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Citations for Millipore Sigma :
1 - 50 of 8548 citations for Norovirus GII.4 VLP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the bound NoV capsid proteins were detected with mouse anti norovirus GII (1:1000; NativeAntigen) and by adding horseradish peroxidase conjugated with goat anti-mouse IgG (1:1500; Sigma Aldrich). Horseradish peroxidase activity was detected with a TMB (3,3’,5,5’-tetramethylbenzidine ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 % glycerol for GII RTs) with an Amicon Ultra-15 (30k) concentrator (Millipore) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: The naturally packaged ssRNA in Qβ-VLPs was digested as following: Qβ(ssRNA)-VLPs were washed 3x with 20mM HEPES (Sigma-Aldrich) using Amicon ultra 0.5ml tubes 100kDa MWCO ...
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bioRxiv - Microbiology 2023Quote: ... VLP and cells were stained with SYBRGreen I (Sigma) for 10 min in the dark at 80°C and let cool down for 5 min at room temperature [46] ...
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bioRxiv - Synthetic Biology 2019Quote: ... plates were coated with indicated concentrations of VLP (4°C, over-night) and subsequently blocked with 1% casein in PBS (Sigma-Aldrich) for 2h at 37°C before primary antibodies were added (polyclonal mouse anti-Pfs230 or monoclonal 1B3 antibody ...
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bioRxiv - Biophysics 2019Quote: ... Purified PCV2 VLPs were concentrated and buffer exchanged to phosphate buffered saline (PBS) using Amicon Utra-4 centrifugal filter devices (Merck Millipore, MA). PCV2 VLP samples were stored in 50-100 µl aliquots at −80°C.
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bioRxiv - Cell Biology 2019Quote: ... Supernatants containing VLPs were filtered (Millex HV, 0.45 μm; Millipore) and frozen at −80°C until use ...
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bioRxiv - Microbiology 2023Quote: ... The VLPs were concentrated with Polyethylene glycol 8000 (Sigma-Aldrich, Cat#P2139), overnight precipitation and purification by chloroform treatment ...
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bioRxiv - Molecular Biology 2021Quote: ... All lentivectors and VLPs were DNAse treated (2 h at 37°C, DNaseI, Sigma) before they were concentrated by ultracentrifugation in a Sorvall Discovery (Hitachi ...
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bioRxiv - Microbiology 2023Quote: ... We collected VLP bands and buffer exchanged using a 100-kDa Amicon filter (Sigma). VLPs were buffer exchanged into DPBS (Thermo Fisher Scientific Cat # ...
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bioRxiv - Microbiology 2021Quote: ... VLP-containing supernatant was filtered through a 0.2 um sterile syringe filter (Millex-GP, Millipore Sigma, USA) to remove the remaining bacteria ...
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bioRxiv - Microbiology 2022Quote: ... The VLP pellet was suspended in 1 mL SM buffer with lysozyme (10.0 mg/reaction; Sigma Aldrich) and incubated for 60 min at 37 °C with gentle shaking to degrade unfiltered bacterial cells ...
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bioRxiv - Microbiology 2024Quote: ... and VLPs were concentrated 10 times by the addition of PBS/8 % (w/v) PEG 6000 (Sigma), incubation at 4° C for 24 h ...
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bioRxiv - Evolutionary Biology 2022Quote: ... VLP pellets were lysed in 60 μl of NP-40 lysis buffer containing 1% NP-40 (Sigma-Aldrich) and 10 μg/ml each of aprotinin ...
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bioRxiv - Microbiology 2021Quote: ... The VLP-containing supernatant was filtered through a 0.2 μm sterile syringe filter (Millex-GP, Millipore Sigma, USA) concentrated by centrifugation (35,000g ...
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bioRxiv - Immunology 2021Quote: ... Qβ(1668)-VLPs were then derivatized for 30min at RT using dibenzocyclooctyne NHS ester (DBCO cross-linker) (Sigma-Aldrich) in a 5-fold molar excess ...
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bioRxiv - Microbiology 2020Quote: ... VLP samples were separated by 10% or 14% SDS-PAGE later blotted to PVDF membrane (Immobilon®-FL, Merck Millipore) and blocked overnight with ODYSSEY® blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... all CIS43 VLPs and wild-type (WT) Qβ VLPs were depleted of endotoxin by three rounds of phase separation using Triton X-114 (Sigma-Aldrich), as described in reference 48 ...
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bioRxiv - Immunology 2022Quote: ... or MDM (106) were treated for 1h at 37°C with 1 ml of VLPs-VPX+ containing 5 μg/ml polybrene (Sigma, #H9268) for SAMHD1 degradation16,37 before the addition of 20 µg CAp24 of each control or/and encoding-gene lentiviral vectors with 5 μg/ml polybrene for 2h transduction ...
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bioRxiv - Molecular Biology 2023Quote: ... ChAC-VLPs was further concentrated to 5 times of its initial volume using 100 kDa cut-off concentrator (Merck, Millipore, USA) followed by analysis on 10% Tris-Glycine SDS-PAGE and successful RBD-E2 fusion protein is validated by western blot using anti-RBD antibody.
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bioRxiv - Microbiology 2023Quote: ... VLPs were lysed by the addition of 100 μl of Phage Lysis Buffer (4.5 M guanidinium isothiocyanate (Sigma-Aldrich Cat#50983), 44 mM sodium citrate pH 7.0 (Sigma-Aldrich Cat#C8532) ...
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bioRxiv - Microbiology 2023Quote: ... The VLPs were subsequently concentrated down to 180 µL using Amicon Ultra-15 100K Centrifugal Filter Units (Milipore Sigma, MO, U.S.A). To remove any free contaminating nucleic acids ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was filtered using 0.2um filter and VLPs were captured in a 40-10% sucrose step gradient and concentrated using AMICON spin filters (UFC901008, EMD Millipore, Burlington, MA). Total protein yield for purified VLP stock varied somewhat but was typically above 5 mg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... 4-hydroxytamoxifen (4-OHT) (Sigma) was prepared by dissolution in ethanol (10% ...
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bioRxiv - Developmental Biology 2019Quote: ... 4-Hydroxytamoxifen (4-OHT; Sigma) was included where indicated at 10 μM.
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bioRxiv - Developmental Biology 2021Quote: 4-hydroxytamoxifen (4-OHT, Sigma) was dissolved in 100% ethanol to prepare a stock concentration of 10 mM ...
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bioRxiv - Cancer Biology 2021Quote: 4-hydroxytamoxifen (4-OHT, Sigma, #7904) was dissolved in 100% ethanol (1 mM stock solution) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4-hydroxytamoxifen (4-HT [Sigma, H7904]) was dissolved in methanol to a concentration of 5 mg/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... or 4-Hydroxytamoxifen (4-OH, Sigma) was dissolved to a final concentration of 1mg/ml or 3mg/ml in 90% corn oil (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 1μM 4-hydroxytamoxifen (4-OHT; Sigma) was added to select wells 5 days prior to imaging to allow for adequate Cre-mediated recombination and expression of MCT4 shRNA ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... 4 × 10−4 M monothioglycerol (Sigma), 50 µg/mL ascorbic acid (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: 4-PBA (4-phenylbutyric acid, Sigma) and 5-PVA (5-phenylvaleric acid ...
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bioRxiv - Developmental Biology 2019Quote: ... 4-Methylumbelliferone (4-MU, Sigma-Aldrich) was dissolved in DMSO to a stock concentration of 100mM ...
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bioRxiv - Cancer Biology 2021Quote: ... 4-hydroxytamoxifen (4-OHT, Sigma, H7904) was dissolved in 100% DMSO at a concentration of 20mg/ml and 25 ul of the 4-OHT solution was injected via intramuscular injection into the left mouse gastrocnemius muscle ...
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bioRxiv - Developmental Biology 2021Quote: 4-hydroxytamoxifen (4-OHT; Sigma H7904) stock was prepared by dissolving the powder in ethanol ...
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bioRxiv - Cancer Biology 2022Quote: 4-hydroxytamoxifen (4-OHT, Sigma H6278) stock was prepared by dissolving the powder in ethanol to reach 5 or 10 mM concentration and thoroughly vortexed and aliquoted into 100 μl aliquots ...
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bioRxiv - Neuroscience 2022Quote: ... 4 aminopyridine (4-AP, 100µM; Sigma). The currents recorded at the same imposed potential were averaged for each cell to perform the quantification ...
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bioRxiv - Neuroscience 2022Quote: ... 4-aminopyridine (4-AP, Sigma, 275875), and gaboxadol (Sigma ...
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bioRxiv - Immunology 2022Quote: ... 4-hydroxytamoxifen (4-OHT from Sigma) was administered i.p ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... 4-hydroxytamoxifen (4-OHT; Sigma Aldrich) was diluted in ethanol following the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2023Quote: 4-hydroxytamoxifen (4-OHT; Sigma, H7904) was dissolved in corn oil/ethanol (90% corn oil ...
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bioRxiv - Neuroscience 2023Quote: ... 4-Hydroxytamoxifen (4-OHT; Sigma #H6278) was dissolved in corn oil according to a modification of published methods [24] ...
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bioRxiv - Developmental Biology 2023Quote: 4-Hydroxytamoxifen (4-OHT, H7904, Sigma) was dissolved in ethanol to final concentration of 10 mM ...
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bioRxiv - Neuroscience 2023Quote: 4-hydroxytamoxifen (4-HT, Sigma-Aldrich) was dissolved in 100% ethanol (10 mg/ml) ...
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bioRxiv - Developmental Biology 2024Quote: 4-hydroxytamoxifen (4-HT, H7904; Sigma) was dissolved in ethanol at a final stock concentration of 5 mM and kept in single-use aliquots in the dark at –80°C ...
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bioRxiv - Developmental Biology 2023Quote: ... 4-hydroxytamoxifen (4-OHT; Sigma-Aldrich) was administrated to 12 weeks old male mice at a dose of 1.0mg/40g body weight via intraperitoneal injection for five consecutive days ...
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bioRxiv - Cell Biology 2024Quote: 4-hydroxytamoxifen (4-OHT, Sigma H6278) was dissolved at 20 mg/mL in ethanol by shaking at 37°C for 15 minutes and was then aliquoted and stored at -20°C for up to several weeks ...
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bioRxiv - Developmental Biology 2024Quote: 4-Hydroxytamoxifen (4-OHT) Treatments and Bromodeoxyuridine Incorporation: 4-OHT (T176, Sigma) treatment was performed as previously described (Mosimann et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... and 4×10-4 M monothioglycerol (Sigma)) with BMP4 (1 ng/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... 4-nitroquinoline 1-oxide (4-NQO) (Sigma). Treatment conditions are indicated in the corresponding figures ...