Labshake search
Citations for Millipore Sigma :
1 - 50 of 1510 citations for MERS Coronavirus Spike Glycoprotein S1 Camel Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein (S1) (#E5S3V) and GAPDH (G9545, Sigma-Aldrich) were incubated overnight at 4 °C in TBS-T ...
-
bioRxiv - Immunology 2021Quote: ... HEK293T cells expressing coronavirus spikes were generated by transient transfection using pcDNA3 or pCMV3-based expression plasmids and GeneJuice (EMD Millipore) 48 hours prior to use ...
-
bioRxiv - Microbiology 2022Quote: ... anti-coronavirus antibody (MAB9013, Merck Millipore), SARS-CoV-2 nucleocapsid antibody (HL344 ...
-
bioRxiv - Biochemistry 2023Quote: Purified SARS-CoV-2 spike glycoproteins were exchanged to water using Microcon Ultracel PL-10 centrifugal filter (Millipore Sigma). Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl ...
-
bioRxiv - Genomics 2022Quote: ... These 16 spike-in oligomers were purchased in two separate batches (Table S1) from Sigma Aldrich and resuspended in 10 mM Tris (pH 8 ...
-
bioRxiv - Microbiology 2022Quote: ADCP assays were performed using ExpiCHO-S cells transiently transfected with SARS-CoV-2 spike glycoproteins (Wuhan D614, BA.1, or BA.2) and labelled with PKH67 (Sigma Aldrich) as targets ...
-
bioRxiv - Bioengineering 2021Quote: ... The binding of VHH-Fcs/ACE2-Fc to S1 was detected using 100 µL 1 µg/mL HRP-conjugated goat anti-human IgG (Sigma, Cat#A0170). Finally ...
-
bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
-
bioRxiv - Microbiology 2020Quote: HEK293 cells were transfected with plasmids encoding different ACE2 orthologs (Table S1) by polyetherimide (PEI) (Sigma, St Louis, MO, USA). After 40 hrs incubation ...
-
bioRxiv - Microbiology 2021Quote: ... the Fc tag of all APN orthologs was removed using thrombin (Millipore Sigma) in a reaction mixture containing ...
-
bioRxiv - Genomics 2019Quote: ... 60-mer ds-oligos were synthesized (Sigma-Aldrich) for both alleles of either rs1152591 or rs1152595 ...
-
bioRxiv - Plant Biology 2022Quote: ... Acetosyringone (200μM final conc., Sigma/Mer-ck #D134406), and Synperonic PE/F68 (0.1% final conc. ...
-
bioRxiv - Cell Biology 2022Quote: ... The cDNA encoding mouse BANF1/BAF was synthesized using two120-mers and a 90-mer single-stranded DNA oligonucleotides (Table 2, Sigma-Aldrich). These ssDNA oligonucleotides each contain a 15-mer overlapping sequence at both ends ...
-
bioRxiv - Microbiology 2020Quote: ... stained for VZV glycoprotein E (gE) (MAB8612, Millipore) in BD PermWash ...
-
bioRxiv - Cell Biology 2019Quote: ... affinity purification for glycoproteins using Con A (Sigma) was performed overnight ...
-
bioRxiv - Neuroscience 2021Quote: ... myelin associated glycoprotein (#MAB1567; MAG; Sigma; 1:500), and the CSPG brevican (#SC-135849 ...
-
bioRxiv - Neuroscience 2023Quote: ... MOG (myelin oligodendrocyte glycoprotein, Cat#MAB5622, Merck Millipore) and NFH (neurofilament heavy chain ...
-
bioRxiv - Cell Biology 2023Quote: ... HEK293 cells stably expressing CCR5 were labeled with 2ug/ml rabbit anti-Flag tag antibody (F7425, Sigma, St. Louis, MO, USA) in FACS buffer (PBS with 0.5% bovine serum albumin (BSA) ...
-
bioRxiv - Microbiology 2024Quote: ... The 1 mL coronavirus suspension was then washed twice using AmiconUltra (Merck Millipore) with 10 mL phosphate buffer ...
-
bioRxiv - Immunology 2022Quote: ... and spike protein-bound antibodies were detected using an Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich, #A2429) at a 1:4000 dilution in blocking buffer ...
-
bioRxiv - Immunology 2022Quote: ... mice were immunized at weeks 0 and 4 with 5 µg of gH/gL monomer or 60-mer formulated in PBS (monomer) or TBS (60-mer) with 50% (v/v) Sigma Adjuvant System (SAS; Sigma Aldrich, catalog #S6322) for a total volume of 100 µL ...
-
bioRxiv - Developmental Biology 2022Quote: ... MAG (Anti-Myelin Associated Glycoprotein, clone 513 antibody, Millipore mab1567 ...
-
bioRxiv - Immunology 2023Quote: ... Antibody binding to S1 and RBD antigen was detected using a three-step approach consisting of anti-mouse or antirat Fc biotin (Sigma Aldrich B7401) followed by streptavidin-HRPO (Life Technologies cat# SA1007 ...
-
bioRxiv - Immunology 2021Quote: ... 20-mer gRNA sequences were introduced into primers (Millipore Sigma) and after hybridization were cloned into Esp3I-digested lentiGuide-puro using the NEBuilder® HiFi DNA Assembly Master mix (E2621 ...
-
bioRxiv - Immunology 2023Quote: ... 74-mer oligos containing a 5’ phosphate modification (Sigma-Aldrich) were ligated to form circular single-stranded DNA (ssDNA ...
-
bioRxiv - Biochemistry 2021Quote: HEK293 cells (Sigma:85120602) were cultured using DMEM (Gibco™ ...
-
bioRxiv - Biophysics 2023Quote: HEK293 cells (Sigma Aldrich) and were cultured in DMEM (Gibco ...
-
bioRxiv - Microbiology 2021Quote: ... both Bactrian camel and llama AEC cultures were treated with recombinant universal type I and III IFNs (100 IU/ml; Sigma Aldrich) or recombinant type III IFN (100 ng/ml ...
-
bioRxiv - Immunology 2021Quote: ... pH 6.5 and glycoproteins oxidized with 1.6 mM NaIO4 (Sigma) in PBS ...
-
bioRxiv - Plant Biology 2022Quote: ... equivalent blots were stained with Glycoprotein Detection Kit (Sigma-Aldrich) or probed with anti-GFP monoclonal Ab (SI sections 4 ...
-
bioRxiv - Immunology 2024Quote: ... a glycoprotein mucin standard derived from porcine stomach (Sigma-Aldrich) and BALF samples were diluted 2-fold serially with PBS ...
-
bioRxiv - Biochemistry 2023Quote: ... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
-
bioRxiv - Biochemistry 2021Quote: HEK293 and HEK293T cells (Sigma) were cultured at 37 °C ...
-
bioRxiv - Microbiology 2021Quote: ... for SARS CoV-2 spike detection mouse anti-spike (Cat# ZMS1076, Sigma Aldrich) respectively ...
-
bioRxiv - Biochemistry 2020Quote: Alpha-1-acid glycoprotein (AGP) purified from human serum (Sigma #G9885) and A/Philippines/2/1982 (Phil82 ...
-
bioRxiv - Immunology 2021Quote: ... The high mannose glycoproteins subsequently were treated with endoglycosidase F1 (Millipore) to obtain homogeneously deglycosylated RBD.
-
bioRxiv - Immunology 2023Quote: Myelin oligodendrocyte glycoprotein(MOG)-35-55 peptide(Sigma-Aldrich,100µg/ml), IFNγ(10ng/ml;Cat#315-05,PeproTech ...
-
bioRxiv - Biochemistry 2023Quote: ... The dried glycoproteins were reduced using 1,4-dithiothreitol (Sigma; 0.18 M) followed by carboxymethylation using iodacetamide (Sigma ...
-
bioRxiv - Biochemistry 2023Quote: ... Glycoproteins were digested with 1:25 Proteinase K (PK; Sigma-Aldrich) for 30 min at 37□C (For NVX-CoV2373 ...
-
bioRxiv - Bioengineering 2023Quote: ... mouse anti-P-glycoprotein (Sigma-Aldrich, cat# 517310, AB_564389, 1:100), rabbit anti-P-glycoprotein (Abcam ...
-
bioRxiv - Molecular Biology 2022Quote: ... k-mer sequences were assembled using PCR from oligos purchased from Sigma.
-
bioRxiv - Immunology 2023Quote: ... A 30-mer biotinylated single-stranded DNA ligand (0.025 μM, Sigma-Aldrich) was then immobilized onto the MC5 chip in FC2 through interaction with neutravidin ...
-
bioRxiv - Biochemistry 2023Quote: ... An 120-mer oligonucleotide containing the XBP1 intron was synthesized (Sigma Aldrich, sequence ...
-
bioRxiv - Genetics 2024Quote: The 69-mer reverse-phase cartridge-purified oligo ordered from Sigma-Aldrich were dissolved to 100 μM in TE buffer ...
-
bioRxiv - Cell Biology 2021Quote: Primary antibodies used are as follows: Anti-poly-ADP-ribose binding reagent/ PAR reagent (recombinant protein fused to rabbit Fc tag; Millipore, MABE1031), Rabbit monoclonal anti-Poly/Mono-ADP Ribose (anti-PAR/MAR ...
-
bioRxiv - Immunology 2021Quote: ... Complement reagent Serum (Sigma S1-100ml), Goat anti FH ...
-
bioRxiv - Genomics 2022Quote: ... The RevT primers (Table S1, Sigma) were dissolved in ultrapure water at a concentration of 100 μM and mixed at this concentration.
-
bioRxiv - Evolutionary Biology 2023Quote: ... and a S1 nuclease (Sigma-Aldrich) treatment along with an additional purification step (Zymoclean) ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were incubated with primary mouse IgG anti-coronavirus group antibody MAB9013 (Millipore Sigma; diluted 1:500) for 1 h at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... HEK293 WT (Sigma Cat. Nr. 85120602), HEK293 GALNT1 KO (Narimatsu et al. ...