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Citations for Millipore Sigma :
1 - 50 of 9083 citations for Inhibin beta C chain INHBC Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Immunology 2022Quote: ... Antibody production was conducted by co-transfection of the heavy and light chain expression vectors into Human HEK293 suspension culture cells (Expi293F) using polyethylene mine (Sigma). Cells were subsequently cultured for 4 days before being pelleted by centrifugation ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney 293 cells (HEK293) (Sigma), HEK293T (TakaraBio) ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK293) cells were maintained in a 37°C/5% CO2 atmosphere in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; human α-syn: 804-258-1001, Enzo Life Science; beta-actin: A4700, Sigma). After incubation with the first antibody ...
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bioRxiv - Microbiology 2020Quote: ... anti-human IgM (μ-chain specific, Sigma, St. Louis, MO) was covalently coupled to NHS HP SpinTrap (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-human IgG (γ-chain specific, A9544, Millipore-Sigma) or IgA (α-chain specific ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-human IgG (γ-chain specific, A9544, Millipore-Sigma) or IgA (α-chain specific ...
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Cell Biology 2024Quote: HEK293 (Human Embryonic Kidney, ECACC 96121229 from Sigma-Aldrich Chemie GmbH) were cultured in DMEM (Dulbecco’s modified Eagle’s medium PAN biotech ...
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bioRxiv - Immunology 2022Quote: ... containing 2% (vol/vol) of heat inactivated (HI, for 1h at 56°C) human AB serum (hABS) (Sigma, #H3667). After extensive washings with DPBS (Gibco ...
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bioRxiv - Immunology 2021Quote: ... secondary antibody (goat anti-human γ-chain-specific whole IgG, α-chain-specific IgA, or μ-chain-specific IgM alkaline phosphatase conjugate, Sigma Aldrich, MO, US) was diluted 1:1000 and 50μl was added per well for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... 1:3000 dilution of anti-human IgA (α-chain-specific) (Sigma) or 1:3000 dilution of anti-human IgM (μ-chain-specific ...
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bioRxiv - Neuroscience 2022Quote: ... 4 μM Cytosine beta-D-arabinofuranoside hyrdrochloride (Ara-C; Sigma) was supplemented into the media on either 1 day in vitro(DIV ...
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bioRxiv - Immunology 2021Quote: ... or 1:3000 dilution of anti-human IgM (μ-chain-specific) (Sigma) antibody subtypes and 1:6000 dilution of anti-human IgG1 ...
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bioRxiv - Microbiology 2022Quote: ... and HEK293-ACE2 cells [HEK293 cells (ATCC CRL-1573) stably expressing human ACE2]22 were maintained in DMEM (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... monoclonal antibodies were produced by transient co-transfection of 293-F cells with human heavy chain and light chain antibody expression plasmids using polyethylenimine (PEI) (Sigma-Aldrich, catalog #408727). Seven days after transfection ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Physiology 2020Quote: ... and human C-peptide (Millipore) were detected by ELISA kits in the plasma samples collected during the human insulin or human C-peptide challenges ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were harvested and Fab fragment was purified via the heavy chain His tag using affinity chromatography with Talon resin as per the manufacturer’s specifications (Sigma). Purified protein samples were dialysed into PBS as quantified by absorbance at 280nm.
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bioRxiv - Cell Biology 2024Quote: ... was added to the cell pellet and stored at −20°C prior to being assayed for insulin using human insulin specific RIA (Millipore Cat# HI-14K).
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bioRxiv - Biochemistry 2022Quote: ... and another was loaded with 10 ng of human HSP70 (His tagged human HSP70, SRP5190, Millipore Sigma), which allowed comparisons to be made among separate gels ...
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bioRxiv - Neuroscience 2020Quote: ... Cultured human embryonic kidney 293 (HEK293) cells were transfected using 2 μg/μl polyethylenimine (Sigma-Aldrich) with indicated plasmids for the expression of either Flag-KCTDs alone or in combination with Flag-Cav2.3 ...
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bioRxiv - Neuroscience 2019Quote: ... and PAK/cofilin mechanism experiments used human embryonic kidney cells (HEK293) cultured in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
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bioRxiv - Plant Biology 2023Quote: ... in-frame of the C-terminal (His)6-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a:ApTPS1 ...
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bioRxiv - Biophysics 2021Quote: His-tagged human PCNA protein was purchased from Sigma Aldrich (catalogue number SRP5117), at a concentration of 6.6 μM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Bioengineering 2019Quote: ... human tenascin-C (Millipore Sigma, Catalog #CC065), and human plasma fibronectin (Millipore Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... c) IgG from human serum (Sigma-Aldrich, resuspended in deionised H2O and stored at 4°C) ...
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bioRxiv - Systems Biology 2021Quote: Dispersed human islets and iPSC-beta cells were exposed to 0.5 mmol/L palmitate (Sigma-Aldrich) for 24 h and 48h ...
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bioRxiv - Biochemistry 2021Quote: HEK293 cells (Sigma:85120602) were cultured using DMEM (Gibco™ ...
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bioRxiv - Biophysics 2023Quote: HEK293 cells (Sigma Aldrich) and were cultured in DMEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... + beta-Mercaptoethanol (Sigma). Samples were denatured for 5 min at 95°C and gel was run using a 4-20% Mini-PROTEAN ® TGX precast protein gels (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... beta-mercaptoethanol (Sigma), and 10 ng/mL FGF2 (Peprotech) ...
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bioRxiv - Cell Biology 2024Quote: ... beta (A1978, Sigma), anti-acetylcholine esterase (AChE ...
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bioRxiv - Microbiology 2021Quote: ... Bound antibodies were detected with Peroxidase conjugated goat anti-human kappa light chains antibody (A7164-1ML, Sigma). 100 ul TMB(Tetramethylbenzidine,Biopanda TMB-S-001 ...
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bioRxiv - Immunology 2022Quote: ... 100 μL of 1:5000 diluted Goat anti-human secondary antibody was added into each well and incubated at 37°C for 1 hr (Goat Anti-Human IgG γ Chain Specific HRP conjugated, species Adsorbed (Human IgM, IgD, and IgA) Polyclonal Antibody for IgG (Cat# AP504P, EMD Millipore, Burlington, MA), Goat Anti-Human IgA ...
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bioRxiv - Immunology 2020Quote: ... For IgM antibody detection biotinylated anti-human IgM (affinity purified, μ-chain specific, Sigma-Aldrich cat. Nr. B1140) was used as secondary antibody.
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bioRxiv - Immunology 2020Quote: ... For IgM antibody detection biotinylated anti-human IgM (affinity purified, μ-chain specific, Sigma-Aldrich cat. Nr. B1140) was used as a secondary antibody ...
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bioRxiv - Immunology 2023Quote: ... AP-labeled goat anti-human IgG (γ-chain specific; Sigma-Aldrich, Vienna, Austria; 1:50,000 in TBS BSA) and AP-labeled goat anti-human IgA (α-chain specific ...
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bioRxiv - Cell Biology 2024Quote: ... respectively (Millipore Cat# HI-14K, HI-13K). Samples treated with 3 mM glucose KRBH were measured undiluted ...
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bioRxiv - Biochemistry 2021Quote: HEK293 and HEK293T cells (Sigma) were cultured at 37 °C ...
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bioRxiv - Microbiology 2020Quote: The human embryonic kidney cell lines HEK293 and HEK293T were cultured in Dulbecco’s modified Eagle’s medium (D1152, Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: U2OS osteosarcoma cells and HEK293 (human embryonic kidney) cells were grown in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma, D1152) supplemented with 10% fetal bovine serum (FCS ...
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bioRxiv - Neuroscience 2019Quote: ... beta-actin (Sigma-Aldrich), MAO-B (GeneTex) ...
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bioRxiv - Physiology 2021Quote: ... and Beta-Actin (Sigma), washed in TBST ...
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bioRxiv - Plant Biology 2021Quote: ... Beta-estradiol (Sigma-Aldrich) was solubilized in 100% ethanol to 5 mg/mL stock concentration and added to ½MS media during preparation of solid media to a final concentration of 2.5 μg/mL ...
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bioRxiv - Plant Biology 2021Quote: ... Beta-estradiol (Sigma-Aldrich) was solubilized in 100% ethanol to 5 mg/mL stock concentration and added to ½MS media during preparation of solid media to a final concentration of 2.5 μg/mL ...
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bioRxiv - Plant Biology 2022Quote: ... Beta-estradiol (Sigma-Aldrich) was solubilized in 100% ethanol to 5 mg/mL stock concentration and added to 1/2MS media during preparation of solid media to a final concentration of 2.5 µg/mL.
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bioRxiv - Genetics 2020Quote: ... 5µM beta-mercaptoethanol (Sigma) and 1mM sodium pyruvate (Life Technologies) ...