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Citations for Millipore Sigma :
651 - 700 of 7883 citations for Lassa Fever Virus GP2 Protein Sheep Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Sections were washed in phosphate buffer saline with 0.5% Triton-x (PBST) and incubated with 20% heat-inactivated normal sheep serum in PBST for 2 hours (NSS; Sigma-Aldrich, Corp.). Primary antibodies were applied overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... UK) supplemented with 5% Defibrinated Sheep Blood (SR0051E, Oxoid, UK) with a cotton swab before placing Optochin disks (74042, Sigma-Aldrich, Germany) on the center of the plates and incubated at 37°C under microaerophillic conditions i.e ...
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bioRxiv - Neuroscience 2021Quote: ... brain sections were incubated for 30 minutes with a horse radish peroxidase (HRP)-conjugated sheep anti-DIG primary antibody (Sigma, catalog # 11207733910) diluted at 1/500 in Tris-NaCl blocking buffer (TNB ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections were incubated for 30 minutes with a horse radish peroxidase (HRP)-conjugated sheep anti-DIG primary antibody (Sigma, catalog #11207733910) diluted at 1/500 in Tris-NaCl blocking buffer (TNB ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were purchased: rabbit anti-PITPNB polyclonal antibody (pAb) and sheep anti-TGN46 pAb from Sigma-Aldrich (St. Louis, MO); rabbit anti-β-tubulin pAb from cell signaling (Danvers ...
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bioRxiv - Cancer Biology 2021Quote: ... Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase) of mouse or rabbit origin (Sigma-Aldrich) were used.
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bioRxiv - Molecular Biology 2020Quote: ... Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase) of mouse or rabbit origin (Sigma Aldrich) were used.
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in CF media + 37.5mg/ml 70 kDa Ficoll (Fc; Sigma, F2878) and 25 mg/ml 400 kDa Ficoll PM (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... Selective oxidation of hmC to fC was achieved using potassium perruthenate (KRuO4, Sigma-Aldrich) as described (Booth et al. ...
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bioRxiv - Neuroscience 2020Quote: ... 5% FCS with primary antibodies: anti-Calbindin D-28K (rabbit polyclonal, 1:1000, Millipore, AB1778 ...
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bioRxiv - Neuroscience 2021Quote: ... MaxiSorp plates (Thermo) were coated with 100µl of goat anti-human Fc antibody (Sigma) diluted 1:500 in PBS overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed before detection and goat anti-human Fc-HRP conjugated antibody (Sigma) (1:5000 dilution in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... 17 div cultured neurons were incubated with TrkB-Fc (1 µg/mL, T8694-Sigma) a BDNF scavenger suppressing BDNF binding to TrkB (Sakuragi et al ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% (vol/vol) FCS and 100 U/mL penicillin/streptomycin (Sigma Aldrich). D2.0R-GFP and 4T1-mCherry cells were generated by lentiviral transduction with TurboGFP and mCherry reporter ...
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bioRxiv - Cancer Biology 2022Quote: ... in RPMI-1640 medium complete (R8758, Sigma-Aldrich, supplemented with: 10% FCS (Sigma-Aldrich), 2mM L-glutamine (Biochrom) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 10% heat inactivated Fetal Calf Serum (FCS) (Sigma-Aldrich, St. Louis, Missouri, USA) and under standard culture conditions in humidified incubator at 37°C with 5% CO2.
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bioRxiv - Neuroscience 2020Quote: MaxiSorp plates were coated with 100 µl of goat anti-human Fc antibody (Sigma) diluted 1:500 in PBS or 3 mg/ml of an anti-VNAR antibody (courtesy of Martin Flajnik ...
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bioRxiv - Cancer Biology 2021Quote: ... consisting of 1% fetal calf serum (FCS; #S0615, Sigma, part of Merck, Darmstadt, Germany) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... binding was detected by using goat-anti-hIgG(Fc)-HRP (1:70000, A0170, Sigma). Binding was visualized by using TMB substrate ...
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bioRxiv - Microbiology 2022Quote: ... RPMI-1640 or MEM media and 10% Fetal Calf Serum (FCS, Sigma cat. F7524). THP-1 cells media was also supplemented with 0.05 mM β-mercaptoethanol (Euromedex ...
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bioRxiv - Microbiology 2021Quote: ... Cell permeabilization and blocking was performed in IF buffer (5% FCS, 0.05% saponin (Sigma) in PBS ...
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bioRxiv - Microbiology 2020Quote: ... and at day 13 with 50 μg of RBD-Fc plus Titermax adjuvant (Sigma). Blood were collected from individual mice one week after the last immunization (day 20 ...
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bioRxiv - Immunology 2021Quote: ... The cultures were supplemented on day 2 with FCS (10% v/v; Sigma-Aldrich). Medium was replaced every 3 days thereafter with fresh R+ containing FCS (10% v/v ...
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bioRxiv - Immunology 2020Quote: Cells were thawed and subsequently washed in IMDM+10%FCS+75U/ml DNAse (Sigma). To minimize day-to-day variation ...
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bioRxiv - Cancer Biology 2022Quote: ... 293T and NIH-3T3 cells were grown in DMEM with 10 % FCS (Sigma-Aldrich, St ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg/mL goat anti-human IgG1 Fc F(ab’)2 (Sigma-Aldrich, I3391) was coated in 96-well NUNC Immunoplate (Thermo Fisher ...
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bioRxiv - Physiology 2023Quote: Rm-EphrinA4/Fc chimera (R&D) was clustered with anti-human Cy3 antibody (Sigma) diluted 1 ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% Fetal Calf Serum (FCS, Capricorn Scientific) and 0.5% Neomycin (Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... and resuspended in a wash solution of 10% fetal calf serum (FCS, Sigma-Aldrich) in DMEM (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... Later the brain was transferred to a tube containing fluorinert (FC-40, Sigma-Aldrich) to reduce the background signal ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS) (Sigma Aldrich, F7524, St. Louis, MO, USA). Cytarabine-resistant cell lines were generated as described previously by us [15] ...
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bioRxiv - Developmental Biology 2023Quote: ... medium changed to IMDM+10%FCS+Penicillin-streptomycin + 8-bromo-cAMP (10μM, Sigma-Aldrich), and then incubated overnight at 37°C 5%CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... after which the cells were resuspended in PBS supplemented with 1% FCS (S7524, Sigma) and 2mM EDTA and kept at 4°C until the plate was measured in the flow cytometer ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant supplemented with 1% Pen/Strep and 10% FCS (Sigma-Aldrich, Missouri, USA) and stored in the dark at 4 °C.
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... UM cells were transduced with virus supplemented with 8 µg/ml polybrene (Sigma Aldrich, Saint Louis, Missouri, USA) and 24 h after transduction ...
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bioRxiv - Cell Biology 2020Quote: ... McArdle-RH7777 or IMR-90 were then transduced with the virus with 8 µg/ml polybrene (Sigma-Aldrich). After 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... Complementary DNA was reverse transcribed from 1 μg total RNA with Moloney murine leukemia virus reverse transcriptase (Sigma) using dNTP (Promega ...
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bioRxiv - Immunology 2021Quote: ... The swabs were placed into 2ml of virus transport medium (VTM) comprising tissue culture medium 199 (Sigma-Aldrich) supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 μl of pre-warmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Microbiology 2020Quote: ... the cell culture supernatants were pooled to concentrate the virus 20 times using 10,000 MWCO spin columns (Millipore), and aliquots were stored at −70°C [14] ...
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bioRxiv - Bioengineering 2020Quote: ... virus was collected after 120 hours from both cell lysates and media and purified over iodixanol (Optiprep, Sigma).
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bioRxiv - Biochemistry 2020Quote: ... P2 virus was then harvested by pelleting cells by centrifugation at 4000g and decanting into 5% FBS (Sigma), and then stored in the dark at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... the virus-containing medium was removed and replaced with selection medium containing 2 μg/ml Puromycin (Sigma–Aldrich). After 7 days of selection ...
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bioRxiv - Cell Biology 2022Quote: ... Infectious media containing virus was collected 40 hrs later and filtered with a 0.45μm PES membrane filter (Millipore). Live filtered virus was used to transduce cells with polybrene (10μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... Thiol concentration at the same intervals as cells infected with virus was determined by Ellman’s reagent (5,5’-dithio-bis-2-nitrobenzoic acid, DTNB, Sigma) by reading the optical density at 412 nm in a plate reader (Biotek Cytation 5 ...
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bioRxiv - Cancer Biology 2022Quote: ... the virus-containing supernatant was centrifuged 500 x g for 5min and filtered with 0.45um PES filters (Millipore), followed by ultracentrifugation (25,000rpm for 2h) ...
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bioRxiv - Immunology 2022Quote: ... the antibody-virus mixture was removed and 100 μl of pre-warmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Microbiology 2021Quote: ... E2 (CHIK-102 and CHIK-263) [42] or Zika Virus E as a control (ZV-2, Sigma-Aldrich) (kindly provided by Dr ...
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bioRxiv - Genetics 2020Quote: ... 10 μL of each virus was diluted in growth media containing polybrene (10 μg/mL; Sigma-Aldrich, USA) then mixed with cells ...
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bioRxiv - Genomics 2021Quote: ... We filtered and concentrated the virus in PBS using Amicon Ultra-15 centrifugation filters (Millipore, Burlington, MA; #UFC905024). The viral titer was measured with the AAVpro(R ...