Labshake search
Citations for Millipore Sigma :
101 - 150 of 3052 citations for OGG1 Human GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... protein was purified by incubating with glutathione agarose (GST; Sigma Aldrich) or amylose resin (MBP ...
-
bioRxiv - Cell Biology 2021Quote: ... GST-VAPB was expressed in BL21 Rosetta (DE3) cells (EMD Millipore) induced with 1 mM IPTG for 4 h ...
-
bioRxiv - Biochemistry 2023Quote: ... Protease was removed by incubation with GST-Bind beads (EMD Millipore). Sid4 was concentrated using Amicon Ultra centrifugal filters (Millipore) ...
-
bioRxiv - Molecular Biology 2019Quote: ... GST (27457701; GE-Healthcare, 1:2000) and Tubulin (T6199; Sigma, 1:5000). Donkey anti-Rabbit 800CW (926-32213 ...
-
bioRxiv - Microbiology 2021Quote: ... beads containing bound GST-PCT+N complex were incubated with thrombin (Novagen) for 16 h at 20°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... running on SDS-PAGE and western blotting with anti-GST (Sigma G1160).
-
bioRxiv - Developmental Biology 2019Quote: ... and GST-Flag-psmd4b were treated with Thrombin (1:1000; T4648, Sigma) to cleave their GST tags ...
-
bioRxiv - Biochemistry 2020Quote: ... Bound PEX14-NTD was immunodetected with monoclonal anti-GST antibodies (Sigma-Aldrich) in TBS plus 3% BSA ...
-
bioRxiv - Plant Biology 2020Quote: OsDMI3-GST fusion protein was produced in Escherichia coli Rosetta (DE3; Novagen), and tested for interaction as described previously (Ni et al. ...
-
bioRxiv - Microbiology 2020Quote: ... GST bound protein was then cleaved using biotinylated thrombin (Merck Millipore; 69672) according to the manufacturers instructions overnight at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-GST HRP Conjugated antibody (Sigma Aldrich; diluted 1:5,000 in PBS) was added to each well (100 μL/well ...
-
bioRxiv - Neuroscience 2022Quote: ... the expression of GST fusion proteins was induced with isopropylthiogalactoside (IPTG, Sigma) at 16 °C overnight ...
-
bioRxiv - Biophysics 2023Quote: ... GST was cleaved using the Thrombin CleanCleave kit (Sigma-Aldrich cat # RECOMT) for 14 hours at 4 °C with gentle rocking ...
-
bioRxiv - Cell Biology 2023Quote: ... or Glutathione High Capacity Magnetic Agarode Beads (for GST-tagged proteins, SIGMA). After 2 extensive washing with binding buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... GST-Cdk1/CyclinA2 at 20 μg/mL (Sigma-Aldrich, stock ref. C0244) and GST-Cdk2/CyclinA2 at 20 μg/mL (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2023Quote: ... and GST-Cdk2/CyclinA2 at 20 μg/mL (Sigma-Aldrich, ref. C0495). Then ...
-
bioRxiv - Molecular Biology 2020Quote: ... overexpression of GST-β1a was carried out using Escherichia coli BL21 (DE3) (Novagen) transformed with plasmid containing the cDNA inserted into pGEX-4T-2 vector ...
-
bioRxiv - Neuroscience 2021Quote: ... the GST tag was cleaved using the Thrombin CleanCleave Kit (Sigma-Aldrich, SLBZ7194) according to the manufacture’s protocol after elution step ...
-
bioRxiv - Biochemistry 2020Quote: ... Proteins were then resolved by SDS-PAGE and immunoblotted using GST (Sigma Aldrich), 6His (Sigma Aldrich ...
-
bioRxiv - Plant Biology 2021Quote: ... as well as with anti GST antibody (Cat no. G7781-2ML, Sigma, USA). GST protein was used as a control for all the pull-down experiments ...
-
bioRxiv - Microbiology 2022Quote: ... GST-tagged proteins were purified under native conditions on gluthatione-agarose resin (Sigma). The recombinant proteins were then washed 3 times with Buffer A (100mM KCl ...
-
bioRxiv - Plant Biology 2021Quote: ... GST-PPKs were tested against 5 µg myelin basic protein (MBP; Sigma Aldrich) and 5 µg Casein in 0.1 M Tris pH 8.8 (Sigma) ...
-
bioRxiv - Plant Biology 2022Quote: ... 50 µg GST–CAR9 protein emulsified with Incomplete Freund’s adjuvant (F5506, Sigma–Aldrich) was injected into the ICR mouse and then once again in the next week ...
-
bioRxiv - Molecular Biology 2019Quote: ... the slide was incubated with anti-GST antibody (Sigma G7781; 1:13,000 dilution) in 5% dried milk in TBS-T for 1hr at 23°C ...
-
bioRxiv - Cell Biology 2020Quote: ... actin 224-236-1 (DSHB Iowa University) and GST clone 6G9C6 (Sigma-Aldrich); rabbit polyclonal anti-GFP/EGFP PABG1 (Chromotek) ...
-
bioRxiv - Microbiology 2020Quote: ... Fractions containing GST-paFur were cleaved using a Thrombin CleanCleave kit (Sigma-Aldrich). Briefly ...
-
bioRxiv - Developmental Biology 2022Quote: ... A GST-Drn fusion protein was produced in Origami B (DE3) cells (Novagen) and purified with a glutathione-Sepharose 4B column ...
-
bioRxiv - Cell Biology 2022Quote: Enzymatic cleavage of the GST tag was performed using thrombin (Cat# T1063, Sigma). Supernatants containing cleaved target proteins were incubated with Benzamidine Sepharose 4 flat flow (Cat# 17-5123-10 ...
-
bioRxiv - Cell Biology 2022Quote: ... The expressed GST-fusion protein was absorbed to glutathione-Sepharose 4B (Sigma-Aldrich) followed by extensive washing with phosphate buffered saline (PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... and the GST tag was removed by cleaving with PreScission Protease (Sigma-Aldrich). The purified proteins were exchanged to the biotinylation buffer containing 25 mM HEPES ...
-
bioRxiv - Biochemistry 2022Quote: pCold-GST GTSF-expression vectors were transformed into Rosetta-Gami 2 competent cells (Novagen). Cells were grown in the presence of 1 μM ZnSO4 at 37°C until OD600 ∼0.6–0.8 ...
-
bioRxiv - Cell Biology 2022Quote: The recombinant GST-tagged Nanobody against GFP69 expressed in Escherichia coli Rosettagami (Novagen, 71351) was purified with Glutathione Sepharose 4B beads according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... and the GST tag was cleaved by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4 °C ...
-
bioRxiv - Microbiology 2020Quote: ... The membrane was incubated with HRP conjugated anti-GST secondary antibody (Sigma –Aldrich, USA) at 1:5000 dilution for one hour ...
-
bioRxiv - Cell Biology 2021Quote: ... Western Blot analysis was carried out using Anti-GST(1:3000) antibody (Sigma #G1160) and Anti-His(1:3000 ...
-
bioRxiv - Genetics 2020Quote: The GST-Clr4 fusion protein was allowed to bind to Glutathione-agarose beads (Sigma) in MTPBS buffer (100mM Na2HPO4 ...
-
bioRxiv - Genetics 2020Quote: L4 gst-4p::gfp expressing worms were anesthetized using 2 mM Levamisole (Sigma L9756) and mounted on 2% agarose pad ...
-
bioRxiv - Biophysics 2023Quote: ... GST-tagged Elf1 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Glutathione Sepharose 4 Fast Flow resin (GE Healthcare) ...
-
bioRxiv - Plant Biology 2023Quote: ... at 1:3,000 dilution or anti- GST primary antibody (cat. no. SAB4200692; Sigma-Aldrich) at 1:2,000 dilution ...
-
bioRxiv - Cell Biology 2024Quote: ... and GST (#GE27-4577-01, goat polyclonal, Sigma Aldrich; used at 1:10,000 dilution). HRP-conjugated anti-goat and anti-rabbit secondary antibodies (Millipore ...
-
bioRxiv - Cell Biology 2020Quote: ... The in vitro interaction experiments using recombinant Ana1 fragments and commercial GST-PBD (Sigma, SRP0360) were carried out as described previously (Alvarez-Rodrigo et al. ...
-
bioRxiv - Developmental Biology 2021Quote: ... GST-tagged Pros-S-HD in soluble fraction was purified using Glutathione-Agarose beads (Sigma). The bound proteins were eluted in elution buffer (50 mM Tris ...
-
bioRxiv - Neuroscience 2023Quote: ... GST-α synuclein fusion protein tagged at the N-terminus was obtained from Sigma Aldrich. 200 µg of GST-α synuclein was incubated with the equivalent amount of purified His-spectrin at 4°C overnight in the presence of glutathione sepharose beads ...
-
bioRxiv - Genetics 2023Quote: ... Protein expression was verified and quantified by Western blot using recombinant GST protein (Sigma, G5663) as expression standards ...
-
bioRxiv - Biochemistry 2021Quote: Human IgM purified from human serum (Sigma) was digested with trypsin ...
-
bioRxiv - Microbiology 2021Quote: ... Putative ligand proteins (elastin from human skin, fibrinogen from human plasma, laminin from human placenta, fibronectin from human plasma) (all from Sigma) were resuspended in carbonate-bicarbonate buffer (Sigma ...
-
bioRxiv - Immunology 2020Quote: ... or human (1:20000; anti-human IgG; Sigma) for 1 hour at room temperature ...
-
bioRxiv - Biochemistry 2022Quote: ... and the GST tag was cleaved on column by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4°C ...
-
bioRxiv - Biophysics 2021Quote: ... GST-GFP-RGG protein aliquots were concentrated with a centrifugal concentrator (Amicon Ultra Centrifugal Filters, Millipore). Proteins were then mixed with 20 mM Tris ...
-
bioRxiv - Molecular Biology 2020Quote: ... The proteins were eluted by protease cleavage of the GST-tag (thrombin or PreScission (Sigma-Aldrich) were used depending on the enzyme availability ...