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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for Mouse Antigen peptide transporter 2 TAP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... mouse α-tubulin (1:10,000, B-5-1-2, Sigma Aldrich). Secondary HRP-conjugated antibodies (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... stimulated with SARS-CoV-2 spike protein overlapping peptide library or stimulated with 5μg/ml of Phytohaemagglutinin (PHA) (Sigma-Aldrich) as a positive control ...
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bioRxiv - Molecular Biology 2022Quote: ... Dynabeads following the first pulldown were resuspended in Re-ChIP elution buffer (20 mM Tris-Cl pH 7.5, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 100 µg of 3X FLAG peptide [Sigma, #F4799]), and incubated in 4 °C for 1 hour with rotation to selectively elute FLAG-fusion protein ...
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bioRxiv - Immunology 2020Quote: ... A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 µg/mL final concentration per peptide) or controls (gp70-AH1 [SPSYVYHQF]38, 4 µg/mL; concanavalin A [ConA], 2 µg/mL [Sigma]). Streptavidin-alkaline phosphatase (ALP ...
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bioRxiv - Immunology 2020Quote: ... A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 μg/mL final concentration per peptide) or controls (gp70-AH1 [SPSYVYHQF]33, 4 μg/mL; Concanavalin A [ConA], 2 μg/mL [Sigma]). Streptavidin-alkaline phosphatase (ALP ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EDTA and 2 mM PMSF) and eluted with 1 mg/ml FLAG® Peptide (Sigma, cat. no. F3290) in wash buffer overnight on a rotator at 4°C for anaylsis by mass spectrometry.
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bioRxiv - Neuroscience 2022Quote: ... peptides were acidified with 2 μl of 1% TFA then desalted using C18 ZipTips® (Millipore, cat no. Z720070-96EA).
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bioRxiv - Genetics 2020Quote: ... Specific primers for mouse leptin and 2 mouse housekeeping genes used for normalization (β-actin and 34B4 mouse genes) were purchased from Sigma (Sigma, France). We used primers for Leptin (forward ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 anti-RBD and N IgG antibody levels were determined using the Milliplex® SARS-CoV-2 antigen panel 1 IgG assay (Millipore Sigma, Cat No. HC19SERG1-85K) with the Wuhan-1 strain RBD and N proteins included ...
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bioRxiv - Immunology 2023Quote: ... Each group (n = 6) was immunized intraperitoneally with peptides-KLH (10 μg/mouse) emulsified in Incomplete Freund’s Adjuvant (Sigma, Cat.#F5506), and a control group (n = 6 ...
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bioRxiv - Immunology 2020Quote: ... Total IgG response was evaluated by enzyme-linked immunosorbent assays (ELISAs) using 1:5000 dilution of HRP conjugated anti mouse secondary antibody (A4416, Sigma-Aldrich, USA).
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bioRxiv - Cell Biology 2021Quote: ... Insulin secreted from the islet grafts was measured in the mouse serum three weeks post-transplant using a human insulin ELISA (Millipore, EZHI-14K).
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bioRxiv - Physiology 2021Quote: Cortisol concentrations were measured in mouse sera by a solid phase competitive enzyme linked immunosorbent assay (ELISA) (Sigma-Aldrich Co Ltd, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: IgG mediated antibody titer of serum samples collected from immunized animals and convalescent patients’ sera were determined by enzyme-linked immunosorbent assay (ELISA)35 using 1:5000 dilution of HRP conjugated anti mouse (A4416, Sigma-Aldrich, USA) and 1:10000 dilution of HRP conjugated anti human secondary antibody (A0170 ...
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bioRxiv - Neuroscience 2021Quote: ... C-reactive protein (CRP) was measured by an ELISA kit (cat. number CYT294, Millipore Sigma, Saint Louis, MO) following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... also contained 1-2 µg/uL α-MBPAF546 or 1-2 µg/uL mouse α-BRCA2 (Ab1, Millipore) plus 1-2 µg/µL goat α-mouse IgGAF546 (Molecular Probes).
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bioRxiv - Neuroscience 2019Quote: ... Synaptic puncta were identified by the co-localization of pre-synaptic Vesicular Acetylcholine Transporter (VAChT) protein and the post-synaptic Shank protein in MAP2 stained neurons using rb anti-VAChT (Sigma Aldrich; 1:1000), ms anti-shank (Neuromab ...
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bioRxiv - Developmental Biology 2019Quote: ... Sections were also stained with antibodies against the syncytiotrophoblast layer II marker monocarboxylate transporter 4 (MCT4; Merck Millipore AB3314P, used at 1:100) and against E-cadherin (CDH1 ...
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bioRxiv - Immunology 2021Quote: Synthetic peptide analogues for validation targets were analysed together with MSRT retention time peptides standards (Sigma) by LC-MS/MS using an Orbitrap Fusion Lumos mass spectrometer ...
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bioRxiv - Cancer Biology 2019Quote: ... Resultant peptides were further concentrated and 3 × FLAG peptide removed using Amicon centrifugal filtration devices (Millipore).
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bioRxiv - Pathology 2019Quote: ... a Milliplex mouse cytokine magnetic kit (Millipore, France) was used according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... A Milliplex Mouse Th17 Luminex kit (EMD Millipore) with analytes IL-1β ...
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bioRxiv - Immunology 2021Quote: ... A Milliplex Mouse Th17 Luminex kit (MD MilliPore) with analytes IL-1β ...
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bioRxiv - Biophysics 2019Quote: ... Peptide concentrations (Direct Detect spectrophotometer, Millipore) in injection buffer (5% HPLC grade acetonitrile ...
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bioRxiv - Immunology 2021Quote: ... Peptide (ELAGIGILTV, purchased from Sigma-Aldrich) pulsing was performed in serum-free medium ...
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bioRxiv - Microbiology 2021Quote: ... or HA peptide (Sigma, cat# I2149), respectively.
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bioRxiv - Molecular Biology 2020Quote: ... 0.4 mg/ml 3xFLAG peptide (Sigma)) totaling 1 hr ...
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bioRxiv - Biochemistry 2019Quote: ... and 3× FLAG peptide (Sigma-Aldrich). The eluate was concentrated by centrifugation using an Amicon Ultra (Merck ...
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bioRxiv - Neuroscience 2021Quote: ... anti-vasointestinal peptide (VIP, Millipore, Rabbit) at concentrations suggested by the provider.
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bioRxiv - Plant Biology 2022Quote: ... 500μg/ml 3x FLAG peptide (Millipore)] for FLAG-IP samples or ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG® Peptide (Sigma-Aldrich, F3290), Protein A agarose (Pierce ...
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bioRxiv - Microbiology 2024Quote: ... and eluted with 3XFLAG peptide (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 µg/ml 3xFlag peptide (SIGMA), 0.1 mM PMSF and PI tablet]46.
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bioRxiv - Biochemistry 2020Quote: ... For ELISAs with asialofetuin (Sigma Aldrich # A4781), 5 μg/mL was immobilized ...
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bioRxiv - Physiology 2023Quote: ... Insulin was measured by ELISA (Millipore Sigma). For glucose tolerance tests ...
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bioRxiv - Bioengineering 2020Quote: ... Bound proteins were eluted by competition with four 2 x CV of DPBS containing 100 µg/mL FLAG peptide (Sigma Aldrich). Purified proteins were formulated in DPBS by buffer exchange through desalting columns (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... 2% glycerol) and then eluting by shaking with 15 μL of 100 μg/μL of 3X FLAG peptide (Sigma-Aldrich, F4799) in the original lysis buffer for 30 min at 4°C.
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bioRxiv - Cell Biology 2023Quote: ... and SKR-2-containing protein complexes were eluted overnight in 100 µl of the lysis buffer containing 2 mg/mL of 3xFlag peptide (Sigma F4799). Eluted protein samples were digested with trypsin and analyzed by MudPIT in the Mass Spectrometry and Proteomics Facility at Johns Hopkins School of Medicine.
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bioRxiv - Immunology 2022Quote: ... IL-10 and TNF-α were determined in 24 h culture supernatant of UNT control and treated MOs using respective ELISA kits (Sigma-Aldrich kit, USA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... antigen retrieval was performed in 1% citrate buffer (Sigma-Aldrich, Madrid, Spain) and 0.05% of Tween 20 (Sigma-Aldrich ...
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bioRxiv - Pathology 2020Quote: ... Enzymatic antigen retrieval was performed using α-chymotrypsin (Sigma-Aldrich, MO, USA) for 10 min at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit polyclonal antibody against the human ZCCHC10 antigen (HPA038944, 1:2,000; Sigma), mouse monoclonal antibody against the FLAG antigen (F1804 ...
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bioRxiv - Cancer Biology 2020Quote: ... and non-specific antigens were blocked with 10% goat serum (Sigma, UK). CD15 and CD15s primary antibodies were applied for one hour followed by incubation in secondary conjugates for 30 minutes ...
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bioRxiv - Physiology 2020Quote: ... Antigen retrieval was performed with 10 mM Sodium Citrate buffer (Sigma Aldrich) at pH=6-6.2 for 15 min at 95°C ...
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bioRxiv - Immunology 2021Quote: ... A prime dose of 20µg of antigen in TiterMax Gold (Sigma-Aldrich) was administered intramuscularly in the hind leg(s) ...
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bioRxiv - Immunology 2021Quote: ... Antigen retrieval protocol were achieved using Citrate buffer pH 6 (Sigma-Aldrich). Sections were saturated during 30 minutes in PBS 10% Human Serum 0,3% Saponin and incubated at 4°C with primary antibody overnight in PBS 10% Human Serum 0,3% Saponin ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Proteins and antigens were wet-transferred in TGS 1X (Sigma T7777-1L) with methanol 20% to a nitrocellulose membrane (Amersham 10600004 ...
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bioRxiv - Immunology 2020Quote: ... antigens were buffer-exchanged and concentrated using Amicon centrifugation filters (EMD Millipore) into phosphate buffer at pH 5.8 and pH 7.2 prior to use ...
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bioRxiv - Pathology 2022Quote: ... we used antigen heat retrieval using citrate buffer pH 6.0 (Sigma, C9999), with the exception of EDTA pH 9.0 antigen heat retrieval for serotransferrin and elastin ...
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bioRxiv - Neuroscience 2022Quote: ... Antigen retrieval was performed for 10 minutes using Pepsin reagent (R2283, Sigma). Samples were washed twice with PBS containing 0.025% triton-x for 5 minutes and then blocked with protein block (ab64226 ...