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Citations for Millipore Sigma :
1 - 50 of 9906 citations for PLGF Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: HEK 293-T cells (Sigma-Aldrich 12022001) were cultured at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: Human embryonic kidney (HEK) 293 cells were purchased from ATCC and HEK-293 PDE4A4 stable cell line established by Millipore. HEK-293 and HEK-293 PDE4A4 cells were cultured in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Cell Biology 2021Quote: ... HEK 293 cells were obtained from Sigma-Aldrich. The cells were grown in non-coated flasks with DMEM GlutaMAX ...
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bioRxiv - Bioengineering 2021Quote: ... expressed in HEK 293 cells (Sigma-Aldrich, # cat. H8916). The cytokine was added at different concentrations (20 ng/mL ...
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bioRxiv - Cell Biology 2019Quote: ... HEK 293 FT cells were maintained in DMEM (Sigma Aldrich) supplemented with Penicillin Streptomycin and 10% FBS at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2019Quote: ... HEK 293 cells were seeded onto poly-lysine (Sigma P48323) coated 32 mm diameter cover slips (Thermo Scientific/Menzel ...
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bioRxiv - Immunology 2020Quote: ... HEK 293 cells were grown in Dulbecco’s Modified Eagle Medium (Sigma-Aldrich) supplemented with 100 U/ml penicillin ...
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bioRxiv - Biochemistry 2024Quote: HEK-293 cells were seeded on poly-L-lysine (Sigma Aldrich, P4707) coated cover slips in a 24-well plate and transiently transfected the following day with 50-250 ng DNA ...
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bioRxiv - Biochemistry 2021Quote: ... The cell line was cultured at 37 °C under 4.5 % CO2 in EX-CELL® 293 Serum-Free Medium for HEK 293 Cells (Sigma), supplemented with 4 mM L-glutamine ...
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bioRxiv - Genomics 2019Quote: HepG2 and HEK-293 cells were cultured in EMEM medium (Sigma, Cat # M0643) supplemented with 10% FBS (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: Cultured HEK 293 cells were lysed with RIPA buffer (Sigma, St. Louis, MO, R0278) with protease inhibitor cocktail (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... HEK cells were grown in suspension in Ex-Cell® 293 Serum-Free Medium (Sigma) with 2% FBS and were infected at a density of 2×106 cells/mL using a 1/10 (v/v ...
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bioRxiv - Neuroscience 2021Quote: HEK 293 cells stably expressing the D2R (60) were grown on polylysine (Poly-D-lysine hydrobromide, Sigma) and transfected with Tet-On plasmids encoding either the D2R-IL3 or D2R-IL3-scr peptides and the Tag RFP (4 μg per well) ...
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bioRxiv - Neuroscience 2021Quote: ... HEK 293 cell edges were stained with Phalloidine-Atto565 (specific against F-actin) dye (Sigma–Aldrich, Switzerland). Nuclei were counterstained with DAPI (4′,6-diamidine-2′-phenylindole dihydrochloride ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were collected using ice-cold PBS (HEK-293 and A549 cells) or Accutase (Sigma, MDCK cells) to retain surface glycans ...
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bioRxiv - Cancer Biology 2020Quote: ... and DMEM (A-375 and HEK-293) supplemented with 2mM glutamine (Sigma-Aldrich, St; Louis, MO, USA) and 10% FBS (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... and HEK 293 were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich, St Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2020Quote: β2AR-HEK 293 cells were plated onto glass coverslips pre-treated with 0.01% poly L-Lysine (Sigma-Aldrich). Cells were serum starved while cultured in normoxia or hypoxia incubator for 6 hours or stimulated with ISO in normoxia as positive control along with endocytosis inhibitors ...
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bioRxiv - Neuroscience 2019Quote: HEK 293 cells (CRL-1573, A.T.C.C., Manassas, VA, USA; grown in Minimum Essential Medium Eagle (MEM; Sigma M0446) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2019Quote: ... All experiments were carried out on HEK-293 cells with low passage and maintained in Dulbecco’s modified Eagle’s complete media (Sigma) supplemented with 10% fetal bovine serum (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... hERG channel protein was stably expressed in HEK-293 cells purchased from Millipore (Cat. No. CYL3006; Billerica, MA). Assay methods have been previously described (32–34) ...
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bioRxiv - Molecular Biology 2022Quote: HeLa and human embryonic kidney (HEK) 293 cells were cultured in Dulbecco’s Modified Eagle’s medium (high glucose, Sigma) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Baby Hamster Kidney (BHK) and Human Embryonic Kidney (HEK) 293 were grown in Dulbecco’s modified eagle’s medium (DMEM; Sigma) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... and human embryonic kidney (HEK) 293T or 293 cells were cultured in Dulbecco’s modified essential medium (DMEM) (Sigma) with 10% foetal calf serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... HEK 293 cells were seeded at 8,000-10,000 cells/cm2 on type 1 collagen (10 μg/cm2, Sigma-Aldrich) coated 35 mm polystyrene dishes (Falcon ...
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bioRxiv - Microbiology 2023Quote: ... anti-His (mouse, 1:3k, Sigma, A7058), anti-c-Myc (mouse ...
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bioRxiv - Cell Biology 2020Quote: ... Melford Laboratories) and zeocin (DLD-1, 60 μg/ml; HEK-293, 100 μg/ml; HeLa, 50 μg/ml; Sigma Aldrich). Established ovarian carcinoma cell lines OVCAR-3 (ATCC ...
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bioRxiv - Systems Biology 2020Quote: Human embyronic kidney cells (HEK-293 cells) were grown as adherent cultures in DMEM with high glucose (Sigma-Aldrich D5796), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Physiology 2022Quote: ... T25 flasks with HEK-293 cells were transiently co-transfected using X-tremeGENE™ 9 reagent (Sigma-Aldrich, Darmstadt, Germany) with 2 µg of mouse Nav1.5 cDNA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... A constant amount of plasmid cDNA (15 μg) was transfected into human embryonic kidney cells 293 T (HEK-293T) using polyethylenimine (PEI; Sigma) in a 1:2 weight ratio in 10 cm plates ...
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bioRxiv - Microbiology 2020Quote: ... Mouse anti-His antibody (1:12000) (Sigma-Aldrich) and biotinylated PNA (1:6000 ...
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bioRxiv - Molecular Biology 2022Quote: Nuclear extracts from HEK 293 cells expressing either FLAG-tagged CXXC or PHD constructs were incubated with anti-FLAG M2 beads (Sigma Aldrich) in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... HEK T-REx-293 cells were maintained in DMEM supplemented with 10% fetal calf serum and 1% penicillin/streptomycin (Sigma-Aldrich). Non-transfected HEK T-Rex-293 cells were used as control cells.
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bioRxiv - Pharmacology and Toxicology 2020Quote: HEK 293 cells and WT or mutant A1R expressing HEK 293 cells were harvested using a non-enzymatic cell dissociation solution (Sigma-Aldrich) and washed with PBS prior to counting ...
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bioRxiv - Molecular Biology 2023Quote: ... with either the wild type Cacna2d2-3’UTR or the Cacna2d2 3’UTR bearing mutations in the miR-423-5p binding sites (Cacna2d2 mut 3’UTR) in HEK 293 cells (Millipore Sigma). Briefly ...
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bioRxiv - Cell Biology 2022Quote: A431 (CRL-1555, ATCC), MDCK (CCL-34, ATCC) or HEK-293-T (CRL-3216) cells were cultured in DMEM (Sigma-Aldrich), supplemented with 10% Fetal Bovine Serum (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... T-REx-293 and HEK-293T cells were cultured in DMEM media (Dulbecco’s Modified Eagle’s Medium; Sigma-Aldrich, Gillingham, UK #D5796), whilst CHO-CAR and CHO-ACE2-TMPRSS2 cells were cultured in DMEM-F12 media (Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham ...
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bioRxiv - Systems Biology 2024Quote: The MIA PaCa-2 pancreatic cancer and HEK 293 human embryonic kidney were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma Aldrich, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: Transiently expressed antibodies were purified from 30 ml of HEK 293 Expi cultures using Protein G GraviTrap columns (Sigma, GE28-9852-55) and the Ab Buffer Kit (Sigma ...
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bioRxiv - Immunology 2022Quote: ... viral supernatant from HEK-293 cells was added to B16 or MC-38 cells with 5 mg/ml of polybrene (Sigma-Aldrich H9268) and cells were spun down at 800 XG for 90 minutes at 30 degrees ...
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were detected using α -His from mouse (Sigma H1029), α-Gfp from mouse ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The rHA proteins have a molecular weight of ∼270 kDa and were expressed in HEK-293 cells and purified using IMAC Sepharose® High Performance resin (Sigma Aldrich, St. Louis, MO). Viruses were from the Influenza Reagents Resource (IRR ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-His antibody (Sigma; 1:5,000 in PBS + 2% BSA) was added and incubated for 1h ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Biophysics 2023Quote: ... then incubated with mouse anti-His tag primary antibody (Millipore Sigma SAB1305538) at 1:1,000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... with the following modifications: Mouse anti-GST tag or mouse anti-His tag 1:200× (Sigma-Aldrich) and HRP-conjugated goat anti-mouse IgG 1:2,000× (Seracare Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... respectively (Millipore Cat# HI-14K, HI-13K). Samples treated with 3 mM glucose KRBH were measured undiluted ...