KPC mice were minced and digested with 1 mg/mL Collagenase V (Sigma, C9263-500MG) for 30 minutes at 37°C and 130 rpm/min followed by digestion with TrypLE (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: Ixodes IRE1α-FLAG and TRAF2-HA expression was validated by immunoblotting whole cell lysates with α-FLAG-HRP (Sigma, A8592, 1:500) and α-HA (Pierce ...
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bioRxiv - Molecular Biology 2021Quote: ... a clone of the cell line SSN-1 derived from whole fry tissue of snakehead fish (Sigma-Aldrich cat no. 01110916-1VL). The virus was propagated as described in Dong et al ...