Labshake search
Citations for Millipore Sigma :
601 - 650 of 10000+ citations for Mouse Protein Jagged 2 JAG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... Gfp-tagged protein of interest was detected using a primary mouse anti-Gfp (1:3,000, Millipore/Sigma). An anti-mouse IgG-horseradish peroxidase (HRP ...
-
bioRxiv - Immunology 2022Quote: ... the DuolinkTM InSitu Orange Starter Kit Mouse/Rabbit (DUO92102) from Sigma Aldrich was used ...
-
bioRxiv - Developmental Biology 2019Quote: ... Genotyping was performed using the KAPA HotStart Mouse Genotyping Kit (Sigma, KK7352) using GFP primers ...
-
bioRxiv - Cancer Biology 2021Quote: ... The rat/mouse insulin kit (EZRMI-13K) was purchased from EMD Millipore Corporation ...
-
bioRxiv - Developmental Biology 2022Quote: ... Genotyping was performed using the KAPA HotStart Mouse Genotyping Kit (Sigma, KK7352) using primers listed in Supplementary Table 2 ...
-
bioRxiv - Cancer Biology 2020Quote: The DuoLink® In Situ Red Starter Kit Mouse/Rabbit (Sigma-Aldrich) was used to detect proximity between FXR1 protein and cMYC mRNA according to manufacturer protocol ...
-
bioRxiv - Microbiology 2020Quote: ... using Milliplex Mouse Cytokine/Chemokine MAGNETIC BEAD Premixed 25 Plex Kit (Millipore), according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2021Quote: ... and mouse insulin RIA kit (Cat#SRI-13K, Millipore, Billerica, Massachusetts, USA) was used to measure plasma insulin ...
-
bioRxiv - Physiology 2020Quote: ... we performed PLAs using the Duolink® Starter Kit Mouse/Rabbit (Sigma) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Liver triglyceride and total cholesterol concentrations were measured using commercial kits (all from Instruchemie) and expressed as nanomoles per milligram of total protein content using the Bradford protein assay kit (Sigma-Aldrich).
-
bioRxiv - Microbiology 2020Quote: ... pLysS cells expressing His-topoIB and T18 tagged genome segregation proteins in different combinations using the kit Protein G immunoprecipitation from Sigma-Aldrich as described previously (Maurya et ...
-
bioRxiv - Microbiology 2020Quote: Protein concentration was determined by the bicinchoninic acid protein assay kit (BCA-Pierce, USA) using bovine serum albumin (BSA, Sigma-Aldrich) as standard.
-
bioRxiv - Immunology 2021Quote: ... The protein concentration in the final samples was measured with the use of Bicinchoninic Acid Kit for Protein Determination (Sigma-Aldrich) and a 96-well plate analyzer (Tecan Infinite 200 PRO).
-
bioRxiv - Developmental Biology 2023Quote: RNA-Binding protein immunoprecipitation (RIP) for PCBP1 was perform using Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, cat 17-700) and the same PCBP1 antibody used for eCLIP-seq (Abcam cat# ab168377) ...
-
bioRxiv - Neuroscience 2023Quote: ... The ATP level for each sample was normalized to the protein content of each sample using the BCA protein assay kit (Sigma-Aldrich). Results are expressed as fold change ± S.E.M ...
-
bioRxiv - Molecular Biology 2024Quote: The proximity ligation assay (PLA) was conducted to determine potential protein–protein interactions using the Duolink in situ detection kit (Sigma-Aldrich) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... 167 mM NaCl) and precleared for 2 h with 250 μL of previously blocked 50% protein-A and protein-G beads (Sigma P-7786 and P-3296 respectively). Blocking was achieved by incubating the beads with 0.5 mg/ml of Ultrapure BSA and 0.2 mg/mL of salmon sperm DNA for 4 h at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... For p65 IP the lysates were then incubated with 2 μg of antibody CST ref #6956 or CST ref# 8242) at 4 °C for 2 hrs before adding 20 μL of slurry protein G beads (Sigma-Aldrich Fast Flow Protein G sepharose) for 20 min ...
-
bioRxiv - Cell Biology 2023Quote: PLA assays were carried out using a Duolink in situ red starter kit mouse/rabbit kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
-
bioRxiv - Physiology 2022Quote: The mNGF level in tears upon a AAV2/9 CAG-mNGF injection in the LG was dosed using the mNGF ELISA kit (Sigma-Aldrich, reference RAB1119, France) following the manufacturer’s recommendations ...
-
bioRxiv - Neuroscience 2020Quote: ... ELISA reaction procedure was followed as described by manufacturer (OPD; Sigma). The optical density of the supernatant was read on a spectrophotometer at 492 nm ...
-
bioRxiv - Neuroscience 2022Quote: ... Multiplex ELISA was performed on a Luminex FlexMAP 3D (Millipore Sigma) using a premixed 32-plex cytokine magnetic bead panel (Millipore Sigma ...
-
bioRxiv - Physiology 2021Quote: ... 100 μl of 3,3’,5,5’-tetramethylbenzidine (TMB) Elisa substrate (T0440; Sigma) was added and the reaction stopped after 10 min using 2 M H2SO4 ...
-
bioRxiv - Neuroscience 2023Quote: ... Leptin concentrations were determined by ELISA (Millipore, St. Charles, MI, USA). The blood glucose levels were measured using a Glucocard glucometer (Arkray ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... Cytokines and CCL5 were measured by Multiplex ELISA (MCYTOMAG-70K, Millipore) or Enzyme Linked Immunosorbent Assay (ELISA ...
-
bioRxiv - Immunology 2023Quote: ... All ELISA assays were developed using TMB liquid substrate system (Sigma), stopped with 2N sulfuric acid ...
-
bioRxiv - Microbiology 2023Quote: Nunc MaxiSorp flat bottom 96-well ELISA plates (Sigma-Aldrich, M9410) were coated with 50 ng/well of recombinant CCHFV Gn protein (Creative Diagnostics ...
-
bioRxiv - Genetics 2020Quote: ... FLAG-tagged LIR-2 protein was detected with monoclonal anti-FLAG M2-Peroxidase (HRP) antibody (SIGMA-Aldrich) and EGFP:NHR-25 was visualized with Clarity Western ECL substrate (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
-
bioRxiv - Physiology 2019Quote: ... Proteins were resuspended in concentrated urea buffer (8 M Urea, 2 M Thiourea, 150 mM NaCl (Sigma)) ...
-
bioRxiv - Cancer Biology 2020Quote: ... proteins were eluted twice with 2 ug/uL V5 peptide in water (Sigma-Aldrich, St. Louis, MO) at 37°C for 15 minutes with shaking at 1000rpm ...
-
bioRxiv - Plant Biology 2020Quote: ... 1 μg fibrillarin 2 or NOP56 antibody was coupled to 30 μL protein-A agarose beads (Sigma) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... 2 µl of the supernatant were used for protein quantification using the Direct Detect system (Merck Millipore).
-
bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
-
bioRxiv - Cancer Biology 2019Quote: ... The lyophilized proteins were resuspended in 1 ml of a 2 mg/ml DTT (1,4-Dithiothreitol, Sigma) solution and incubated at 50C for 2h ...
-
bioRxiv - Molecular Biology 2020Quote: ... 3 mg total protein were mixed with 2 mL Urea Buffer (UB) (8 M urea (Sigma, U5128) in 0.1 M Tris/HCl pH 8.0 and 50mM ammonium bicarbonate) ...
-
bioRxiv - Neuroscience 2019Quote: ... Eya1 protein was concentrated to approximately 2 mg/ml using an Amicon Ultra concentrator (30 MWCO, Millipore) and further purified by size-exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Lifescience ...
-
bioRxiv - Biochemistry 2021Quote: ... protein lysates from the single cells were digested with 2 ng of Trypsin (Sigma cat. nr. T6567), dissolved in 1 μl of 100 mM TEAB pH 8.5 containing Benzonase (Sigma cat ...
-
bioRxiv - Immunology 2020Quote: ... Detection of the SARS CoV-2 spike protein was achieved by incubation with an anti-rabbit (Sigma) secondary antibody ...
-
bioRxiv - Molecular Biology 2021Quote: ... (Table 2) and the immune complexes were captured with protein A agarose fast-flow beads (Sigma-Aldrich) for 1h at RT ...
-
bioRxiv - Systems Biology 2021Quote: ... protein lysates from the single cells were digested with 2 ng of Trypsin (Sigma cat. nr. T6567), dissolved in 1 μl of 100mM TEAB pH 8.5 containing Benzonase (Sigma cat ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein was reduced with a final concentration of 10 mM Tris(2 - carboxyethyl)phosphine hydrochloride (TCEP) (Sigma) and alkylated with 40 mM 2-chloroacetamide (CAA ...
-
bioRxiv - Molecular Biology 2022Quote: ... the proteins were eluted by 2 mM biotin in buffer W and concentrated with 100kDa Amicon (Millipore). The concentration of dtSARM1 was determined by BCA assay (Thermo Fisher).
-
bioRxiv - Plant Biology 2023Quote: ... Labelled proteins were biotinylated with 20 μM azide-PEG3-biotin (Sigma-Aldrich, 2 mM stock in DMSO) using click chemistry as described in “Labeling and click chemistry” ...
-
A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... 2 mgs of total protein were incubated with 20 μL of V5 agarose (Sigma-Aldrich, Catalog # 7345) or FLAG M2 resin (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... total proteins were loaded in the Laemmli sample buffer supplemented with 5% 2-mercaptoethanol (Sigma, catalog #: M3148) to reduce the disulfide bonds ...
-
bioRxiv - Plant Biology 2024Quote: ... Labelled proteins were biotinylated with 30 μM azide-PEG3-biotin (Sigma-Aldrich, 2 mM stock in DMSO) using click chemistry as described above ...
-
bioRxiv - Microbiology 2020Quote: ... The proteins were then visualized with ProteoSilver Plus Silver Stain Kit (Sigma Aldrich) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2019Quote: ... and the protein concentration was assessed using a BCA kit (Sigma-Aldrich, USA). Samples were diluted to equal concentrations and separated using the Novex Native PAGE Bis–Tris Gel system (4–16% ...
-
bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were determined using the BCA method (QuantiPro assay kit; Sigma-Aldrich).