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Citations for Millipore Sigma :
51 - 100 of 10000+ citations for Iron Sulfur Cluster Co Chaperone Protein HscB Mitochondrial HSCB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... treated with 1 M iron(III) chloride (Sigma-Aldrich) solution for 1 min.
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bioRxiv - Cancer Biology 2022Quote: ... iron-chelating agent Deferoxamine (DFO, Sigma-Aldrich, United States), protein synthesis inhibitor Cycloheximide (CHX ...
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bioRxiv - Systems Biology 2019Quote: ... iron chelators and DMSO (Cat No. 276855, Sigma-Aldrich) were diluted to 40 µM in 0.1 M sodium phosphate buffer pH 6.2/2% glucose ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mg/mL iron oxide nanoparticles (IONPs, Sigma 747467) was added to the acrylamide mixture ...
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bioRxiv - Pathology 2022Quote: ... supplemented with 10% iron fortified calf serum (Sigma-Aldrich) and 1% Penicillin/Streptomycin (P/S ...
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bioRxiv - Biochemistry 2023Quote: ... 13.3 mM ammonium iron(II) sulfate (Sigma-Aldrich, 09719), and 33.3 mM DTT ...
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bioRxiv - Microbiology 2022Quote: ... The iron content of pre and post Chelex® 100 treatment was determined using the Iron Assay Kit (Sigma-Aldrich, MA, United States) following the manufacturer’s recommendations (Table S5).
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... to 20 μg of mitochondrial protein and run with Tris-MOPS running buffer (#PCG3003; Sigma-Aldrich/Merck) on a 4-20% precast Trupage gel (#PCG2012-10EA ...
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bioRxiv - Cell Biology 2024Quote: ... Mitochondrial isolation was carried out by differential centrifugation using the Mitochondrial Isolation Kit (MITOISO2; Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Mitochondrial complex I inhibitor rotenone (Sigma), pan caspase inhibitor Z-VAD (OMe)-FMK (Abcam) ...
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bioRxiv - Cell Biology 2021Quote: ... Sigma mitochondrial isolation kit (Sigma, MITOISO1) was used to extract mitochondria and perform the JC-1 assay according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... mitochondrial uncoupler FCCP (1.5 μM, Sigma), and mitochondrial complex I and III inhibitors rotenone (100 nM ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were co-expressed in Rosetta 2(DE3)pLysS Ecoli (Novagen). Cells were grown at 37°C in 2XYT media to an OD600 of 0.8 ...
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bioRxiv - Bioengineering 2019Quote: ... and 1mM iron (II) sulfate (FeSO4) (Sigma-Aldrich, 215422-5G) (to 1L ...
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bioRxiv - Genomics 2021Quote: ... freshly prepared 10 μM Iron(II) sulfate hexahydrate (Sigma #203505), freshly prepared 10 mM carbamoylphosphate and 5 mM ATP ...
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bioRxiv - Pathology 2022Quote: ... Serial dilutions of ammonium iron (III) sulfate dodecahydrate (Sigma, #221260) were used as standard ...
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bioRxiv - Microbiology 2022Quote: ... while iron sufficiency was achieved by supplementation with FeSO4 (Sigma), hemin (Hm ...
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bioRxiv - Cancer Biology 2021Quote: ... doxycycline hyclate and Ammonium Iron (III) Citrate (FAC) from Sigma; aspartic acid from Acros ...
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bioRxiv - Evolutionary Biology 2019Quote: ... iron-free EDTA (Ethylenediaminetetraacetic acid disodium salt dehydrate, Sigma-Aldrich) was added to reach a total EDTA concentration of 20 μM ...
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bioRxiv - Physiology 2021Quote: ... ferritin (iron) from equine spleen – Type I standard (Sigma Aldrich) was diluted to 100 ...
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bioRxiv - Microbiology 2022Quote: ... 500 μM iron (II) chloride tetrahydrate (FeCl2, Sigma-Aldrich, 44939), 100 μM cobalt chloride (CoCl2 ...
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bioRxiv - Bioengineering 2022Quote: ... Sections were soaked in Weigert’s Iron Hematoxylin (HT1079, Sigma-Aldrich) solution for 10 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... Sections were soaked in Weigert’s Iron Hematoxylin (HT1079, Sigma-Aldrich) solution for 10 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... or iron (III) sulfate hydrate (Sigma-Aldrich, St. Louis, US), chosen for their common occurrence in wastewater and their known interactions with antibiotics ...
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bioRxiv - Microbiology 2023Quote: ... 150 µM iron(II) sulfate heptahydrate (Sigma-Aldrich, F7002-250G), and 100 µM calcium chloride dihydrate (Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and Certified Multielement Ion Chromatography Anion Standard Solution for Bromine and Sulfur quantification (10 mg l-1, TraceCERT, Sigma-Aldrich) were also used to calibrate these respectively elements ...
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bioRxiv - Physiology 2022Quote: ... Membranes was incubated with the primary antibodies against target proteins: rabbit polyclonal anti-TSG 101 (T5701, Sigma-Aldrich Co., 1:200), rabbit polyclonal anti-CD63 (SAB4301607 ...
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bioRxiv - Biophysics 2021Quote: ... and DNAJB1 chaperone variants were concentrated on an Amicon Ultra-15 10K molecular weight cutoff (MWCO) filter (Millipore) and further purified on a HiLoad 16/600 Superdex 200 pg gel filtration column (GE Healthcare) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged as clusters with collagenase IV (Sigma) when reaching approximately 70% confluence ...
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bioRxiv - Cell Biology 2020Quote: Mitochondrial membrane potential was measured by observing mitochondrial accumulation of the fluorescent dye tetramethylrhodamine ethyl ester (TMRE; Sigma), as previously described [38 ...
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bioRxiv - Cell Biology 2022Quote: ... Mitochondrial respiration was determined by exposing MCF7s treated with MSC-EVs to mitochondrial perturbing agents Oligomycin (10μM, Sigma), FCCP (6μM ...
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bioRxiv - Immunology 2019Quote: ... Protein concentration was determined using BCA protein assay reagent (Pierce Chemical Co.) with bovine serum albumin (Sigma) as the standard.
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bioRxiv - Cancer Biology 2020Quote: ... Iron chelation was performed with DFO (Sigma-Aldrich, St. Louis, MO) in concentrations ranging from 0-50µM added to SFM for 24-72 hours depending on the experiment.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 10 mmol of iron(III) chloride hexahydrate (FeCl3.6H2O; Sigma Aldrich, UK) and 10 mmol trans ...
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bioRxiv - Bioengineering 2021Quote: ... Solution of 30 mM ammonium iron (II) sulfate hexahydrate (Sigma-Aldrich) was used as an iron source and added to the reaction vessel at a constant rate of 211 μl/min at an excess of about 5000 Fe atoms per ferritin cage ...
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bioRxiv - Physiology 2021Quote: ... cell nuclei were stained in Weigert’s Iron Hematoxylin Solution (Sigma Aldrich) and darkened in running tap water ...
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bioRxiv - Cell Biology 2021Quote: ... an Iron (III) Immobilised Metal Chelate Affinity Chromatography (IMAC) resin (Sigma). Desalted peptides were resuspended in 30% MeCN ...
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bioRxiv - Neuroscience 2022Quote: ... Hydrated slides were first stained with Weight’s Iron Hematoxylin (Sigma, UK) working solution 7 mins and gently washed with running tap water for 10 mins to remove excessive stain ...
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bioRxiv - Neuroscience 2023Quote: ... 4.17 µL of 100 mg/mL iron(II) sulphate (FeSO4, Sigma) and 301.5 µL of 1 M magnesium chloride (MgCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Thermo Fisher 033316.30) or iron chelators (2,2’-Bipyridyl, Sigma Aldrich D216305), in which the compound was mixed with bacteria prior to seeding ...
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bioRxiv - Molecular Biology 2023Quote: ... Mitochondrial uncoupler CCCP was purchased from Sigma and applied directly to yeast cultures to a final concentration of 20μM from stock solutions in DMSO.
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bioRxiv - Microbiology 2022Quote: ... Iron concentrations were then normalized to the protein content in each sample using the bicinchoninic acid (BCA) assay (Sigma Aldrich, St. Louis, MO). Quantification was performed from 3 independent cultures for each strain.
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bioRxiv - Microbiology 2020Quote: ... The mitochondrial import assay results were analysed by visualizing the proteins with the Coomassie stain (PhastGel Blue R, Sigma). The proteins were then fixed (20% ethanol and 10% acetic acid) ...
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bioRxiv - Cancer Biology 2021Quote: ... Co-Immunoprecipitation was performed using PureProteome™ Protein A Magnetic Beads (Millipore, LSKMAGA10). Protein extracts were first precleared using 10μl of beads for 200μg of proteins (1-hour incubation at 4°C) ...
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bioRxiv - Pathology 2019Quote: ... Co-IP assays were performed by incubating total proteins with anti-HA (Sigma) agarose followed by column wash and elution according to manufacturer’s instructions (Pierce ...
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bioRxiv - Microbiology 2022Quote: ... Iron deficiency was obtained by the addition of 2,2’-dipyridyl (DP) (Sigma) to the medium ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then immersed in Weigert’s iron hematoxylin solution (Sigma Aldrich, #HT1079) for 5-10 min (depending on freshness) ...
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bioRxiv - Cell Biology 2019Quote: Recombinant human holo-lactoferrin (iron-saturated) (L1294) was purchased from Sigma-Aldrich. Aliquots were prepared by resuspending in PBS at a concentration of 1 mg/ml and were stored at −20 °C ...
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Necrosis reduction efficacy of subdermal biomaterial mediated oxygen delivery in ischemic skin flapsbioRxiv - Bioengineering 2022Quote: Calcium peroxide (CaO2, FMC Corporation), iron (II, III) oxide (Fe3O4, Sigma-Aldrich), sodium bicarbonate (NaHCO3 ...
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bioRxiv - Physiology 2022Quote: Total iron levels were measured by a colorimetric assay (MAK025-1KT, Sigma) according to the manufacturer’s instructions.