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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Mayaro Virus E2 Envelope Protein Mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... E2 was added to differentiation media once daily for nine days (10−8 M, Sigma Aldrich). For luteal conditions (abbreviated as EP) ...
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bioRxiv - Biochemistry 2021Quote: SUMO-peptide fusions were cloned into a pDW363 vector that appends a BAP sequence and 6x-His tag to the N-terminus of the protein and transformed into Rosetta2(DE3) cells (Novagen). Rosetta2(DE3 ...
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bioRxiv - Microbiology 2020Quote: ... The analyzed proteins contained an SHH-tag (Sarkari et al., 2014) and were detected using anti-HA (Sigma-Aldrich, USA) antibodies and anti-mouse IgG-HRP (Promega ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was resuspended in digest buffer (0.1 M triethylammonium bicarbonate, pH 8.5, Sigma-Aldrich, tandem mass tag (TMT) labeling using a 6-plex TMT kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... was cloned into the protein expression vector pET28a (+) containing an IPTG-inducible promoter and a C-terminal 6x-His tag (Novagen). Expression of CosR-His was then induced in E ...
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bioRxiv - Developmental Biology 2020Quote: Coding regions for all protein fragments used for EMSA were cloned in-frame with an N-terminal 6xHis-tag in the pET14b vector (Novagen) and expressed in BL21 cells ...
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bioRxiv - Cell Biology 2024Quote: Antibodies that were used in this study to detect viral and cellular proteins were the monoclonal antibody (MAb) recognizing the FLAG-tag epitope anti-Flag (clone M2, Sigma), the MAb against HCMV pp28 ...
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bioRxiv - Plant Biology 2024Quote: ... Phosphorylation of NopTC93S proteins was analyzed by 50μM Zn2+-Phos-tag SDS-PAGE and detected with anti–FLAG (Sigma, F1804) antibodies by immunoblot analysis.
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bioRxiv - Cancer Biology 2024Quote: ... and the RDF plasmid encoding the RD114 envelope) using the GeneJuice transfection reagent (Merck Millipore). Viral supernatant was collected 48 and 72 hours after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA sequence encoding 1053-1264 aa mouse was amplified and cloned upstream of sequences encoding a polyhistidine stretch (His) tag in pET23b (Novagen). The mGli2-His recombinant protein was expressed in BL21 (DE3 ...
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bioRxiv - Microbiology 2020Quote: ... All purified proteins were analyzed by SDS-PAGE followed by Coomassie blue staining as well as by Western blot using a mouse monoclonal antibody against the 6xHis-Tag (Novagen). Protein aliquots were stored at −80⁰C in 50% glycerol (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... Bound scFv were detected using murine mAb 9E10 which recognizes the C-terminal c-myc tag (1:50 diluted in 2% MPBST) and a goat anti-mouse serum conjugated with horseradish peroxidase (HRP) (A0168, Sigma) (1:42000 dilution in 2% MPBST) ...
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bioRxiv - Bioengineering 2019Quote: For Flag-tag detection membranes were incubated for two hours with the monoclonal anti-Flag M2 antibody from mouse (F3165, Sigma) in a 1:5000 dilution (in TBS-T ...
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bioRxiv - Physiology 2020Quote: ... The presence of LPL in the conditioned media was confirmed through Western blotting using a mouse antibody against the FLAG tag (1:5000; Sigma). LPL activity was assessed through an LPL activity assay (see below).
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bioRxiv - Immunology 2019Quote: ... resuspended in RPMI-10% FBS containing 40ng/mL CTLA4-Ig (CD152/Fc chimera, non-cytolytic, from mouse, Sigma C4358), plated in 24-well culture plates at a density of 2 x 106 cells/well and cultured for an additional 24 hours (BMDCCTLA4-Ig) ...
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bioRxiv - Microbiology 2024Quote: ... The secondary antibody used was goat anti-mouse IgG (Fc specific) conjugated with alkaline phosphatase from Sigma (CAT#A1418) and used at 1:3000 dilution.
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bioRxiv - Cell Biology 2019Quote: ... FLAG tag from Sigma Aldrich (F3165); SHIP2 and phosphorylated SHIP2-Y1135 from Cell Signaling Technologies (#2839 and 5445 ...
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bioRxiv - Cell Biology 2020Quote: ... HA-tag (Sigma-Aldrich# H-9658), FLAG (Sigma-Aldrich#) ...
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bioRxiv - Developmental Biology 2020Quote: The Apop-Tag Fluorescein Kit (Millipore) was used for TUNEL staining according to manufacturer’s instruction with minor modifications ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG epitope tag (F7425; Sigma-Aldrich). Smc2 (ab10399 ...
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bioRxiv - Cell Biology 2019Quote: ... Myc-Tag (JBW301, Millipore, 05-724), GAPDH (Cell signaling ...
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bioRxiv - Microbiology 2019Quote: ... pTAC-MAT-Tag-2 (Sigma-Aldrich) was PCR-amplified with primers pTAC-Gbs-for/rev (Table S9) ...
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bioRxiv - Cell Biology 2021Quote: ... HA-tag (HA.C5, Millipore 05-904) or FLAG-tag (M2 ...
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bioRxiv - Cell Biology 2021Quote: ... or FLAG-tag (M2, Sigma, F3165) at 4°C ...
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bioRxiv - Physiology 2021Quote: ... the FLAG tag (F1804, Sigma-Aldrich), and Fam64a ...
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bioRxiv - Plant Biology 2021Quote: ... HA- or Myc-tags (Sigma-Aldrich) followed by staining with secondary antibodies coupled to alkaline phosphatase (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-HA tag (Clone 6E2, Millipore).
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bioRxiv - Microbiology 2020Quote: ... Flag-tag (1:5,000, M2, Sigma), V5-tag (1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... Flag-tag (F7425, Sigma, 1:1,000), Myc-tag (Cat ...
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bioRxiv - Biochemistry 2019Quote: ... anti-FlagM2-tag (F3165; Sigma-Aldrich), anti-GFP-tag (Living Colors 632592 ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-Myc Tag (Millipore, 05-724), anti-mouse-HRP (GE healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... FLAG-tag (clone M2, SIGMA-ALDRICH), FLAG-tag (Thermo ...
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bioRxiv - Molecular Biology 2022Quote: ... myc-tag and GAPDH (Sigma-Aldrich); S6K1 (GenScript) ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG eptitope tag (M2, Sigma, #F3165), GFP (JL-8 ...
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bioRxiv - Molecular Biology 2022Quote: ... Flag-tag (Sigma, F3165, 1:3000). For immunofluorescence staining ...
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bioRxiv - Microbiology 2023Quote: ... HA tag (HRP coupled, Sigma; H6533), β-actin (HRP coupled ...
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bioRxiv - Neuroscience 2023Quote: ... His•Tag® (Millipore, 70796-3), anti-APP C-Terminal Fragment (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... FLAG tag (Sigma-Aldrich, cat F3165), β-actin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-FLAG epitope tag (L00018; Sigma), Rabbit polyclonal anti-SARS-CoV-2 nucleoprotein N protein (40068-RP01 ...
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bioRxiv - Immunology 2021Quote: ... with 10% FCS (Sigma-Aldrich) and 100 U/ml penicillin plus 100 mg/ml streptomycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: ... Fc IgG (AG714, Merck Millipore) at 2 μg/ml in 150 μl of PBS for one hour ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% FCS (Sigma), 2mM L-glutamine (Thermofisher) ...
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bioRxiv - Genomics 2020Quote: ... 15% FCS (Sigma ES-009), 100 units/ml penicillin ...
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bioRxiv - Immunology 2021Quote: ... and 1% FCS (Sigma-Aldrich) three times for 15 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% FCS (Sigma), 2 mM L-glutamine (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 8% FCS (Sigma), penicillin/streptomycin (Roche ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% FCS (Sigma), 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2022Quote: ... containing 10% FCS (Sigma-Aldrich), 100U/ml penicillin and 100μg/ml puromycin (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... with 10% FCS (Sigma-Aldrich), 750 μl milk EVs or EV-depleted controls in a total volume of 1 ml and cultured for the indicated amount of time.
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bioRxiv - Immunology 2020Quote: ... with 10% FCS (Sigma-Aldrich), 750 μl milk EVs or EV-depleted controls in a total volume of 1 ml and cultured for 6 days ...