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Citations for Millipore Sigma :
501 - 550 of 9542 citations for Native Human ELANE Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... containing 5% human serum (Sigma, Darmstadt, Germany) for 24 hours (1 million cells per well in 96-well plates ...
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bioRxiv - Developmental Biology 2024Quote: ... or 20% anti-human serum antibody (Sigma), depending on the species ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μg/ml human insulin (Sigma-Aldrich), 10 ng/ml recombinant human EGF (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... chicken anti-human MAP2 (EMD Millipore, AB5543), mouse anti-human synapsin (SYSY,106011) ...
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bioRxiv - Microbiology 2023Quote: ... HRP-conjugated anti-human IgG (Sigma-Aldrich), HRP-conjugated anti-mouse IgG (EMD Millipore) ...
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bioRxiv - Molecular Biology 2023Quote: ... then blocked with human IgG (#I4506 Sigma) for 10 minutes at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% human serum (Sigma Aldrich). Cell media was changed every 48 hours ...
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bioRxiv - Genomics 2023Quote: ... 10 μg/mL recombinant human insulin (Sigma), 2 IU/mL heparin (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... human S100A9 (clone 6B4, Millipore Sigma, MABF854), mouse S100A9 (clone 372510 ...
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bioRxiv - Bioengineering 2024Quote: ... VLDL from human plasma (Millipore Sigma, 437647), human serum albumin (Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Anti-human IgM-alkaline phosphatase (Sigma Aldrich) (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... 10% human adult AB serum (Sigma-Aldrich) and 5 ng/mL GM-CSF (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Sigma-Aldrich, UK). NK cells were expanded with cytokines IL-2 (500 U/ml) ...
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bioRxiv - Microbiology 2024Quote: ... 5 ng of recombinant human AChE (Sigma) or 30 μg of tissue lysate was incubated with steroidal alkaloid compound or at room temperature for 30 minutes in 50 mM Tris pH 8.0 in a total volume of 90 μL ...
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bioRxiv - Physiology 2024Quote: ... Human male plasma was obtained from Sigma (H4522 ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant human IFN-α2a (H6041; Sigma-Aldrich) was reconstituted in PBS with 0.1% BSA and added to the basolateral pole at a final concentration of 100 U/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... 3% human AB serum (Sigma-Aldrich, H4522), 3 U/ml heparin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... HDL from human plasma (Sigma-Aldrich, SAE0054), LDL from human plasma (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... LDL from human plasma (Sigma-Aldrich, SAE0053), VLDL from human plasma (Millipore Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... type IV (human placenta; Sigma-Aldrich C5533) and type V (human placenta ...
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bioRxiv - Cell Biology 2024Quote: ... 10nM [Leu15]-Gastrin I Human (G9145, Sigma), 10uM Y-27632 (1254 ...
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bioRxiv - Cell Biology 2024Quote: ... 0.025% Human Insulin Solution (Sigma Aldrich, I9278), 0.5X N2 (Gibco ...
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bioRxiv - Immunology 2024Quote: ... and 10% human AB serum (Sigma, H4522).
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bioRxiv - Bioengineering 2024Quote: Human serum was obtained from Sigma-Aldrich. Rat serum was obtained from Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Antibody bound DNA-protein complexes were isolated by protein G plus/protein A agarose beads (EMD Millipore), washed and eluted ...
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bioRxiv - Bioengineering 2021Quote: Protein quantification using Micro BCA protein assay (Pierce™ BCA Protein Assay Kit, Sigma Aldrich, Poole, UK) was carried out following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Membrane proteins were separated from cytosolic proteins by ultracentrifugation or a ProteoExtractR Transmembrane Protein Extraction Kit (Novagen). Protein concentration was measured with a bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was equally adjusted using Bradford protein assay (Sigma).
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bioRxiv - Synthetic Biology 2020Quote: ... Protein was concentrated in 10,000 MWCO Amicon protein concentrators (Sigma), protein concentration was assayed by Qubit ...
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bioRxiv - Genetics 2020Quote: ... Proteins were quantified by Bradford Protein Assay Kit (Sigma-Aldrich). Protein samples were separated by 4-12% Bis-Tris Protein Gels (Thermo Fisher ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was determined using Bradford protein assay reagent (Sigma), measuring absorbance at a wavelength of 595 nm on a UV/Visible spectrophotometer (Ultrospec 2100 pro ...
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bioRxiv - Cell Biology 2019Quote: ... Protein A and Protein G-Sepharose (Sigma-Aldrich, #P3391, #P3296), IgG sepharose 6 Fast Flow (#GE17-0969-01).
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bioRxiv - Biochemistry 2022Quote: ... and the protein stained using ReadyBlue protein stain (Sigma-Aldrich). Fluorescent DNA bands and protein bands were quantified using the Bio-Rad Image Lab software 6.0.1.
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bioRxiv - Bioengineering 2020Quote: ... Negatively selected human monocytes (Astarte Biologics) were suspended in RPMI 1640 supplemented with 2% human AB serum (Sigma-Aldrich), 1% GlutaMax (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 (human embryonic kidney) and U2OS (human bone osteosarcoma) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich) supplemented with 10 % FBS ...
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bioRxiv - Bioengineering 2023Quote: ... Detection of IgG1 employed anti-human IgG (VH+VL) (Jackson) and of IgA2 anti-human IgA (Hc only) (Sigma). Commercial antibodies were used at dilutions recommended by manufacturer ...
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bioRxiv - Biochemistry 2024Quote: Human embryonic kidney cells (HEK293T) and human fibroblast cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma Aldrich), supplemented with fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2022Quote: ... Protein G (Sigma) was coupled at 10 µg/mL in the presence of 10 mM sodium acetate buffer pH 4.5 at 30 µL/min for 300 seconds until ∼3500-4000 RU was immobilized ...
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bioRxiv - Microbiology 2021Quote: ... Protein G (Sigma) was covalently coupled following activation ...
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bioRxiv - Biophysics 2020Quote: ... Protein G (Sigma) at 10 μg/mL was coupled in the presence of 10mM sodium acetate buffer pH 4.5 at 30 μl/min for 300 seconds in various channels ...
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bioRxiv - Systems Biology 2021Quote: ... purified LC and naïve CD4+ T cells or TRMs were co-cultured in human serum supplemented media (RPMI, Gibco, UK, 10% human serum, Sigma, UK ...
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bioRxiv - Cancer Biology 2020Quote: Human neutrophils were obtained from fresh human peripheral blood from healthy volunteers and were isolated using a Percoll (Sigma-Aldrich) gradient.
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bioRxiv - Microbiology 2020Quote: ... Membranes were probed with polyclonal goat anti-human ACE2 (1:500, AF933, R&D Biosystems) or rabbit anti-human beat-Actin (1:2500, 6L12, Sigma) followed by donkey anti-goat IRdye 680CW or goat anti-rabbit IRdye 800CW (Abcam) ...
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bioRxiv - Physiology 2020Quote: ... For insulin clearance during an insulin challenge, mice were given human insulin (1 U/kg, NovoRapid) or human C-peptide (50 µg/kg, Sigma) by intraperitoneal injection and blood samples were collected by tail vein sampling at 0 ...
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bioRxiv - Microbiology 2020Quote: ... membranes were probed with 1 in 10,000 dilution of human anti-SIC followed by 1 in 50,000 dilution of HRP-conjugated goat anti-human IgG (Sigma-Aldrich). To directly compare detection of SIC fragments by purified human and rabbit anti-SIC antibodies ...
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bioRxiv - Cell Biology 2022Quote: The human alveolar carcinoma epithelial cell line A549 (ATCC, CCL-185) and human colorectal adenocarcinoma cell line HT29 (Sigma Aldrich) were utilized for toxicological evaluation ...
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bioRxiv - Microbiology 2020Quote: ... 15 µl of secondary antibody (anti human Fab-HRP: Jackson ImmunoResearch, cat# 109-035-097; anti human IgG-HRP: Sigma, cat# A6029-1ML ...
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bioRxiv - Immunology 2022Quote: ... alpha enolase (in-house expressed human) and collagen II (bovine purified, chondrex) and at 0.5 μg/ml hIgG1 to citrullinated fibrinogen (human purifed, Sigma Aldrich).
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bioRxiv - Microbiology 2023Quote: Primary human macrophages (MΦs) were cultured in macrophage medium (RPMI-1640 GlutaMAX™ (Thermo) supplemented with 4% human AB serum (Sigma), 100 μg/ml penicillin-streptomycin (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: Protein carbonylation was determined by OxyBlot Protein Oxidation Detection Kit (Millipore). Wild-type animals were synchronized to the young adult stage ...