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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for IL 3 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... HIS-tagged B subunit of Shiga toxin 1(SML0655) and Apilimod (A149227) from Sigma; rabbit anti-EEA1 (#3288) ...
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bioRxiv - Plant Biology 2021Quote: ... Western blotting was performed using anti His-tag (Cat no. H1029-5ML, Sigma, USA) as well as with anti GST antibody (Cat no ...
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bioRxiv - Plant Biology 2020Quote: ... The pET-26-tNPR1-His plasmid was then transformed into Rosetta (DE3) pLysS (Novagen). The overnight culture at 37°C was transferred to fresh LB medium containing 50 μg/mL kanamycin and was incubated to OD600=1.0 at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich) and 1% antibiotic-antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham, MA or Anti-HA; Sigma Aldrich, St.Louis, MO) was added to the blocking solution at 1:2,000 dilution and incubated overnight at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... and the subsequent western blot analyses were performed with anti-His (Sigma, Missouri, USA) or anti-FLAG antibody (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% (v/v) HI-FBS and 1% (v/v) antibiotic/antimycotic (Sigma) in a standard tissue culture incubator at 37 °C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... A horseradish peroxidase-labeled anti-His tag antibody (1:25000 dilution; Sigma-Aldrich, Poland) diluted in 5% skimmed milk / TBS-Tween (0.1% ...
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bioRxiv - Neuroscience 2020Quote: ... The His-tag was removed by O/N incubation with TEV protease (Millipore Sigma) at 4 °C followed by negative INAC ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were detected using monoclonal anti-polyHis-HRP conjugate (Sigma-Aldrich, CAD). Phosphorylated mitogen-activated protein kinases (MAPK ...
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bioRxiv - Immunology 2020Quote: ... and loaded onto a cOmplete™ His-Tag Purification Resin gravity column (Sigma Millipore). Resin was first washed with detergent-containing buffer (25 mM Tris + 500 mM NaCl + 0.5 % N-Dodecyl-β-D-maltoside ...
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bioRxiv - Immunology 2022Quote: ... The lysate was mixed gently with 50% Ni-NTA His-bind slurry (EMD Millipore) at 4:1 ratio on a shaking platform for 60 minutes at RT ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was validated by microarray using monoclonal anti-polyhistidine (clone His-1, Sigma).
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bioRxiv - Cell Biology 2019Quote: ... input was taken and 20 µl HIS-Select® HF Nickel Affinity Gel (Sigma) pre-equilibrated with Pull-Down Buffer was added to each sample and incubated on an overhead rotator for 1h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and the supernatant was incubated with 6 ml HIS-Select Nickel Affinity Gel (Sigma), gently agitated at 4°C for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were detected by immunoblotting with primary mouse anti-His (Sigma□Aldrich, Taiwan) or anti-GST primary antibodies (Sigma□Aldrich ...
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bioRxiv - Immunology 2020Quote: ... BM cells were cultured in growth media supplemented with 10% HI FBS (Sigma-Aldrich), 1% Pen/Strep (Lonza) ...
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bioRxiv - Immunology 2020Quote: ... and loaded onto a cOmplete™ His-Tag Purification Resin gravity column (Sigma Millipore). Resin was first washed with detergent-containing buffer (25 mM Tris + 500 mM NaCl + 0.5 % N-Dodecyl-β-D-maltoside ...
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bioRxiv - Biochemistry 2021Quote: ... the His-tag was removed by treating purified nanobody Nb_0 with carboxypeptidase A (Sigma) and B (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... Expression of pAgos was verified by Western blot with antibodies against His-tag (Sigma) after cell growth for 2 hours at 30 °C ...
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bioRxiv - Bioengineering 2022Quote: ... and mVenus-SpyCatcher-6×His were prepared using Gibson assembly with pET-19b (Novagen) as the plasmid vector ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mpp75Aa1.1 or Mpp75Aa1.1-His was mixed with high purity pepsin (Sigma, St. Louis, MO) in 2 mg/ml NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS-hi; Sigma, Cat. no. F7524), 1% antibiotic-antimycotic (AA ...
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bioRxiv - Biochemistry 2024Quote: ... and dilutions were as follows (in parenthesis): His (mouse, Sigma cat# H1029; 1:2,500); β-actin (rabbit ...
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bioRxiv - Neuroscience 2020Quote: ... DAB (3-3’-diaminobenzidine, Sigma-Aldrich catalog no. D8001-10G) was dissolved in 0.1 N HCl at a concentration of 5.4 mg ml-1 and subsequently diluted ten-fold into sodium cacodylate buffer (pH 7.4 ...
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bioRxiv - Developmental Biology 2022Quote: ... The reaction was developed using 3-3’-Diaminobenzidine (DAB) (Sigma). For CLASP1 immunostaining ...
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bioRxiv - Cancer Biology 2019Quote: ... 3-hydroxybutyrate (3-OHB) was also purchased from Sigma (#166898).
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bioRxiv - Biochemistry 2021Quote: ... 2-amino-3-phosphonopropionic acid (AP-3, Sigma-Aldrich A4910), ATP (Sigma-Aldrich A2383) ...
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bioRxiv - Cell Biology 2019Quote: ... injected with 3 mL of 3% thioglycollate (Sigma-Aldrich, T9032). After 3 days ...
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bioRxiv - Immunology 2020Quote: ... E-64 and 3-Methyladenine (3-MA) were from Sigma. Necrostatin-1 was from Abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC; Sigma Aldrich #39391), and 3NPH solutions were mixed ...
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bioRxiv - Neuroscience 2023Quote: ... (3) the PDE4B inhibitor A33 (3 mg/kg, Sigma-Aldrich), (4 ...
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bioRxiv - Immunology 2024Quote: ... 3-nitropropionic acid (3-NP, several doses, Sigma Aldrich N5636), Atpenin-A5 (several doses ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 10 mg/mL 3-iodo-L-tyrosine (3-IY, Sigma), and 0.5 mM all-trans-retinal for 2–3 days at 25°C in the dark prior to behavioral testing ...
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bioRxiv - Cell Biology 2020Quote: ... and containing 125 pg/mL recombinant IL-15 (Sigma-Aldrich) to aid uNK cell maturation ...
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bioRxiv - Neuroscience 2020Quote: E-4031 (Alomone-Labs, IL) and TEA (Sigma-Aldrich, USA) were dissolved in 0.9% saline ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich), and 0.5 μg/ml anti-CD180 (RP/14 ...
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bioRxiv - Bioengineering 2022Quote: ... 0.1mM ascorbic acid and 50 pg/mL IL-1β (Sigma).
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bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/ml mouse recombinant IL-4 (Sigma-Aldrich) to allow B cell activation and class switch recombination to IgG1 ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Neuroscience 2020Quote: ... 3% BSA (Sigma), 0.3% Sodium azide (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... Nutlin-3 (Sigma) was made up to 20mM in DMSO and used at 5µM ...
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bioRxiv - Neuroscience 2020Quote: ... 3 (Sigma Aldrich). For the immunofluorescence staining of freshly extracted zfPGCs ...
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bioRxiv - Microbiology 2020Quote: ... 3% milk (Sigma). Primary antibody detecting ...
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bioRxiv - Cancer Biology 2022Quote: ... 3% BSA (Sigma) and 0.1% Tween-20 (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mixture (Sigma). Cell lysates were then centrifuged at 15000×g for 20 min ...