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Citations for Millipore Sigma :
101 - 150 of 10000+ citations for Mitochondrial dimethyladenosine transferase 1 TFB1M Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... mCherry-CAMSAP2 was eluted in 50 μl TEV cleavage buffer containing 0.5 μg of glutathione S-transferase-6x-histidine Tobacco etch virus protease site (Sigma-Aldrich) for 2 hr at 4 °C.
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bioRxiv - Molecular Biology 2023Quote: ... The ApopTag Peroxidase In Situ Apoptosis Detection Kit was used for Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (Sigma Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... cell or tissue pellets were resuspended in Cell/Mitochondrial Lysis Buffer (as recommended by Millipore) with protease inhibitors (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: The capacity to polarize mitochondrial membrane was determined fluorometrically employing safranin O dye (Sigma S2255) in permeabilized cells ...
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bioRxiv - Molecular Biology 2020Quote: Complex III activity was assayed using a mitochondrial complex III activity assay kit (Sigma Aldrich) and complex IV activity was determined using a complex IV human enzyme activity microplate assay kit (Abcam) ...
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bioRxiv - Cell Biology 2022Quote: ... The mitochondrial activity of the cells was determined after the reduction of MTT (Sigma-Aldrich) to produce formazan crystals [95] ...
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bioRxiv - Cell Biology 2020Quote: ... Cell death was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (ApopTag Plus Peroxidase In Situ Apoptosis Detection Kit, Millipore-Sigma). Cell proliferation was evaluated by Ki-67 immunostaining (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2021Quote: ... were obtained using terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay (82) (based on in Situ Cell Death Detection Kit, 11684795910, red fluorescence; Sigma-Aldrich) on sections derived from rd1 and C3H retinal explant cultures ...
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bioRxiv - Cancer Biology 2020Quote: The sections from two biological replicate experiments were stained using a Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay (Millipore S7165), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The ApopTag Peroxidase In Situ Apoptosis Detection Kit was used for Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (Sigma Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... OC antibody of 1:25,000 and A11 antibody of 1:500 (Millipore and Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... and brain the mitochondrial isolation buffer was additionally supplemented with 0.2% bovine serum albumin (Sigma-Aldrich). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: Mitochondrial samples from proteinase-K treatment were thawed and resuspended in RIPA buffer (Millipore #20-188) containing protease inhibitors (Sigma #P8340) ...
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bioRxiv - Neuroscience 2021Quote: Relative mitochondrial aconitase activity was quantified using the colorimetric Aconitase Activity Assay Kit from Sigma (#MAK051), following manufacturer’s instructions as previously described (Martelli et al. ...
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bioRxiv - Microbiology 2019Quote: ... and the mitochondrial GFP reporter strain AfS35 pJW103 (49) were maintained on malt extract (Sigma-Aldrich) agar plates supplemented with 2% (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... The standard mitochondrial stress test was performed with injection of FCCP (2 μM; Sigma-Aldrich, C2920), oligomycin (1 μM ...
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bioRxiv - Immunology 2023Quote: ... Concentrations of the Mitochondrial stress assay inhibitors are as follows: 1µM oligomycin (Sigma-Aldrich, 75351-5MG), 2µM FCCP (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2 μM rotenone and antimycin A (mitochondrial complex I and III inhibitors, respectively; Sigma-Aldrich). Cells were counted at the end of the experiments to normalize the OCR ...
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bioRxiv - Cell Biology 2024Quote: Mitochondrial membrane potential was evaluated using the potentiometric dye tetramethyl rhodamine methyl ester (TMRM; Sigma Aldrich) at a final concentration of 10 nm ...
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bioRxiv - Bioengineering 2020Quote: ... PCM-1 antibody (1:400, Sigma-Aldrich) and concentration matched rabbit IgG isotype control (Novus Biologicals ...
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bioRxiv - Physiology 2021Quote: ... terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was performed using an ApopTag® In Situ Peroxidase Detection Kit (S7100, Merck Millipore, USA) according to the manufacturer’s recommendations and as previously described (36) ...
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bioRxiv - Biochemistry 2021Quote: ... Carboxyl-terminal histidine or N-terminal glutathione-S-transferase fusion tags were added using the respective plasmids pET21b (Novagen, Madison, Wisconsin, USA) and pGEX-4T3 (Amersham Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was performed using the ApopTag® Peroxidase in situ Apoptosis Detection Kit (EMD Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... were split to 2.0 x 106 cells/mL in a 10 mL volume 24h prior to transfection and treated with 100 μM of a fucosyl transferase inhibitor, 2F-Peracetyl-Fucose (Rillahan et al., 2012) (EMD Millipore, 344827). On the day of transfection ...
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bioRxiv - Cell Biology 2023Quote: ... was cloned into a pLIC vectors with a TEV-cleavable N-terminal Glutathione S-transferase tag and transformed into Rosetta™ 2 (pLysS) competent cells (Sigma-Aldrich). GST-Cse4END was purified as described above ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then incubated with anti-CDK2 monoclonal antibody (1:100) and anti-SCP1 polyclonal antibody (NB300-229, Novus, 1:100) or anti-RAD51 polyclonal antibody (pc130, Millipore, 1:100) and anti-SYCP-3 monoclonal antibody (sc-74569 ...
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bioRxiv - Biochemistry 2021Quote: Mitochondrial CS (#SLCC7842) and MDH (#SLCD5169) isolated from porcine hearts were purchased from Millipore Sigma (München, Germany). MST dilution Buffer was composed of 0.1 M potassium phosphate buffer containing 0.05% Tween 20 with pH 8 unless otherwise stated.
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... to 20 μg of mitochondrial protein and run with Tris-MOPS running buffer (#PCG3003; Sigma-Aldrich/Merck) on a 4-20% precast Trupage gel (#PCG2012-10EA ...
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bioRxiv - Immunology 2023Quote: ... whereas carbonilcyanide p-triflouromethoxy-phenylhydrazone (FCCP, an ionophore that permeabilize the inner mitochondrial membrane to H+, Sigma) was used at a concentration of 10 μM ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (PSD95 1:500, Antibodies Inc., Davis CA and MAP2 1:1000; Millipore) were diluted in 1x PBS with 1% BSA and 0.2% Tween ...
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bioRxiv - Physiology 2019Quote: ... Pax7 primary antibody (1:100, DSHB) and laminin primary antibody (1:100, Sigma-Aldrich) were diluted in 1% TSA blocking buffer and applied overnight ...
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bioRxiv - Neuroscience 2020Quote: ... Antibodies: Trim28 (Millipore, 1:500), IAP-gag (a kind gift from Bryan Cullen and described in (Dewannieux et al. ...
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bioRxiv - Neuroscience 2020Quote: ... GluA2 antibody (1:2,000, Millipore), pGluA1-Ser845 antibody (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... β-actin antibody (1:20,000, Sigma), GAPDH antibody (1:20,000 ...
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bioRxiv - Neuroscience 2020Quote: ... Abcam),Tubulin antibody (1:1,000, Millipore) at 4℃ for 12h ...
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bioRxiv - Neuroscience 2022Quote: ... Antibody SMI32 (Millipore; 1:10,000) recognizes non-phosphorylated neurofilament protein in axons ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies included rhodopsin (1:1 000, Sigma), sw-opsin (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... the cytosolic and mitochondrial fractions were 10-fold concentrated using Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Measurement of IDH activity on the enriched mitochondrial fractions was conducted using an IDH activity assay (Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated with the following mitochondrial toxins for 16 h: 1.25 ng/mL oligomycin (Sigma-Aldrich # 75351), 50 µg/mL doxycycline (Clontech # 631311) ...
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bioRxiv - Physiology 2020Quote: ... Intracellular and mitochondrial superoxide generation was measured using dihydroethidium (DHE, 5 μM, 20 minutes) (Sigma-Aldrich, Dorset, UK) and MitoSOX Red mitochondrial superoxide indicator (5 μM ...
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bioRxiv - Cell Biology 2020Quote: ... Mitochondrial pellets were resuspended in 100 μl RIPA buffer supplemented with EDTA-free protease inhibitor cocktail (EMD Millipore) under constant agitation for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 97062]) and then overlaid with 175μL of mitochondrial assay solution supplemented with 10mM sodium pyruvate (Sigma-Aldrich, P5280), 1mM malate (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed twice with mitochondrial assay solution (220mM mannitol [Sigma-Aldrich, M4125], 70mM sucrose [Sigma-Aldrich, S7903] ...
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bioRxiv - Microbiology 2023Quote: ... opening of the mitochondrial permeabilization transition pore (mPTP) was visualized by tetramethylrhodamine methyl ester perchlorate (TMRM) staining (Sigma). Cells were washed once with 1 x HBBS (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... mitoProfile total OXPHOS Human WB antibody cocktail (1:1000, ab110411), SDHA monoclonal antibody (1:10000, 459200), anti-TOMM20 (1:1000, HPA011562) and actin antibody (1:1200, Sigma A2066). Four 5 min washes with PBS-T (0.005% tween ...
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bioRxiv - Developmental Biology 2021Quote: ... The antibodies: Chicken anti-GFP antibody (ab16901, 1:500; Millipore), Mouse anti-Satb2 (ab51502 ...
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bioRxiv - Systems Biology 2020Quote: ... Antibodies used included: Rac1 antibody (Millipore, clone 238A, 1:500), anti-RhoA antibody (Santa Cruz Biotechnology 26C4 ...
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bioRxiv - Biochemistry 2019Quote: ... 1 μL of antibody (α-Flag-M2 antibody, Sigma-Aldrich / α-HA ...
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies (1:1000) and DAPI (D9542-1, Sigma, 1:10,000) were incubated for 2hrs at room temperature ...