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Citations for Millipore Sigma :
701 - 750 of 10000+ citations for Mouse DEFB15 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... laboratory protocol “Lentivirus production of shRNA or ORF-pLX clones,” and single clones were isolated following puromycin selection (2 μg/mL; Sigma-Aldrich #P8833). RNA-seq data were aligned with STAR to mouse reference genome mm10 (GRCm38) ...
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bioRxiv - Microbiology 2024Quote: ... TRCN0000148694) and a control scrambled shRNA pLKO.1 clone (SHC-002) were performed using MISSION Lentiviral Packaging Mix (Sigma-Aldrich, Diegem, Belgium) and FuGENE HD (Promega ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-mouse αSMA (Sigma A2547), goat-anti-human TIE1 (R&D AF619) ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid DNA was extracted from bacteria using GenElute™ Plasmid Miniprep Kit (Sigma Aldrich), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... we transfected lentiviral plasmids with packaging plasmids in 293T cells using polyethylenimine (Sigma, 408727), harvested supernatants containing viral particles 48 and 72 hours later ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were prepared using the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit (NA0410; Sigma). Silica fines in the final product were carefully eliminated by additional centrifugation at 12,000 g for 30 min.
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were extracted with the Gene Elute Plasmid Miniprep kit (Sigma, St. Louis, USA) and sent for sequencing to two different companies (EuroFins Genomics ...
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bioRxiv - Molecular Biology 2019Quote: ... The plasmid pET-28a (+) (Novagen) carries an N-terminal His-Tag/thrombin/T7 configuration ...
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bioRxiv - Cancer Biology 2020Quote: ... ShScr and shIDO plasmids (Sigma)[35] were electroporated into ST strains using a BTX electroporator (1mm gap cuvettes ...
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bioRxiv - Biochemistry 2020Quote: ... carrying the pRARE2 plasmid (Novagen) and initially purified using the same protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The pRSFDuet-1 plasmids (Novagen) encoding SARS-CoV-2 nsp14 or nsp10 engineered with an N-terminal His-SUMO tag were prepared as follows ...
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bioRxiv - Microbiology 2021Quote: ... plasmids and primers (Sigma, Singapore) used in this study are listed in S6 and S7 Tables ...
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bioRxiv - Synthetic Biology 2023Quote: The pET-11a plasmid (Novagen) was used as the genetic vector to host all three components constituting our genetic circuit ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasmids were purchased from Sigma.
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bioRxiv - Genetics 2023Quote: ... harboring the pRARE plasmid (Novagen). Super Broth media (3 L ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plasmid pET-19b (Novagen) was double digested using NcoI and BamHI and Gibson assembly performed to insert a gene construct into the plasmid ...
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bioRxiv - Immunology 2021Quote: ... Expression of PTSN was silenced in U2-OS and 293A cells by lentiviral transduction of a pLKO1 shRNA against both β- and α-PTSN (TRCN0000282572, Sigma-Aldrich). pLKO1 shRNA (SHC016V ...
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bioRxiv - Microbiology 2019Quote: ... The Isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible lentiviral backbone pLKO-puro-IPTG_3xLacO harboring the non-mammalian shRNA control was purchased (Mission™SHC332; Sigma Aldrich, Buchs, Switzerland). The puromycin resistance gene was replaced via restriction digestion cloning using a PCR-amplified fluorescent mCherry marker containing overhangs with compatible restriction sites to generate the pLKO-mCherry_IPTG_3xLacO lentiviral backbone.
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bioRxiv - Cancer Biology 2019Quote: ... for primary cells miR-E shRNA viruses were concentrated to increase transduction efficiency using Centriprep Centrifugal filter YM-50 (Millipore, Billerica, MA, USA).
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bioRxiv - Physiology 2021Quote: ... The shRNA-containing vector was co-transfected with the pE-ampho vector into HEK293T cells using GeneJuice transfection reagent (Merck Millipore, Burlington, MA, USA). Supernatants containing viral particles were collected 48 h after the transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... HT-29/19A and HCT-116 cells were transduced with lentivirus expressing short-hairpin RNAs (shRNAs) targeting fascin1 (Sigma-Aldrich, St. Louis, MO). For the rescue experiments ...
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bioRxiv - Immunology 2022Quote: Lentiviral plasmids encoding shRNA for gene knockdown for PRDM1-L or open reading frame of overexpression for PRDM1-S and PRDM1-L were obtained from Sigma-Aldrich (MISSION shRNA) and Horizon Discovery Biosciences (Precision LentiORF) ...
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bioRxiv - Cell Biology 2023Quote: U2Os-AP2-GFP cells were transduced with lentiviral media expressing respective shRNAs in the presence of Polybren 8 μg/ml (Sigma-Aldrich, TR-1003) for 5 hours ...
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bioRxiv - Neuroscience 2023Quote: Knockdown of proteins was achieved through RNA interference either by CaCl2 transfection of gene-specific shRNA encoding vectors (pLKO.1; Sigma-Aldrich or shRNAmiR) on DIV 7 or by transduction of cells with lentiviral particles harboring the gene-specific shRNA on DIV 2 ...
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bioRxiv - Immunology 2024Quote: ... Two different Mission lentivirus-based plasmids of shRNAs (clone numbers TRCN0000123050 and TRCN0000436778) against human ZBP1 and the shcontrol vector TRC2 pLKO.5-puro nonmammalian shRNA (SHC202) were obtained from Sigma-Aldrich (Burlington, MA). 293T cells were cotransfected with the shRNA and packaging plasmids psPAX2 and pMD2 using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... Cy3-mouse-anti-mouse aSMA (Sigma C6198), mouse anti-mouse αSMA (Sigma A2547) ...
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bioRxiv - Cancer Biology 2020Quote: ... plasmids were isolated from selected library cells using the Montage Plasmid MiniprepHTS Kit (MilliPore, LSKP09604) on a MultiscreenHTS Vacuum Manifold (MilliPore ...
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bioRxiv - Immunology 2022Quote: ... Plasmid was purified from transformed E.coli using Genelute Endotoxin-free plasmid midiprep kit (Sigma-Aldrich) per manufacturers’ instructions and sequenced to confirm insert fidelity and orientation ...
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bioRxiv - Cancer Biology 2023Quote: Both wild-type and mutant DDX3X cDNA plasmids were transfected in the shRNA (shUTR) knockdown polyclonal population of Daoy cells using the X-tremeGENE™ HP DNA transfection reagent (Sigma-Aldrich, MO, USA). Expression of the exogenous DDX3X was checked by western blotting using an anti-FLAG® M2 antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... cell lines with stable expression of KCNN4 shRNAs were generated by selection in medium containing puromycin at a concentration of 1 μg/mL (Sigma-Aldrich, St Louis, USA).
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bioRxiv - Cancer Biology 2020Quote: ... Anti-mouse agarose or mouse agarose (both Sigma) were added for 1 hour at 40C prior to 3 washes in Lysis Buffer ...
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bioRxiv - Neuroscience 2019Quote: ... Mouse anti-mouse NeuN (Millipore, MAB377, 1:100), and Rabbit Anti-MBP (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... and Mouse-anti-mouse/rat-NeuN (1;200, Millipore). Secondary antibodies include Goat-anti-Chicken-IgG-488 (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... These confirmed colonies underwent plasmid prep using the GenElute™ Plasmid Miniprep Kit (Sigma-Aldrich, Australia) and the complement-containing plasmid was electroporated into the knockout mutant as per the protocol above.
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bioRxiv - Cancer Biology 2020Quote: ... For lentivirus production we transfected lentiviral plasmids with packaging plasmids in 293T cells using polyethylenimine (Sigma), harvested supernatants containing viral particles 48 and 72 hours later ...
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bioRxiv - Systems Biology 2021Quote: ... Plasmid isolation was done with the GenElute™ Plasmid Miniprep Kit (Sigma-Aldrich, St. Louis, MO). Yeast transformations were performed according to the lithium acetate method [111].
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bioRxiv - Plant Biology 2021Quote: The plasmids pET-mSAV and pET-mSAV-pep4 were generated based on pET-plasmid (Novagen, USA) and contained sequence coding for mature streptavidinstreptavidine (SAV ...
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bioRxiv - Bioengineering 2022Quote: ... The plasmid pET28a(+) (Novagen, Darmstadt, Germany) was used as the expression vector ...
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bioRxiv - Cancer Biology 2021Quote: ... For inducible Myc knockdown plasmid (Sigma Mission shRNA library ...
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bioRxiv - Cell Biology 2022Quote: ... coli using plasmid preparation kits (Sigma) and sequence verified by Sanger sequencing (QuintaraBio).
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bioRxiv - Synthetic Biology 2020Quote: ... using plasmid pTrc99a (Novagen, CA, USA) as the template along with the er-Trc F and er-Trc R ...
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bioRxiv - Molecular Biology 2019Quote: ... 68 The pET21a plasmid backbone (Novagen) was used to drive the expression of a hexa-His-tagged version of each protein ...
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bioRxiv - Neuroscience 2021Quote: ... plasmid construct in DMEM:F12 (Sigma, D6421) or neurobasal medium (primary cortical neurons only) ...
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bioRxiv - Molecular Biology 2021Quote: ... T7 blue plasmid (Novagen, Merck Millipore) and human genomic DNA (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2021Quote: ... T7 blue plasmid (Novagen, Merck Millipore) and human genomic DNA (Roche Diagnostics ...
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bioRxiv - Bioengineering 2022Quote: ... using the plasmid pIEX (Merck Millipore) with a MB7 signal sequence ...
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bioRxiv - Biochemistry 2022Quote: ... coli expression plasmid pET-15b (Novagen), using these same sites ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10.7μg plasmid DNA (Sigma Aldrich; MX1 (#3 ...
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bioRxiv - Microbiology 2023Quote: ... the plasmid pGEX-2TK (Sigma-Aldrich) was digested with SmaI ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were obtained from Millipore Sigma (shzDHHC5 andshzDHHC21 in pLKO.1 vectors provided as bacterial glycerol stocks ...