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Citations for Millipore Sigma :
351 - 400 of 420 citations for Opioid receptor antagonist since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... sera were heat-inactivated at 56 °C for 30 minutes and treated with receptor destroying enzyme (Sigma) in a 1:4 mixture (5x dilution of sera) ...
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bioRxiv - Neuroscience 2022Quote: ... AMPA-receptor-mediated excitatory postsynaptic currents (EPSCs) were recorded in presence of picrotoxin (100 μM, Sigma Aldrich) to block GABAARs ...
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bioRxiv - Bioengineering 2023Quote: ... The stimulation of ACh to muscarinic receptors was blocked by addition of 10 nM Atropine (Sigma Aldrich). In the presence of Atropine ...
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bioRxiv - Neuroscience 2024Quote: ... 2) GluR2 to visualize postsynaptic receptors (mouse anti-GluR2 at 1:2000, Millipore, catalog # MAB397, RRID: AB_2113875) and 3 ...
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bioRxiv - Neuroscience 2024Quote: ... Aladdin) or β receptor agonist isoprenaline (2 mg/mL, 4 mg/mL, n = 7, I5627, Sigma-Aldrich) or β receptor antagonist propranolol (2·5 mg/mL ...
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bioRxiv - Biophysics 2020Quote: ... Active A2AR was eluted from the column by flowing purification buffer containing 20 mM theophylline (low-affinity A2AR antagonist, KD = 1.6 μM; Sigma, St. Louis, MO, USA; #T1633). Western blot analysis was performed to determine 4-mL fractions with active A2AR collected with a BioFrac fraction collector (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... des-Arg9-Leu8]-BK (B1R antagonist) and 3-(5′-Hydroxymethyl-2′-furyl)-1-benzyl indazole were purchased from Sigma-Aldrich (St. Louis, MO, USA). 8-Bromoguanosine 3′ ...
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bioRxiv - Biochemistry 2020Quote: ... we intraperitoneally (i.p.) administrated the AMPA/kainate receptor agonist kainate (8 mg/kg; Sigma, St. Louis, MO, USA) dissolved in 0.1 M phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... a selective inhibitor of TGFβ Type 1 receptor kinases (TGFR1i) was purchased from Sigma-Aldrich (St. Louis, MO) and used at a concentration of 10 µM in all experiments.
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bioRxiv - Biophysics 2021Quote: ... The receptor solution was subsequently centrifuged at 130’000 g through a 0.22-μm Ultrafree-MC spin filter (Millipore) for 5 minutes at 4°C in order to remove possible aggregates ...
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bioRxiv - Neuroscience 2022Quote: ... AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptors were blocked using 6,7-dinitroquinoxaline-2,3-dione (DNQX, Sigma) dissolved in dimethyl sulfoxide and diluted by 1000 in aCSF for 10μM bath application.
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bioRxiv - Biophysics 2023Quote: ... and the Transient Receptor Potential Vanilloid (TRPV4) agonist GSK1016790A (300 nM [Sigma-Aldrich] dissolved in modified Tyrode solution) were applied using a local perfusion system ...
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bioRxiv - Biochemistry 2024Quote: The collected concentrated receptors from the previous step (1.5 mg/mL) were mixed with asolectin lipids (Sigma-Aldrich) solubilized at 5 mg/mL in 5% DDM (Anatrace) ...
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bioRxiv - Biophysics 2020Quote: ... was mixed gently in an Erlenmeyer flask for 20 h at room temperature with 48 mL of DMSO containing 24 mg of xanthine amine congener (XAC, high-affinity A2AR antagonist, KD = 32 nM; Sigma, St. Louis, MO, USA; #X103). The absorbance at 310 nm of the XAC-DMSO solution before and after the coupling reaction was measured in 10 mM HCl and compared to a standard curve ...
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bioRxiv - Neuroscience 2021Quote: ... Vasoconstriction was induced by the application of the thromboxane A2 receptor agonist U46619 (9,11-dideoxy-11a,9a-epoxymethanoprostaglandin F2α, 50 nM, Sigma) for 2 min ...
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bioRxiv - Neuroscience 2022Quote: ... and NMDA receptor mediated PSCs were blocked with DL-2-amino-5-phosphonopentanoic acid (D-AP5; 50 μM; Sigma). The AMPA-R PSC frequency and amplitude was determined from a 1 min interval after the recording had stabilized (~10 min after wash-in of picrotoxin/D-AP5) ...
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bioRxiv - Neuroscience 2021Quote: Rats were subcutaneously injected with NMDA receptor co-agonist D-SER (s.c. D-SER 10mg/Kg, Sigma-Aldrich, Ireland) in vehicle ...
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bioRxiv - Biochemistry 2021Quote: ... Blot was stripped and re-probed for total receptor using HRP conjugated anti-FLAG M2-antibody (Sigma, 1: 5000). The signal was normalized with respect to total receptor and quantified using ImageLab software (BioRad).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Effect of glucocorticoid receptor modulators on osteogenic differentiation of MSCs was quantified using Alizarin Red S (Sigma-Aldrich, A5533) staining ...
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bioRxiv - Neuroscience 2021Quote: ... Vasoconstriction was induced by the application of the thromboxane A2 receptor agonist U46619 (9,11-dideoxy-11a,9a-epoxymethanoprostaglandin F2α, 50 nM, Sigma) for 2 min ...
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bioRxiv - Immunology 2021Quote: ... Diphtheria toxin receptor (DTR) and control mice were given 200 ng intraperitoneal injections of diphtheria toxin (Sigma Cat #D0564) on three consecutive days prior to transplant ...
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bioRxiv - Physiology 2023Quote: ... mice were given the highly-selective ß3-adrenergic receptor agonist CL-316,243 (CL; Sigma; 1 mg/kg body mass) dissolved in saline ...
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bioRxiv - Biochemistry 2023Quote: ... Solubilised receptors were harvested by centrifugation at 45,000 rpm for 1 hour and supplemented with 10 mM imidazole (Sigma) before loading onto a Nickel-NTA (Ni2+ ...
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bioRxiv - Neuroscience 2020Quote: ... Spontaneous IPSCs were isolated by blocking AMPA receptor-mediated events with CNQX (6-cyano-7-nitroquinoxaline-2,3-dione; 20uM; Sigma-Aldrich). Miniature events were isolated by blocking sodium channels with the addition of tetrodotoxin (1uM ...
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bioRxiv - Neuroscience 2021Quote: ... were dissolved in magnesium-free HEPES-aCSF in the presence of non-NMDA glutamate receptor blockers (CNQX, Tocris, 0190, 40 μM; Nimodipine, Tocris, 0600, 1 μM; LY-367385, Sigma, L4420 ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... The role of mannose receptor was evaluated by incubating cells with HIV-1 in the presence of 100µg/mL BMA (Sigma-Aldrich) or 100µg/mL bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2020Quote: ... Responsiveness to NGF was assayed by probing for the expression of tropomyosin receptor kinase A (Trk-A; 1:2000; #06-574, Millipore-Sigma) [48] ...
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bioRxiv - Neuroscience 2019Quote: ... Sheared crosslinked chromatin was immunoprecipitated separately with an anti-AR antibody (3 μg, 17-10489 ChIPAb + androgen receptor Assay Kit, Millipore) or an equal amount of normal IgG (3 μg ...
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bioRxiv - Neuroscience 2019Quote: ... We then removed the supernatant and incubated the lysates on ice for 1 hour with rabbit antibody to D2 receptors (1 μg per 100 μL, Millipore). Anti-rabbit Ig IP beads were added and samples were incubated overnight at 4°C with gentle agitation ...
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bioRxiv - Biochemistry 2020Quote: ... The Flag-tagged receptor was detected using mouse anti-Flag M2 primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #F3165) and IRDye® 680RD-conjugated donkey anti-mouse IgG secondary antibody (1:20,000 dilution ...
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bioRxiv - Neuroscience 2021Quote: The following odors/concentrations were used for comparing molecular properties to receptor responses: 1% 2-methyl-2-pentenal (Sigma 294667), 1% trans-cinnamaldehyde (Sigma C80687) ...
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bioRxiv - Neuroscience 2021Quote: ... received bilateral infusions of either vehicle (aCSF; pH 7.4; males n = 5; females n = 4) or the GABAA receptor agonist muscimol (Sigma Aldrich, M1523 ...
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bioRxiv - Neuroscience 2022Quote: ... Currents of 5-HT3A receptors and engineered constructs expressed at the cell surface were evoked by applying 10 μM serotonin creatinine sulfate complex (Sigma), or in short 5-HT ...
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bioRxiv - Neuroscience 2022Quote: ... The expression of the receptor was quantified at both molecular weight forms using anti-DRD1 primary (Sigma Aldrich, cat#D2944) at 1:1000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... and was mixed with 1 µg total DNA (100 ng receptor plasmid + 200 ng mGs plasmid + 700 ng salmon sperm DNA (Sigma) solubilized in 100 µL OptiMEM ...
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bioRxiv - Neuroscience 2023Quote: ... and the AMPA receptor inhibitor 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 µM, Sigma Aldrich, St Louis, MO, USA). This solution was applied to the autaptic culture neurons for 2 min ...
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bioRxiv - Neuroscience 2023Quote: ... were dissolved in HEPES-aCSF in the presence of non-NMDA glutamate receptor blockers (CNQX, Tocris, 0190, 40 µM; Nimodipine, Tocris, 0600, 1 µM; LY-367385, Sigma, L4420 ...
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bioRxiv - Immunology 2024Quote: ... 8-Cyclopentyl-1,3-dipropylxanthine (DPCPX)(28, 33, 34) or A1 receptor agonist 2-Chloro-N6-cyclopentyladenosine (35) were purchased from Sigma Aldrich, dissolved in DMSO and filter sterilized by passing through a 0.22μm filter prior to use ...
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bioRxiv - Physiology 2023Quote: NIH-3T3-L1 cells were incubated in DMEM containing 2% fatty-acid free BSA in the presence of exogenous mitochondria and/or CL316,243 (β3-adrenergic receptor agonist; Sigma Aldrich) for 72 h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and incubated with mouse primary anti-Collagen Type II (DSHB II-II6B3) or mouse anti-dopamine 4 receptor (MABN125, Millipore) primary for 2 h at room temperature in a humidified chamber ...
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bioRxiv - Cell Biology 2022Quote: ... as well as the role of myosin II/RhoA in creating the refractory nature of the retracting losing front to receptor inputs (Fig. 5 and Supplementary Fig. 2) we pre-treated cells with either 50 μM nocodazole (Sigma Aldrich) or 10 μM Υ-27632 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Responsiveness to NGF was assayed by probing for the expression of tropomyosin receptor kinase A (Trk-A; 1:2000; #06-574, Millipore-Sigma) [48] ...
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bioRxiv - Neuroscience 2020Quote: ... Dimethyl sulfoxide (DMSO) and clozapine-N-oxide (CNO; agonist at hM3Dq receptors) were purchased from Sigma-Aldrich (St. Louis, MO, USA). A stock solution of CNO (0.1 mg/ml in saline solution containing 0.5% DMSO was prepared and stored in aliquots ...
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bioRxiv - Cell Biology 2021Quote: To explore the role of myosin II/RhoA and ERM proteins/cortical actin in creating a cell back refractory to receptor inputs we pre-treated cells with either 20 µM Υ-26732 (Sigma), or 50 µM Nocodazole (Sigma ...
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bioRxiv - Neuroscience 2022Quote: Two immortalized cell lines were used to validate the ACE2 receptor, TMPRSS2 and NRP1 assays: Human oligodendroglioma cells (HOG, Cat# SCC163) were obtained from Millipore Sigma-Aldrich (Billerica ...
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bioRxiv - Biochemistry 2021Quote: ... The blot was stripped and then reprobed for FLAG-tagged receptor using anti-FLAG antibody (1:2000, Sigma, Cat. no. A8592). Data were quantified using ImageLab software (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... For the blockade of cholinergic receptors in CA1 200nl of atropine (2 mM) and mecamylamine (2 mM) (1:1) (Sigma Aldrich) were microinjected at 1.2mm DV (IM-9B microinjector ...
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bioRxiv - Neuroscience 2022Quote: Larvae were immobilized by immersion in a paralytic that specifically blocks nicotinic acetylcholine receptors present at the neuromuscular junction (20μL of 1 mg/mL α-bungarotoxin [Sigma Aldrich] in HEPES-buffered E3 [5 mM NaCl ...
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bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS containing 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with the following antibodies ...
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bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS + 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with CD15-APCCy7 (1:100 ...