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Citations for Millipore Sigma :
201 - 250 of 5016 citations for P selectin Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 1% P/S (#P0781, Sigma), 25 mM Hepes (#9157.1 ...
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bioRxiv - Molecular Biology 2021Quote: ... SC-His+3AT is minimal medium of SC-His supplemented with 3-aminotriazole (3-AT, 0.5mM, Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... Removal of His-tag was verified by western blot with monoclonal α-His –HRP (1:10000) antibody (Sigma).
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bioRxiv - Plant Biology 2020Quote: ... coli using a Nickel-His column (Sigma). A 5 mL culture was incubated at 37°C for 16 hours ...
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bioRxiv - Cell Biology 2020Quote: ... anti-His tag (HIS.H8, 1:1,000, Millipore). The HRP-conjugated anti-mouse (111–035-144 ...
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bioRxiv - Plant Biology 2020Quote: ... -His with or without 3AT (Sigma-Aldrich). Successful interactions were further screened on –Leu ...
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bioRxiv - Microbiology 2020Quote: ... The anti-his antibody (monoclonal #8722 Sigma) was used at 1/2000 concentration for immunoblot.
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bioRxiv - Biochemistry 2022Quote: ... and anti-His (1:3000) antibody (Sigma). HRP conjugated mouse IgG was used at 1:5000 dilution (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-His HRP conjugated (Sigma-Aldrich) antibodies ...
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bioRxiv - Microbiology 2021Quote: ... HIS-Select® Nickel Affinity resin (Sigma) and Streptactin column material (IBA GmbH ...
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bioRxiv - Immunology 2020Quote: ... cOmplete His-Tag Purification Resin (Sigma Millipore) was applied for affinity purification ...
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bioRxiv - Immunology 2020Quote: ... cOmplete His-Tag Purification Resin (Sigma Millipore) was applied for affinity purification ...
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bioRxiv - Plant Biology 2020Quote: ... and purified on His•Bind resin (Novagen) in the presence of 8 M urea according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... anti-His monoclonal antibody (Sigma, 1:1000) and anti-mouse (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with His-HDAC6 (EMD Millipore) and 1 mM ATP in kinase buffer for 1 h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... then detected with anti-His antibody (Sigma). S protein expression was detected with anti-SARS-CoV-2 S protein S2 subunit mouse mAb unconjugated (GeneTex) ...
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bioRxiv - Immunology 2020Quote: ... with added 10% HI FBS (Sigma-Aldrich) and 1% Pen/Strep (Lonza) ...
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bioRxiv - Cell Biology 2021Quote: ... or anti-HIS (1:10,000, Sigma H1029) was used ...
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bioRxiv - Neuroscience 2021Quote: ... amino acids (His, Ser, Ala, Trp; Sigma) were diluted to a final concentration of 10-4 M and bile acid (TDCA ...
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bioRxiv - Microbiology 2023Quote: ... anti-His (mouse, 1:3k, Sigma, A7058), anti-c-Myc (mouse ...
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bioRxiv - Biochemistry 2022Quote: ... anti-His (Millipore: 05-949, clone HIS.H8) or Goat anti-Mouse IgG (Millipore ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and His-Bind Purification kit (Novagen, USA) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... L-histidine (L-his, Sigma-Aldrich #H8125), L-ornithine (L-orn ...
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bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
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bioRxiv - Neuroscience 2019Quote: ... the transfected HEK 293T cells were treated with MG132 (Sigma-Aldrich) at a concentration of 20 μM for 16 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK-293T were grown in Hepes-modified DMEM (Sigma-Aldrich, D6171), supplemented with 10% FCS (Eurobio Scientific ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged recombinant proteins were detected using an HRP-conjugated anti-His monoclonal antibody (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... His- and GST-tagged proteins were detected with anti-His (Monoclonal Anti-polyHistidine antibody, Sigma H1029, 1:5000 dilution) or anti-GST (Mouse anti-GST monoclonal antibody ...
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bioRxiv - Microbiology 2019Quote: ... were cultured in suspension in serum-free Ex-Cell 293 medium (Sigma) and Expi293 medium (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... HEK293E cells were cultured in EX-CELL 293 serum-free medium (Sigma) and incubated with agitation at 37°C and 4.5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 293 cells were cultured in MEM medium (Sigma-Aldrich, St. Louis USA) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... and isotopically-labeled d9-PC P-36:1 (P-18:0/18:1, >99%, Sigma, 852475C) and d4-palmitic acid (d4-C16:0 ...
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bioRxiv - Biophysics 2023Quote: ... Alkaline phosphatase activity was determined by the hydrolysis of p-nitrophenyl phosphate (p-NPP, Sigma-Aldrich) in a reaction medium containing 10 mM p-NPP and 1 mM MgCl2 in AMPOL buffer (2-amino-2-methyl-1-propanol) ...
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was eluted in a gradient of His-AC washing buffer and His-AC elution buffer (both supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...
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Controlled Fluorescent Labelling of Metal Oxide Nanoparticles for Artefact-free Live Cell MicroscopybioRxiv - Biophysics 2021Quote: ... 1% P/S (Penicillin-Streptomycin, Sigma), 1% NEAA (non-essential amino acids ...
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bioRxiv - Cell Biology 2020Quote: ... p-eIF2α (Ser 51) (SIGMA, SAB4504388), GADD34/PPP1R15A (Abclonal ...
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bioRxiv - Physiology 2022Quote: ... poloxamer-407 (P-407; Sigma, P2443) was dissolved in ice-cold saline (250 mg/kg ...
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bioRxiv - Immunology 2022Quote: ... and the Phytohemagglutinin (PHA-P, Sigma) and CELL STIMULATION COCKTAIL (ThermoFisher ...
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bioRxiv - Developmental Biology 2019Quote: ... and 2.5% Pancreatin (SIGMA, P-3292) for 4h at 4°C on individual wells of a 12 well plastic dish ...
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bioRxiv - Developmental Biology 2020Quote: ... containing protease inhibitors (P-8340 Sigma) and phosphatase inhibitors (Calbiochem 524629 ...
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bioRxiv - Microbiology 2019Quote: ... 1% penicillin-streptomycin (P/S) (Sigma). Vero cells ...
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bioRxiv - Cell Biology 2019Quote: ... P-PDH (EMD Millipore, ABS204, RRID:AB_11205754)
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bioRxiv - Genetics 2019Quote: ... transferred to Immobilon-P membrane (Millipore) and probed with antibodies essentially as described previously 20 ...
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bioRxiv - Neuroscience 2019Quote: ... transferred to Immobilon-P membrane (Millipore) and probed with antibodies essentially as described previously7 ...
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bioRxiv - Microbiology 2021Quote: ... transferred on Immobilon-P membrane (Millipore) and analyzed by immunoblotting with the polyclonal rabbit antibodies raised against OutB ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1% P/S (Sigma Aldrich), cells were stained with BCAM BV421 (clone B64 ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1mg/mL Streptomycin (Sigma P-0781) and 200mM L-Glutamine (Sigma G-7513) ...