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Citations for Millipore Sigma :
151 - 200 of 3488 citations for NGAL Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... and purified on His•Bind resin (Novagen) in the presence of 8 M urea according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... anti-His monoclonal antibody (Sigma, 1:1000) and anti-mouse (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with His-HDAC6 (EMD Millipore) and 1 mM ATP in kinase buffer for 1 h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... then detected with anti-His antibody (Sigma). S protein expression was detected with anti-SARS-CoV-2 S protein S2 subunit mouse mAb unconjugated (GeneTex) ...
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bioRxiv - Immunology 2020Quote: ... with added 10% HI FBS (Sigma-Aldrich) and 1% Pen/Strep (Lonza) ...
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bioRxiv - Cell Biology 2021Quote: ... or anti-HIS (1:10,000, Sigma H1029) was used ...
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bioRxiv - Neuroscience 2021Quote: ... amino acids (His, Ser, Ala, Trp; Sigma) were diluted to a final concentration of 10-4 M and bile acid (TDCA ...
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bioRxiv - Microbiology 2023Quote: ... anti-His (mouse, 1:3k, Sigma, A7058), anti-c-Myc (mouse ...
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bioRxiv - Biochemistry 2022Quote: ... anti-His (Millipore: 05-949, clone HIS.H8) or Goat anti-Mouse IgG (Millipore ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and His-Bind Purification kit (Novagen, USA) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... L-histidine (L-his, Sigma-Aldrich #H8125), L-ornithine (L-orn ...
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bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
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bioRxiv - Neuroscience 2019Quote: ... the transfected HEK 293T cells were treated with MG132 (Sigma-Aldrich) at a concentration of 20 μM for 16 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK-293T were grown in Hepes-modified DMEM (Sigma-Aldrich, D6171), supplemented with 10% FCS (Eurobio Scientific ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged recombinant proteins were detected using an HRP-conjugated anti-His monoclonal antibody (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... His- and GST-tagged proteins were detected with anti-His (Monoclonal Anti-polyHistidine antibody, Sigma H1029, 1:5000 dilution) or anti-GST (Mouse anti-GST monoclonal antibody ...
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bioRxiv - Microbiology 2019Quote: ... were cultured in suspension in serum-free Ex-Cell 293 medium (Sigma) and Expi293 medium (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... HEK293E cells were cultured in EX-CELL 293 serum-free medium (Sigma) and incubated with agitation at 37°C and 4.5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 293 cells were cultured in MEM medium (Sigma-Aldrich, St. Louis USA) supplemented with 10% (v/v ...
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was eluted in a gradient of His-AC washing buffer and His-AC elution buffer (both supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...
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bioRxiv - Microbiology 2020Quote: ... as a His-tag fusion in pET28b (Novagen). Cells were grown in Terrific-Broth at 37°C to an OD600 = 1.2 and after 15 min at 4°C induced with 1 mM isopropyl β-d-thiogalactoside ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected using anti-His-HRP (Sigma) and anti-FLAG-HRP (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... -His with or without 3-AT (Sigma-Aldrich).
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bioRxiv - Pathology 2021Quote: ... cloned into a pET28a His-tag vector (Novagen) and expressed in E ...
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bioRxiv - Microbiology 2020Quote: ... Mouse anti-His antibody (1:12000) (Sigma-Aldrich) and biotinylated PNA (1:6000 ...
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bioRxiv - Biophysics 2020Quote: ... His-purifying resin was from Novagen (Darmstadt, Germany). Bis-(sulfosuccinimidyl ...
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bioRxiv - Cell Biology 2021Quote: ... and Anti-His(1:3000) antibody(Sigma #H1029).HRP conjugated mouse IgG was used at 1:4000 dilution(Sigma #A3673 ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (Sigma H1029; 1:3000 for IB), anti-Hsp70 (Abcam ab47455 ...
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bioRxiv - Plant Biology 2022Quote: ... followed by blotting using Anti-His (SAB4301134, Sigma) and Anti-GST antibody (ab9085 ...
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bioRxiv - Genomics 2023Quote: ... with 10% heat-inactivated (HI) FBS (Sigma F0926). Vero/hSLAM cells were a kind gift from Dr ...
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bioRxiv - Systems Biology 2023Quote: ... MCF7 in DMEM-Hi Glucose medium (Sigma-Aldrich) HCC1937 and ZR-75-1 in RPMI 1640 media (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 2% GlutaMAX (200 mM ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-His-primary antibody (Sigma, 1:2,000) was incubated with the protein-treated lipid strips to detect Jps1 bound via its C-terminal His-tag ...
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bioRxiv - Cell Biology 2024Quote: ... +10% HI FBS(Sigma Aldrich, cat#F4135-500ML) + penicillin/streptomycin (Sigma ...
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bioRxiv - Immunology 2019Quote: ... HEK-293T cells were plated on poly-L-Lysine-coated (Sigma; P4707) 10 cm plates (Corning ...
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bioRxiv - Cell Biology 2022Quote: BEAS-2B and HEK-293T cells (ATCC) were grown in DMEM (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: HEK 293T cells were cultured in DMEM with 10% FBS (Millipore Sigma), GlutaMAX (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... HCC5044 and HEK-293T cells were cultured in DMEM (Sigma-Aldrich, D5796) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2023Quote: ... TPC-1 and HEK 293T/17 were cultured in DMEM (Sigma D6429) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Bioengineering 2024Quote: ... typically in an equal mixture of Expi293 and EX-CELL 293 (14571C Sigma). Expi293 media can be used for the entire process ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...