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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for Mayaro Virus E2 Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Recombinant lyophilized proteins were resuspended using 0.1% (w/v) human serum albumin (HSA) (A9731, Sigma-Aldrich) in sterile water ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies to human proteins used in this study included: Actin (1:20,000 dilution (MAB1501, Millipore, RRID:AB_2223041)) ...
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bioRxiv - Neuroscience 2021Quote: ... Animals were subsequently administered 5 μg 17-β-estradiol benzoate in 50uL sesame oil (E2; Sigma-Aldrich, St. Louis, MO) on Day 2 ...
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bioRxiv - Neuroscience 2021Quote: ... OVX females received 5 μg 17-β-estradiol benzoate in 5uL sesame oil (E2, SC; Sigma Aldrich, St. Louis, MO) followed by 10μg E2 in 10μL sesame oil 24 h later ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 weeks and 8 weeks old C57BL/6J mice were administered with E2 (Cat No. E-2758, Sigma, MO, USA), which was dissolved in ethanol and diluted in corn oil (Cat No ...
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bioRxiv - Immunology 2022Quote: ... A second cohort of SARS-CoV-2-infected male hamsters were intravenously co-injected with 18F-FES and 0.3 mg/kg of E2 (3% DMSO; Sigma-Aldrich) on day 7 post-infection (n = 4) ...
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bioRxiv - Microbiology 2022Quote: ... the isolates were cultured overnight at 37 °C in alkaline nutrient agar (20 g casein meat peptone E2 from Organotechnie; 5 g sodium chloride from Sigma; 15 g Bacto agar from Difco ...
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bioRxiv - Microbiology 2022Quote: ... larvae were kept at 28.5°C in E2 medium with 100 μM diphenyleneiodonium chloride (DPI, Sigma-Aldrich cat no. D2926) 84 and 10 μM cyclosporin A (CsA ...
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bioRxiv - Microbiology 2022Quote: ... strain CNRVC19247 was cultured in alkaline nutrient agar (casein meat peptone E2 from Organotechnie, 20 g; sodium chloride from Sigma, 5 g ...
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bioRxiv - Neuroscience 2022Quote: ... The sex steroid-containing feed was prepared by mixing 5 mg dry feed with 10-100 ng of 11-KT or E2 (Sigma) using 96% ethanol as the vehicle and then evaporated at 40 °C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Both embedded or suspension culture organoids were grown for 4 days after passaging and then treated with either vehicle as a control (100% ethanol) or 10□nM estradiol-17β (E2; Cat. No. E1024; Sigma) for 2 days ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were treated with either vehicle control or 10 nM E2 and 1 μM medroxyprogesterone acetate (Cat. No. PHR1589; Sigma) for 6□days with media changed every 2□days ...
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bioRxiv - Cancer Biology 2023Quote: ... T47D and MCF-7 cells were incubated in phenol red-free charcoal-stripped medium with E2 (10 nM; Sigma-Aldrich) for 5 min and 15 min/37°C ...
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bioRxiv - Genomics 2023Quote: ... Subsets of male and female mice on HFD were additionally injected intraperitoneally with the estrogenic ligands 17β-estradiol (E2, 0.5mg/kg body weight, Sigma-Aldrich), 4,4’,4’’-(4-Propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol (PPT ...
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bioRxiv - Evolutionary Biology 2024Quote: Mouse and macaque endometrial epithelial organoids were treated for two consecutive days with 17-ß estradiol (E2) (Sigma Aldrich, E8875) at 10 nM or for two days with 17-ß estradiol (E2 ...
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bioRxiv - Plant Biology 2019Quote: ... and axr3-3 were expressed as 6X His-tag (N-terminal) fusion proteins in Escherichia coli strain Rosetta™ DE3 competent cells (Supplementary Table 4; Novagen, product code: 70954). These proteins were expressed in minimal media with 13C D-glucose and 15N ammonium chloride ...
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bioRxiv - Biochemistry 2019Quote: ... rabbit monoclonal DYKDDDDK tag (Flag tag, 1:1000, CST, #14793) and/or mouse monoclonal α-tubulin (1:10,000, Millipore, 05-829). Following three washes with TBS-T ...
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bioRxiv - Biochemistry 2022Quote: ... residual bound Coa and Efb were detected with anti-tag HRP-conjugated antibodies diluted 1:10000 in 2%BSA-PBST: α-HIS-tag antibody (A7058, Sigma) for EfbN ...
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bioRxiv - Molecular Biology 2020Quote: ... cleaved His-tag and His-tag-fused TEV protease were removed using a HIS-Select® Nickel Affinity Gel (Sigma-Aldrich) column ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µL from the supernatant and cell lysates were applied on a nylon membrane and two different antibodies (an HRP-conjugated anti-His tag from Qiagen and an HRP-conjugated anti-strep tag from Sigma-Aldrich) were used on separate blots to detect PR3 ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding to N protein was detected using conventional two-step ELISA with an HRPO-conjugated anti-mouse or anti-rat Fc antibody (Sigma Aldrich cat # A9309). All assays included a standard curve of mouse neutralising S1 antibody and mouse anti-N antibody (Sino Biological) ...
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bioRxiv - Bioengineering 2023Quote: ... Either 15 μg of nanobody Fc plasmid or 1.5 μg of nanobody Fc plasmid and 13.5 μg of ssDNA (Sigma, D7656) were combined with 45 μg PEI (Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... 2 μg of VSV envelope vector pMD2.G and 15 nmol linear 25 kDa polyethylenimine (Millipore Sigma, Burlington, MA). Media was replaced 24 hours later and ViralBoost Reagent (Alstem ...
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Post-stroke rapamycin treatment improves post-recanalization cerebral blood flow and outcome in ratsbioRxiv - Neuroscience 2023Quote: Animals were randomized with the sealed envelope method to receive either rapamycin (Sigma Aldrich, 250 μg/kg, n=9) or vehicle (<5% ethanol in saline ...
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bioRxiv - Molecular Biology 2021Quote: ... with subsequent immunoblotting against either the FLAGepitope tag or TWIN-Strep epitope tag (Anti-FLAG M2, Sigma-Aldrich; THETM NWSHPQFEK antibody, GenScript).
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bioRxiv - Molecular Biology 2022Quote: Antibodies that recognize the following proteins and epitope tags were used: glucose-6-phosphate dehydrogenase (G6PDH; Sigma-Aldrich A-9521, 1:20,000 or 1:50,000), H2B (Active motif 39237 ...
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bioRxiv - Molecular Biology 2021Quote: ... The virus was propagated on VeroE6-TMPRSS2 cells using DMEM (Sigma) supplemented with 2% (v/v ...
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bioRxiv - Bioengineering 2021Quote: ... virus was harvested and purified using an iodixanol gradient (Optiprep, Sigma). For our 7-variant pool ...
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bioRxiv - Neuroscience 2021Quote: ... Virus or Tracer was loaded into mineral oil (Sigma-Aldrich, M3516)-filled glass pipette (WPI ...
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bioRxiv - Microbiology 2021Quote: ... virus was concentrated using 100,000 MW cut-off Centricon concentrators (Millipore).
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bioRxiv - Immunology 2019Quote: ... thawed virus was lysed in Triton-X (Sigma; 9002-93-1) and used for p24 measurement at the UCLA CFAR Virology core ...
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bioRxiv - Neuroscience 2021Quote: ... This concentrated virus solution was filtered (0.22 μm Millex, Merck Millipore) and stored at 4 °C until further use ...
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bioRxiv - Cell Biology 2019Quote: ... we added concentrated virus and 5 μg/ml polybrene (Sigma-Aldrich) to MCF10A cells in growth media ...
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bioRxiv - Immunology 2022Quote: ... virus supernatant was filtered through a 0.44 um PVDF membrane (Millipore), incubated with Lipofectamine 3000 and PLUS reagent as above ...
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bioRxiv - Microbiology 2023Quote: ... the virus containing medium plus 8 µg/ml polybrene (Sigma #H9268) and the infected 293T cells were co-cultured with iSLK cells ...
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bioRxiv - Cell Biology 2023Quote: ... the virus was mixed with fully supplemented DMEM (Sigma-Aldrich, D6429) and 8 μg/ml Polybrene (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... after 3 DIV the medium of some cultures was replaced for 2 h by fresh medium devoid of B-27 and GlutaMAX-I supplement and then cells were incubated for 2 h with 10-10 M E2 (Sigma-Aldrich) or vehicle ...
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bioRxiv - Developmental Biology 2022Quote: ... was dissolved in distilled water fresh for each use and diluted in E2 supplemented with 0.04% MS-222 (tricaine, Sigma Aldrich E10521) to a working concentration of 10 ...
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bioRxiv - Biochemistry 2023Quote: Two complementary single strand oligonucleotides (5’-GTAATCTGGGCCACCTGCCTGGGAGGA-3’ and 5’-TCCTCCCAGGCAGGTGGCCCAGATTAC-3’) corresponding E2 box44 were Two complementary single strand oligonucleotides (5’-purchased from Sigma-Aldrich. An equal molar ratio of single strand oligonucleotides was mixed and incubated at 95°C for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... wells were washed three times using H2O 0.05% Tween20 and binding of NMDAR antibodies to the immobilized N1-N2B-Fc was detected by using goat-anti-hIgG(Fc)-HRP (1:70000, A0170, Sigma). Binding was visualized by using TMB substrate ...
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bioRxiv - Cell Biology 2020Quote: ... and S-tag (EMD Millipore, MAC112, WB: 1:5000). Rat monoclonal antibody ...
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bioRxiv - Physiology 2020Quote: ... Plasma TAG was measured with a colorimetric assay (Sigma).
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Neuroscience 2019Quote: ... anti-6xHis-tag mouse monoclonal antibody # H1029 (Sigma-Aldrich), anti-tubulin # T8660 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... HRP-conjugated mouse anti-FLAG tag Ab (Sigma-Aldrich) was used to detect Fab/VH binding ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies to Flag-tag (F3165) were purchased from Sigma. Antibodies to pT308-Akt (ab38449 ...
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bioRxiv - Plant Biology 2021Quote: ... or western blot using HA Tag Antibodies (Sigma-Aldrich).
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tag (M2) and α-Tubulin (T5168) from Sigma, RFP (600-401-379 ...
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal Flag-tag antibody (Sigma, F3165, 1:1000), rabbit monoclonal HA-tag antibody (CST ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-Flag tag (Sigma cat# F1804, 1:1000), rabbit anti-human actin (Sigma cat#A2066 ...