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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for PGFM ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Vero cells grown on 96-well cell culture plates were treated with cell culture medium (DMEM with 5% fetal bovine serum) supplemented with acyclovir (ACV; Sigma), in concentrations of 128 µg/ml to 0.03125 µg/ml (1:4 serial dilutions) ...
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bioRxiv - Molecular Biology 2019Quote: ... U2OS cells were seeded in a 6-well plate and infected with lentivirus supernatant in the presence of 5 μg/mL polybrene (Sigma). Medium was replaced by fresh media with 10 μg/mL Blasticidin S at 24 hours post-infection ...
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bioRxiv - Microbiology 2021Quote: ... subtilis strains are listed in Table 1 and were grown in BHIS broth or on LB agar plates supplemented with CAM (2.5 μg mL−1) and/or tetracycline (TET, 5 and 10 μg mL−1, Millipore Sigma).
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bioRxiv - Microbiology 2021Quote: ... the plates were washed 5 times with 200 μL of 1x PBST 100 μL of pNPP (Sigma, Cat. No. N2770) was added to each well ...
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bioRxiv - Molecular Biology 2021Quote: ... Cardiac myocytes were then plated at a density of 0.5 × 106 cells per well on 34.8 mm plastic plates that had been pre-treated with 5 μg/mL fibronectin (cat# F1141, Sigma-Aldrich) in serum free DMEM/F12 medium (cat# 11330032 ...
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bioRxiv - Microbiology 2021Quote: ... Thereafter 10 μl of the culture was streaked on SA (1% lactose or glucose) agar plate supplemented with 10 μg/ml 5-fluoroorotate (Sigma). Plates were incubated at 30°C until 5-fluoroorotate resistant colonies emerged ...
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bioRxiv - Neuroscience 2022Quote: Primary cultures of mouse astrocytes were grown in vitro in the plates coated with 5 μg/mL fibronectin (Sigma, F2006) for 4-5 days before they were infected with lentivirus ...
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bioRxiv - Neuroscience 2022Quote: NGM plates (60 mm) were seeded with 250 μL OP50 mixed with 5 μL 100 mM all-trans retinal (Sigma) as previously described (Piggott et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% FBS were seeded in each well of a 96 well MultiScreen® HV filter plate (EMD Millipore) placed on top of a 96 well collection plate ...
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bioRxiv - Microbiology 2023Quote: ... a dilution series of 107 to 103 cells/ml were plated in 5 μl spots on M9 minimal media plates with 10 mM glucose or 10 mM glucose-6-phosphate (Sigma) and antibiotics ...
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bioRxiv - Genetics 2023Quote: ... RPL28 mutants were generated by growing BG14 to saturation overnight in YPD medium and plating onto YPD plates containing 5 µg/mL of cycloheximide (Sigma). Colonies were purified on YPD plates and screened by PCR-seq for mutations in RPL28 using primers listed in Sup ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting cells were then plated on Spec/Kan agar plates supplemented with 5% sucrose and 0.05% acetamide (Sigma-Aldrich), to induce msmeg_0317 deletion via the second crossover ...
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bioRxiv - Synthetic Biology 2023Quote: ... 0.5 μL) into a clear round-bottom 96-well plate (Costar) pre-filled with 200 μL of 1X PBS (Sigma) supplemented with tetracycline (10 μg/mL) ...
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bioRxiv - Synthetic Biology 2024Quote: 100,000 cells were plated in a 96 well round bottom plate with either 5 µM MG132 (Sigma-Aldrich #M7449-200UL), 1 µM MLN4924(Active Biochem #A-1139) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and then seeded in 6-well plates (5×105 cells/well) that had been pre-coated the day before with type I collagen (Sigma) dissolved in 0.02 N acetic acid ...
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bioRxiv - Bioengineering 2023Quote: ... Plates were washed again and anti-FLAG M2 antibody (Sigma-Aldrich, 1:2000 dilution in 5% milk/0.1% PBS-T) was added and incubated for 1hr at room temperature ...
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bioRxiv - Physiology 2022Quote: The mNGF level in tears upon a AAV2/9 CAG-mNGF injection in the LG was dosed using the mNGF ELISA kit (Sigma-Aldrich, reference RAB1119, France) following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: Cryopreserved blood plasma was thawed at room temperature and used for IL17a detection by a commercial mouse IL-17a ELISA Kit (Sigma Aldrich catalog #RAB0263-1KT). The test was performed according to manufacturer guidelines ...
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bioRxiv - Neuroscience 2020Quote: ... ELISA reaction procedure was followed as described by manufacturer (OPD; Sigma). The optical density of the supernatant was read on a spectrophotometer at 492 nm ...
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bioRxiv - Physiology 2020Quote: ... Mouse insulin and C-peptide were detected by multiplex ELISA (Millipore) kit in the plasma samples collected during the oral glucose challenge ...
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bioRxiv - Neuroscience 2022Quote: ... Multiplex ELISA was performed on a Luminex FlexMAP 3D (Millipore Sigma) using a premixed 32-plex cytokine magnetic bead panel (Millipore Sigma ...
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bioRxiv - Physiology 2021Quote: ... 100 μl of 3,3’,5,5’-tetramethylbenzidine (TMB) Elisa substrate (T0440; Sigma) was added and the reaction stopped after 10 min using 2 M H2SO4 ...
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bioRxiv - Genetics 2020Quote: ... Adiponectin ELISA (mouse Adiponectin, EZMADP-60K, EMD Millipore Corporation, Darmsbalt, Germany) and Cholesterol Assay (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Leptin concentrations were determined by ELISA (Millipore, St. Charles, MI, USA). The blood glucose levels were measured using a Glucocard glucometer (Arkray ...
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bioRxiv - Immunology 2023Quote: ... All ELISA assays were developed using TMB liquid substrate system (Sigma), stopped with 2N sulfuric acid ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Cytokines and CCL5 were measured by Multiplex ELISA (MCYTOMAG-70K, Millipore) or Enzyme Linked Immunosorbent Assay (ELISA ...
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bioRxiv - Immunology 2022Quote: ... Components C through F are the same for all 5 kits (Sigma-Aldrich) and are as follows ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 μM 5-Aza-2′-deoxycytidine (5-Aza; Sigma), 250-500 nM CpG-scramble ...
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bioRxiv - Molecular Biology 2020Quote: ... HCT116 shNT and shDHX30 cells were cultured in 96-well tissue culture plate and treated with 100mM 5-Fluorouracil (Sigma Aldrich) as positive control ...
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bioRxiv - Developmental Biology 2019Quote: ... on gelatin-coated plates and grown for 5 days in the presence/absence of L-Pro (250–500 μM) (Sigma-Aldrich), with a medium change at day 3 ...
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bioRxiv - Genomics 2021Quote: ... and sorting G1 cells based on Hoechst staining on an Influx FACS machine into 384 well plates containing 5 μl sterile filtered mineral oil (Sigma Aldrich) per well ...
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcriptase qPCR was performed in a Framestar-480/384 well plate containing 5 µl of 2x SYBR Green JumpStart Taq ReadyMix (Sigma-Aldrich), 2 µl cDNA ...
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bioRxiv - Microbiology 2020Quote: Bacterial plate counts were performed on lysogeny broth (LB-Miller) agar plates (10 g tryptone, 5 g yeast extract, 10 g NaCl, and 15 g agar (all from Sigma-Aldrich) in 1 L of Millipore water) ...
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bioRxiv - Cell Biology 2021Quote: ... single cell clones were generated by serial dilution in 96-well plates and maintained in growth media with 200 μg/mL Zeocin and 5 μM methotrexate (MTX, EMD Millipore). CHO-SEAP CMV::XRCC6 ATM+ PRKDC+ was generated analogously to CHO-K1 ATM+ PRKDC+ ...
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bioRxiv - Evolutionary Biology 2022Quote: ... bacterial densities were determined by plating out 100 µl of a dilution series ranging from 10−5 to 10−7 on Vibrio selective Thiosulfate Citrate Bile Sucrose Agar (TCBS) plates (Sigma Aldrich). Plates were incubated over night at 25° C and the total amount of colonies was counted the following day.
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bioRxiv - Immunology 2022Quote: Lysates from cells treated in 6-well plate were heated for 5 minutes at 100 ºC with the same volume of Leammli Buffer 2X (Sigma-Aldrich). Samples were separated in a vertical electrophoresis on 12% polyacrylamide gels (Biorad ...
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bioRxiv - Molecular Biology 2022Quote: ... until 10-7 and 10 µl of the dilutions between 10-5 and 10-7 were plated in BHI agar plates supplemented with Congo Red (Sigma®) at a final concentration of 25 µg.ml-1 ...
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bioRxiv - Neuroscience 2022Quote: ... UAS-CsChrimson/UAS-Aion) were grown in darkness on grape agar plates with yeast paste containing 5 mM all-trans-retinal (Sigma-Aldrich). Staged third instar larvae (96 h ± 3 h AEL ...
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bioRxiv - Microbiology 2021Quote: ... 5 µl of the reaction mixture (in duplicate) was quenched into a MultiScreenHTS Durapore 96-well filter plate (MSHVN4B50; Millipore Sigma) containing 200 µl of 10% charcoal in 0.5% HCl and 50 µl of wash buffer (1 M HCl with 200 mM NaPPi) ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus-containing supernatants were collected 48 h later and added with Polybrene to AML cells pre-seeded at ∼5 × 105/well in 24-well plates (Sigma-Aldrich). After 8 h ...
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bioRxiv - Microbiology 2022Quote: ... 5 μL was used to inoculate 200 μL of LB broth containing 5 μg/mL of tobramycin in a well of a 96-well plate (Corning, Sigma-Aldrich). An additional 5 μL was added to a corresponding well of another 96-well plate containing 200 μL of LB broth ...
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bioRxiv - Cell Biology 2022Quote: ... trypsinized cells were resuspended in standard growth medium without antibiotics and plated in 6-well culture plates (5 × 104 cells/well) after mixing with 10 µg/ml polybrene (EMD Millipore) and lentivirus supernatant ...
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bioRxiv - Genomics 2019Quote: ... at which point the fibrin gels were sufficiently polymerized around the posts to be lubricated in media and transferred from the casting troughs into a 24-well plate with fresh 3D-EHT media (RPMI-B-27 with penicillin/streptomycin, and 5 mg/mL aminocaproic acid, Sigma-Aldrich). 3D-EHTs were supplied with 2.5 mL/well of fresh 3D-EHT media three times per week.
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bioRxiv - Cell Biology 2021Quote: ... at which point the fibrin gels were sufficiently polymerized around the posts to be lubricated in media and transferred from the casting troughs into a 24-well plate with fresh 3D-EHT media (RPMI-B27 with penicillin/streptomycin, and 5 mg/ml aminocaproic acid; Sigma-Aldrich). 3D-EHTs were supplied with 2.5 ml/well of fresh 3D-EHT media three times per week.
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bioRxiv - Neuroscience 2021Quote: ... plated at a density of 2-5 × 104 cells /well on coverslips in a 24-well plate pre-coated with poly-L-lysine (Sigma, P9155) and cultured in a Neurobasal medium supplemented with 2% B27 supplement ...
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bioRxiv - Cancer Biology 2021Quote: ... GSCs used were between passages 5 and 30 and cultured either as spheres or as attached on fibronectin-coated plates (10ug/ml, Millipore Sigma) in a medium of 1X Neurobasal Medium ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed 5 times with PBS/0.5% Tween-20 and 50µl of HRP-anti-mouse-IgA (Sigma-Aldrich, 1:1000) was added to each well ...
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bioRxiv - Microbiology 2022Quote: ... A yeast colony was picked from a YSM-Trp plate and resuspended in 100 µl of 5% w/v Chelex-100 (Sigma-Aldrich). Half the sample volume of acid washed glass beads were added and the sample vortexed at maximum speed for 4 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 3 to 5 isolated morphologically normal colonies selected from the agar plates were suspended in cation adjusted Mueller Hinton broth (CAMHB, Sigma-Aldrich). The bacterial suspensions were diluted with normal saline to achieve a turbidity equivalent to a 0.5 McFarland standard (latex ...
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bioRxiv - Cell Biology 2023Quote: ... cells were serially diluted 10-fold and 2 μl of each dilution was spotted on plates containing 5 mM hydroxyurea (Sigma-Aldrich) or on YEPD and exposed to UV with a dose of 200 J/m2 ...