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Citations for Millipore Sigma :
451 - 500 of 5781 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 8 µg/mL polybrene (Sigma #H9268), 10ng/mL mIL3 (Peprotech #213-13) ...
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bioRxiv - Cell Biology 2023Quote: ... BAPTA-AM (126150-97-8, Millipore) at 25 μM final concentration ...
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bioRxiv - Molecular Biology 2023Quote: ... phospho-RPA2 (Ser4/8) (Sigma, PLA0071).
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bioRxiv - Microbiology 2023Quote: ... 8% dextran sulfate (Sigma-Aldrich, #D8906), 0.34mg/mL E ...
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bioRxiv - Molecular Biology 2023Quote: ... 8-Br-cAMP (Cat. B5386; Sigma), MPA (Cat ...
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bioRxiv - Microbiology 2023Quote: ... 8-methoxy-psoralen (Sigma, M-3501) was added to a final concentration of 0.33 mg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... 8 mg EDTA (Sigma-Aldrich, E6758) and 8 mg L-Cystein (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... 8 μg/ml protamine sulfate (Sigma) and 10 μM HEPES (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... cytokeratin 8 (1:100; MABT329 Millipore), phospho-histone H3 S10 (1:300 ...
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bioRxiv - Neuroscience 2023Quote: ... ICI-118,551 (Sigma, 72795-01-8) were dissolved in DMSO (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Sigma)/8% acrylamide (AA, 40%, A4058, Sigma) diluted in PBS was added to achieve a final concentration of 4%FA/4%AA ...
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bioRxiv - Cell Biology 2023Quote: ... 8 ng/ ml Cholera Toxin (Sigma), 2×10-11 M Tri-iodothyronine (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... 8-Br-cAMP (#B7880, Sigma-Aldrich) was used at 0.5-1 mM concentration for 5 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... 8 μg/ml polybrene (EMD Millipore) was added and cells were spun for 90 minutes at 800 x g at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... and 8% gelatin (Sigma-Aldrich, G2625). Sectioning of the samples was accomplished at a thickness of 100 µm using a Leica CM3050 cryostat equipped with low-profile blades (Leica ...
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bioRxiv - Microbiology 2022Quote: For immunofluorescence experiments the panel of HEK293 cells grown on poly-Lysine (Sigma-Aldrich Cat. Nr. P6282) pre-coated (2.5 μg/mL in MQ H2O 30 min at room temperature (RT) ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 and HeLa cells were grown in Dulbecco’s modified Eagle’s medium (DMEM)-GlutaMAX™ -I (Sigma-Aldrich) supplemented with 10% fetal bovine serum (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... HEK293 cells (2 x 104 cells per well) were transiently transfected using X-tremeGENE™ 9 (Sigma) with plasmids encoding eYFP tagged PAR2 (PAR2-eYFP ...
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bioRxiv - Biophysics 2021Quote: ... and HEK293 control cells were separated by SDS-PAGE using TruPAGE precast gels (4-12%) (Sigma-Aldrich) or 8% Laemmli SDS-gels ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were co-transfected with plasmids with AKT-PH::GFP and shRNA against KIAA1109 (Sigma-Aldrich, TRCN0000263343 with 73% knockdown efficiency) ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells from ATCC (ATCC# CRL-1573) cells were cultured in DMEM cell culture media (Sigma-Aldrich) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... IL-1Rα and IL-10) were analyzed by multiplex assays using custom multiplex cytokine plates (EMD Millipore, Billerica, MA, USA). Data were collected on a MAGPIX instrument (Luminex ...
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bioRxiv - Immunology 2024Quote: ... or alternatively plus IL-4 (4ng/ml) or IL-4 and lipopolysaccharide (LPS) (10μg/ml) (Sigma-Aldrich, St. Louis, MO) for 2.5 days.
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bioRxiv - Cancer Biology 2020Quote: The growth curve assay was performed using the Cell Counting Kit-8 (CCK-8) (Sigma-Aldrich, #96992) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... Endo-(±)-α-(Hydroxymethyl)benzeneacetic acid 8-methyl-8-azabicyclo[3.2.1]oct-3-yl ester (atropine) and 8-Cyclopentyl-1,3-dimethylxanthine (CPT) were from Sigma. Picrotoxin from Indofine Chemical Company (Hillsborough ...
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bioRxiv - Cancer Biology 2023Quote: ... MPC3 cells were infected 8 h with lentivirus in the presence of 8 μg/mL polybrene (Millipore). After 48 h ...
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bioRxiv - Molecular Biology 2021Quote: ... and 80 ng/ml IL-2 (Sigma-Aldrich) for their activation ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 100 U/ml IL-2 (Sigma).
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bioRxiv - Genomics 2019Quote: ... along with IL-2 (30 U/ml; Sigma). When indicated ...
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bioRxiv - Neuroscience 2023Quote: ... anti-mouse IL-6 ELISA kit (Sigma, RAB0309), anti-mouse G-CSF ELISA kit (MCS00 ...
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bioRxiv - Neuroscience 2024Quote: ... Cytokine (TNF-α, IL-1α, C1q-Sigma-Aldrich) stock solutions (1000X ...
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bioRxiv - Biochemistry 2022Quote: ... as a His-tag fusion and Ars2206-531 into pRSFduet vector (Novagen) as a Strep-tag fusion ...
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bioRxiv - Molecular Biology 2019Quote: ... Monoclonal anti-His and anti-Flag antibodies were obtained from Sigma Aldrich, St ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Buffer G and allowed to go overnight at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Lysis/Binding Buffer (50 mM Na2HPO4 ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified over HIS-Select (Sigma–Aldrich, St. Louis, MO) nickel affinity resin in 20 mM Tris (pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... 10 % heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich), and 1 % antibiotic-antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... The supernatant was loaded onto His-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with buffer A (25 mM Tris–HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Bound protein was detected using anti-His-HRP conjugate (Sigma; 1:10,000). All proteins and antibodies were in 0.1 M phosphate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The β-Spectrin-His construct was transformed into BL21(DE3) cells (Novagen) and induced for protein expression with 0.5mM IPTG (Invitrogen ...
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bioRxiv - Immunology 2022Quote: 30µL/well of rabbit anti-His tag antibody (Sigma Aldrich, catalog #SAB5600227) was adsorbed at a concentration of 0.5 μg/mL on to 384 well microplates (Thermofisher ...
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bioRxiv - Bioengineering 2022Quote: ... Pre-cleared supernatant was mixed with Ni-NTA His-Bind Resin (Novagen) (Pajenda et al. ...
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bioRxiv - Biochemistry 2022Quote: ... His6-tagged proteins were purified with Ni-NTA His-Bind Superflow (Novagen) and dialyzed against PBS using a Pur-A-Lyzer™ Midi Dialysis Kit (Merck).
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bioRxiv - Immunology 2020Quote: ... supplemented with 15% FBS HI (Life Tehnologies) + 1% Pen/Strept (Sigma-Aldrich), 1% L-glutamine (Life Technologies) ...
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bioRxiv - Immunology 2022Quote: The recombinant proteins were purified using His•Bind® Purification Kit (Novagen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Purification of rcCBP2 was performed by HIS-Select HF Nickel Affinity (Sigma) and gel permeation chromatography on Superdex 75 column (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes followed by anti-his antibody (Sigma, Cat. No. 11922416001) for another 10 mins ...
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bioRxiv - Microbiology 2021Quote: ... The crude lysate rocked with HIS-select nickel affinity gel resin (Sigma) for 45 min at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was probed with 1:10000 anti-His antibody (Sigma, USA) followed by 1:20000 anti-mouse antibody (Sigma ...