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Citations for Millipore Sigma :
201 - 250 of 9915 citations for Notch1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 2% GlutaMAX (200 mM ...
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bioRxiv - Cell Biology 2024Quote: ... +10% HI FBS(Sigma Aldrich, cat#F4135-500ML) + penicillin/streptomycin (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-His-primary antibody (Sigma, 1:2,000) was incubated with the protein-treated lipid strips to detect Jps1 bound via its C-terminal His-tag ...
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bioRxiv - Immunology 2019Quote: ... HEK-293T cells were plated on poly-L-Lysine-coated (Sigma; P4707) 10 cm plates (Corning ...
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bioRxiv - Cell Biology 2022Quote: BEAS-2B and HEK-293T cells (ATCC) were grown in DMEM (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: HEK 293T cells were cultured in DMEM with 10% FBS (Millipore Sigma), GlutaMAX (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... HCC5044 and HEK-293T cells were cultured in DMEM (Sigma-Aldrich, D5796) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2023Quote: ... TPC-1 and HEK 293T/17 were cultured in DMEM (Sigma D6429) supplemented with 10% FBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Bioengineering 2024Quote: ... typically in an equal mixture of Expi293 and EX-CELL 293 (14571C Sigma). Expi293 media can be used for the entire process ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Immunology 2022Quote: HEK 293T cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, D6429) supplemented with 10% heat inactivated FBS (Atlanta Biologicals ...
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bioRxiv - Biochemistry 2021Quote: ... HEK-293T cells were grown in in Dulbecco’s Modified Eagle Medium (DMEM, Sigma) with the addition of 10% fetal bovine serum ...
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bioRxiv - Neuroscience 2019Quote: HEK 293T cells were transiently transfected using X-treme gene 9 (Sigma Aldrich). After 48 hours of the transfection ...
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bioRxiv - Biophysics 2019Quote: ... All HEK cell culture media were supplemented with 10% foetal calf serum (Sigma) and 1% Pen/Strep (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... The antibodies were transiently expressed in HEK 293A cells following polyethylenimine (Sigma-Aldrich) transfection (Sherbenou et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK 293T cells were transfected with Cdt1 expression plasmids using PEI Max (Sigma) and cultured for 16 hours ...
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bioRxiv - Cell Biology 2022Quote: ... HEK cell culture medium contained Dulbecco’s modified eagle medium (D6046, Sigma-Aldrich, Germany). Both media were supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... at a ratio of 3:2:1 in HEK 293T cells (Sigma Aldrich) using Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... 293T HEK and MEF in Dulbecco’s modified Eagle’s medium (Sigma Aldrich, D6046-500ML). The culture media was supplemented with 10% foetal bovine serum (Sigma Aldrich ...
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bioRxiv - Genomics 2023Quote: ... the cells were exposed to HEK media with 1 µg/mL doxycycline (Sigma) to induce expression of the BFP/iCasp9 from the landing pad ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
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bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
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bioRxiv - Biochemistry 2021Quote: ... recombinant His-Akt1 was from EMD Millipore (#14-279); recombinant GST-mTORf (containing a 30 fragment of mTOR encoding amino acids 2144-2175 ...
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK 293T cells were transfected with pLVX-FAP-TRF1 and Mission Packaging Mix (Sigma) to produce lentivirus ...
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bioRxiv - Biophysics 2019Quote: ... AAVs were generated in HEK 293T cells and purified by iodixanol (Optiprep, Sigma; D1556) step gradients as described (Challis et al. ...
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bioRxiv - Genomics 2021Quote: ... Both hPBMCs and HEK 293T cells were cryopreserved in FBS + 10% DMSO (Sigma-Aldrich). Prior to FACS sorting ...
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bioRxiv - Cancer Biology 2022Quote: HEK-293T cells (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2019Quote: ... 1 x 105 HEK 293T cells/well were seeded onto poly-lysine (Sigma; 7405) coated 24-well plates ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK-3.x cells were detached from the flask using trypsin/EDTA (Sigma-Aldrich) and resuspended in 10 mL Leibovitz’s L-15 media supplemented with 1% FBS ...
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bioRxiv - Cancer Biology 2022Quote: Human HEK-293T cells transfected with either LacZ shRNA (control) or IKKα shRNAs (Sigma) along with their corresponding packaging plasmids were used to prepare lentiviruses from cell culture medium 48 h after transfection ...
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bioRxiv - Microbiology 2023Quote: The total RNA from Ace2-HEK-293T cells was isolated using TRI Reagent (Sigma) as per manufacturer’s protocol ...
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bioRxiv - Bioengineering 2019Quote: The His-TEV tag was cleaved using TEV protease (Sigma). The reaction was made overnight at 4°C into dialysis buffer for TEV (25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2019Quote: ... HIS-TAN1-GFP was conjugated with Atto488-maleimide (Sigma 28562). 4μM HIS-TAN1-GFP in 80mM PIPES ...
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bioRxiv - Immunology 2021Quote: ... This was followed by 0.5ug/mL anti-his HRP (Sigma), and binding was detected after incubation with substrate at an absorbance of 450nm ...
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bioRxiv - Biochemistry 2020Quote: ... The antibodies used for western blotting were anti-His (Sigma), anti-Strep HRP conjugated (iba ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
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bioRxiv - Plant Biology 2020Quote: ... and 1:5000 dilution of primary anti-His (SIGMA, USA), 1:10000 for anti-GST (SIGMA ...
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bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng recombinant His-AKT1 (inactive, Upstate/Millipore #14-279), at a final volume of 30 μl ...
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bioRxiv - Biochemistry 2020Quote: Recombinant Ded1-His was expressed from the pET22b plasmid (Novagen) and purified as previously described (46) ...
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bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
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bioRxiv - Biochemistry 2021Quote: α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-Cul2 (EPR3104 ...
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bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.