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Citations for Millipore Sigma :
51 - 100 of 9941 citations for NT 3 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Small interfering RNA (siRNA) to human 3’ UTR PKC-θ was purchased from Sigma Aldrich. YTS-2DL1 cells were transfected with siRNA specific for the indicated genes ...
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bioRxiv - Immunology 2019Quote: ... Coverslips were washed with PBS and coated with 3% human serum albumin (HSA; A1653, Sigma) o.n ...
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bioRxiv - Bioengineering 2024Quote: ... A 3 μL drop of 2.5 U/mL human thrombin (Sigma-Aldrich, cat. no. T6884) was added to each well of the platform ...
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bioRxiv - Cell Biology 2023Quote: ... human Spindly siRNA (Sequence antisense: 5’-GAAAGGGUCUCAAACUGAA-3’, dTdT overhangs, Sigma-Aldrich, St. Louis, MO, USA), and control siRNA (D-001810-10-05 ...
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bioRxiv - Microbiology 2023Quote: ... The total oligo length was 120 nt and was ordered from Millipore Sigma’s standard oligo service ...
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bioRxiv - Genetics 2024Quote: ... mouse anti-APC (NT, clone Ali 12.28) (1:1000, Millipore Sigma, Cat. # MAB3785), rabbit anti-APC (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... Calu-3 (human epithelial lung adenocarcinoma) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma #M4655) supplemented with 10% FCS ...
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bioRxiv - Immunology 2021Quote: Lentivirus based knockdown of human ATG16L1 (5’-ACTGTAGCTTTGCCGTGAATG-3’) was performed using MISSION shRNA constructs (Sigma-Aldrich) and psPAX2 packaging system ...
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bioRxiv - Cell Biology 2023Quote: ... human Ndc80/Hec1 siRNA (sequence antisense: 5’-GAAUUGCAGCAGACUAUUA-3’, dTdT overhangs, Sigma-Aldrich, St. Louis, MO, USA), human KIF4A siRNA (sc-60888 ...
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bioRxiv - Microbiology 2021Quote: O395-NT was tracked in solutions of the linear polymer polyvinylpyrrolidone (PVP K90, Sigma 81440 ...
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bioRxiv - Microbiology 2021Quote: Calu-3 (human epithelial lung adenocarcinoma) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma, Cat#M4655) supplemented with 10% (upon and after viral infection ...
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bioRxiv - Microbiology 2021Quote: Calu-3 (human epithelial lung adenocarcinoma) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma, Cat#M4655) supplemented with 10% (upon and after viral infection ...
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bioRxiv - Developmental Biology 2021Quote: ... Human NTOs were permeabilized and blocked using .3% Triton X (PanREAC AppliChem) and 0.5% BSA solution (Sigma-Aldrich) for 30 m ...
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bioRxiv - Cancer Biology 2022Quote: ... Beads were washed once with NT-2 buffer supplemented with 1mg/mL heparin (Sigma-Aldrich) for 5min at 40C with rotation ...
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bioRxiv - Developmental Biology 2023Quote: ... Rtf1 or NT (non-target) shRNA lentivirus harboring puromycin resistance were purchased from Sigma-Aldrich. mESCs were transduced with the indicated virus at a multiplicity of infection of 1 and selected in 1.2 μg/ml puromycin ...
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bioRxiv - Immunology 2021Quote: ... in conjunction with pLKO.1 lentiviral vector targeting GFP or human c-Rel at a ratio of (1.5:3:4) using X-tremeGENE HP DNA transfection reagent (Sigma). 48h and 72h later viral particle containing media was harvested ...
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bioRxiv - Evolutionary Biology 2023Quote: ... GATA 3 (SAB2100898) (rabbit) 1:400 dilution for zebrafish tissue and 1:100 dilution for human tissue (Sigma Aldrich) and and CD44 (SAB1405590 ...
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bioRxiv - Genetics 2023Quote: ... or a shRNA specific for human SOX11 (100% identity to the equine SOX11 sequence) (5’-CTGGTGGATAAGGATTTGGAT-3’ clone NM_003108.3-1235s1c1; Sigma-Aldrich) were created by transfection into HEK293T packaging cells ...
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bioRxiv - Developmental Biology 2024Quote: ... NTs were incubated for 15 min at 37°C in 1.5 ml Accutase (Sigma Israel, A6964) containing 30 ul Papain (Sigma Israel ...
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bioRxiv - Cancer Biology 2023Quote: ... ASAP1 or control NT (day 0) and followed by Puromycin (3µg/ml, Sigma-Aldrich P8833-25G) selection starting on day 1 ...
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bioRxiv - Cell Biology 2024Quote: ... Transfer dissected nTS into dounce which includes 1 mL DB with 1 mM DTT (D9779, Sigma), 1x cOmplete EDTA-free protease inhibitor (05056489001 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were further washed after 3 days and the medium changed to RPMI supplemented with 10% heat-inactivated human serum (Sigma). MDM were then infected 3-4 days later at low multiplicity of infection ...
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bioRxiv - Bioengineering 2022Quote: ... All primary cells were used between subculture 3–7 and were routinely cryopreserved in 90% human serum (#H4522; Sigma-Aldrich) supplemented with 10% dimethyl sulfoxide (DMSO).
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bioRxiv - Immunology 2020Quote: Primary human NK cells were incubated after enrichment for 16 hours at 37°C in 3 ng/mL IL-15 (Miltenyi) in RPMI media supplemented with 10% Human AB serum (Sigma). Cells were washed with PBS ...
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bioRxiv - Immunology 2021Quote: ... and PBS-treated (n ≥ 3) control human gut xenografts and mouse jejunum were purified using GenElute Mammalian Total RNA kit (Sigma) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... followed by streptavidin-Cyanine-3 (1:500; Amersham Bioscience) or by goat anti-human IgG coupled to FITC (1:100, Sigma), as appropriate ...
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bioRxiv - Biochemistry 2021Quote: Human IgM purified from human serum (Sigma) was digested with trypsin ...
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bioRxiv - Cancer Biology 2021Quote: ... siECHS1 (SASI_Hs01_00085563 and SASI_Hs02_00336896) and the negative control si-RNA (NT-siRNA; SIC001) were purchased from Sigma-Aldrich. Plasmid encoding HA-NRF2 (pLV-mCherry-CMV HA-NRF2 ...
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bioRxiv - Microbiology 2021Quote: ... Putative ligand proteins (elastin from human skin, fibrinogen from human plasma, laminin from human placenta, fibronectin from human plasma) (all from Sigma) were resuspended in carbonate-bicarbonate buffer (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Calu-3 cells (a human lung epithelial cell line; ATCC HTB-55) were maintained in EMEM (Sigma-Aldrich, Cat# M4655-500ML) containing 20% FBS and 1% PS ...
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bioRxiv - Microbiology 2023Quote: ... the wells were filled with 200 µl fresh M63 media with or without 3 µM of human transferrin (Sigma-Aldrich #T1147) and incubated at room temperature for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 500k Day 25 hiPSC-CMs and 100k human Hs27a stromal cells were mixed with 3 U/mL thrombin from bovine plasma (Sigma, T4648) and 5 mg/mL bovine fibrinogen (Sigma ...
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bioRxiv - Cell Biology 2023Quote: The MISSION® pLKO.1-puro Non-Target shRNA (scr) and the shRNA against the UTR of human ZIP11 gene (5’-TCCTGATTGACTCTGATTATA-3’, Cat. TRCN0000434903) were from Sigma-Aldrich. The mammalian gene expression vector pLV[Exp]-EGFP/Neo-Ef1A (pLV ...
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bioRxiv - Immunology 2020Quote: ... or human (1:20000; anti-human IgG; Sigma) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... 3 methylcholanthrene (3-MC; Sigma-Aldrich), contained in DMEM with a final concentration of 0.1% of dimethylsulfoxide (DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... 3-deazauridine (3-DU) (Sigma Aldrich) was used as a non-selective inhibitor of CTPS1 and CTPS2.
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bioRxiv - Immunology 2019Quote: ... for 6 hours and then exposed to 0.5 × 105 red and 0.5 × 105 blue opsinised (1 hour in 10%v/v human serum) 3 μm diameter latex polystyrene beads (Sigma-Aldrich, Gilligham, UK) such that there were 1 bead per cell ...
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bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... 3 mM MgCl2 (Sigma, 7786-30-3), 0.1% Tween-20 (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... and EIF4E2 shRNA#3 (sh4EHP#3) (Sigma,). GIGYF2 shRNA#1 (shGIGYF2#1 ...
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bioRxiv - Cancer Biology 2022Quote: ... covered with 3-aminopropyltrimethoxysilane (3-APTS, Sigma) for 3 min for activation ...
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bioRxiv - Neuroscience 2021Quote: ... oxygenated DAB (3-3’-diaminobenzidine, Sigma-Aldrich) was dissolved in 0.1 N HCl at a concentration of 5.4 mg/ml and subsequently diluted ten-fold into sodium cacodylate buffer (pH 7.4 ...
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bioRxiv - Bioengineering 2022Quote: ... 3-mercaptobenzoic acid (3-MBA, Sigma-Aldrich)-capped gold nanoclusters (3-MBA-Au nanoclusters ...
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bioRxiv - Physiology 2022Quote: ... with 37 °C modified Tyrode (NT) solution (containing, in mM: 120 NaCl, 4.7 KCl, 1 MgCl2, 1.8 CaCl2, 10 glucose, 10 HEPES [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2023Quote: ... The indicated 119-nt single-stranded oligodeoxynucleotide (ssODN) donor template for homology-directed repair (HDR) was custom prepared (Sigma) and resuspended in TE buffer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... or human liver microsomes (HLMs) (male human pooled, Sigma-Aldrich), respectively ...
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bioRxiv - Cell Biology 2019Quote: ... animals were boosted 5 times in a 3 week-interval with 0.2 mg human TNFa in incomplete adjuvant (Sigma Chemical, St. Louis, MO). Final boost was given intravenously with 0.4 mg the protein in PBS four days before splenectomy ...
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bioRxiv - Cancer Biology 2020Quote: ... Human haemoglobin (Sigma) was used for creation of standard curve.
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bioRxiv - Microbiology 2021Quote: ... human insulin (Sigma), 10’000 units/ml of penicillin and 10’000 µg/ml streptomycin (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... human HDL (Millipore), human LDLs (Millipore ...