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Citations for Millipore Sigma :
51 - 100 of 10000+ citations for Mayaro Virus E2 Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... + 10 nM estradiol (E2; Sigma-Aldrich E2758) + 100 nM progesterone (P4 ...
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bioRxiv - Genomics 2023Quote: ... 2.5 µM prostaglandin E2 (Sigma-Aldrich, P0409), 2 µM Y-27632 (Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... and 10nM E2 (Sigma, Cat. #E1024-1G). Conditioned media was produced in HEK293 cells and obtained from the Center for Digestive Diseases and Organoid Core Facility at Baylor College of Medicine ...
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bioRxiv - Microbiology 2022Quote: ... Plates with Towne virus were stained with mouse anti-human CMV IE1 (MAB810, Millipore) then goat anti-mouse IgG-AF488 (Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 ug/ml of Fc are mixed with 2.5 ug/ ml of Anti-human IgG (Fc specific) FITC (Sigma, F9512) and Anti-human IgG (Fc specific ...
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bioRxiv - Biochemistry 2022Quote: ... horseradish peroxidase (HRP)-conjugated anti-human IgG Fc specific antibody (Sigma, USA) was added at the dilution of 1:2,000 and incubated at 37 °C for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... with goat anti-human Fc-specific IgG (Sigma-Aldrich – Saint Louis, MO) immobilized with a target capture level of ~70 RU ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). To enrich for MSCs ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). After blocking ...
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bioRxiv - Immunology 2022Quote: ... blocking of Fc receptors with human Ig (Sigma-Aldrich, St. Louis, MO); surface staining with mouse anti-human CD3-APC-H7 ...
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bioRxiv - Immunology 2023Quote: ... Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). For analysis of neutrophil activation ...
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bioRxiv - Bioengineering 2024Quote: ... or horseradish peroxidase-conjugated goat anti-human IgG Fc antibody (Sigma, A0170) diluted 1:20,000 in the blocking buffer for an hour at 37°C ...
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bioRxiv - Plant Biology 2019Quote: ... The recombinant proteins with either FLAG-tag or His-tag were purified by corresponding immune affinity agarose beads (Sigma).
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bioRxiv - Genomics 2021Quote: ... and either 1 uM Prostaglandin E2 (PGE2) (Sigma) or 3 ng/ml TGFB (R&D Systems) ...
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bioRxiv - Neuroscience 2021Quote: ... containing 50 μg of E2-benzoate (Sigma-Aldrich). Four hours before the tests ...
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bioRxiv - Cancer Biology 2021Quote: 17β-estradiol (E2, #E8875) was obtained from Sigma, and Fulvestrant (#1047) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 nM β-Estradiol (E2) (Sigma-Aldrich, USA) and 1 µM medroxyprogesterone 17-acetate (MPA ...
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bioRxiv - Bioengineering 2022Quote: ... 1 nM 17-β estradiol (E2, Sigma Aldrich) and 1× Pen-Strep solution (Sigma Aldrich) ...
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bioRxiv - Systems Biology 2024Quote: ... E2 (estradiol, final conc. 10 nM; Sigma E1024), 8-Br cAMP (8-Bromoadenosine 3′,5′-cyclic monophosphate ...
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bioRxiv - Immunology 2021Quote: ... Envelope supernatants were immobilized onto ConA (Sigma, catalog no C2272 ...
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bioRxiv - Immunology 2020Quote: ... except anti-flaviviral envelope 4G2 (Merck Millipore), β-Tubulin ...
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bioRxiv - Developmental Biology 2023Quote: ... strep tag was removed from the eluted proteins by thrombin (Sigma) digestion overnight at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... protein tags were removed by tobaccoetchvirus (TEV) protease (#T4455, Sigma-Aldrich) (1:25 w/w ...
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bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS + 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with CD15-APCCy7 (1:100 ...
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bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS containing 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with the following antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... ephrinB1-Fc (R&D, #473-EB) or ephrinB2-Fc (R&D, #496-EB) were pre-clustered using goat anti-human Fc IgG (Sigma, #I2136) in 4:1 ratio for 30min.
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bioRxiv - Microbiology 2020Quote: ... The flow-through from the His-tag cleavage containing the tag free protein was concentrated to 2 mL using Amicon Ultra-4 30k MWCO (Millipore). The concentrated protein was applied to a HiLoad 16/600 S75pg column (GE Life Sciences) ...
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bioRxiv - Molecular Biology 2019Quote: ... was fused with an N-terminal His(6)-tag followed by maltose binding protein (MBP) tag and TEV cleavage site in pET21 vector (Novagen).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in which the plate was coated with anti–human Fc (Sigma, Catalog # FAB3700259), and after overnight incubation at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged and non-Fc-tagged proteins were concentrated using Amicon Ultra 10 kDa MWCO centrifugal filter units (Millipore), dialyzed against PBS ...
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bioRxiv - Systems Biology 2021Quote: ... virus protein-bound host proteins were enriched by adding 50 µl anti-HA-agarose slurry (Sigma-Aldrich, A2095) with constant agitation for 3 hours at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... The binding of VHH-Fcs/ACE2-Fc to S1 was detected using 100 µL 1 µg/mL HRP-conjugated goat anti-human IgG (Sigma, Cat#A0170). Finally ...
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bioRxiv - Bioengineering 2021Quote: ... plates were washed 10 times with PBST and the ACE2-Fc binding was detected using 1 µg/mL goat anti-human IgG (Fc specific) HRP conjugate antibody (Sigma, Cat#A0170) in 100 µL PBSCT ...
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bioRxiv - Neuroscience 2021Quote: ... 5ug cyclodextrin-encapsulated E2 in 5uL saline (Sigma-Aldrich), or 5uL free cyclodextrin vehicle (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... 10 nM 17b-estradiol-acetate (estrogen E2, Sigma E7879) for 5 days as originally described (71) ...
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bioRxiv - Cancer Biology 2022Quote: ... Hormones used were 17β-estradiol (E2, Sigma Aldrich, E2758), (Z)-4-Hydroxytamoxifen (4OHT ...
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bioRxiv - Cancer Biology 2024Quote: ... 17-β-estradiol (E2) (Cat# E-060, Sigma Aldrich) was used in all assays at a physiologic concentration of 1 nM/mL ...
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bioRxiv - Genetics 2021Quote: ... Each ORF cloned into the pLVX-EF1a-IRES-PURO/eGFP lentiviral expression vector was added to lentiviral packaging and envelope vectors (Sigma-MISSION-gag-pol and the envelope Sigma-MISSION-VSV-G (Cat ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tag of the eluted protein was cleaved by thrombin (Sigma), incubated at 4 °C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... MaxiSorp plates (Thermo) were coated with 100µl of goat anti-human Fc antibody (Sigma) diluted 1:500 in PBS overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed before detection and goat anti-human Fc-HRP conjugated antibody (Sigma) (1:5000 dilution in PBS ...
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bioRxiv - Neuroscience 2020Quote: MaxiSorp plates were coated with 100 µl of goat anti-human Fc antibody (Sigma) diluted 1:500 in PBS or 3 mg/ml of an anti-VNAR antibody (courtesy of Martin Flajnik ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg/mL goat anti-human IgG1 Fc F(ab’)2 (Sigma-Aldrich, I3391) was coated in 96-well NUNC Immunoplate (Thermo Fisher ...
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bioRxiv - Physiology 2023Quote: Rm-EphrinA4/Fc chimera (R&D) was clustered with anti-human Cy3 antibody (Sigma) diluted 1 ...
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bioRxiv - Biochemistry 2023Quote: ... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
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bioRxiv - Biophysics 2021Quote: Fibrinogen (human plasma purified protein, Sigma-Aldrich)
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bioRxiv - Cancer Biology 2019Quote: ... 17β-estradiol (E2) were purchased from Sigma Aldrich (MO, USA). Routine laboratory buffers ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 nM 17-beta estradiol (E2) (all from Sigma-Aldrich) and 2.5 mIU/ml FSH (Merck ...
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bioRxiv - Molecular Biology 2023Quote: ... 17β-estradiol (E2) and lasofoxifene (Las) were purchased from Sigma, 4-hydroxytamoxifen (4-OHT) ...
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bioRxiv - Developmental Biology 2023Quote: ... 17D-estradiol (E2) (E8875, Sigma-Aldrich, St. Louis, MO, USA) and progesterone (P4 ...